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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The in vitro enzymatic assay with the fluorogenic substrate ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, UbiQ) was assembled in a 384-well microplate (Thermo Scientific). Assays were performed in 20 μL reaction volumes and in triplicate ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Microbiology 2022Quote: ... with 250ng each of the 8 plasmids corresponding with each segment of viral genome (or 7 plasmids for mock) and 4 µl Lipofectamine 2000 transfection reagent (Thermo Fisher) per well ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 99 °C with shaking for 7 min and 25 μg of protein per sample was analysed with 4-12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl was loaded on to NuPAGE 4 to 12% Protein Gels with 7 μl of 4X NuPAGE LDS sample buffer as described by the manufacturer (Invitrogen: NP0322BOX) and run for 2 h at 100 V ...
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bioRxiv - Neuroscience 2023Quote: Third instar larval brains were dissected in phosphate buffered saline (PBS) and fixed with 4 % formaldehyde (FA) pH 7 (Thermo Scientific)/PBS for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Movies were collected on a Titan Krios G3i at spot size 7 with a Falcon 4 Direct Detector (Thermo Fisher Scientific) equipped with an energy filter set to 20 eV ...
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bioRxiv - Biophysics 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... The RNA samples (1 μg each) reverse transcribed with the maxima H minus First Strand cDNA Synthesis kit (Thermo Fisher) with oligo-dT primers in a final volume of 20 μL ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody with Alexa Fluor 594 (A27016) 1:500 and DAPI (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: ... and alexa 568-conjugated goat anti-rabbit IgG (H+L) antibody (no. A-11036, Thermo Fisher Scientific, 1:1,000 dilution).
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Genetics 2021Quote: ... RNA (0.5 – 1 µg) was reverse transcribed to cDNA using Maxima H minus First Strand cDNA synthesis kit (ThermoFisher Scientific) using oligo dT18 (250 ng) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed X3 with PBS and incubated in the dark for 60 minutes at RT with Alexa Fluor 594 goat anti-rabbit IgG (H+L) (1:500 dilution in blocking buffer) (Invitrogen). Coverslips were washed X3 with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were subsequently rinsed and incubated for 1 h at RT with the appropriate HorseRadish-Peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualization of the signal was conducted using Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then washed with PBS to eliminate dead cells and then incubated for 1 h in 200 μL of HBSS (#14025092, Gibco, ThermoFisher) containing 100 μM of Resazurin (#B21187 ...
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bioRxiv - Neuroscience 2021Quote: ... before applying the corresponding secondary fluorescent antibodies (in PBS + 0.1% Triton X-100 + 10% FBS for 1 h at RT in darkness; Invitrogen, USA). Images were taken with Zeiss LSM5 Exciter and processed with Zen 2009 (Zeiss Ltd. ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated in Odyssey Blocking Buffer containing HRP-conjugated anti-rabbit IgG (H+L) (1:2000, Life Technologies 31462) or anti-mouse IgG (H+L ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Hoechst 33342 (IF, vessel-on-a-chip and whole mount stain 1:50.000) was purchased from Molecular Probes (H-1399). Mouse monoclonal actin antibody for western blot (1:2500 ...
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bioRxiv - Bioengineering 2019Quote: ... Secondary antibodies included goat anti-rabbit IgG (H+L) alexa fluor 594 conjugate (1:200, Thermo Fisher Scientific, Waltham, MA). An Olympus Fluoview 1000 inverted confocal microscope was used to image samples with a 20x PlanFluor dry objective (0.5NA) ...
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bioRxiv - Microbiology 2019Quote: ... Channels were blocked with 1% (w/v) BSA in PBS for 1 h at room temperature then gently flushed with warm bicarbonate-free RPMI 1640 (Invitrogen). Mid-trophozoite stage cultures were diluted to 3% parasitemia and 1% haematocrit in bicarbonate-free RPMI 1640 and pulled through the channel at 100 µl/min for 10 min at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... while Gout Anti-Mouse IgG(H+L) and Alexa Fluor® 488 (1:100; Thermo Fisher Scientific K.K., Tokyo, Japan) were applied as secondary antibodies for 60 min at 20 °C ...
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bioRxiv - Bioengineering 2020Quote: ... The plate was in-cubated for 1 h at room temperature with Pierce Protein-Free Blocking Buffer (Thermo Fisher Scientific) and again washed with PBS three times ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). For σ28 purification ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 25 absorption units of the cell lysate were incubated (1 h, 25°C, shaking at 850 rpm) with 62.5 U MNase (Thermo Fisher), 18.75 U RNase R (Lucigen) ...
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bioRxiv - Bioengineering 2020Quote: ... Cultures were labeled with either goat anti-rabbit IgG (H + L) Alexa Fluor 555 antibody (1:100) (Thermo Fisher Scientific) or rabbit anti-mouse FITC antibody (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were starved of serum for 1 h prior to two minutes incubation with Trypsin-EDTA (ThermoFisher Scientific, #25300-054), quenched with serum-free medium and centrifuged for 5 min at 300 x g ...
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bioRxiv - Cancer Biology 2020Quote: ... rinsed and incubated with secondary antibody Alexa Fluor 594 Goat Anti-mouse immunoglobulin G (IgG) (H/L) antibody (1:1000, Invitrogen) for 60 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... before 2-h incubation at room temperature with a secondary antibodies conjugated to Alexa Fluor 488 made in chicken (1:500; Invitrogen) and Alexa 555 made in rabbit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... before 2-h incubation at room temperature with a secondary antibodies conjugated to Alexa Fluor 488 made in mouse (1:500; Invitrogen) and Alexa 555 made in rabbit (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... These were further incubated for 1.5 h in room temperature with AlexaFluor 488 donkey anti-rabbit IgG (1:250, A21206, Invitrogen, Canada) and/or AlexaFluor 555 Goat anti-Mouse IgG (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... dried peptide sample was resuspended in 200 mM HEPES (pH 8) and incubated for 1 h at room temperature with one of the TMT10-plex reagents (ThermoFisher) solubilized in 100% anhydrous ACN ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 h with fluorophore-conjugated secondary antibody anti-rabbit-IgG Alexa Fluor®488 (Invitrogen A32731) and ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were incubated with the specific antibody overnight at 4 °C after adjusting the buffer to a final concentration of 150 mM NaCl and 0.5% NP-40 and then incubated for 1 h with Protein A-Sepharose™ 4B (Invitrogen), washed extensively ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM MgCl2) at 55,000 × g for 1 h in a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (ThermoFisher Scientific). The TB40r mGFP-IE-FKBP BAC was kindly provided by E ...
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bioRxiv - Microbiology 2020Quote: ... for MLKL detection followed by treatment with 1:1000 chicken anti-rabbit IgG (H+L) cross-adsorbed secondary Alexa Fluor 594 antibody (ThermoFisher), and 1:1000 goat anti-rat IgG (H+L ...
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bioRxiv - Microbiology 2020Quote: ... was used at a concentration of 1:100 followed by treatment with 1:1000 goat anti-rat IgG (H+L) cross-adsorbed secondary Alexa Fluor 594 antibody (ThermoFisher) to detect PARP ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation for 1 h at room temperature with the appropriate highly cross-adsorbed secondary antibodies (Invitrogen, California, USA). Immunoreactivity was detected by the avidin-biotin immunoperoxidase method (Vectastain ABC kit ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were allowed to swell in Milli-Q H2O for 1 h at 37°C and coated in sulfosuccinimidyl6-(40 -azido-20 -nitrophenylamino) hexanoate (sulfo-SANPAH, ThermoFisher) and photoactivated with 365 nm UV light for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare) ...
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bioRxiv - Systems Biology 2021Quote: ... followed by three PBS washes and 1 h incubation with Alexa Fluor 488-conjugated anti-rabbit secondary antibody (A-11008, ThermoFisher) diluted in 3% BSA ...
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bioRxiv - Microbiology 2021Quote: ... fixed parasites were incubated with 5µg/mL Blood Group Antigen H (O) Type 1 monoclonal antibody (Invitrogen 13-9810-82), washed three times with 1x PBS and incubated with 1:100 α-mouse secondary antibody conjugated to Alexa 488 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Pacific Blue (1:250, Molecular Probes P31582), goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2020Quote: ... and the appropriate Alexa Fluor™ 488 Goat anti-Rabbit IgG (H+L) (Cat no. A11034, 1:500, ThermoFisher Scientific) conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Donkey anti-Mouse/Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody conjugated to Alexa 488 (Life Technologies, 1/2000) were used to detect the primary antibody staining.
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bioRxiv - Cell Biology 2022Quote: ... The NFAp were incubated with primary antibodies for 1 h followed by secondary antibodies conjugated with either Alexa488 or Alexa594 (Invitrogen). SlowFade Gold Antifade Mountant (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were washed 3x with PBS and probed with Alexa Fluor 680 Goat Anti Mouse and Goat anti-Rabbit IgG (H+L) Alexafluor Plus 800 secondary antibodies (1/2000, Invitrogen) in 1% casein/PBS and visualized using a LiCor Odyssey infrared imager.
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bioRxiv - Neuroscience 2022Quote: ... rinsing with PBS (1x 1 h, 2x 30 min) was followed by staining with Alexa Fluor-conjugated secondary antibodies (Invitrogen, Waltham ...