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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... counterstaining with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) or propidium iodide (PI ...
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bioRxiv - Bioengineering 2023Quote: ... The 4′,6-diamidino-2-phenylindole (DAPI) was acquired from Invitrogen, Thermofisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... together with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) diluted in 2% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... or 4°,6-diamidino-2-phenylindole (DAPI, 5 μg/ml, Invitrogen) were added during the first wash step to visualize nuclei ...
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bioRxiv - Physiology 2023Quote: ... DAPI (4’,6-diamidino-2-phenylindole) (D1306) was purchased from Invitrogen. XMU-MP1 (#22083 ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and mounted on to glass slides in FluorSave™(Millipore) ...
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bioRxiv - Genomics 2023Quote: ... Cells were blocked for 1-2 hrs using 2% (v/v) Bovine Serum Albumin (BSA, Thermo Scientific, Cat # 37525) + 5% FBS in HBSS ...
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bioRxiv - Molecular Biology 2024Quote: Vero E6 cells growing in P24 wells were infected with SARS-CoV-2 (Wuhan-1) at a moi = 2 pfu/cell in 0.5 mL OPTIMEM (Gibco) under biosafety level 3 conditions ...
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bioRxiv - Immunology 2024Quote: ... the inoculum was replaced with 150 μL of overlay medium (1:1 mix of Leibovitz’s L-15 medium supplemented with 0.1% pen/strep, 2% TPB, 1× NEAA, 2× Antibiotic-Antimycotic [Life Technologies] ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL malt extract (2% w/w) in 4 mL screw neck vials (Fisher Scientific, Loughborough, UK) were inoculated with an agar piece of the G ...
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bioRxiv - Cell Biology 2024Quote: ... adipose tissue (2∼4 cm3) was washed twice with PBS solution (AQ) containing 2% penicillin-streptomycin (Invitrogen), and then cut to 1∼2 mm3 with scissors ...
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bioRxiv - Biophysics 2023Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes). Stained sections were imaged using epifluorescence and deconvolution epifluorescence microscopy on an Olympus IX83 ...
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bioRxiv - Bioengineering 2023Quote: ... Then the cells were stained with a solution of 4 mM calcein AM (TRC, #C125400) and 4 mM ethidium homodimer-1 (Thermo Fisher, #L3224) dissolved in PBS to identify live and dead cells ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with secondary antibodies in vehicle overnight at 4 °C (Alexa flor 488 goat anti mouse, Invitrogen A11001, 1:1000; Alexa flor 546 goat anti rabbit, Invitrogen A11035, 1:500). After 3 washes for 30 minutes in PBS ...
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bioRxiv - Immunology 2021Quote: ... The phage/serum mixtures were rotated overnight at 4°C after which 40 μL of a 1:1 Protein A/G coated magnetic bead mixture (Invitrogen, #10002D and #10004D) was added to each well and rotated for an additional 4 hours at 4°C to capture all IgG ...
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bioRxiv - Cell Biology 2020Quote: ... then placed on an agarose pad (4% agarose in sterile water mixed 1:1 with 10%FBS in DMEM (Gibco, Cat. No.11965), and with doxycyline (2 μg/ml)) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated with anti-KLK10 (BiossUSA bs-2531R, 1:100) primary antibody overnight at 4°C followed by Alexa Fluor-647 (ThermoFisher Scientific, 1:500) secondary antibody for 2hrs at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... -GFAP (1:500, SynapticSystems) -Aquaporin-4 (1:200, Alomone Labs) as well as with Click-iT™ EdU Cell Proliferation Kit (Thermo Fisher Scientific) according to manufactures protocol ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Cell Biology 2024Quote: ... 3D or in suspension as described above were incubated with cell-impermeable 4 µM ethidium homodimer 1 (EthD-1; Invitrogen catalogue no. E1169) and 10 µg/ml cell-permeable Hoechst (Thermo Fisher catalogue no ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated for 1 h at RT with primary antibodies as follows: rabbit anti-Tubulin (PA5-19489, Invitrogen), mouse anti-Tubulin (B-5-1-2 ...
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bioRxiv - Cell Biology 2019Quote: ... alexa 647-conjugated goat anti-rabbit IgG (H+L) antibody (no. A-21245, Thermo Fisher Scientific, 1:1,000 dilution); and alexa 568-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... goat anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (1/500, Invitrogen #A-11008). Cells were washed 3 x 5 min and incubated for 10 min with DAPI (1/2000 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and incubated in anti-goat or anti-rabbit IgG (H+L) Alexa 488-conjugated secondary antibodies (Invitrogen, 1:750).
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bioRxiv - Immunology 2021Quote: ... 0.1% Tween-20) and incubated for 1 h with HRP-conjugated mouse or rabbit secondary antibody solutions (Thermo Fisher) diluted in 5% milk (diluted 1/10000) ...
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bioRxiv - Pathology 2019Quote: ... sections were incubated in a cocktail of 1:400 Goat anti-Rabbit IgG (H+L) (A-11008, Life Technologies) and 1:400 AF546 Goat anti-Mouse IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... overnight at RT followed by Alexa Fluor 488 goat anti-rabbit IgG (H+L) (1:250, #A11008, Life Technologies), Alexa Fluor 647 donkey anti-goat IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... overnight at RT followed by Alexa Fluor 488 goat anti-rabbit IgG (H+L) (1:250, #A11008, Life Technologies), Alexa Fluor 635 goat anti-mouse IgG (H+L ...
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bioRxiv - Genetics 2021Quote: ... then incubated with donkey anti-rabbit IgG (H+L) Alexa Flour 488 (Thermo Fisher Scientific, A-21206; 1:2,000) and anti-mouse IgG (H+L ...
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bioRxiv - Plant Biology 2020Quote: ... sections were incubated for 1 h with the secondary antibody Alexa Fluor 546 goat anti-rat IgG (Life Technologies) diluted 1:1,000 in 0.1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Incubation with primary antibodies was conducted for 48 h in the presence of 1% BSA (Super blocker, ThermoFisher Scientific). Incubation with secondary antibodies was conducted for 24 h in the presence of 1% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... combined with the secondary antibody Alexa Fluor® 594 goat anti-Rabbit IgG (H+L) (Molecular probes, 1:500). Samples were washed with PBS (five minutes ×3 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated for 1.5 h at RT with a Alexa 488 or Cy3-conjugated secondary antibody (1:300; Invitrogen). The stained sections were examined with a confocal microscope and images were captured with a CCD spot camera ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary HRP-conjugated antibodies used were: Pierce peroxidase goat antimouse IGG (H+L) (ThermoFisher, catalogue no. 31432; 1:4000) and Pierce peroxidase goat antirabbit IGG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Goat anti-rabbit IgG (H+L) Alexa Fluor A488 (1:500) (A11029) All secondary antibodies were from LIFE TECHNOLOGIES, Oregon ...
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bioRxiv - Neuroscience 2020Quote: ... the peptides from each sample were reacted for 1 h with the appropriate TMT-NHS isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human LMP7 (ThermoFisher Scientific; MA5-15890) or PRDX6 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (1:250, Molecular Probes A11034). To test the specificity of the antibodies ...
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bioRxiv - Pathology 2021Quote: Cryosections were permeabilized and blocked for 1 h at RT in 0.1% PBTx and 10% fetal calf serum (FCS; Invitrogen), and incubated O/N at 4 °C with the primary antibodies in 0.1% PBTx plus 1% FCS ...
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bioRxiv - Cancer Biology 2020Quote: ... and Alexa Fluor™ 594 goat anti-mouse IgG (H+L) (Cat no. A-21203, 1:500, ThermoFisher Scientific). Secondary antibodies for Western blotting were Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 3X with PBST at RT for 1 h and mounted with SlowFade Gold antifade reagent (ThermoFisher). Samples were analyzed using a Nikon inverted TE2000-E microscope equipped with phase contrast and epifluorescence ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by a 1 h incubation at room temperature with Dynabeads Protein G magnetic beads (Thermo Fisher Scientific, #10003D). Beads were washed three times with lysis buffer and eluted by boiling in SDS sample buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (1/500, Invitrogen #A-11008). After 3×10 min of washing in PBS 1X + 0.1% Triton X-100 + 1% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... We used the following secondary antibodies: Goat anti-guinea pig (IgG H+L) A647 (1:500, Invitrogen, #A-21450), Goat anti-Rat (IgG H+L ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ T-cells were stained with LysoTracker Red DND-99 (Invitrogen; 5 μM; 1 h; 37°C), fixed (3% PFA ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthetized by reverse transcribing 1 µg of total RNA using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... for 1 h at 37°C and RNA was subsequently purified by GeneJET RNA Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The cells were washed 3 times in PBS 0.05% Triton X-100 and incubated 1 h with anti-mouse AlexaFluor 488 and anti-rabbit AlexaFluor 568 (Invitrogen) diluted 1:500 in MAXblock medium with 1 μg/ml DAPI (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... After 4 h this buffer was replaced with Neurobasal/B27 culture medium containing 0.5 mM glutamine (50:1 by volume: Invitrogen), and 2 days later cytosine arabinoside (1 μM ...
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bioRxiv - Neuroscience 2022Quote: ... cells were permeabilized and blocked for 1 h at RT using 0.1% Triton X-100 (Thermo Fisher Scientific, 28313) and 4% normal donkey serum (Jackson Immuno Research ...