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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... tissues were incubated for 1 h at room temperature with fluorescent secondary antibodies and BTX–A555 (B35451, Life Technologies) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: The following secondary antibodies were used: goat anti-mouse IgG (H+L) secondary antibody (Thermo Fisher, 31430, 1:10,000) and goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated with the following fluorescence-labeled secondary antibodies at room temperature for 1.5 h at a dilution of 1:500: Alexa Fluor 488-labeled donkey anti-rabbit IgG (A21206; Invitrogen), anti-rat IgG (A21208 ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 μg of total RNA was used for cDNA synthesis using Maxima H Minus Master Mix (Thermo Scientific) in a reaction volume of 5 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were incubated for 1 h with the secondary antibody (goat anti-mouse Alexa Fluor 488, Invitrogen, A11029) diluted at 1:1000 in the Blocking Buffer supplemented with Hoechst diluted at 1:10,000 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed and incubated at room temperature for 1 h with 2.5 µg/mL goat anti-mouse IgG Alexa-Fluor 488 secondary antibody (ThermoFisher), and 5 µg/mL Uvitex 2B (Polysciences Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1 h at room temperature in a PBS solution containing 0.2 µg/mL Hoechst 33342 (H3570, ThermoFisher) and 1 µg/mL Streptavidin-Alexa488 (016-540-084 ...
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bioRxiv - Cell Biology 2020Quote: ... were injected in the pancreas with 4 ml of collagenase solution (7 ml HBSS, 70 μL HEPES (10 μM) (Gibco), 16,5μL DNAse (10mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec) in a QuantStudioTM 7 Flex Real-Time PCR System (Life Technologies). Protein thermal melting curve were generated using the Protein Thermal Shift TM software ...
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bioRxiv - Physiology 2020Quote: ... and incubated in cell* dishes at 37° C for 4 – 7 hours before adding cell culture medium (RPMI 1640, -glucose +glutamine, Gibco), supplemented with penstrep (10,000 U/ml penicillin / 10,000 μg/ml streptomycin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml or Dynabeads Mouse T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Cell Biology 2020Quote: ... guide RNA plasmid and a plasmid expressing SpCas9 at a ratio of 2:1:1 (total 12ug) using Neon electroporator (Life Technologies) and a 30ms ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were transiently transfected with HA-Flag-GHRHR-Rluc8 or HA-Flag-SV1-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using Lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Physiology 2019Quote: ... was digested in Collagenase II and DNase 1 (600 U/ml Collagenase II; CLS-2 Worthington, 60 U/ml DNAse 1; Ambion, Warrington) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Microbiology 2019Quote: ... Neural maintenance medium base (50% DMEM-F-12 medium-GlutaMAX, 50% Neurobasal-A medium, 1 × N-2 supplement, 1 × B-27 supplement [Life Technologies]) supplemented with 1% DMSO (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... fixed and permeabilized specimens were stained for 1 hour at room temperature by adding PermQuench buffer containing 1 μg/mL Hoechst 33342 and 2 μg mL-1 CellMask™ Deep Red plasma membrane stain (H32721, Invitrogen). After staining ...
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bioRxiv - Neuroscience 2020Quote: ... with 5% DMSO for at least 2 nights at room temperature with the following reagents: (1) 1:20 AlexaFluor 594 phalloidin (ThermoFisher #A12381) (Figure 3F ...
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bioRxiv - Cell Biology 2021Quote: ... Cardiac cells were subsequently thawed in RPMI+B27 media (RPMI 1640 supplemented with 2% B-27 Supplement minus vitamin A, 1% GlutaMAX and 1% Penicillin/Streptomycin- All from ThermoFisher Scientific) with Rock inhibitor (Selleck Chemicals ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transiently transfected with HA-GLP-1R-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Immunology 2020Quote: ... monocytes were plated in low-serum media (1% FBS) for 1-2 hours in 12-well Nunc-coated plates (ThermoFisher Scientific) to promote adherence ...
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bioRxiv - Biochemistry 2020Quote: The cDNA coding for SARS CoV-2 Nsp1 1-180 Nsp2 1-19 8His was synthesised by GeneArt (Thermo Fisher Scientific) adding NcoI and NotI sites at the 5’ and 3’ ends ...
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bioRxiv - Plant Biology 2022Quote: ... To each sample 0.7 µL of reverse transcription mix (8:2:1:1 of Superscript IV 5X Buffer, 100 mM DTT, RNase Inhibitor, Superscript IV; ThermoFisher Scientific) was added ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Zoology 2019Quote: ... 10-20 ng (1 to 2 μL) of genomic DNA and 1 unit Taq DNA polymerase (Platinum® Taq DNA polymerase, Invitrogen). PCR thermocycling profile was ...
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bioRxiv - Cell Biology 2022Quote: ... 2 ml of 56°C warm gel solution (1 volume RPMI/20% FBS, 1 volume 2x HBSS, 2 volumes 2.5% agarose in water (ultrapure agarose, Invitrogen, #16500-100)) were cast per 35 mm dish and allowed to solidify ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was then incubated for 1 hr at RT with 2° anti-rabbit HRP antibody (1:10,000, ThermoFisher, Cat #31458). The membrane was washed 5 times 5 min in TBS-T prior to visualization with SuperSignal West Pico Chemiluminescent Substrate (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 1 × 1 cm of mouse skin encompassing the wound site was excised into 2 mL of RNAlater stabilization solution (Invitrogen, USA) and incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... A subset of sections was stained for calbindin+ cells (1⁰ rabbit anti-calbindin (Swant #CB38) 1:500 + 2⁰ anti-rabbit-633 (Invitrogen #A21071) 1:500) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated in the dark at room temperature for 2 hours with 1:1000 DAPI and 1:500 AlexaFluor secondary antibodies (Thermo Fisher) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2-hour incubation with secondary antibody (1:500 in 1% BSA in PBST; AF488 anti-rat, Invitrogen, A-11006) at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Biochemistry 2023Quote: The datasets of ULK1C (2:1:1) core and the ULK1C:PI3KC3-C1 mixture were recorded at a 300 kV Titan Krios microscope (Thermo Fisher Scientific) equipped with X-FEG and energy filter set to a width of 20 eV ...
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bioRxiv - Biochemistry 2023Quote: ... fresh P3 virus from γ-TuRC-GFP and γ-TuSC were mixed in a 1:1 ratio and used to infect 1.8-2 L of High five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 30 mL of the γ-TuRC-GFP and γ-TuSC virus mixture as above ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Neuroscience 2023Quote: ... were selected and processed as described above with primary antibody Rabbit anti-Cox-2 (1:1k, Cayman Chem, 160123) and secondary antibody AF555 donkey anti-rabbit (1:200, ThermoFisher, A31572).
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bioRxiv - Neuroscience 2024Quote: Rhod2-AM (Fig. 2 and Fig. S2) or Oregon Green 488 BAPTA-1-AM (OGB-1; other figures; all ThermoFisher Scientific) were bolus-loaded as described 55 with minor modifications ...
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bioRxiv - Cell Biology 2024Quote: ... were added at a dilution of 1:500 in blocking solution (Aligent Antibody Diluent #S202230-2) containing 1:100 Phalloidin 594 (ThermoFisher #A12381) to cells and incubated at 4 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were incubated with fluorophore- conjugated secondary antibody in 2% milk in PBS with 0.3% triton X-100 for 1 hour (1:200, Alexa 555, Invitrogen A-21424) then mounted with DAPI-containing Fluoromount medium (Southern Biotech 0100-20) ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.1 M PB and stained with Alexa Fluor (AF) 647-conjugated streptavidin (1:300 diluted from 2 mg ml−1 stock, Invitrogen S32357) overnight in blocking/permeabilising solution ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C (for P. miniata) or 20C (for P. regularis) in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Immunology 2020Quote: ... 7-Aminoactinomycin D (7-AAD) and SYTOX green were purchased from Thermo Fisher Scientific (Burlington ...