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Citations for Thermo Fisher :
3651 - 3700 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The blot was blocked in Intercept PBS blocking buffer (Li-cor) and subsequently incubated with RFP monoclonal antibody (ThermoFisher, MA5-15257) and probed with fluorescent anti-mouse secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific, G46-1) in PBS for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... three times and then blocking buffer (5% normal goat serum [Cat #PCN5000, ThermoFisher], 0.2% Triton™ X-100 [Cat #9002-93-1, Fisher Scientific] ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were rinsed with PBS and incubated in blocking solution for one hour with the appropriate secondary antibody conjugated to an Alexa-Fluor (4ug/mL, Thermo Fisher) along with the nuclear stain DAPI (Sigma ...
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bioRxiv - Immunology 2021Quote: ... were coated with 50 μL of 2 μg/mL per well of SARS-CoV-2 FL-S protein overnight at 4 °C prior to washing in PBS/Tween (0.05% v/v) and blocking with Blocker Casein in PBS (Thermo Fisher Scientific) for 1 h at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... They were then incubated with the appropriate dilution of primary and secondary antibodies (or nanobody) in blocking buffer consisting of 3% (wt/vol) BSA and 0.2% Triton X-100 (vol/vol; Thermo Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2021Quote: ... Plates washed with PBS-Tween (PBS-T) and non-specific binding was blocked with TBS Startblock blocking buffer (Thermo Fisher Scientific). Serum samples were serially diluted 3-fold starting with a 1:50 dilution and added to the coated plates ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 (Invitrogen, Carlsbad, CA; 1:500).
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT: anti-Rabbit 555 (1:800; Invitrogen; A31572), anti-Chicken Cy2 (1:800 ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates followed by TMB substrate (Thermofisher Scientific). Upon stopping the reaction with 1M H2SO4 ...
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bioRxiv - Neuroscience 2020Quote: ... Isolated capillary networks were placed onto a glass coverslip and fixed (4% formaldehyde in phosphate buffered saline (PBS)) and blocked (0.5% Triton X-100, 5% SEABlock Blocking Buffer (ThermoFisher Scientific, USA) in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by incubation in goat anti-mouse Texas Red secondary antibody (20 μg/mL in blocking solution, Molecular Probes, Merelbeke, Belgium) and then in goat antirabbit FITC secondary antibody (10 μg/ml in blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... 3 times and the following secondary antibodies diluted in blocking buffer were used: Goat anti-rabbit Alexa Fluor 488 (1:500; Thermo Fisher), Cy3-conjugated AffiniPure donkey anti-mouse (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Retinas were then washed five times with 1:1 blocking buffer in PBS for 20 min and incubated with Alexa Fluor streptavidin-conjugated antibodies (1:100; Life Technologies) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with blocking buffer (10% normal goat serum–50062Z, Thermo Fisher; 0.5% BSA–BP1600, Thermo Fisher; 0.2% Triton X-100-T8787, Thermo Fisher) at room temperature for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Permeabilized embryos were incubated in blocking buffer (0.2% Triton X-100 in PBS + 2% BSA (BSA Fraction V 7.5%, Gibco, cat.# 15260-037) + 5% goat serum (Jackson Immunoresrearch/ Dianova ...
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bioRxiv - Genomics 2022Quote: Samples were then blocked for 1 hour at room temperature in Blocking Solution (1x PBST, 10% Molecular-grade BSA (ThermoFisher AM2616)) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were stained overnight with the following primary antibodies diluted in blocking buffer: mouse anti-neurofilament-M (1:1500, RMO270, Invitrogen, RRID:AB_2315286); rabbit anti-6xHisTag (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked with 5% fat-free skim milk in TBST (Tris buffer saline with 0.05% Tween 20) or SUPERBLOCK T20 (TBS) Blocking Buffer (Thermo Fisher Scientific) then incubated with appropriate primary antibody overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Triton-X in PBS for three minutes followed by blocking with blocking buffer (1% w/v bovine serum albumin with 0.2% w/v keyhole limpet hemocyanin in PBS; Thermo Fisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with blocking solution for a total of 20 minutes and then incubated with secondary antibody (1:500, Invitrogen, #A31572) for 2h ...
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bioRxiv - Microbiology 2022Quote: ... The primary anti-body was washed away in PBS containing 1% DMSO (wash buffer) and larvae were incubated in blocking solution containing Goat anti-Rabbit546 (ThermoFisher #A11010) at 1:1000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were washed twice in blocking buffer and twice in TBS before mounting with ProLong™ Glass Antifade Mountant (Invitrogen P36982). Samples were visualized with a Zeiss LSM 710 META inverted laser scanning confocal microscope in part through the use of the Vanderbilt Cell Imaging Shared Resource ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was washed in blocking solution and treated with secondary antibody (anti-guinea pig 488 1:50 and anti-chicken 594 1:300, Invitrogen, WA316328) for 2-3 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then washed 3 times in cold PBS and blocked in FISH blocking buffer (2x SSC, 40ng/μL yeast tRNA (Life Technologies), 40ng/μL salmon sperm DNA (Life Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
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bioRxiv - Cell Biology 2024Quote: ... they were rinsed three times in PBS and blocked for 30 minutes in blocking solution (PBS + 5% by volume normal goat serum, ThermoFisher PCN5000). Staining was for 1 hour with 1:500 anti-OSBP (Atlas Antibodies HPA039227 ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated with blocking solution (1× PBS with 5% Normal Goat Serum [50-062Z; ThermoFisher Scientific], 0.1% Triton X-100) for 15 min and with primary antibodies solutions for 2 to 3 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated for 72-hours in the same blocking solution with anti-FR (1:500, Invitrogen #A6397, made in rabbit) and either anti-TH (1:5K ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked with PLA blocking buffer and incubated with anti-NOD2 antibody (#MA1-16611, ThermoFisher, 1:500: RRID: AB_568643), anti-α-syn aggregate antibody (#ab209538 ...
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bioRxiv - Neuroscience 2024Quote: BV-2 cells were fixed with 4% PFA for 10 minutes and subsequently incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2024Quote: The blocking solution for all immunostaining approaches was 1X PBS (no Ca2+ / Mg2+) with 1% normal donkey serum (Fisher Scientific; OB003001), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... donkey anti-sheep antibodies were incubated for 1h and after thorough washing and blocking with 5% normal goat serum (Life Technologies) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with PBS and incubated with Alexa Fluor-488 conjugated goat anti-mouse IgG secondary antibody diluted at 1:500 in blocking solution (Invitrogen, USA). Fluorescent foci were visually counted (in a well with a dilution that produced <60 total foci ...
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bioRxiv - Neuroscience 2022Quote: ... then processed for 48 hours at 4 degrees with secondary antibodies in blocking solution (1:1000, Alexa Fluor 647 goat anti-rabbit, Jackson Laboratories, plus 1:500 NeuroTrace, Invitrogen). Tissue sections were then rinsed again 3×10 minutes in PBS ...
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bioRxiv - Neuroscience 2023Quote: Immunohistochemistry was performed as follows: brain sections were incubated in a blocking solution consisting of 5% normal goat serum (Thermo Fisher) and 0.1% Triton (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... cells were permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h; Thermo Fisher Scientific, USA). Overnight incubation with primary antibodies (anti-VPS36 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206 ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were diluted with odyssey blocking buffer in tris-buffered-saline containing 0.1% Tween20 (TBS-T) (Thermo Fisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The lentiviral constructs pHIV-dTomato-CDK19_CasR and pHIV-dTomato-CDK19M_CasR that contain the coding sequences of human CDK19-wild-type or CDK19-D173A kinase-inactive mutant with mutations introduced in the sgRNA-targeting sequence and proximal PAM sequences (blocking sgRNA targeting but not changing the coded protein sequences) were generated using the Phusion Site-Directed Mutagenesis Kit (Thermo Scientific) with the hCDK19-CasR-F/R primers (Table S5 ...
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bioRxiv - Bioengineering 2023Quote: ... To minimize nonspecific binding the samples were blocked for 4 h at room temperature with a blocking buffer containing 1% of Tween20 (Fisher Scientific) and 3 % of BSA (VWR ...
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bioRxiv - Biochemistry 2023Quote: ... UV-crosslinked at 120 mJ and blocked for 2 hours rotating at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Thermo Fisher). Blots were probed rotating overnight at 42°C with 32P-labeled DNA probes prepared as described below ...
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 in blocking buffer for 1h and counterstained with DAPI prior to mounting with ProLong Gold anti-fade reagent (Invitrogen, P36930) for subsequent immunofluorescence analysis.
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bioRxiv - Genomics 2023Quote: ... Then DNA oligo-conjugated primary antibodies were incubated in the blocking solution with 100-fold diluted SUPERase In RNase Inhibitor (Invitrogen AM2694) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized with 0.01% Triton X-100 in PBS for 5 min at room temperature and then treated with BlockAid Blocking Solution (Thermo Fisher) supplemented with FcR Blocking Reagent (Miltenyi ...
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bioRxiv - Microbiology 2023Quote: ... + 0.05% Tween 20 and blocked with 4% milk powder in PBS (Regilait) in the first panning round and Pierce protein free blocking buffer (Thermo Scientific), SEA blocking buffer (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... representative brain sections were wasted three times in 1× PBS and incubated in a 24 well plate at room temperature for one hour in blocking buffer: 10% goat serum (Gibco, 16210064), 1% BSA (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... samples were blocked for 15 min in blocking buffer and then incubated with Alexa Fluor conjugated primary antibody against phospho-Src (Tyr419) (44-660G, ThermoFisher Scientific). Finally ...