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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: J774a.1 cells, HUVECs, and MCF-7 cells were purchased from Cellcook (Guangzhou, China) and cultured in DMEM (Gibco, Life Technologies, UK) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and ibuprofen (CAS 15687-27-1, purity ≥ 99%), and potassium chloride (KCl, CAS 7447-40-7, purity ≥ 99.7%) were purchased from Thermo Fisher (Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... which was conjugated at a 7:1 molar ratio with the cleavable EZ-Link Sulfo-NHS-SS-Biotin (#A39258, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Detection of VNNV and TiLV was performed using the Promega GoTaq Probe 1-step RT-qPCR System and both were performed in the QuantStudio 7 (Applied Biosystems, UK) as follows ...
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bioRxiv - Immunology 2020Quote: ... 1 × 105 MCF-7 cells were transfected with appropriate plasmid combinations using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... membranes were blocked in 7% milk in 1xTBS and 0.01% Tween-20 and streptavidin-HRP immunostaining (1:5000, Invitrogen cat. #19534-050) was performed at room temperature for 1h in blocking solution ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated for 7 min at 37°C with proteinase K in PBST (1/2000 from a glycerol stock at 20mg/ml, Ambion, Cat#10259184), washed in PBST for 2×10 min at RT ...
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bioRxiv - Immunology 2024Quote: ... then collected and plated 10:1 with allogeneic DC-10s in 24-well plates at a concentration of 7 × 105 to 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco, Thermo Fisher Scientific). For CRISPRa experiments ...
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bioRxiv - Bioengineering 2024Quote: Cell count was measured on days 1 and 7 using a using Quant-iT™PicoGreen™dsDNA Assay Kit (ThermoFisher Scientific). The scaffolds were washed for 5 minutes in PBS and then frozen at -80⍰C ...
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bioRxiv - Physiology 2024Quote: ... The starvation assay consisted of placing 5-7 day-old female DTKR-RNAi flies and control flies into vials containing 1% aqueous agar (Fisher Scientific, USA) [48,73].
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixtures were heated from 10°C to 70°C for approximately 1 hr by a QuantStudio 7 Pro Real-Time PCR System (Thermo Fisher Scientific). Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156, 5 μg/mL puromycin, Life Technologies A11138-03, and 2 μg/mL doxycycline). On day three ...
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bioRxiv - Immunology 2021Quote: ... After 3 consecutive washes anti-rabbit Alexa Fluor-568 conjugated antibodies (1:500; Thermo Fisher Scientific) were applied for 2 h before counterstaining with DAPI.
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bioRxiv - Microbiology 2020Quote: ... Parasitemias were determined on day 3 by staining with SYBR Green 1 nucleic acid stain (Invitrogen) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... slices were further incubated with TO-PRO-3 Iodide (diluted in PBS, 1:2000, Life Technologies) at RT for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... then 500 μL volumes of blood were diluted in sterile PBS (1:3) (BR0014G, Thermofisher Scientific) and centrifuged at 500xg for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... at a ratio of 1:3 of DNA: PEI in 200 μl of opti-MEM (Invitrogen) (Wang ...
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bioRxiv - Neuroscience 2020Quote: ... or 3) Goat anti-rabbit conjugated with Alexa Fluor 647 (1:1000) (Life technologies, Carlsbad, CA) for 2 hours at room temperature protected from light ...
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bioRxiv - Cell Biology 2020Quote: ... 3-NP-induced cytotoxicity was measured by fluorescence using the die YOYO-1 (#Y3601, Life Technologies). Treated cells were placed in an Incucyte FLR imaging system and the YOYO-1 fluorescence was measured after several time points ...
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bioRxiv - Bioengineering 2022Quote: ... in a ratio of 1:3 with a supplement of H2O2 (30%, Certified ACS, Fisher Scientific) at 200 °C for 50 min in a microwave digestion system (Titan MPS ...
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bioRxiv - Microbiology 2021Quote: ... were mixed at a ratio of 1 µg DNA : 3 µLTransit-T293 in Opti-MEM (Gibco). For an experiment conducted in triplicate at a 48-well scale ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were allowed to adhere for 1-3 hours before media was changed to Neurobasal (Invitrogen) supplemented with 1x B27 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: RNA from 1-3 x 106 cells was extracted and purified with TRIzol reagent (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 2016) are presented in Table 1 using an Applied Quant Studio 3 (Applied Biosystems, CA, USA). For PKoV RNA and PAstV-1 RNA detection ...
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bioRxiv - Microbiology 2020Quote: O/N bacterial cultures were diluted to an OD600 of 0.15 in TSB with 1 µg/mL 1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC12) (Invitrogen). Cultures were grown to an OD600 of 1.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... and the nuclei were stained with TO-PRO®-3 1:1000 (Thermo Fisher Scientific, USA) for 10–20 min ...
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bioRxiv - Microbiology 2021Quote: ... at a 1:3 ratio (mg DNA/mg PEI) mixed in Opti-MEM (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... The sections were counterstained with TO-PRO™-3 (T3605, Invitrogen, OR, USA; dilution 1:1000) and examined using a confocal laser microscope (DMi8 ...
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control ...
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bioRxiv - Cell Biology 2022Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated and transfected with 1:3 ratio (RNA:Lipofectamine) using Lipofectamine RNAiMAX reagent (ThermoFisher Scientific) following manufacturer recommendations ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: The stock solutions for the GLVs (Z)-3-hexen-1-ol (Z3-HOL, 98%; Acros Organics) and (Z)-3-hexenyl acetate (Z3-HAC ...
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bioRxiv - Immunology 2024Quote: ... and a 3:1 CD3/CD28 mouse T-activator Dynabead to Treg ratio (Thermo Fisher Scientific). On day 3 of expansion ...
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bioRxiv - Cell Biology 2024Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Cell Biology 2023Quote: PASMCs were stained with Hoechst 33342 dye (3 μL/ml of 1:100 dilution; 62249; Invitrogen) for 15-30 min at 37° C to visualize apoptotic cells ...
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bioRxiv - Cell Biology 2023Quote: ... with PBS (3 times) and incubated with a secondary antibody (1:500, Molecular Probes AF488 #A11008) and Hoechst33342 (1:2000 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... 1 mM L-cysteine) with papain (3 U/mL) and DNAse I (150 U/μL, Invitrogen) for digestion during 25 min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 µL of template cDNA using a QuantStudio 3 real-tame PCR system (Applied Biosystems). PP2AA3 was used for normalization ...
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bioRxiv - Cancer Biology 2023Quote: ... 3% agarose gels were prepared with a 1:20000 dilution of SYBRSafe DNA gel stain (Invitrogen). Once complete the PCR products were loaded onto the gel and the electrophoresis was run on a Powerpac Electrophoresis system (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then washed and incubated with a 1:1000 dilution of To-Pro-3 (ThermoFisher) in PBS for nuclear staining and then mounted using VectaShield antifade mounting medium (Vector Labs ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% P/S at a cell density between 3 × 105 and 2.5 × 106 cells/ml (GIBCO). Cells were always handled and maintained under sterile conditions (tissue culture hood ...
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bioRxiv - Genetics 2023Quote: ... the nuclei were stained by applying 3 mL of 1:1000 diluted DAPI (Thermo Scientific, 62248) in 2×SSC in 4 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then mounted using culture medium supplemented with 1 μM TO-PRO-3 (Life Technologies) for nucleic acid staining ...
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bioRxiv - Microbiology 2023Quote: O/N bacterial cultures were diluted to an OD600 of 0.15 in TSB with 1 μg/mL 1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC12) (Invitrogen). Cultures were grown to an OD600 of 1.0 ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-occludin (1:100 in 3% BSA in TBS; Thermo Fisher Scientific, Cat# 71-1500) overnight at 4°C in a humidified atmosphere ...
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bioRxiv - Biochemistry 2024Quote: ... 1-3 nmol of mixed antigen was bound to pre-equilibrated Dynabeads MyOne Streptavidin C1 (ThermoFisher). The purified phages were mixed with the beads pre-loaded with a combination of hCNPase and mCNPase and incubated for 2 h at RT ...
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bioRxiv - Immunology 2022Quote: ... B and E (+ SAgs) for 2 h in serum-free AIM V medium (Gibco, #12055-091). Unpulsed Raji B cells were used as negative control (-SAgs) ...