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Citations for Thermo Fisher :
4301 - 4350 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 1% penicillin-streptomycin and 4 mM L-glutamine (all three from Gibco, Thermo Fisher Scientific). Cells were plated at a density of 120,000 per dish either on high precision #1.5 25 mm coverslips (Marienfeld ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were seeded in a 4-chamber glass bottom dish (D35C4-20-1-N, Invitrogen) for confocal imaging or in a 60 mm × 15 mm dish (705001 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1-20 µg of protein was loaded onto 4-12% NuPage BisTris gels (Invitrogen) for electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: ... and Alexa Fluor 647 (4 µg/mL, A21244, Invitrogen and 1:500, #4410, Cell Signaling). To stain for nucleus and cytoskeleton ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... PBS (pH 7.4) containing 2X ethidium homodimer-1 (4 μM; #L3224B, Thermo Fisher Scientific, USA) was added to the existing medium in each well at a 1:1 ratio ...
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bioRxiv - Microbiology 2024Quote: ... AF647-conjugated donkey anti-sheep IgG (Thermo Fisher, A-21448, 1:500, 4 µg/mL), CF568-conjugated donkey anti-goat IgG (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... AF647-conjugated donkey anti-rabbit IgG (Thermo Fisher, A-31573, 1:500, 4 µg/mL), AF647-conjugated donkey anti-sheep IgG (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 × 2 minutes and blocked with SeaBlock blocking buffer (ThermoFisher) for 15 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: The ability of the tissues to produce testosterone was assessed after 3 h of incubation with DMEM/F12 (Gibco) containing 0.1% BSA ...
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bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
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bioRxiv - Physiology 2019Quote: ... 10 μm thickness muscle cryosections were incubated with 5 μM of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH; Molecular Probes, Eugene) and allowed to dry overnight at room temperature in dark ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Liver-Chips were stained in the upper channel with 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA) (Thermo Fisher) to visualize bile canaliculi and MRP2 activity ...
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bioRxiv - Molecular Biology 2022Quote: ROS levels were detected using the chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) general oxidative stress indicator kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto a nitrocellulose membrane using iBlot 2 gel transfer device (7 min at 25 V, ThermoFisher). Membranes were incubated for 1 hour at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher), 200 μM L-ascorbic acid (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The reaction mixture was then transferred to 2 ml ZebaSpin MWCO 7 kD desalting column (Thermo Fisher Scientific, USA) to separate the labeled protein from the free dye ...
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bioRxiv - Microbiology 2024Quote: Neutrophils were resuspended in HBSS with Ca2+ and Mg2+ containing 10 μM 2’,7’- dichlorodihydrofluorescein diacetate (DCF) (Molecular Probes) to a final concentration of 1×107 cells/ml and gently agitated for 10 minutes at room temperature ...
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bioRxiv - Genetics 2021Quote: ... and incubated overnight at 4°C with mouse anti-H4K3me3 (Wako #305-34819 at 1:500 dilution in PBST) or rabbit anti-H3K4me2 (Invitrogen #710796 at 1:20,000) primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Cells are differentiated for 4 days in embryonic body (EB) differentiation medium (DMEM/F12 [Hyclone], 20% FBS, NEAA [1×, GIBCO], GlutaMAX [1×, GIBCO], 0.1% beta-Mercaptoethanol [GIBCO]). For mixed embryonic body (EB ...
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bioRxiv - Neuroscience 2022Quote: ... A 100 mM stock solution (in 0.2 M NaOH and 15% DMSO) of TCO*A-Lys was diluted 1:4 in 1 M HEPES (Thermo Fisher Scientific, cat. no. 15630056), and added to the medium at a final concentration of 250 μM ...
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bioRxiv - Genomics 2022Quote: ... at a bead-to-cell ratio of 1:1 in the presence of recombinant IL-4 (Fisher Scientific, #204-IL-010, 10 ng/ml) and anti-IFN-γ antibody (10 μg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... a stock solution of UAA (100 mM in 0.2 M NaOH containing 15% DMSO) was diluted 1:4 in 1 M HEPES (Thermo Fisher Scientific, cat. no. 15630080) and added to cells to a final concentration of 250 µM for TCOA-Lys and TCO4en/eq-Lys ...
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bioRxiv - Cell Biology 2022Quote: siRNA transfections were carried out using 20 pmol of siRNA (SI Table 1) targeting LTBP3 (siLtbp3-1-4) and lipofectamine RNAiMAX (Invitrogen, Waltham, MA; 13778-075) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were probed with respective primary antibodies overnight at 4°C: anti-ZO-1 (1:500, Invitrogen, San Diego, CA, USA, Cat# 61-7300), anti-VE-Cadherin (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... were subjected to overnight incubation at 4°C with a rabbit monoclonal anti-ADRA2A antibody (Thermo Fisher Scientific, Catalog #14266-1-AP, 1:200). Signal amplification was facilitated using the Dako envision + system-HRP labeled polymer anti-rabbit antibody (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa antibody65 (1 : 500 dilution) at 4°C overnight and secondary antibody (Alexa Fluor™ 647 chicken anti-rabbit IgG, Invitrogen; 1 : 500 dilution) at room temperature for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were then incubated overnight at 4 °C in rabbit anti-MYOC (1:50, custom) and rat anti-GFAP (Life Technologies, 130300, 1:500) in 5% NGS (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A, custom antibody from Thermo Fisher 1:1000). Secondary antibody staining was performed using an anti-rabbit antibody (Azure anti-rabbit NIR700 or Azure anti-rabbit NIR800 at 1:20000 dilution) ...
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bioRxiv - Molecular Biology 2021Quote: ... for 4 h at 55°C and DNA was purified using DNA clean-up kit (Thermo Fisher Scientific). DNA fragmentation range was assessed by electrophoresis.
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bioRxiv - Neuroscience 2021Quote: ... The brains were dissected and post-fixed for 72 h in fresh 4% PFA (Thermo Fisher Scientific #28906) before being embedded in low melting point agarose (Thermo Fisher Scientific #R0801 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 10 μM EdU for 4 h using the Click-iT EdU microplate Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 4 h end-to-end incubation with 300 μg /mL of Protein G magnetic beads (Thermofisher) in 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions (200 μL) were incubated for 4 h in 8-well Nunc Lab-Tek chambers (Thermo Scientific #155361). Brightfield and Cy3 fluorescence images were captured on a Nikon Ti Eclipse wide-field microscope ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated for 48 h at 4°C in secondary antibodies: goat Alexa Fluor-conjugated antibodies (Invitrogen) directed against rat (Alexa Fluor 647 ...
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bioRxiv - Microbiology 2020Quote: ... 4 and 24 hours after treatment and the plasma membrane was stained with FM 4-64 from Life Technologies (100 μg ml-1). Cells were observed with an epifluorescence Leica DM6000 B research microscope with a 100 x magnification.
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bioRxiv - Pathology 2023Quote: ... Sections were cut (4 µm) using a cryostat and labelled overnight at 4°C with COL6 antibody (1:500) (MA5-32412, Thermo Fisher Scientific, USA) after blocking nonspecific binding sites with 10% bovine serum albumin (A7906 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500 ...
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bioRxiv - Immunology 2022Quote: ... ∼50% of total lung was placed in a bead homogenizer tube with 1 mL of PBS with 2% FBS and 2% antibiotics/antimycotics (Gibco) and stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: ... The gland/crypt-containing supernatant was washed by centrifugation at 400 x g for 2 minutes and the glands/crypts re-suspended in 1-2 ml advanced DMEM/F12 (12634-010; Gibco) containing 1X B27 supplement minus vitamin A (12587-010 ...
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bioRxiv - Cancer Biology 2021Quote: ... were cultured in EBM-2 Basal Medium supplemented with EGM-2 MV Microvascular Endothelial Cell Growth Medium and 1% v/v Pen-Strep (ThermoFisher) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: The monoclonal antibodies SARS-CoV-1/SARS-CoV-2 Spike Protein S2 (1A9) and SARS-CoV-1/SARS-CoV-2 Nucleocapsid (6H3) were purchased from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... NPCs were plated on Geltrex in a 6 well plate in StemPro NSC SFM supplemented with 20ng/ml of EGF and bFGF for 2 days before switching to astrocyte differentiation medium consisting of DMEM with 1% N-2 supplement (Gibco), 1% GlutaMAX-I supplement (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... was diluted 1:1 with PBS (GE lifescience) with the addition of 2% fetal bovine serum (GE lifescience) and 2 mM EDTA (Ambion), added to lymphoprep tubes (Alare Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... at a concentration of 2 × 105 cells ml−1 (2 ml per dish hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% FBS (Gibco), glutamine ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM D-Glucose, 2 mM L-Glutamine, 1 mM Sodium Pyruvate, 140 mM KCl and 20 mM HEPES, Invitrogen) for longer imaging sessions ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Developmental Biology 2024Quote: ... in facility water (2-month-old fish) or an E3 solution supplemented with 0.2 mM 1-phenyl-2-thiourea (PTU; Acros Organics) (larvae) ...