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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1:1000), Blebbistatin (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... which was then blocked with 3% non-fat dry milk in PBS at room temperature for 1 h and incubated with primary antibodies (rabbit anti-pS404 Tau, CST-20194S; rabbit anti-pS262 Tau, Invitrogen-44-750G ...
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bioRxiv - Bioengineering 2022Quote: ... These were incubated for 1.5 h at room temperature with AlexaFluor 488 donkey anti-rabbit IgG (1:250, A21206, Invitrogen, Canada) and/or AlexaFluor 555 Goat anti-Mouse IgG (1:250 ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed thrice with DPBS, incubated for 1 h with AlexaFluor (488, 555, or 647) -labeled secondary antibodies (Invitrogen) diluted in blocking solution (1:250-1:1000) ...
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bioRxiv - Cell Biology 2022Quote: hTERT RPE-1 cells were transfected with 20 nM (final concentration) of the respective siRNA for 48 h using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the peptides from each sample were reacted for 1 h with the a ppropriate TMT-NHS isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rabbit IgG (H+L) conjugated with Alexa Fluor 488 was from Molecular Probes (A11008, 1/400 dilution). Positive cells were counted from blinded images using the Cell Counter tool of Fiji.
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bioRxiv - Molecular Biology 2020Quote: ... in blocking solution for 1 h at room temperature followed by 3x PBS rinses and incubating with Hoechst 33342 (1µg/mL Molecular Probes) and anti-rabbit AlexaFluor 546 or 568 (1:1000 Molecular Probes ...
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bioRxiv - Microbiology 2020Quote: ... in TBST buffer with 0.5% BSA and goat α-mouse IgG (H+L) HRP conjugated secondary antibody (1:10,000, Invitrogen A28177) were used to detect His-tagged proteins on the blot ...
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bioRxiv - Microbiology 2021Quote: ... and HepG2 cells were collected 46 h after infection of CSPwt or CSPmut sporozoites and lysed by 1 mL Trizol (Invitrogen). 100μL liver homogenate or 1 mL of cell lysis were used for total RNA extraction using Trizol in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... A second incubation was performed for 1 h at room temperature with anti-mouse Alexa Fluor 568-conjugated (Thermo Scientific) 1:200 in 1% PBSA and then incubated for 5 min with Syto9 (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-mouse were incubated for 1 h at room temperature and visualized by enhanced chemiluminescence (ECL, Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated at room temperature for 1 h with the polyclonal specific anti-oligomer A11 antibody (Life Technologies) or the fibril-specific monoclonal antibody OC (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... coverslips were washed three times in PB 0,12 M and incubated with a goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A594 (1:1.000, Life Technologies) antibody diluted in the blocking solution for 2H at RT ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times in PB 0,12 M and incubated with a goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A594 (1:1.000, Life Technologies) diluted in blocking solution for 2H ...
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A chlorzoxazone-baclofen combination improves cerebellar impairment in spinocerebellar ataxia type 1bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies used were as follows: Alexa Fluor 488 goat anti-mouse IgG (H+L) (1:200; Invitrogen, Cat. #A11001), Alexa Fluor 488 goat anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2% Triton X-100 at RT for 1 h, and then blocked with an anti-dsRNA antibody (Scicons, 10010200) and a fluorescent secondary antibody (Invitrogen). Images were captured with the CellInsight CX7 High-Content Screening (HCS ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated overnight at 4°C with antibodies against claudin-4 diluted 1:500 in 0.2% BSA followed by a 1 h incubation with Alexa Fluor 488-conjugated goat anti-rabbit (cat. no. A-11017; Invitrogen) diluted 1:500 and were finally mounted on microscope slides using Vectashield mounting medium (Maravai Life Science ...
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bioRxiv - Developmental Biology 2019Quote: ... After 1 h ROCK-inhibitor was removed and was replaced with standard growth media supplemented with Pen-Strep (Life Technologies). Cells were stimulated with the following ligands or small molecules 12 h after seeding ...
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Peptide Arrays of Three Collections of Human Sera from Patients Infected with Mosquito-Borne VirusesbioRxiv - Microbiology 2019Quote: ... The arrays were then incubated for 1 h with goat anti-human IgG tagged with Alexa Fluor® 488 (Invitrogen) as secondary antibody ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were rinsed briefly in sterile water and allowed to dry for 1 h prior to mounting to slides with ProLong Gold Antifade Reagent (ThermoFisher). All immunofluorescence images were captured using a Nikon SIM-E & A1R confocal microscope with an SR Apo TIRF 100x/1.49 NA oil objective lens ...
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bioRxiv - Physiology 2019Quote: ... Secondary antibodies were used at 1:1000 and included: goat anti-mouse IgG (H+L) dylight 488 (ThermoFisher Scientific, 35505); alexa fluor 488 goat anti-rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... of THP-1 monocytes +/− ML-60218 exposure and THP-1 macrophages (72 h PMA) using the mirVana miRNA isolation kit according to instructions (AM1560, Invitrogen). Nuclear and cytoplasmic THP-1 fractions were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... The cells were left to fix for 1 h at room temperature before transferring 5 μl into 15 μl ProLongTM Gold Antifade mountant (ThermoFisher). For cell membrane staining ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS twice and incubated for at least 1 h with 20 µg/ml streptavidin (Thermo Fisher) in 1 % BSA/PBS at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with PBS twice and incubated for at least 1 h with 20 μg/ml streptavidin (Thermo Fisher) in 1 % BSA/PBS at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS and incubated for 1 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing the indicated inhibitors ...
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bioRxiv - Molecular Biology 2020Quote: ... After washed three times with 1XPBS and incubated with Alexa Fluor 488 Goat anti-rabbit lgG (H+L) secondary antibody (1:500, Invitrogen) for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... After washed three times with 1XPBS and incubated with Goat anti-rabbit lgG (H+L) secondary antibody (1:500, Invitrogen) for 1 hours ...
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bioRxiv - Immunology 2020Quote: ... Digested sera was probed with Rabbit anti-Human IgG-HRP H+L Secondary Antibody (ThermoFisher Scientific Catalog #A18903, 1:10,000), Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423 ...
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bioRxiv - Cell Biology 2019Quote: ... incubated for 1.5 h with goat-anti-mouse Alexa 488 and goat-anti-rat Alexa 594(both 1:500, Life technologies) and mounted with Vectashield medium ...
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bioRxiv - Immunology 2019Quote: ... Briefly, cDNA were synthetized in 10 μl (50°C 1 h, 72°C 10 min) using SuperScript III Enzyme (ThermoFisher). After RNase H treatment ...
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bioRxiv - Genomics 2021Quote: ... Kc cells were incubated with 0.2 mM EU for 1 h and RNA was extracted with Trizol (Thermo Fisher Scientific). Next ...
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bioRxiv - Genetics 2020Quote: ... followed by a 1:1000 dilution of HRP-conjugated goat anti-mouse IgG (H+L) secondary antibody (#62-6520, Invitrogen). Detection was performed with SuperSignal West Pico Chemiluminescent substrate (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated with primary antibody solutions for 1 h at room temperature followed by treatment with secondary antibodies for 30 min along with Hoechst 33342 (Invitrogen). The coverslips were mounted using Fluoromount G (SouthernBiotech) ...
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bioRxiv - Physiology 2021Quote: ... Sections were then washed in PBS and incubated for 1 h in room temperature with donkey anti-rabbit Alexa-fluo (488) (Invitrogen) for KCC2 diluted 1:500 in blocking buffer ...
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bioRxiv - Physiology 2021Quote: ... Plates were incubated for 1 h at 37°C with 5% CO2 and were then overlaid with 1X MEM (Gibco) containing 2% FBS and 0.6% Avicel for 2 days ...
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bioRxiv - Immunology 2021Quote: ... slides were washed in PBS and then incubated for 1 h at room temperature with secondary antibodies including Goat Anti-Mouse IgG1 Alexa Fluor 488 conjugate (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Channel slides were blocked with 1% BSA/1xPBS for 1 h at room temperature prior to gentle washes with 37°C bicarbonate-free RPMI 1640 (Invitrogen). Mature infected cultures (3% parasitemia and 1% hematocrit ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated using 1 µg RNA and the Thermo Scientific RevertAir H Minus reverse transcriptase (Fisher Scientific UK: 11541515) supplemented with RNasin (Promega:N251S) ...
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bioRxiv - Microbiology 2021Quote: ... The blot was incubated for 1 h at room temperature followed by incubation with the mouse anti-V5 primary antibody (R960-25, Invitrogen) for 1 h rocking at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was then recovered and incubated for 1 additional h at 55 °C in the presence of 0.25 mg/ml proteinase K (Thermo Fisher). The eluate was finally purified with SPRI AMPure XP beads (Backman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... dilutions of IgG were co-incubated with the virus (1000-2000 TCID50) for 1 h at 37°C prior to addition of VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies (1:1000) used were goat anti-mouse IgG (H+L) Alexa Fluor 594 (Thermo Fisher Scientific, A11005), goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... the oocytes were incubated with the FITC-conjugated goat anti-mouse IgG (H + L) secondary antibodies (1:300, Molecular Probes) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibody: goat-derived anti-Rat IgG (H+L) conjugated to Alexa Fluor 488 (AF488) (dilution 1:250; Thermo Fisher). Samples were incubated at room temperature for 1h in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... cells were stained for 2 h at RT with a primary antibody followed by a secondary antibody staining for 1 h at RT (AlexaFlour-488-conjugated goat anti-mouse antibody (Invitrogen). Cells were imaged on Leica Dmi8 microscope ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with RSL3 and Ferrostatin-1 or Turofexorate/Fexaramine for 2.5 h before they were harvested using 0.05% Trypsin-EDTA (Thermo Fisher Scientific). Cells were counted and cell number was adjusted to the sample with the lowest cell count ...