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Citations for Thermo Fisher :
4001 - 4050 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... For CD26+ and Sca1+ fibroblasts dermis was separated from the skin of P2 or P3 mice in 1 mg/ml of dispase for 1 h at 37 °C and digested in 2.5 mg/ml of collagenase IV (Gibco) for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... either left untreated (DMSO control) or treated with 1 µM MG132 for 12 h using TRIzol reagent according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific Inc. ...
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bioRxiv - Neuroscience 2019Quote: ... followed by incubation with a goatanti-mouse IgG (H+L) poly-HRP secondary antibody (1:250 Invitrogen, MA, USA) for one hour at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The cells were finally stained with calcofluor white (5 μg/mL for 1 h at 37°C; Invitrogen, USA). Alternatively ...
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bioRxiv - Neuroscience 2020Quote: ... cells were permeabilized and blocked for 1 h at RT using 0.1% Triton X-100 (Thermo Fisher Scientific, 28313) and 4% donkey serum in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA samples (1 μg) were reverse-transcribed using RNase H-MMLV reverse transcriptase (Superscript II, Invitrogen, Carlsbad, California) and Oligo (dT)™ primers (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and Goat Anti-Rabbit IgG (H+L) Alexa Fluor 647 (Invitrogen, Thermo Fisher Scientific, A21245, 1:500 in PBS). Wheat germ agglutinin (WGA ...
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bioRxiv - Cell Biology 2019Quote: ... secondary antibodies coupled to horseradish peroxidase were added for 1 h at RT before ECL detection (Thermo Fisher Scientific). Immunoblots were quantified using ImageJ.
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies are horse-radish peroxidase-conjugated goat anti-mouse IgG (H+L) at 1:5000 (Thermo Scientific, 31430) and horse-radish peroxidase-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h before opsonization with human IgG or 200 µg/mL anti-BSA antibody (Invitrogen, Burlington, ON, Canada). RAW cells were pre-cooled to 4°C for 5 min prior to the cells being presented with targets at ratios of 20-400 rods per cell ...
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bioRxiv - Immunology 2021Quote: ... The staining was revealed using IgG (H&L) specific secondary antibody HRP-conjugated (1:500, Novex by Life Technologies) and DAB (3,3’-Diaminobenzidine ...
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bioRxiv - Cell Biology 2021Quote: ... samples were washed 3X with PBST at RT for 1 h and mounted with SlowFade Gold antifade reagent (ThermoFisher). Samples were analyzed using a Nikon inverted TE2000-E microscope equipped with phase contrast and epifluorescence ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor 546-conjugated goat polyclonal anti-mouse IgG (H+L) (1:500; A11030, Molecular Probes, Eugene, OR, USA); Alexa Fluor 546-conjugated goat polyclonal anti-rat IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were first pre-incubated for 1 h in glucose-free RPMI 1640 GlutaMAX-I medium (Life Technologies, Inc.), followed by incubation with Krebs-Ringer buffer (KRB ...
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Purkinje cardiomyocytes of the ventricular conduction system are highly diploid but not regenerativebioRxiv - Developmental Biology 2022Quote: ... goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody AlexaFluor 647 conjugate 1:500 (A21244, Thermo Fisher Scientific). Intestines were removed freshly and formed into Swiss rolls [18] and fixed overnight in 10% formalin ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthetized by reverse transcribing 1 μg of total RNA using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Cultures were washed and incubated for 3 h with appropriate secondary antibodies (1:1000, in PBS with 10 % NGS or NHS, 0.1 % Triton X-100; Invitrogen). TO-PRO® or DAPI (1:5000 in PBS for 10 min ...
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bioRxiv - Physiology 2024Quote: ... secondary antibodies were applied including Alexa flour 647 donkey anti-rabbit IgG (H+L) (1:200, A31573, Life technologies) and in 5 % of skim milk in PBS-T for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and Goat anti-Mouse IgG (H+L) Alexa Fluor 594-conjugated secondary Ab (1:200; Invitrogen, Cat# A11005, RRID:AB_2534073) were used ...
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bioRxiv - Neuroscience 2023Quote: ... and Goat anti-Rabbit IgG (H+L) Alexa Fluor 488-conjugated secondary Ab (1:200; Invitrogen, Cat# A11034, RRID:AB_2576217) were used ...
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bioRxiv - Physiology 2024Quote: ... Slides were washed with TBS and incubated for 1 h in the dark with AlexaFluor 555 or 647 (Invitrogen) secondary antibodies at 1:500 dilution and 2% normal donkey serum ...
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bioRxiv - Cancer Biology 2024Quote: Cells were assessed 96 h post incubation onset by the addition of 1/10 volume of alamarBlue (ThermoFisher Scientific) and a 1 h incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies and concentrations used were Alexa Fluor 488 goat anti-rabbit IgG (H+L) (Invitrogen, A11008, 1:500), and Alexa Fluor 594 goat anti-mouse IgG1 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and then with donkey anti-mouse IgG (H+L) secondary antibody (Alexa Fluor 647; Thermo Fisher; A31571; 1:1000). Immuno stained cells were analyzed on an FACScan Canto RUO flow cytometer (BD ...
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bioRxiv - Systems Biology 2023Quote: ... Gels were run at 200 V for approximately 1 h and incubated with SYBR Gold nucleic acid stain (Invitrogen), diluted 1:10,000 in TBE buffer (90 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis from 1 μg total RNA was carried out using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific) and oligo dT12–18 (Life Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... MPSF and NPSF were treated for 1 h with IM 0.5 μM or DMSO as vehicle in DMEM high glucose (#41965-039; Gibco). Mouse and NMR SV40-Ras skin fibroblasts were treated for 15 and 10 min with IM 2.5 μM ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor™ 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (1:250, Invitrogen, A31573). Alexa Fluor 647 Phalloidin (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... Signal was enhanced using a triple western blot protocol with biotin conjugated goat antirabbit IgG and Alexa Fluor 680 conjugated S-32358-streptavidin (1:100,000)35 and 31820-Goat anti-rabbit IgG (H+L) secondary antibody Biotin IgG (1:100,000) from Thermo Fisher Scientific were used.
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bioRxiv - Bioengineering 2023Quote: ... sections were incubated with Alexa Fluor 555-coniugated goat anti-mouse IgG (H+L; Thermo Fisher Scientific, 1:400) and Alexa Fluor 488-coniugated goat anti-rabbit (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... cells were first incubated at 37 °C for 1 h in buffer H1 (50% formamide (FA, Cat. # AM9342, ThermoFisher), 10% (w/v ...
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bioRxiv - Immunology 2023Quote: ... or horseradish peroxidase (HRP)-conjugated rabbit anti-guinea pig IgG (H+L) antibody (1:500; Invitrogen, Cat. PA1-28597) in blocking buffer for 1-1.5 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: ... and Goat anti-Mouse IgG (H+L) Alexa Fluor 488-conjugated secondary Ab (1:200; Invitrogen, Cat# A11001, RRID:AB_2534069) were used ...
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bioRxiv - Neuroscience 2023Quote: ... and Goat anti-Rabbit IgG (H+L) Alexa Fluor 594-conjugated secondary Ab (1:200, Invitrogen, Cat# A11012, RRID:AB_2534079) were used ...
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bioRxiv - Biophysics 2023Quote: ... after the washing step with secondary antibodies HRP-conjugate Goat anti-Mouse IgG (H+L) (1:5000, #89842, Invitrogen), HRP-conjugate Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 1 h with appropriate secondary antibodies conjugated with specified fluorophores (Thermo Scientific, West Palm Beach, FL), including Alexa Fluor 488-labeled donkey anti-mouse-IgG antibody ...
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bioRxiv - Genomics 2023Quote: ... or mouse anti-YAP1 H-9 (1:150, sc-271134) in a PBS solution containing donkey serum albumin and 0.1% Trizol (ThermoFisher). Cells were washed with PBS (3x for 15mins ...
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bioRxiv - Neuroscience 2023Quote: ... and Goat anti-Mouse IgG (H+L) Alexa Fluor 594-conjugated secondary Ab (1:200; Invitrogen, Cat# A11005, RRID:AB_2534073) were used.
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bioRxiv - Molecular Biology 2023Quote: ... and blocked for 1 h at RT in PBST containing 10% of fetal bovine serum (FBS) (Thermo Fisher Scientific). Cells were subjected to immunostaining overnight at 4°C with primary antibodies (Table S3 ...
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bioRxiv - Neuroscience 2023Quote: ... or Alexa Fluor 488 goat anti-mouse IgG H&L (1:400; Thermo Fisher Scientific, Catalog no. A-11001)—for 2 h followed by staining in DAPI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... with Goat Anti-Mouse - IgG (H+L) Highly cross-adsorbed secondary antibody - Alexa Fluor647 (1:1000; ThermoFisher, A-21236), DAPI (1:1000 of 20 mg/mL stock ...
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bioRxiv - Genetics 2023Quote: ... were grown on 10-mm coverslips previously coated with poly-D-lysine (0.1 mg/mL, 1 h, 37 °C, Gibco) placed in a well on a 24-well plate ...
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bioRxiv - Biophysics 2023Quote: ... for 1 h at room temperature and developed with SuperSignalTM West Pico PLUS chemiluminescent substrate (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... and goat-anti-rabbit-IgG-H-L-highly-cross-adsorbed-secondary-antibody-polyclonal (Thermo Fisher Scientific, A11004, 1:700).
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bioRxiv - Neuroscience 2023Quote: ... We used the following secondary antibodies: Goat anti-guinea pig (IgG H+L) A647 (1:500, Invitrogen, #A-21450), Goat anti-Rat (IgG H+L ...
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bioRxiv - Genetics 2024Quote: ... embryos were then incubated with Goat anti-Mouse IgG (H+L) Alexa Fluor 488 (1:150, Cat# A28175, Invitrogen) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1 h at room temperature in a PBS solution containing 0.2 µg/mL Hoechst 33342 (H3570, ThermoFisher) and 1 µg/mL Streptavidin-Alexa488 (016-540-084 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 x 10 minutes in PBS and incubated for 2 hours at RT with the following secondary antibodies (1:500): goat anti-mouse IgG (H+L) cross-adsorbed Alexa Fluor 546 (RRID: AB_2534071, A-11003, ThermoFisher), goat anti-mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... and pieces incubated in secondary antibodies [anti-rat secondary antibody (H+L) 1:400 (Thermo Scientific, Alexa Fluor 488), anti-rabbit 568 secondary antibody (H+L ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 μg of total RNA was used for cDNA synthesis using Maxima H Minus Master Mix (Thermo Scientific) in a reaction volume of 5 μL ...