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Citations for Thermo Fisher :
4201 - 4250 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... This was followed by staining with mouse-absorbed Alexa 488-conjugated goat anti-rat antibody (Molecular Probes) diluted 1:50 with PBS ...
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bioRxiv - Genomics 2024Quote: ... the slides were incubated with an anti-rat secondary antibody AF488 (diluted 1:400, A11006, Thermofisher scientific) for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used: 488 Alexa Fluor donkey anti-rat (Invitrogen, A-21208, 1:500), 555 Alexa Fluor donkey anti-rabbit (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated them overnight in rat anti-mCherry antiserum (1:2500; Thermo Fisher, Product#: M11217, Lot#: 2695213) and rabbit anti-CGRP antiserum (1:2500 ...
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bioRxiv - Systems Biology 2024Quote: ... and Alexa Fluor™ Plus 555 goat anti-rat (Thermo Scientific, cat no: A48263, 1/250 dilution). Nuclear signal was detected using DAPI (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... 2 μg of total RNA was used for reverse transcription with M-MLV Reverse Transcriptase (Thermo Fisher Scientific). SYBR Green PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Cells were directly lysed in the wells using appropriate volume of M-PER mammalian protein extraction reagent (Thermofisher) containing 1x Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Immunology 2020Quote: ... onto an Acclaim PepMap RSLC analytical column (75μ m × 50 cm, nanoViper, C18, 2μm, 100 Å; ThermoFisher Scientific). The peptides were separated by increasing concentrations of 80 % ACN/0.1 % FA at a flow of 250 nL/min for 158 min and analyzed with an Orbitrap Fusion™ Tribrid™ mass spectrometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: For each sample and IgG control: 120 μl of Dynabeads M-280 Sheep anti-mouse IgG (Thermo Fisher) were pre-blocked overnight at 4°C on a wheel in 1 ml of PBS supplemented with 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription was performed using M-MLV Reverse Transcriptase and random primers following manufacturer’s instructions (Thermo Fisher Scientific). Gene-specific primers were designed over different exons to prevent amplification of genomic DNA ...
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bioRxiv - Molecular Biology 2021Quote: Phf14 antibody (proteintech 24787-1-AP) was coupled to M-280 Sheep Anti-Rabbit IgG Dynabeads (Invitrogen 11203D) according to manufacturer’s instructions overnight (7 µL antibody ...
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bioRxiv - Bioengineering 2019Quote: ... The 10X S750 salts consist of (filter sterilized): 0.5 M MOPS (free acid) (Fisher Scientific, cat no. ICN19483725), 100 mM of ammonium sulfate (NH4)2SO4 (Millipore Sigma ...
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bioRxiv - Genomics 2019Quote: cDNA synthesis was performed from 2 μg of total RNA using M-MLV Reverse Transcriptase (Invitrogen 28025-013) and random primers ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleared lysate was incubated with 100 µl pre-equilibrated magnetic IgG-coupled Dynabeads M-270 Epoxy (Life Technologies) for 1 h at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell suspension was filtered through a 70 μm cell strainer and centrifuged at 1200 rpm for 5 min and the pellet was re-suspended in 0.9 M sucrose in 0.5 x HBSS (Gibco). After further centrifugation for 20 min at 2000 rpm ...
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bioRxiv - Genetics 2019Quote: ... CD90 APC (4μl, 559869, 5E10), CD7 A700 (10μl, 561603, M-T701) and Streptavidin Conjugate Qdot 605 (3μl, ThermoFisher, Q10101MP). Cell sorting was performed on the FACSAria III (BD Biosciences) ...
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bioRxiv - Genomics 2020Quote: ... Sonicated chromatin was diluted in 1X TBST+PI (25 mM Tris-Cl pH 8.0, 0.15 M NaCl, 0.05% Tween-20, and 1X Pierce Protease Inhibitors [Thermo Fisher]). For input libraries ...
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bioRxiv - Genomics 2021Quote: The cDNAs were synthesized from DNaseI-treated total RNA with M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA, USA) and Oligo (dT ...
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bioRxiv - Genomics 2021Quote: ... followed by addition of 100 µl of the stop solution (0.05 M H2SO4; Thermo Fisher Scientific, Waltham, USA). Absorbance was read at 450 nm using a microplate reader (xMark ...
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bioRxiv - Genomics 2021Quote: ... Cells were resuspended in 500 μl Wash Buffer (47.5 ml H2O RNAse free, 1 ml 1 M HEPES pH 7.5 (Invitrogen), 1.5 ml 5M NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... hippocampi were washed three times in HBSS (HBSS, 1 M HEPES, penicilline/streptomycine, 100 mM sodium pyruvate; Gibco) and were dissociated via trypsin digestion (2.5% ...
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bioRxiv - Bioengineering 2020Quote: ... the container was quickly transferred to a vacuum oven set to 240 °C (Lindberg/Blue M, Thermo Scientific) and degassed at 25 inHg for 5 min ...
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bioRxiv - Bioengineering 2020Quote: ... culture media was collected and cells were lysed in M-PER® mammalian protein extraction reagent (Thermo Scientific). Cell media from 4 replicates was pulled as a single sample for analysis using a mouse inflammation antibody array (RayBio® ...
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bioRxiv - Neuroscience 2021Quote: ... we injected the fluorophore BODIPY TMR-X into MOs (Invitrogen; 5 mM in PBS 0.1 M, DMSO 40%). After 3 hours ...
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bioRxiv - Neuroscience 2020Quote: HA or GSAP antibody was covalently conjugated to Dynabeads® M-270 Epoxy beads (#14301, Thermo Fisher Scientific) using the antibody coupling kit (#14311D ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in Urea buffer (8 m Urea, 50 mm ammoniumbicarbonate, Halts Protease-phosphatase-inhibitor (Thermo Scientific)) ...
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bioRxiv - Neuroscience 2019Quote: ... The crosslinking reaction was stopped with 1/10 volume of 1.25 M glycine pH 8 (Thermo Fisher Scientific). Crosslinked cell pellets were washed 3 times with ice-cold DPBS followed by lysis or snap freezing and storage at −80°C ...
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bioRxiv - Plant Biology 2021Quote: ... Metabolites were separated with a 30 m TG-5MS column (Thermo Scientific, 0.25mm i.d. 0.25 um film thickness) and detected using a Perkin Elmer Clarus 690 GC coupled to a Clarus SQ 8S mass spectrometer (Perkin Elmer) ...
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bioRxiv - Biochemistry 2020Quote: ... and 200 U of M-MLV reverse transcriptase using the RT2 First Strand cDNA Synthesis Kit from (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Embryonic mouse hippocampus was dissected at E17.5 in ice-cold 0.02 M HEPES in Ca2+/Mg2+-free HBSS (Gibco) and primary cell suspensions dissociated via incubation with 2.5 mg/ml trypsin (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... 1×106 cells were lysed in 50 μL of M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Systems Biology 2021Quote: ... were cleaned by sonication in 1 M sodium hydroxide and 100% ethanol in Ultrasonic Cleaner (Fisher Scientific FS20) three times for 10 minutes each ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was stopped by ethanol precipitation: 1 μl 3 M RNAse-free sodium acetate (pH 5.5) (Ambion), 1 μl RNAse-free glycogen (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... a mixture of 10% 10,000 MW BDA and 3% 3,000 MW BDA in 0.01 M sodium phosphate buffer; Molecular Probes, Invitrogen) was injected ...
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bioRxiv - Physiology 2021Quote: ... Complementary DNAs (cDNAs) were synthesized from 25 ng/μL of total RNA using M-MLV Reverse Transcriptase (Invitrogen) or water (for negative RT ...
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bioRxiv - Neuroscience 2022Quote: Mice were deeply anaesthetized with isoflurane (Baxter Medical AB) and perfused transcardially with 25ml of 0.125 M PBS (pH 7.4, Gibco) and 40 ml of 4% paraformaldehyde (PFA ...
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bioRxiv - Bioengineering 2022Quote: Green food dye (McCormick Corp.) was added to solutions of 0.1 M of KSCN (Thermo Fisher Scientific Corp.) and 0.05 M of Fe(NO3)3 (Thermo Fisher Scientific Corp. ...
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bioRxiv - Microbiology 2022Quote: ... and subjected to detergent exchange with 0.1 M MES buffer pH 6.5 complemented with 0.1% lauryldimethylamine-oxide (LDAO) detergent (Affymetrix, UK) and 25 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were finally washed twice for 10 min in 0.1 M PB (pH 7.4) prior to mounting on glass slides using ProLong Gold Antifade reagent (Invitrogen). All references for primary and secondary antibodies can be found in Table 2 ...
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bioRxiv - Physiology 2022Quote: ... HPAEC lysates were collected in 1X M-PERTM Mammalian Protein Extraction Reagent from Thermo Scientific (Waltham, MA, USA) spiked with protease/phosphatase inhibitors examined by immunoblotting ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were rinsed with Phosphate Buffered Saline (PBS, Gibco, 10010023, pH 7.4 at 25°C, 0.01 M) to remove any residual medium and an excess of additives that did not penetrate inside the cells ...
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bioRxiv - Neuroscience 2021Quote: ... 400 µg protein were incubated with 40 µL of streptavidin coupled DynabeadsTM M-280 (Thermo Fisher Scientific, #11205D). After 1 hr of incubation at room temperature on a rotating wheel ...
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bioRxiv - Neuroscience 2021Quote: ... Three 5-min TBS and two Tris buffer (0.1 M Tris base, pH 7.5) washes were performed before sections were mounted in Prolong Gold (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... A 5μL Hamilton syringe attached to a 30-gauge needle was used to inject Cholera Toxin subunit B conjugated to AlexaFluor 488 (CTB-488, 0.5 % diluted in 0.1 M phosphate buffer saline [PBS] pH 7.4; ThermoFisher Scientific) at three sites into the bladder wall (3 μL/injection ...
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bioRxiv - Neuroscience 2021Quote: ... the washing medium was removed and 80 L of 5 M cell permeant Fluo-4 AM (Life technologies) in neurobasal medium was added to wells at room temperature and incubated at 37 °C for 30 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... Approximately 5 μg of the digested total RNA was reverse-transcribed using the M-MLV reverse transcriptase (Invitrogen) and random primers (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were rinsed with phosphate-buffered saline (PBS, Gibco, 10010023, pH 7.4 at 25°C, 0.01 M) to remove any residual medium and an excess vitamin C that did not penetrate inside the cells ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were washed three times for 10 minutes (0.01 M PBS) and mounted with Fluoromount (Thermofisher, USA). Images were collected in a Leica microscope.
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bioRxiv - Cancer Biology 2022Quote: ... biotinylated PCR product is bound and isolated using Dynabeads M-280 Streptavidin magnetic beads (Thermo Fisher Scientific, #11206D). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... pH 4.7) and precipitated in ethanol with 0.3 M sodium acetate and 1 µl linear acrylamide (AM9520, Ambion). RNA was then subjected to a decapping reaction with 2 units of Cap-Clip acid pyrophosphatase (C-CC15011H ...