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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Plasma from LPS-treated mice were diluted 1:10 with Hanks’ Balanced Salt Solution (no Ca+2/Mg+2; Gibco) in 96-well black MaxiSorp™ plate ...
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bioRxiv - Neuroscience 2024Quote: ... and EGFP with 2:2:1 (total 0.5 µg / coverslip) using the Lipofectamine 3000 transfection reagent kit (Thermo Fisher Scientific). Transfected cells were identified by EGFP signals ...
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bioRxiv - Immunology 2024Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30°C for 18 hours at 200rpm ...
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bioRxiv - Genetics 2024Quote: Metabolites from LB broth were extracted via the addition of ice cold 2:2:1 (v/v) mixture of acetonitrile:methanol:water (#A456, #A955, #W6, respectively; Thermo Fisher Scientific) at a ratio of 30 µl sample per mL of extraction solvent ...
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bioRxiv - Biophysics 2024Quote: ... were seeded in a 6-well culture plate at a concentration of 2 x 105 cells ml-1 (2 ml per well in DMEM (Nissui) supplemented with 5 % fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Microbiology 2019Quote: ... with different spectral signatures selected for the detection of PfHRP2 and PAN-pLDH were washed with distilled water and activated with Sulfo-NHS (N-hydroxysulfosuccinimide) and EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (Pierce, Thermo Fisher Scientific Inc., Rockford, IL), both at 50 mg/mL ...
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bioRxiv - Microbiology 2020Quote: The plasmid SV-Psi--Env--MLV and L-LUC-SN were co-transfected with or without an envelope glycoprotein plasmid (pHCMV-VSV-G/pSARS-CoV-1/pSARS-CoV-2/pHIV-1 LAI gp160) into HEK293FT cells using Lipofectamine™ 3000 (ThermoFisher, US). Cell supernatants containing viruses were collected after 2 days of transfection ...
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bioRxiv - Genomics 2020Quote: ... or No Call (numerical representations 0, 1, 2, −1, respectively) using the BRLMM-P algorithm for 493,290 SNPs [25] (Affymetrix Power Tools (APT) Release 1.16.0) ...
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bioRxiv - Genomics 2020Quote: ... around 100 mg of liquid nitrogen frozen samples were ground with metal beads (2.5 mm diameter) in a Retschmill apparatus (24 hertz for 2×1 min) before re-suspending in 1 mL Trizol reagent (Thermo Fisher Scientific) and incubating at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Triton X-100 in 200 μL of PBS for 5 min and subsequently incubated in 100 μL of click reaction buffer (1× Click-iT cell reaction buffer [Thermo Fisher Scientific], 2 mM CuSO4, 1× Click-iT cell buffer additive [Thermo Fisher Scientific] ...
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bioRxiv - Biochemistry 2021Quote: ... equal numbers of THP-1 monocytes (2*105 ml−1) were seeded in RPMI 1640 without Lysine and Arginine (Thermo Fisher Scientific), supplemented either with natural amino acids (L-Lysine ...
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bioRxiv - Cancer Biology 2021Quote: Single live cell suspensions were washed with 1% FCS in PBS and resuspended in flow cytometry buffer (2% FCS and 1 mM ethylenediaminetetraacetic acid (EDTA; Thermo Fisher #AM9260G) in PBS).
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bioRxiv - Developmental Biology 2021Quote: ... The colonies were lifted with a 1:1 Dispase/Collagenase enzyme mix (1mg/ml; Gibco; 17105-041 and 2 mg/ml; Gibco; 17104-019). and resuspended in 10 ml of Phase 1 medium (1:1 IMDM (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... iAstrocyte differentiation was initiated by switching the medium to astrocyte medium (DMEM/F12+GlutaMAX, 1% N-2 supplement, 1% fetal bovine serum, all from Thermo Fisher Scientific) as previously described [81] and continued for at least 60 days ...
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bioRxiv - Microbiology 2022Quote: ... Human neuronal BE(2)-M17 cells (ATCC® CRL-2267) were maintained in a 1:1 ratio of F12 Hams and OptiMEM (GIBCO, USA) media supplemented with 10% horse serum (GIBCO ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with TBS for 10 min and then then nuclei were counterstained by incubation with diamidino-2-phenylindole dye (DAPI) (1:30,000 in PBS, from a 1 mg/ml stock solution, Thermo Fisher Scientific #62248) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... Accutase was removed and the cells were then resuspended in DMEM with 2% B-27-supplemented 1% FBS and 1% PSF (Thermo Fisher Scientific) and plated on PDL/Laminin-2020-coated culture plates.
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bioRxiv - Cancer Biology 2024Quote: Neuroblastoma BE(2)-C) cells were cultured in DME/F-12 1:1 (HyClone SH30023) supplemented with 10% Fetal Bovine Serum (Fisher Scientific FB12999102) and 1% Penicillin-Streptomycin (Lonza 17602E) ...
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bioRxiv - Cell Biology 2023Quote: ... and the nucleus was stained with diamidino-2-phenylindole dye (DAPI) (1:30,000 in PBS, from 1 mg/ml stock solution (Thermo Fisher Scientific #62248)) ...
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bioRxiv - Microbiology 2023Quote: ... cells with either MX2-T2A-TurboID or MX2-MUTT2A-TurboID lentivirus and selecting with 2 µg ml−1 puromycin (#ant-pr-1, Thermo Fisher Scientific) for 7 days ...
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bioRxiv - Microbiology 2023Quote: Pseudotyped viruses were titrated by serially diluting filtered pseudotypes with fresh DMEM starting from a 1:2 to a final 1:512 dilution using white 96-well flat bottom plates (ThermoFisher, Woolwich, UK). Then appropriate target cells were added (Table 1 ...
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bioRxiv - Genetics 2023Quote: ... The solution was then exchanged with primary antibody diluted in a staining solution (1% DMSO, 0.5% Triton X-100, and 2% NDS in PBS): Sox2 (1:200; Invitrogen, 14-9811-82) or AF647-conjugated β-catenin (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... and fibroblasts were cultured for 2 days in DMEM/F12 (1/1) supplemented with 10 % fetal bovine serum (Fetal Clone II; Hyclone, Thermo Scientific, France) and 1% Penicillin/Streptomycin (Sigma) ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 Nucleocapsid antibody (produced in mouse) as a primary antibody was procured from (MA5-35943, Invitrogen, 1:1000-1:10,000), Rabbit phospho-p38 MAPK and total p38 MAPK Antibody were procured from (9211S and 9212S ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice with PBS (1X) and incubated for 2 hours at 37°C with secondary detection antibodies (1:500) and phalloidin (1: 3000) (Invitrogen, Waltham, MA) for cytoskeleton staining ...
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bioRxiv - Microbiology 2023Quote: ... mosquito bodies and legs were collected and placed in homogenizer tubes with a homogenizer bead and 500μl mosquito homogenization media (DMEM containing 2% FBS, 1% PenStrep, and 1% Fungizone (Gibco, Grand Island, NY)) ...
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bioRxiv - Biochemistry 2024Quote: ... Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg) was injected onto a Dionex UltiMate 3000 RSLCnano LC system (Thermo Fisher Scientific) with an additional RSLCnano pump run in online two-dimensional active modulation mode coupled to a Thermo Fusion Orbitrap mass spectrometer.
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bioRxiv - Cell Biology 2024Quote: ... Then CB were diluted 1:1 (v/v) into a dilution buffer (1mM EDTA in PBS supplemented by 2% FBS (Thermo Fisher, #10099141)) and undelayed with density gradient medium LymphoPrep (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were labeled with 1 μg/mL mAb 13-2 (rOmpB) (53) and 1 μg/mL rabbit polyclonal αTGN46 (Invitrogen, PA5-23068) in PBS overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ml of collagenase mix digestion solution (collagenase II + IV mixed at 1:1 ratio at 2 mg/ml -Thermo Fisher Scientific) was added to the tissue and incubated horizontally for 20 min at 37°C in a shaking incubator ...
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: 10 uL of either Caspase-3 or −7 library glycerol stocks was used to inoculate 5 mL of auto-induction media (Invitrogen Magic Media) and allowed to incubate and express for 18 hours at 30 °C ...
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bioRxiv - Immunology 2021Quote: The enumeration of apoptotic cells was performed using the CellEventTM Caspase-3/7 Green Flow Cytometry Assay Kit (catalogue #: C10427; Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Human breast carcinoma cell lines (SK-BR-3, MCF-7, MDA-MB-231, T-47D) were cultured in GIBCO® RPMI 1640 (Life technologies) medium supplemented with 10% FBS (Life technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were passaged on day 3 to maintain sub-confluent culture conditions and analyzed by flow cytometry on day 7 using an Attune NxT (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... Genital ridges from 1-2 litters (7-8 embryos per litter) were dissected out and digested at 37°C for 3 min using TrypLE Express (Thermo Fisher Scientific). For E9.5 and E10.5 stages ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was extracted from 40 pairs of adult testes per sample per condition (3 to 7 days post-eclosion) using the PureLink RNA Mini Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer containing 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies). A BD FACSAria III Cell Sorter (BD Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... Cell nuclei were counter stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes) prior to mounting onto glass slides and then cover slipped with ProLong Gold (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... re-suspended in PBS containing a viability marker (7-AAD – 7-Aminoactinomycin, Invitrogen – A1310), filtered again through 70-µm cell strainers and incubated on ice for 5 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... 7 and 7 fish respectively and dissociated in 0.25% Trypsin-EDTA (Gibco, ThermoFisher Scientific) and 8 mg/ml Collagenase from Clostridum histolyticum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 7 and 7 fish respectively and dissociated in 0.25% Trypsin-EDTA (Gibco, ThermoFisher Scientific) and 8 mg/ml Collagenase from Clostridum histolyticum (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were incubated overnight with Paired Box 7 (PAX 7) antibodies (Invitrogen, USA) in blocking solution at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were first loaded for 3 min onto the Acclaim PepMap 100 C18 trap column (75 µm x 2 cm, 3 µm material, Thermo Scientific) with 5 µL min-1 of 3.2% (v/v ...
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bioRxiv - Physiology 2019Quote: Total RNA samples were extracted from one thousand 4-7 day-old Culex female antennae with TRIzol reagent (Invitrogen, Carlsbad, CA). Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: About 80,000 MCF-7 cells were placed in each chamber of a 4-well chamber slide (Thermo Scientific, cat. no. 177399) containing 500 μl of Dulbecco’s Modified Eagle Medium (DMEM ...