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Citations for Thermo Fisher :
4101 - 4150 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Physiology 2023Quote: ... Chromatin complexes were precipitated with 100µl Dynabeads M-280 (sheep anti-rabbit #11203D; Thermo Scientific, Waltham, MA). After washes and elution ...
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bioRxiv - Microbiology 2023Quote: ... cultures were diluted 1:400 on filtered NaCl (0,9 M) in 96-well plates (Nunc, Thermo Scientific) and analysed with a CytoFLEX-S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted 1:400 on filtered NaCl (0,9 M) in 96-well plates (Nunc, Thermo Scientific) and analysed with a CytoFLEX-S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted 1:400 on filtered NaCl (0,9 M) in 96-well plates (Nunc, Thermo Scientific) and analysed with a CytoFLEX-S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was dissolved in 50 μL of 6 M guanidine hydrochloride (GuHCL, Thermo Scientific™, AAJ6078622) and placed on the shaker for 20 min at 1000 rpm ...
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bioRxiv - Developmental Biology 2023Quote: ... and complementary DNA (cDNA) was made from 500 ng using M-MLV Reverse Transcriptase (Invitrogen 28025-013) and dN6 primers ...
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bioRxiv - Cell Biology 2024Quote: ... After capture of the products on washed Streptavidin beads (10 μl, Dynabeads M-280; Thermo Fisher, #11205D), the DNA was denatured with 150 mM NaOH for 5 min at 21°C 2 times ...
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bioRxiv - Molecular Biology 2024Quote: ... and briefly centrifuged for 1 minute at 1000 rpm using a M-20 rotor (Thermo Scientific, 75003624) in a tabletop centrifuge (Legend X1R ...
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bioRxiv - Neuroscience 2024Quote: ... 2 M urea in 50 mM Tris-HCl (pH 7.5) with 1 mM dithiothreitol (DTT, Thermo Fisher) and 5 µg/ml trypsin (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of total RNAs were reverse transcribed using random hexamers and M-MLV reverse transcriptase (Invitrogen). Quantification of gene expression was performed by qPCR using Itaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was performed using either Superscript™ II or M-MLV reverse transcriptase according to manufacturer’s protocols (Invitrogen, Waltham, Massachusetts, USA, https://www.thermofisher.com/Invitrogen). Real-time reverse transcription polymerase chain reaction was performed using a QuantStudio™ 3 Real-Time PCR System or a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... slides were incubated with secondary goat anti-rat IgG (H+L) Alexa Fluor 488 (1:500; Invitrogen) and goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... BM endothelial cells were immunopurified from cell suspensions using sheep anti-rat IgG Dynabeads (ThermoFisher Scientific 11035) pre-captured with a CD31 antibody (MEC13.3 ...
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bioRxiv - Cancer Biology 2022Quote: ... as primary antibody and Alexa Fluor® 647 goat anti-rat (dilution 1/200, Invitrogen, cat. A21247) as the secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... the slides were incubated with the following secondary antibodies (anti-rat IgG Alexa Fluor 594, Molecular Probes A-11007 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in goat anti-rat Alexa 568 secondary antibody (1:500, #A11077, ThermoFisher, RRID: AB_2534121) and donkey anti-rabbit horseradish peroxidase conjugated secondary antibody (1:500 ...
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bioRxiv - Genomics 2019Quote: ... as a secondary antibody for the GAPDH antibody and Goat anti-Rat IgG (H+L) (Invitrogen 31470) as a sec-ondary antibody for the CTD antibody ...
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bioRxiv - Immunology 2019Quote: ... followed by goat-anti-rat secondary antibody-Alexa Fluor® 800 conjugate (0.33 ng/ml; ThermoFisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... rat monoclonal α-Elav 1:10 (Developmental Studies Hybridoma Bank) and rabbit α-GFP 1:500 (Invitrogen).
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies used were anti-mouse or anti-rat Alexa 568 at 1:400 (Thermo Fisher Scientific). Nuclei were labeled using 0.05 μg/ml DAPI (Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: ... and anti-E-cadherin (rat, 1:50; formaldehyde-hand peeled fixation; DHSB) antibodies and AlexaFluor568 phalloidin (Invitrogen) were used ...
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bioRxiv - Cancer Biology 2019Quote: ... This was followed by incubation with Alexa555-conjugated anti-rat and Alexa488-conjugated anti-mouse (Molecular Probes). DNA fibers were studied in a LSM780 confocal microscope using a 64x oil objective and lengths of CldU and IdU tracks were measured using the ImageJ software (http://fiji.sc/Fiji) ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used were: Alexa Fluor 488 anti-mouse and Alexa Fluor 555 anti-rat (Fisher Scientific, ref.A11029 and ref ...
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bioRxiv - Cell Biology 2021Quote: ... 105 cells/ml were re-suspended in 500 µl of 2.3 mgs/ml collagen rat tail (Gibco). A 100 µl volume of the collagen and cell mixture was dispensed in quadruplicate wells onto poly-D-Lysine (0.1mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... rat and rabbit IgG or chicken IgY are obtained from Invitrogen (A-32723, A-11034, 1:300) or Jackson ImmunoResearch Laboratory (711-165-152 ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa-Fluor goat anti-rabbit 680 and Alexa-Fluor goat anti-rat 800 (all from Life Technologies). The membrane was imaged using the LiCor Imaging System and Odyssey V3.0 software (LiCor) ...
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bioRxiv - Neuroscience 2020Quote: ... Followed-by Goat anti-Rat IgG (H+L) Secondary Antibody HRP (Thermo Scientific, USA, # 31470 1:5,000), Goat anti-Rabbit IgG H&L (IRDye® 800CW ...
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bioRxiv - Cell Biology 2021Quote: ... alone or together with Alexa Fluor 647-conjugated goat anti-rat secondary antibody (A-21247, Invitrogen, USA) or Alexa Fluor 647-conjugated goat anti-rabbit secondary antibody (A-21245 ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (Thermo Fisher Scientific, #A11081, 1:1000), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Physiology 2022Quote: ... -rat and –guinea pig secondary antibodies at RT for 1 hour on a nutator (1:500, ThermoFisher, #A21206 ...
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bioRxiv - Neuroscience 2022Quote: ... and for 48 hours for staining with rat α NG2 (1:250; Thermo Fisher Scientific: MA5-24247). Other primary antibodies used include guinea pig α Vglut2 at 1:1000 (Sigma ...
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bioRxiv - Physiology 2022Quote: ... Diluted primary antibody (100 µL of 1:200 rat anti-rabbit a-tubulin, Clone YL1 / 2, Invitrogen) was pipetted onto the Parafilm and the coverslips were placed cell-side down on top of the solution and incubated at 4 °C for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Flat mounts were washed and incubated in Alexa Fluor 488 (1:500, goat anti-rat, Invitrogen, A110006) for up to 2 hours on a shaker at room temperature ...
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bioRxiv - Immunology 2021Quote: The INS-1 rat insulinoma cells were cultured at 37°C in RPMI-1640 (Thermal Fisher Scientific) containing 11.2 mM glucose and 2 mM L-glutamine and supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from individual rat retinas using TRIzol® RNA extraction reagent (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or goat anti-rat IgG conjugated to Alexa Fluor 488 (1:500; Cat. No. A-21212, Invitrogen) and donkey anti-mouse IgG conjugated to Alexa Fluor 555 (1:500 ...
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bioRxiv - Physiology 2021Quote: Rat neonatal cardiomyocytes were isolated from P3 pups using the Pierce primary cardiomyocyte isolation kit (Thermo Scientific). The neonatal cardiomyocytes were seeded on (50 μg/ml ...
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bioRxiv - Evolutionary Biology 2020Quote: 120-150-µm thick sections of live rat tibia bones were obtained by vibratome (Thermo Fisher Scientific) and mounted onto glass discs using silicone grease from the Bruker fluid cell accessory kit (Bruker ...
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bioRxiv - Biochemistry 2020Quote: Rat L6 myoblasts were maintained in α-minimum essential medium (α-MEM) containing 5.5 mM glucose (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... from E17 C57BL/6 mice and primary rat cortical neurons (RCNs; Thermo Fisher Scientific, cat. no. A36512) from E18 Sprague Dawley rats were thawed and cultured according to the manufacturer’s recommendations in a B-27 Plus Neuronal Culture System (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2022Quote: ... followed by a rat anti-mouse IgG conjugated to biotin (Cat#13-4013-85, ThermoFisher, Basel, Switzerland) and a streptavidin conjugated Alexa546 (s11225 ...
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bioRxiv - Neuroscience 2019Quote: ... rat anti-MHC class II 1:500 (14-5321-82, eBioscience, Thermo Fisher Scientific, San Diego, CA), goat anti-mannose receptor 1:500 (AF2535 ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were incubated with CldU secondary antibody (goat anti-rat AF647, ThermoFisher A-21247; Lot# 1696458, 200538) or IdU secondary antibody (goat anti-mouse AF 546 ...
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bioRxiv - Bioengineering 2020Quote: ... Transwells were coated with rat tail collagen I (Gibco, A10483-01, 50 µg mL-1 in PBS) for 1-2 hours in the incubator ...
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bioRxiv - Microbiology 2020Quote: ... and rat (Rattus norvegicus, accession number NM_001012006.1) were cloned into a modified pcDNA3.1-cmyc/C9 vector (Invitrogen) as previously described (37 ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies used were: Alexa-488 and Alexa-568 conjugated anti-rat antibody (Molecular Probes; 1:200), Alexa-488 and Alexa-568 conjugated anti-mouse antibody (Molecular Probes ...
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bioRxiv - Developmental Biology 2020Quote: ... Mouse or Rat conjugated with Alexa Fluor 488 or Alexa Fluor 647 fluorophore secondary antibodies (Life Technologies) were used at 1:500 in PBTA solution.
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bioRxiv - Genetics 2021Quote: ... and soaked in a 1:500 dilution of primary antibody (rat anti-tubulin, Invitrogen Cat #MA1-80189) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were transferred to a 35mm dish and submerged in neutralized rat tail collagen I (Life Technologies). Collagen solidified for 30 min at 37 °C ...