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Citations for Thermo Fisher :
4251 - 4300 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse-transcribed to cDNA using oligo(dT) primers and M-MuLV reverse transcriptase (Thermo Fisher Scientific). PCRs were run with PrimeSTAR GXL DNA Polymerase (Takara ...
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bioRxiv - Immunology 2019Quote: ... M-MØ (1 × 106 cells) were transfected with AhR-specific siRNA (siAhR) (50 nM) (# s1199, Thermo-Fisher Scientific), using HiPerFect (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... A TR-FAME column (length: 30 m, inter diameter: 0.25 mm, film size: 0.25 μm, 260M142P, Thermofisher Scientific) was used with helium as carrier gas ...
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bioRxiv - Bioengineering 2020Quote: ... One liter of 0.2 M phosphate buffer contained 20.44 g of Na2HPO4 and 6.72 g of NaH2PO4 (Acros Organics). For sample dehydration ...
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bioRxiv - Biophysics 2020Quote: To immobilize above prepared DNA constructs to streptavidin coated microspheres (Thermo Fisher Dynabeads M-270 2.8 μm diameter), we took 20 μl of streptavidin microspheres and washed thrice with 50 μl of phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... Cells were harvested by centrifugation and suspended in 400 µl of 4 M guanidine isothiocyanate solution (Invitrogen, UK). DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: ... Twenty micrograms of each peptide were incubated with 20 µl of pre-washed Dynabeads M-280 Streptavidin (Invitrogen) at RT for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... followed by thermal oxidation in a tube furnace (Lindberg/Blue M 1200°C Split-Hinge; Thermo Scientific, USA) at 800 °C for 1 h in ambient air ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 μg plasmid and 1.5 μl Fugene HD (m/v=1:3) were diluted and incubated in 0.25 ml OptiMEM (Gibco). After 5 min ...
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bioRxiv - Microbiology 2021Quote: ... and cDNA was generated by retrotranscription of 1 μg of total RNA using M-MLV reverse transcriptase (Invitrogen) and random primers (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... these samples were subjected to a second purification step using 30 μl M-280 Streptavidin Dynabeads (Invitrogen 11205D). The rest of the steps were performed as previously described94 ...
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bioRxiv - Immunology 2019Quote: ... 0.5 × 106 BMDMs/sample were lysed with 0.1% TritonX-100 Sucrose-Tris (0.25 M sucrose, 40mM Tris [pH 7.4]) lysis buffer with protease and phosphatase inhibitors (ThermoFisher). For the NADase assay on intact macrophages ...
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bioRxiv - Neuroscience 2019Quote: ... We purified biotin-containing proteins with Dynabeads (150 μl per reaction; Dynabeads M-280 Streptavidin-Thermo Fisher Scientific). Dynabeads were separated over a magnetic rack and subsequently washed 2 times in PBS containing 0.1% Bovine Serum Albumin (BSA) ...
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bioRxiv - Neuroscience 2021Quote: ... each monkey received an injection of a bidirectional tracer conjugated with dextran amine (40–50 nl, 10% in 0.1 M phosphate buffer, pH 7.4; Invitrogen). Twelve days after the injection ...
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bioRxiv - Neuroscience 2020Quote: ... Serial coronal sections (20 μm) of the brain were obtained using a cryostat (Microm 560 M, Thermo Fisher). Tissue was first incubated with a blocking solution containing PBS ...
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bioRxiv - Genomics 2021Quote: ... 0.4 vol glycerol (100% solution), 0.12 vol 2X dialysis buffer (100 mM HEPES-KOH at pH 7.2, 0.2 M NaCl (Invitrogen, AM9759), 0.2 mM EDTA (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested by addition of 100μl of 0.05 M ABC containing 0.3 μg of trypsin (90057, Thermo Scientific) at 37°C for 16 hours ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of total RNA was used for cDNA synthesis using the M-MLV reverse Transcriptase Kit (Invitrogen) and an oligo(dT ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted and 2 μg RNA was transcribed into cDNA with M-MLV reverse transcriptase (Invitrogen) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... To arrest cells in M phase they were treated with 1:100 KaryoMAX Colcemid solution (15212012; Gibco, UK) in PBS for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... The gonads were removed and placed in 1 M phosphate buffer saline (PBS) with 1x streptomycin penicillin (Gibco). They were then rinsed and subsequently cut into pieces of about one millimeter ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were separated across a 2.1 m□×□5 cm C18 separating column (1.9 mm Hypersil Gold, Thermo Fisher) with a linear gradient of 4-40% CH3CN and 0.3% formic acid over 6 min ...
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bioRxiv - Neuroscience 2022Quote: ... The slides were washed in 0.1 M PB and cover-slipped using Prolong Gold Antifade (Fisher Scientific P36930). Images were taken on Olympus FluoView 1200 confocal microscope or Olympus FluoView 3000 confocal microscope ...
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bioRxiv - Bioengineering 2022Quote: ... Silk was dried and subsequently dissolved in 9 M LiBr (199870025, Acros, Thermo Fisher Scientific, Breda, The Netherlands), filtered ...
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bioRxiv - Genomics 2022Quote: ... Cells were passaged by incubation in 0.5 mM EDTA-PBS (stock: 0.5 M EDTA, AM9260G, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... then incubated for 30-45 m with goat anti-mouse Alexa Fluor 555 (Invitrogen cat#A32727, 1:1000) or goat anti-rat Alexa Fluor 488 (Invitrogen cat#A11006 ...
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bioRxiv - Microbiology 2022Quote: ... First stage PCR amplification was carried out using DreamTaq Green PCR master mix (M/s Thermo scientific, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) respectively supplemented with proteinase and phosphatase inhibitors (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... before conducting immunoprecipitation based on the instructions of the M-280 Sheep anti-Rabbit IgG Dynabeads (11203D, Invitrogen). The mRNA with m6A enrichment was assayed using RT-qPCR ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 300 ng of total RNA using the M-MLV Reverse Transcriptase (ThermoFisher Scientific). SYBR® Green JumpStart™ Taq ReadyMix™ (Merck ...
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bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s protocol including the options to add 20 μl of 2 M dithiothreitol (Life Technologies/Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... in phosphate buffer (0.1 M, pH 7.0) before inspection using a TalosTM transmission electron microscope (TEM) (ThermoFisher, USA).
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bioRxiv - Neuroscience 2023Quote: ... Antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit or mouse IgG (Thermo Fisher Scientific) for 6h at 4°C in the following day ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed in 0.1 M PB and cover-slipped using Prolong Gold Antifade (Thermo Fisher Scientific). Images were taken using an Olympus FluoView 3000 confocal microscope and analyzed using Cellsens (Olympus ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragmented nascent RNA was purified using 30 μl of Streptavidin M-280 magnetic beads (Thermo Fisher Scientific) and ligated with 3’ RNA adapter (5’p-GAUCGUCGGACUGUAGAACUCUGAAC-/3InvdT/) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μg of total RNA was used to synthesized cDNA using the M-MLV reverse transcriptase (Life Technologies) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... The supernatant was separated and incubated with 10 µL of Dynabeads M-450 anti-mouse IgG (Invitrogen #11041) for 10 minutes ...
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bioRxiv - Biophysics 2022Quote: ... CD4 and CCR5 overexpressing HeLa cells (gift of David M. Rekosh, University of Virginia) were maintained in Iscove’s Modified Dulbecco’s Medium (Gibco) supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic with 0.5 mg/mL of G418 (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: Primary human fibroblasts with or without mutant mitochondrial DNA (m.3243G) were routinely maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) containing 25 mM glucose ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then reduced and alkylated with 1 μL of 0.5 M tris-2(-carboxyethyl)- phosphine (ThermoFisher) and 5 μL of 0.4 M chloroacetamide in a 90 °C water bath for 10 min followed with 5 min bath sonication ...
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bioRxiv - Immunology 2023Quote: ... and 40 µL per mL of complex of 1 M formaldehyde prepared from 37 % stock (Thermo Fisher, 10630813). The complex was incubated for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... Annie Newell-Fugate at Texas A&M University) were cultured in Eagle’s minimal essential medium (EMEM; Fisher Scientific) using the same supplements and environments described for HFF culture ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Cancer Biology 2024Quote: ... The biotinylated RNA-RNA complexes were pulled down by incubation with Dynabeads M-280 Streptavidin (Thermo Fisher Scientific) for 4 hr at 40C ...
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bioRxiv - Genomics 2024Quote: ... and 2 μL of Dynabeads M-280 magnetic beads (Sheep Anti-Mouse IgG, Sheep Anti-Rabbit IgG, Invitrogen) using the SX-8G IP-Star Automated System (Diagenode) ...
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bioRxiv - Biophysics 2024Quote: ... The resulting peptide-mRNA-DNA fusions were then incubated with PfUCLH3 immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... the PBS was aspirated and cells were lysed in complete M-PER buffer (Thermo Scientific, Rockford, IL, USA). Complete M-PER buffer was prepared through addition of Protease & Phosphatase Inhibitor Cocktail (1X final ...
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bioRxiv - Cell Biology 2023Quote: ... Protein from S and P fractions were mixed with 2 µL 0.5 M DTT and 4x LDS (Invitrogen), incubated at 70°C for 10 minutes and run on NuPAGE 10% Bis-Tris gels in MES/SDS-PAGE running buffer (1M MES ...
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bioRxiv - Evolutionary Biology 2022Quote: ... full MS/MS scans (375–1500 m/z) were performed in the Orbitrap mass analyser (Thermo Fisher Scientific) with a 60,000 resolution at 200 m/z ...
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bioRxiv - Molecular Biology 2022Quote: ... the pH was lowered to 8 by addition of 1 M Tris base and monobromobimane (mBBr, Life technologies) was added in excess to derivatize the CoASH ...