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Citations for Thermo Fisher :
4351 - 4400 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Mass spectrometry data acquisition: Analysis of TMT labeled peptide digests was carried out on an Orbitrap Q Exactive HF-X instrument from Thermo Fisher Scientific (Bremen ...
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bioRxiv - Cell Biology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Genetics 2019Quote: ... Approximately 1 μg of peptides from each digested band was analysed using LC-MS/MS on a Q Exactive mass spectrometer (ThermoFisher Scientific), as previously described [66].
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bioRxiv - Genetics 2020Quote: ... The resulting peptides were fractionated using a Dionex Ultimate 3000 nanoHPLC system in line with an LTQ-Orbitrap Velos mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Biophysics 2021Quote: ... SARS-CoV-2 ORF10 protein (full lenth, crude, Mass spectrometry data of crude peptides is included in supplementary information) was purchased from Thermo Scientific, USA.
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bioRxiv - Cancer Biology 2020Quote: ... The second half of the data (runs 5-8) was obtained after peptide separation on 75 μm columns packed with 2.6 μm Accucore beads (Thermo Fisher Scientific). Peptides were injected onto 30-40 cm ...
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bioRxiv - Immunology 2022Quote: ... The concentrated peptides were injected into in a Q Exactive HFX instrument (Thermo-Scientific) connected to an Easy-nLC 1200 instrument (Thermo Scientific). The peptides were analyzed in data-dependent mass-spectrometry (DDA-MS ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min acetonitrile gradient ...
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bioRxiv - Neuroscience 2022Quote: ... Eluted peptides were dried down overnight before resuspension in 5% formic acid ready for injection onto the Orbitrap Exploris (Thermo Fisher) mass spectrometer using data independent acquisition (DIA) ...
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bioRxiv - Biochemistry 2022Quote: ... Mass recalibration for each sample was determined manually by performing a separate search on each raw file for linear peptides in Proteome Discoverer 2.4 (Thermo Fisher Scientific) without spectrum recalibration ...
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bioRxiv - Biophysics 2022Quote: ... faecalis was purified as described previously55 and fluorescently labeled at the stem peptide lysine with Alexa Fluor 488 NHS ester (Life Technologies). For labeling ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried peptides were dissolved in 0.1% TFA and analysed by LC-MS/MS on Q Exactive plus mass spectrometer (Thermo Fisher Scientific). Peptide identification was performed using the Mascot search engine (Casado and Cutillas ...
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bioRxiv - Biophysics 2022Quote: ... The cross-linked peptides were separated on a PepMap RSLC column (250 mm × 75 μm, 2 μm particle size, ThermoFisher Scientific). Chromatographic separation was performed with a 60 min gradient starting at 6% and increasing to 40% B (mobile phase A ...
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bioRxiv - Cancer Biology 2022Quote: Peptide samples were analyzed on a Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Rockford, IL, USA) coupled with a high-performance liquid chromatography system (EASY nLC 1200 ...
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bioRxiv - Biochemistry 2022Quote: ... Synthetic peptides were coupled to rabbit serum albumin (RSA) with the linker Sulfo-m-maleimidobenzoyl-N-hydroxysuccinimide ester (Sulfo-MBS, Thermo Scientific) in a carrier (1):linker (50):peptide (50 ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Biophysics 2022Quote: ... Peptides were directly injected over the course of a 75 min acquisition into a Q-Exactive Plus mass spectrometer (ThermoFisher Scientific). Raw MS data were searched against the Uniprot canonical isoforms of the human proteome using the default settings in MaxQuant (version 1.6.12.0) ...
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bioRxiv - Cancer Biology 2022Quote: The resulting peptides were analyzed using a nanoAcquity liquid chromatography (Waters) coupled with a Q Exactive HF-X (Thermo fisher scientific). Samples were analyzed randomly ...
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bioRxiv - Cell Biology 2022Quote: ... 1,000 ng of peptides from each biological replicate were first injected onto an using an Ultimate3000 high-performance liquid chromatography system (ThermoFisher Scientific) coupled online to an Orbitrap HF-X (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Digested peptides were diluted to 60 μL with water and labeled for 1 hour at room temperature using 16plex TMTpro (Thermo Scientific) or 10plex TMT (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted peptides were introduced into the mass spectrometer (Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer, Thermo Fisher Scientific, USA). The spray voltage was set at 2.2 kV and the heated capillary at 270°C ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides eluted from the capillary column were applied directly onto a Q Exactive Plus mass spectrometer by electrospray (Thermo Fisher Scientific) for mass spectrometry (MS ...
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bioRxiv - Immunology 2022Quote: ... The sites of phosphorylation of 32P-labeled peptides were also determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer (Kyoto, Japan) with the peptides coupled to an 80 Sequelon-AA membrane (Applied Biosystems), as described previously (Campbell and Morrice ...
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bioRxiv - Plant Biology 2022Quote: ... The peptides were initially trapped on a C18 PepMap100 pre-column (300 µm i.d. x 5 mm, 100 Å, Thermo Fisher) using solvent A (0.1% formic acid in water) ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting peptides were collected on a trap column (2 mm × 2 cm) with POROS 20 R2 material (Thermo Scientific) at 0.5°C ...
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bioRxiv - Microbiology 2022Quote: The raw LC-MS/MS data was analyzed against the Uniprot Gallid alphaherpesvirus 2 database (taxon 10390; 1300 sequences) using the Byonic peptide search algorithm (Protein Metrics) integrated into Proteome Discoverer 2.4 (Thermo Fisher Scientific). Optimal main search settings were initially determined with Byonic Preview (Protein Metrics ...
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bioRxiv - Microbiology 2022Quote: ... The peptides were analyzed by capillary LC-tandem mass spectrometry using an Orbitrap Fusion Lumos tribrid ion trap mass spectrometer (ThermoFisher Scientific). The resulting MS/MS data was processed using Sequest HT search engine within Proteome Discoverer 2.5 SP1 against a Homo Sapiens protein database obtained from Uniprot (78 787 entries ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Molecular Biology 2023Quote: Separation of peptides produced by AspN digestion was performed on a ICS3500 nano-UHPLC system (Thermo Fisher Scientific/Dionex, Germering, DE)) employing a 75 μm x 150 mm Easy spray column packed with C-18 reverse phase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The peptides were analyzed by liquid chromatography (LC)-MS/MS (PROXEON coupled to a QExactive HF mass spectrometer, Thermo Fisher Scientific) with three injections of 5 μl digests ...
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bioRxiv - Neuroscience 2023Quote: ... 25 μg of peptide labeled with TMT reagent (0.4 mg, dissolved in 40 μl anhydrous acetonitrile, Thermo Fisher Scientific, Cat # 90111) and made at a final concentration of 30% (v/v ...
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bioRxiv - Physiology 2023Quote: ... An aliquot (10%) was removed for quantification using the Pierce Quantitative Fluorometric Peptide Assay kit (Thermo Fisher Scientific, Cat. No. 23290). The remainder of the peptides from each IP samples (∼0.5-3.5 µg ...
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bioRxiv - Plant Biology 2023Quote: ... A total of 2 μg of re-suspended peptide was injected per replicate using Easy-nLC 1200 system (LC140; Thermo Scientific) and an Acclaim PepMap 100 C18 trap column (Cat# 164750 ...
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bioRxiv - Neuroscience 2023Quote: ... Eluted peptides were lyophilized and reconstituted in 300 µL 0.2% TFA for fractionation using the Pierce High pH Reversed-Phase Peptide Fractionation Kit following the instructions provided by Thermo Fisher Scientific for TMT-labelled samples ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Molecular Biology 2022Quote: Peptides were analysed by reversed-phase liquid chromatography on an EASY-nLC 1000 or 1200 system (Thermo Fisher Scientific, Odense, Denmark) coupled to a Q Exactive plus or HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: The prepared tryptic peptide sample of each subject was injected individually into an Ultimate3000 Nano/Capillary LC System (Thermo Scientific, UK) coupled to a Hybrid quadrupole Q-Tof impact II™ (Bruker Daltonics ...
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bioRxiv - Immunology 2022Quote: EAE was induced in 8-12 week old mice by subcutaneous injection with 100 μL of inoculant containing 100μg myelin oligodendrocyte glycoprotein peptide fragment 35-55 (MOG35-55, Thermo Scientific J66557MCR) in PBS with complete Freund’s adjuvant (CFA ...
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bioRxiv - Molecular Biology 2022Quote: ... The dried peptides were re-dissolved with 0.1% FA solution for LC-MS analysis (Q-Exactive, Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Plant Biology 2022Quote: ... Five microliters of peptide mixtures were analyzed in duplicate on a Q-Exactive Plus hybrid quadripole-orbitrap mass spectrometer (Thermo Fisher) as described in Pérez-Pérez et al ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated on an Ultimate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) with a linear gradient reaching 55% MS solvent B after 85 min ...
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bioRxiv - Molecular Biology 2022Quote: ... TMT labeled peptides were analyzed by nano-ultrahigh performance (UHP)LC–MS/MS (Easy-nLC1200, Orbitrap Fusion LumosTribrid, Thermo Fisher Scientific). Proteome Discoverer 2.2 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Peptides were extracted from brains from 5 d old male flies and analyzed on an Orbitrap Fusion mass spectrometer (Thermo Scientific) equipped with a PicoView ion source (New Objective ...
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bioRxiv - Biochemistry 2024Quote: Fractionated crosslinked peptides were loaded onto a Vanquish Neo HPLC coupled to a nano-ESI source of an Orbitrap Eclipse (ThermoFisher Scientific). Samples were injected onto a 300μm x 5mm PepMap Neo Trap Cartridge peptide trap column (C18 ...
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched to 0.5% trifluoracetic acid and spiked with Pierce Retention Time Calibrant (PRTC) peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/µL ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto an Acclaim Pepmap C18 5 µm-particle-size trap column (0.1 × 20 mm, Thermo Scientific #164564-CMD) and separated over a nanoEase M/Z Peptide BEH C18 1.7 µm-particle-size analytical column (0.075 × 250 mm ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were loaded onto an Acclaim Pepmap C18 5 µm-particle-size trap column (0.1 × 20 mm, Thermo Scientific #164564-CMD) and separated over a nanoEase M/Z Peptide BEH C18 1.7 µm-particle-size analytical column (0.075 × 250 mm ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were collected by centrifugation and labelled using tandem mass tag (TMT) 11-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: 50 µg of the peptides resulting after tryptic digest as described above were subsequently labeled using TMT-10plex reagents (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Elution was performed in 30μl of wash buffer plus 5μl of 1mg/ml 3xFLAG peptide solution (Pierce™ 3x DYKDDDDK, Thermo Scientific, A36805). Samples were incubated on ice for 15 minutes ...