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Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 1-2 μg of total RNA treated with DNase I (Thermo Fisher Scientific) was reverse transcribed using either the High-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... 1% (v/v) Hepes buffer and 0.05 M of 2-Mercaptoethanol (all Gibco) supplemented with 30% conditioned medium from mouse macrophage colony stimulating factor (M-CSF)-producing L929 cells ...
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bioRxiv - Evolutionary Biology 2019Quote: For RNA extraction the samples were homogenized in 1-2 ml TriZol (Thermofisher) using a Polytron PT Homogenizer (Kinematica) ...
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bioRxiv - Cell Biology 2020Quote: ... Eps8L2 siRNA #1 and Eps8L2 siRNA #2 contain Silencer Select modifications (Life Technologies), Ctdnep1 siRNA #2 and Nesprin2G siRNA were from Genecust Europe ...
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bioRxiv - Cell Biology 2020Quote: E14 mouse embryonic stem cells (Figures 1 & 2) were cultured in GMEM (Gibco) supplemented with 15% FCS ...
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bioRxiv - Immunology 2019Quote: ... Round 2 PCR amplicons were visualized using precast 1% agarose E-gels (Invitrogen), purified with the AMPure XP magnetic bead purification system (Agencourt) ...
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bioRxiv - Immunology 2021Quote: ... containing 2 mM EDTA and 1% L-Glutamine (Thermo Fisher Scientific, Waltham, MA). Erythrocytes were removed by incubating with red blood cell lysis buffer (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated corneas were fixed (2% PFA, 1 hour) and stained (Click-iT, Invitrogen) according to the manufacturer’s instructions followed by whole mount staining protocol (described above ...
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bioRxiv - Molecular Biology 2021Quote: ... at a 2:1 Lipofectamine/DNA ratio in OptiMEM (31985070, Thermo Fisher Scientific). Neuronal differentiation of embryonic stem cells was performed as previously described (27) ...
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bioRxiv - Immunology 2022Quote: ... SOLAμ horseradish peroxidase (HRP) 2 mg/1 ml 96-well plate (Thermo Scientific) was used to concentrate the peptides (according to the manufacturer’s instructions) ...
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bioRxiv - Cell Biology 2022Quote: ... SMPCs were switched to N2 media (containing 1% N-2 supplement (Thermo Fisher), 1% Insulin Transferrin Selenium ...
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bioRxiv - Cell Biology 2022Quote: ... 2° antibodies used: Alexa fluor 488 goat anti-rabbit 1:250 (Thermo Fisher Scientific Cat# A-11034 ...
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bioRxiv - Microbiology 2022Quote: ... and 2% agar (Agar bacteriological no. 1, Oxoid, Thermo Fisher Scientific, Waltham, USA). All strains were incubated aerobically ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated with 40,6-diamidino-2-phenylindole solution (DAPI, 1:10,000, Invitrogen) for 10 min and mounted in Mowiol medium ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Neuroscience 2022Quote: ... washed with PBST (2×10 min) and DAPI-mixed (1:10000, Thermo Scientific) PBS solution (1×20 min) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with 1-2 μM MitoSOX™ Mitochondrial Superoxide Indicator (Invitrogen) for 15 min at 37°C prior to harvesting ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were pulsed with 1 mM 5-Ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 3 hr ...
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bioRxiv - Neuroscience 2024Quote: ... calbindin (1⁰ rabbit anti-calbindin (Swant #CB38) + 2⁰ anti-rabbit-633 (Invitrogen #A21071)) ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclei were stained with DAPI (40, 6-diamidino-2-phenylindole; 1:5000, Invitrogen). The slides were mounted with a mounting medium (S3023 ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 X amphotericin and 0.8 μl/ml gentamycin) plus 2 mM EDTA (Invitrogen), followed by 2 washes with DPBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer containing 4ʹ,6-diamidino-2-phenylindole (DAPI, 1:1000, Invitrogen). Sections were embedded in fructose–glycerol clearing solution Image analysis was performed using ImageJ and Imaris software.
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bioRxiv - Pathology 2023Quote: ... Immunofluorescence staining of claudin-2 (1:100, Invitrogen Cat# 32-5600, RRID: AB_2533085) was performed on formalin fixed paraffin embedded human pancreas tissue and mouse pancreas organoid sections ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% phosphatase inhibitor cocktail 2 (1862495, Thermo Fisher Scientific, Waltham, MA, USA). Lysates of (20-30 μg of protein ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were injected with 2 nl of 1 mg/ml poly(I:C) (Invitrogen) or PBS as a vehicle control into the optic tectal ventricle by microinjection ...
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bioRxiv - Immunology 2022Quote: Expi-293F cells were purchased from Thermo Fisher and maintained in Freestyle293/Expi-293 media (2:1, v/v, Thermo Fisher) in polycarbonate shaking flasks (Triforest Labware) ...
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bioRxiv - Cell Biology 2023Quote: ... with sodium pyruvate (1 mM) and L-glutamine (2 mM) (Gibco, Waltham, MA), and without glucose at pH 7.4 at 37 °C) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% (v/v) HI-FBS and N-2 supplement (Gibco™). ATM knockout (KO ...
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bioRxiv - Cancer Biology 2023Quote: 1-2 × 105 cells were stained with a live/dead dye (ThermoFisher; L23102) in PBS for 10 min on ice in the dark ...
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bioRxiv - Immunology 2023Quote: ... In some experiments, αIFNγ neutralizing antibody (clone XMG.1, 2 μg/mL) (Thermofisher) was added to a cell culture ...
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bioRxiv - Genetics 2023Quote: ... Crosslinking was performed in 1 mL of PBS/1.4% formaldehyde/2% acrylamide (ThermoFisher) for 5 h at 37 °C in a wet incubator ...
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bioRxiv - Cell Biology 2023Quote: ... containing 2-mM L-glutamine supplemented with 1% nonessential amino acids (Gibco™), 1 mM sodium-pyruvate (GibcoTM) ...
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bioRxiv - Immunology 2023Quote: ... the pellet was washed 2 times with 1 ml 1x PBS (Gibco / 10010023). After it was centrifuged at 3000 rpm for 2 minutes and the supernatant was discarded ...
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bioRxiv - Physiology 2023Quote: ... Nuclei counterstaining was performed with Hoechst 33342 (2 μg.mL-1; Invitrogen Molecular Probes) during secondary antibody incubation ...
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bioRxiv - Physiology 2023Quote: ... Nuclei counterstaining was performed with Hoechst 33342 (2 μg.mL-1; Invitrogen Molecular Probes) during secondary antibody incubation ...
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bioRxiv - Bioengineering 2023Quote: ... SV-HUV-1 and HK-2 were cultured in DMEM/F-12 (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: siRNA (Dharmacon – Table 2) and Dharmafect 1 (Horizon) were combined in OptiMEM (Gibco) to achieve a final concentration of 20 nM and 0.1% respectively ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 µg plasmid DNA were added to 50 µl OptiMEM (Thermo Fisher) and another 50 µl PEI mix (OptiMEM with double the amount of PEI than DNA ...
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bioRxiv - Bioengineering 2023Quote: ... with 1-part 2× low glucose DMEM culture medium (Thermo Fisher Scientific, Australia). Hypoxic hydrogels were prepared in similar way except sodium sulfite or OxyraseTM was first added to the DMEM culture medium ...
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bioRxiv - Plant Biology 2023Quote: ... alpha Tubulin monoclonal primary antibody (YL1/2) (1:10,000; Thermo Scientific MA1-80017) and goat anti-rat IgG secondary antibody (1:10,000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 594 F(ab’)2 goat anti-mouse 1:2000 (Invitrogen A11020), Alexa Fluor 594 F(ab’)2 goat anti-rabbit 1:2000 (Invitrogen A11072).
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bioRxiv - Microbiology 2024Quote: ... mouse monoclonal anti-nucleocapsid (Thermo Fisher, MA5-29981, 1:500, 2 µg/mL), rabbit polyclonal anti-nsp3 (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... and SARS-CoV-2 nucleocapsid protein (N, clone E16C, Fisher Scientific, 1:25) were diluted in blocking solution and then added and incubated overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 594 F(ab’)2 goat anti-rabbit 1:2000 (Invitrogen A11072).
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bioRxiv - Cell Biology 2024Quote: ... Claudin 2 (Cat. No. 51-6100, 1:100, Thermo Scientific, Waltham, MA, USA). Next day ...
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bioRxiv - Immunology 2024Quote: ... 1 µg/mL anti-murine IL-2 (Cat# 14-7022 Invitrogen Carlsbad, CA), 20 ng/mL murine IL-6 (Cat# 216-16 Peprotech Cranbury ...
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bioRxiv - Immunology 2019Quote: ... MDSCs and monocytes were incubated at a concentration of 1-7×105 cells/well in FluoroBrite Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Waltham, MA) supplemented with 10% human serum ...
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bioRxiv - Cell Biology 2021Quote: ... Cells (1 × 107) from passage 5-7 were used for transplantation after staining with carboxyfluorescein succinimidyl ester (CSFE) (Invitrogen, Carlsbad, California, USA) on the day of the transplantation ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then stained intracellularly with guinea pig anti-NS6/7 (kind gift from Kim Green) and goat anti-guinea pig Alexa Fluor 647 (1:500, Life Technologies, #A21450). Cells were then washed twice with PWB and then resuspended in FACS buffer.
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bioRxiv - Cell Biology 2022Quote: J774a.1 cells, HUVECs, and MCF-7 cells were purchased from Cellcook (Guangzhou, China) and cultured in DMEM (Gibco, Life Technologies, UK) supplemented with 10% fetal bovine serum (FBS) ...