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Citations for Thermo Fisher :
4451 - 4500 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The LC-MS/MS analyses of TMT-labelled peptides were performed on an Orbitrap Fusion Lumos Mass Spectrometer (Thermo Fisher Scientific) coupled with a Thermo Scientific Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... and AtILR1 open reading frame sequences deleted of the 25-N-terminal signal peptide-encoding codons were amplified using Phusion Taq Polymerase (Thermo Fisher) prior to cloning into pETM11 plasmid in the BL21 (DE3 ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG resin was washed with Trisbuffered saline (TBS) and eluted with 25 μL or 2 mg/mL 3X FLAG peptide (Fisher Scientific). Samples were analyzed by mass spectrometry as described below.
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bioRxiv - Biochemistry 2022Quote: ... The beads were bound to the magnet and the supernatant containing the peptides were extracted and dried in a SpeedVac (ThermoFisher Scientific). Samples were brought up in 50 μL of 100 mM TEAB and labeled with TMT10plex (LOT VG306772 ...
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bioRxiv - Biochemistry 2023Quote: ... Digested peptides were separated on an EASY-Spray column (25 cm × 75 μm ID, PepMap RSLC C18, 2 μm, Thermo Scientific) connected to an EASY-nLC 1000 nUPLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: All peptide samples were spiked in with iRT standard peptides (Biognosys AG, Schlieren, Switzerland) and applied to MS analysis using EasyLC nano HPLC (Thermo-Fisher Scientific), Vanquish Neo HPLC system (Thermo-Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18, 5 μm, 50 cm, 100 A° Easy nano spray column # ES903, Thermo Scientific) at a flow rate of 250 nl/minute flow rate by applying nonlinear gradient of solvent-B (80% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... A column for affinity purification was prepared by binding the peptide to a SulfoLink resin column using the SulfoLink Immobilization Kit (Thermo Fisher). Antibodies were affinity-purified from serum using this column.
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bioRxiv - Cell Biology 2022Quote: ... reversed phase liquid chromatographic separations of peptides were performed on a C18 EASY-spray and C18 trap columns connected to an Ultimate 3000 UPLC system (Thermo Scientific). Mass spectra were acquired on an Q Exactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Nanoscale liquid chromatography (LC) separation of tryptic peptides was performed on a Dionex Ultimate 3000 Rapid Separation LC system (Thermo Fisher). Peptides were loaded onto a 20 μL nanoViper sample loop (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were separated on a C18 Acclaim PepMap nanoLC column (75 μm x 50 cm nanoViper, PN 164942, Thermo Fisher Scientific) equilibrated with 4% solvent B (99.9% acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides were separated by means of nanoscale C18 reverse-phase Liquid Chromatography (LC) using an EASY-nLC II 1200 (Thermo Scientific) system directly coupled to a mass spectrometer (Orbitrap Fusion Lumos ...
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bioRxiv - Biochemistry 2023Quote: ... The malonylated synthetic peptide TVDGPSGKmaLWR* containing a stable isotope-labeled arginine residue (R* with 13C6 and 15N4) was obtained from Thermo Fisher Scientific at >90% chemical purity and >99% isotopic purity ...
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bioRxiv - Systems Biology 2023Quote: ... Peptides were enriched using a C18 precolumn and separated on a 50 cm analytical column (EASY-Spray Columns, Thermo Fisher Scientific) at 50°C using a 265 min gradient ranging from 0 to 40% acetonitrile/0.1% formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µg of non-phospho or phospho-CTD peptide (per condition) was conjugated to 10 µL (per condition) high-capacity streptavidin agarose beads (Cat#20357, Thermo Scientific) in peptide binding buffer (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Liquid chromatography (Ultimate 3000 nanoLC system) was used to fractionate the peptides online and then analyzed via an Exploris480 mass spectrometer (Thermo Fisher). MudPIT runs were carried out by 10µL sequential injections of 0 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm × 100 μm ID, 5 μm, 100 Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Physiology 2023Quote: LC-MS/SM analysis of digested peptides was performed on an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific) coupled to a nano-LC system (U3000-RSLC ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap, C18 5 µm, 100 µm x 2 cm, Thermo Scientific) connected to an analytical column (Acclaim PepMap 100 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were were analyzed with a nanoAcquity UPLC system (Waters) coupled with Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher), with a procedure published previously (20) ...
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bioRxiv - Cell Biology 2023Quote: ... Phospho-peptides were enriched in these samples using High-Select™ Fe-NTA Phosphopeptide Enrichment Kit according to manufacturer’s protocol (ThermoFisher Scientific). Phosphopeptides were resuspended in 133 mM HEPES (SIGMA ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were separated on an EASY-Spray PepMap RSLC C18 column (500 mm × 75 μm, 2 μm particle size, Thermo Scientific) over a 60-minute gradient of 2-35% acetonitrile in 5% dimethyl sulfoxide ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a nano-HPLC (Ultimate 3000, Dionex) with an inverse phase C18 Pepmap® column (Dionex, Thermo Fisher), following a gradient of 100% solution A (0.1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were first trapped on a C18 column (75-μm inner diameter × 2 cm; nanoViper Acclaim PepMap 100, Thermo Fisher Scientific) with buffer A (2:98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2022Quote: ... The flow through fraction was then fractionated into 13 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher).
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bioRxiv - Bioengineering 2023Quote: ... 2 µg of digested peptides were loaded into mass spectrometry system (Q Exactive Hybrid Quadrupole-Orbitrap MS, Thermo Fisher Scientific, USA). Hypersil gold C18 (100×2.1 mm ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of peptides for label-free LC/MS analyses were loaded to an Ultimate 3000 UHPLC/Q-Exactive system (Thermo Scientific) with an Acclaim PepMap RSLC 75 μm × 15 cm nanoViper column and run for 1 h.
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bioRxiv - Molecular Biology 2023Quote: ... A 2 μg aliquot of the peptide mixture were analysed using a LTQ-Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The precipitated pellets were processed as described in the proteomics section and peptides were cleaned up and stored on C18 HyperSEP cartridges at −80°C until use (Thermo Scientific).
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of amino acids and unique peptides was measured using an ultrahigh-performance liquid chromatograph tandem triple quadrupole mass spectrometer (Vanquish UHPLC-TSQ Altis MS, ThermoFisher, USA). To confirm the peptide structure ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pooled and desalted sample was fractionated using high pH reverse-phase chromatography on an XBridge peptide BEH column (130 Å, 3.5 μm, 2.1 × 150 mm, Waters) on an Ultimate 3000 HPLC system (Thermo Scientific/Dionex). Buffers A (10 mM ammonium formate in water ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: ... Peptides were then separated with an Acclaim PepMap RSLC column (75 µm ξ 25 cm, C18 2 µm 100Å, Thermo Scientific) at 0.25 µL/min with a gradient of 4-31% solvent B (80% I ...
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bioRxiv - Cancer Biology 2023Quote: Peptides were resuspended in 200 mM HEPES (pH 8.5) and a 100µg aliquot of peptides was mixed with tandem mass tags (TMT, ThermoFisher Pierce, Rockford, IL) at a label to protein ratio of 2:1 ...
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bioRxiv - Biochemistry 2023Quote: ... Interaction of His-fused proteins with other purified proteins (e.g. GST-PKAC) or synthetic peptides was studied by pulldown using Ni-NTA affinity resin (HisPur, Thermo scientific # 88221). GST-fused proteins were pulled down on glutathione-Sepharose beads (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was then added to a new tube and the peptides were extracted and desalted using C18 desalting columns (Thermo Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... The resultant peptides were analyzed on an Advance UHPLC system (ABRME1ichrom Bioscience) connected to a Q Exactive mass spectrometer (Thermo Fisher) processing the raw mass spectrum using Xcalibur (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: LC MS/MS data was acquired using a Dionex UltiMate 3000 RSLCnano for peptide separation and analysed with an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Scientific) with an Acclaim PepMap RSLC analytical column (75 µm x 50 cm ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptides were then reconstituted in MS-grade 0.1% FA in water before determining their concentration using a Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific 23290) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: Digested peptides were run on a Q-Exactive HF mass spectrometer coupled to an EASY-nLC II 1200 chromatography system (Thermo Scientific). Samples were loaded on a 50 cm fused silica emitter that was packed in-house with ReproSIL-Pur C18-AQ ...
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bioRxiv - Cell Biology 2023Quote: ... and about 1 µg peptide was loaded onto a 2-cm trap column and separated by a 50-cm EASY-Spray column (Thermo Scientific) heated to 55°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). For this ...
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bioRxiv - Cell Biology 2023Quote: ... TFEB tryptic peptides were dried and reconstituted in LC-MS mobile phase A (2% acetonitrile, Thermo Scientific, cat. no.047138.M6), 98% MilliQ purified water ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were redissolved in 100mM TEAB buffer and equal amounts of peptides from each sample were labelled with TMT 10-plex Mass Tag labelling kit (#90110, ThermoFisher Scientific). After labelling ...
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bioRxiv - Cell Biology 2023Quote: MS analysis of TMT-labelled peptides was performed on a Q-exactive-HF mass spectrometer coupled with a Dionex Ultimate 3000RS (ThermoFisher Scientific). The detailed LC-MS protocol can be found in the supplemental materials ...
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bioRxiv - Cell Biology 2023Quote: ... and the resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Mixed peptides were re-dissolved in 300 µl 0.1% trifluoroacetic acid and fractionated according to the instructions of Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher, Cat# 84868). Samples were eluted by gradient acetonitrile of 10.0% ...