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Citations for Thermo Fisher :
4401 - 4450 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Peptides (100 ng) were separated using Acclaim PepMap C18 columns (2 µm, 100 Å, 75 mm, 15 cm; Thermo Fisher Scientific). The peptides were loaded in buffer A (5% acetonitrile and 0.1% formic acid ...
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bioRxiv - Microbiology 2024Quote: A volume of 6 µl phosphopeptides or 500 ng of peptides were directly loaded onto reversed-phase pre-column (Acclaim PepMap 100, Thermo Scientific) and eluted in backflush mode ...
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bioRxiv - Molecular Biology 2024Quote: ... that were spiked with 18 pmol shTMTpro-labeled AS192 triggers were fractionated using the Pierce high pH reversed phase peptide fractionation kit (Thermo Scientific). A total of eight fractions were generated ...
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bioRxiv - Molecular Biology 2024Quote: Fractionated peptides were resuspended in 0.05% formic acid and separated using an UltiMate 3000 RSLC nano-LC system (Thermo Fisher Scientific) equipped with an analytical column (Waters nanoEase HSS C18 T3 ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated on a capillary column (0.075 mm × 500 mm, Acclaim Pepmap 100, reverse phase C18, NanoViper, Thermo Fisher Scientific) following a gradient of 2-40% buffer B in 128 min (A = 0.1 % formic acid B = 0.1% formic acid ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, ThermoFisher, US) using a 180-min gradient ...
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bioRxiv - Cell Biology 2024Quote: ... Online detection of peptide ions was by electrospray ionisation (ESI) mass spectrometry MS/MS with an Orbitrap Elite MS (Thermo Scientific). Ionisation of LC eluent was performed by interfacing the Sharp Singularity emitters (Fossil Ion Tech ...
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bioRxiv - Cell Biology 2024Quote: ... (Roberta Noberini et al., 2021) Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientific), 25-cm long (inner diameter 75 µm ...
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bioRxiv - Immunology 2024Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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bioRxiv - Cell Biology 2024Quote: ... the digest peptides from DMSO and Chk2i conditions were respectively spiked with the synthetic lysine-labelled heavy phospho-peptide of (phos)TLC(Carbamidomethylation)GTPNYIAPEVLSK(+8Da) that was synthesized by Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated using an Acclaim PepMap RSLC analytical column (C18, 100 Å, 75 μm × 50 cm, Thermo Fisher Scientific, USA) and Acclaim PepMap trap column (75 μm × 2 cm ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Equal amounts of peptide fragments from each experimental group were then labelled utilizing the TMTpro 16plex Isobaric Label Reagent (A44520, Thermo Fisher) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The synthetic peptides YNGVFQECCQAEDKGACLLPK (STD) and YNGVFQECCQA EDKacGACLLPK (STD*Kac) were analyzed using an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) equipped with an EASY-nLC 1200 system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: LC-MS/MS analysis of peptide samples was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA) coupled to an EASY-nLC 1200 liquid chromatography (LC ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 300 µL of 0.1% trifluoroacetic acid and desalted using Pierce™ Peptide Desalting Spin Columns (ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatography of peptides prior to mass spectral analysis was accomplished using capillary emitter column (PepMap® C18, 3µM, 100Å, 150x0.075mm, Thermo Fisher Scientific). Protein annotations ...
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bioRxiv - Cell Biology 2023Quote: The processing of BioID raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer v2.3 (Thermo Fisher Scientific). Raw mass spectra were filtered ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were then separated with an Acclaim PepMap RSLC column (75 µm ξ 25 cm, C18 2 µm 100Å, Thermo Scientific) at 0.25 µL/min with a gradient of 4-31% solvent B (80% I ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of amino acids and unique peptides was measured using an ultrahigh-performance liquid chromatograph tandem triple quadrupole mass spectrometer (Vanquish UHPLC-TSQ Altis MS, ThermoFisher, USA). To confirm the peptide structure ...
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bioRxiv - Developmental Biology 2023Quote: ... The resultant peptides were analyzed on an Advance UHPLC system (ABRME1ichrom Bioscience) connected to a Q Exactive mass spectrometer (Thermo Fisher) processing the raw mass spectrum using Xcalibur (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... TFEB tryptic peptides were dried and reconstituted in LC-MS mobile phase A (2% acetonitrile, Thermo Scientific, cat. no.047138.M6), 98% MilliQ purified water ...
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bioRxiv - Cell Biology 2023Quote: MS analysis of TMT-labelled peptides was performed on a Q-exactive-HF mass spectrometer coupled with a Dionex Ultimate 3000RS (ThermoFisher Scientific). The detailed LC-MS protocol can be found in the supplemental materials ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were redissolved in 100mM TEAB buffer and equal amounts of peptides from each sample were labelled with TMT 10-plex Mass Tag labelling kit (#90110, ThermoFisher Scientific). After labelling ...
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bioRxiv - Cell Biology 2023Quote: The processing of whole-cell protein quantitation raw LCMS data for peptide and protein identification and quantification was performed with Proteome Discoverer (PD v3.0 (Thermo Fisher Scientific) using a SPS MS3 reporter ion-based quantification workflow ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Systems Biology 2023Quote: Peptide digests of purified RSVF and antibodies were analyzed in DIA mode on a Thermo Scientific Lumos mass spectrometer (Thermo Fisher) equipped with a nanoelectrospray ion source and coupled to an Easy-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... internal diameter 100 μm) for desalting and a C18 analytical column (length 10 cm, internal diameter 75 μm) for peptide separation (Thermo Scientific). Peptides were eluted with a three-step 45 min gradient of 0.1% formic acid in ACN at a flow rate of 300 nl/min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2023Quote: ... The digested peptides were cleaned-up using the HiPPR detergent removal resin kit (PN 88305, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Fisher Scientific) fitted with a 100 μm × 2 cm PepMap100 C18 nano trap column and a 75 μm × 25cm ...
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bioRxiv - Immunology 2022Quote: Freshly isolated mouse splenocytes or LN cells were stimulated with an overlapping peptide pool of RBDWT (10 μg/mL) in the presence of Brefeldin A (Invitrogen, USA) for 5 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted for 1 h at room temperature with TMT10 (Thermo Scientific, #90110) or TMTpro 18-plex (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 500ng of each peptide fraction was analyzed by LC-MS on a Velos Orbitrap mass spectrometer (Thermo Scientific, Waltham MA). Online capillary LC ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately one µg or less for each SEC fraction was injected and the peptides were separated on an Acclaim PepMap RSLC column (150 mm × 75 µm, 2 µm particle size, ThermoFisher Scientific) at a flow rate of 300 nl/min ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides in each sample were labeled with one of the tags of TMTpro 18-plex labeling kit (A52045, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermofisher Scientific) using a 180 min gradient ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (Thermo Scientific), and separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was acidified with formic acid (5% final concentration) and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84868) according to Vinogradova and coworkers (Cell ...
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bioRxiv - Molecular Biology 2023Quote: ... peptides were desalted on StageTips (36) and analysed by nanoflow liquid chromatography on an EASY-nLC 1000 system (Thermo Fisher Scientific) coupled online to a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were subjected to tryptic digest and reductive alkylation using routine procedures and eluted peptides analysed by LC-MS/MS on a UltiMate 3000 RSLCnano System (Thermo Scientific) coupled to a LTQ OrbiTrap Velos Pro mass spectrometer (Thermo Scientific).
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bioRxiv - Systems Biology 2023Quote: ... Both the digested peptides from non-enriched and enriched samples were labeled with 16-plex TMT according to the manufacturer’s instructions (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pooled and dried to near completion under reduced pressure before resuspension in 1% formic acid and fractionation using a Pierce High pH Reversed Phase Peptide Fractionation Kit (ThermoFisher Scientific) with modified elution of 12 sequential fractions (10% ...
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bioRxiv - Microbiology 2023Quote: LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: Splenocytes were stimulated with the peptide pools described in the memory phenotypic assay and supplemented with anti-CD28 and anti-CD49d mAbs (ThermoFisher Scientific) both at 1.0 μg/ml or a tissue culture medium with 1% DMSO as a negative control ...
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bioRxiv - Cell Biology 2023Quote: The TMT-labelled peptide samples were analysed with an Easy-nLC1200 liquid chromatography system combined with Q Exactive HF mass spectrometer (Thermo Scientific) using a 136 min gradient ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of peptide samples were loaded onto a C18 reversed□phase trap column (100 μm × 2 cm, Thermo Fisher Scientific) equilibrated with 100% A phase ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting phosphopeptides and whole cell lysates (flow through) were fractionated using Pierce High pH Reverse-Phase Peptide Fractionation kit (Thermo Fisher). Samples were dried and reconstituted in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Plant Biology 2023Quote: ... and AtILR1 open reading frame sequences deleted of the 25-N-terminal signal peptide-encoding codons were amplified using Phusion Taq Polymerase (Thermo Fisher) prior to cloning into pETM11 plasmid in the BL21 (DE3 ...