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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The titration was performed in a black 96 well plate (Thermo-Fisher scientific) that allows the measurement of multiple data points and facilitating the titration curve ...
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bioRxiv - Biochemistry 2024Quote: ... using Lipofectamine 2000 (Thermo Fisher, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble fraction was incubated overnight at 4°C with Pierce™ Anti-DYKDDDDK Affinity Resin (Thermo Fisher) and purified according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... was used as a template to synthesize Cas9 mRNA in vitro using the mMESSAGE mMACHINE™ T3 Transcription Kit (Invitrogen, AM1348). To generate F0 mosaic mutant animals ...
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bioRxiv - Biochemistry 2024Quote: ... or 15N-Met (149.0386 - 148.0430) was analyzed using the Vanquish UHPLC system coupled with the Q Exactive quadrupole-Orbitrap MS (Thermo Fisher Scientific, Waltham, MA) with negative ionization using settings as previously described117 to determine the utilization of kynurenine or Gln ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were stained with 0.165 uM AlexaFluor488-phalloidin (ThermoFisher) for 20 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Glia were passaged at 37°C and 5% CO2 in complete media (Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific, 11885), 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... placed in ice-cold Leibovitz’s L-15 medium (Gibco), and digested in Collagenase type II (4 mg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... Peptide concentration was determined with the Nanodrop A280 assay and/or the BCA assay (Thermo Scientific) and adjusted to 0.02 g/L with 0.1 % formic acid before loading 20 μL (400 ng ...
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bioRxiv - Bioengineering 2024Quote: ... all reagents were purchased from ThermoFisher (Waltham, MA). Human microvascular endothelial cells (hMVEC ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was then cut out using a scalpel and placed on a standard glass slide and mounted with Vectashield antifade mounting media (Fisher Scientific, NC9265087) with a cover slip placed on top ...
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bioRxiv - Bioengineering 2024Quote: ... 1% Penicillin– Streptomycin (ThermoFisher Scientific, 15,140,122), 21 μg/ml Bovine Pituitary Extract (BPE ...
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bioRxiv - Bioengineering 2024Quote: All reagents were purchased from ThermoFisher (Waltham, MA) unless stated ...
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bioRxiv - Bioengineering 2024Quote: ... 10% fetal bovine serum (FBS, ThermoFisher Scientific, 160,000,036), 1% Penicillin– Streptomycin (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Cell metabolic activity was quantified in 2D models using an MTT assay (Thermo Fisher, Waltham, MA). For the MTT assays ...
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bioRxiv - Bioengineering 2024Quote: ... Data were processed using Excalibur software (Thermo Scientific). Both lactic acid and di-lactic acid (m/z 161.045 ...
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bioRxiv - Bioengineering 2024Quote: ... The remainder was fractionated with Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific), concentrated to dryness ...
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bioRxiv - Bioengineering 2024Quote: Stained films were inverted onto a coverslip in a drop of Slow Fade Diamond Antifade Mountant (Invitrogen, S36972). Imaging was performed using a Leica DMi8 microscope ...
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bioRxiv - Bioengineering 2024Quote: ... AM (Invitrogen, C1429) for 20 min ...
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bioRxiv - Bioengineering 2024Quote: ... phosphomolybdic acid hydrate 80% (Acros Organics); Picrosirius Red ...
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bioRxiv - Bioengineering 2024Quote: ... Qubit 3 (Fisher Scientific) and 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... The PowerUp™ SYBR™ Green Master Mix (Invitrogen) was used with human primers for Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) (HyClone) and 1ξ penicillin-streptomycin (pen/strep) (Fisher Scientific). A549 cells were maintained in Hams F-12 medium (Corning) ...
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bioRxiv - Bioengineering 2024Quote: ... Capture antibodies (provided in Supplementary Table 1) were biotinylated using the EZ-Link micro Sulfo-NHS-biotinylation kit from (Thermo Fisher Scientific). The biotinylated antibodies were then diluted to 10 μg/mL in a 1 % BSA solution and added to the grids for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then detached from wells using 300 µL of 0.25% Trypsin containing 1ξ EDTA (Fisher Scientific) for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... if necessary and washed three times in Phosphate-Buffered Saline (PBS) supplemented with 2% Penicillin / Streptomicin (10,000 U/mL, Gibco) and Primocin (100 µg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA). Frozen C2C12 myoblasts were purchased from ATCC (Manassas ...
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bioRxiv - Bioengineering 2024Quote: ... Live imaging medium for cell lines consisted of phenol red-free DMEM (4.5 g l-1 glucose, L-glutamine, and 25 mM HEPES, #21063-029, Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... Frozen C2C12 myoblasts were purchased from ATCC (Manassas, VA) and were grown in 89% Gibco Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA) supplemented with high glucose ...
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bioRxiv - Bioengineering 2024Quote: ... 10% Gibco Fetal Bovine Serum (Thermo Fisher Scientific, Waltham, MA), and 1% penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 30-minute fixation in 4 wt% paraformaldehyde (Thermo Fisher Scientific, Waltham, MA); 3 PBS washes ...
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bioRxiv - Bioengineering 2024Quote: ... and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA). All cells were used between passages 1 and 4.
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bioRxiv - Bioengineering 2024Quote: ... Samples were then frozen using a Vitrobot Mark IV Plunge System (Thermo Fisher Scientific, Waltham, MA) and loaded into a 626 Single tilt liquid nitrogen cryo-transfer holder (Gatan Inc ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were imaged on standard glass microscopy slides (Fisher Scientific, Pittsburgh, PA) at a consistent angle.
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bioRxiv - Bioengineering 2024Quote: ... Total protein was then quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher, USA). Per the vendor’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... and anti-CD4 (1:200; 14-0042-82, Invitrogen; Carlsbad, CA, USA) primary antibodies ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... Purified T-cells (5 x 104 cells/well) were stimulated in vitro using αCD3/αCD28 stimulation beads (ThermoFisher Scientific, # 11456D) at 1 µg/mL and 5 µg/mL ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... and Near IR Live/dead stain (Invitrogen, Thermo, # L3495). Following 24h and 48h co-culture ...
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bioRxiv - Bioengineering 2024Quote: Primary human hepatocytes were transfected 1 day after thawing using Lipofectamine MessengerMAX Transfection Reagent (Invitrogen) using the following protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The mRNA was subsequently column purified using MEGAClear transcription clean-up kit (ThermoFisher) and mRNA purity analyzed using a fragment analyzer (Agilent).
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 488 and 568 conjugated goat anti-rabbit or anti-rat IgG antibodies (1:500 each; Invitrogen; Carlsbad, CA, USA) were used for fluorescent signal detection ...
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bioRxiv - Bioengineering 2024Quote: A separate set of ATA samples (n=4 per group) were immersed in optimal cutting temperature compound (OCT; 4585, Thermo Fisher Scientific; Waltham, MA, USA) immediately after excision and frozen at 80◦C for fluorescent immunostaining ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were quantified using a Qubit 4 Fluorometer (Life Technologies) and subjected to sequencing using Illumina NextSeq system according to the manufacturer’s instructions.
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... wells were washed with with PBS and αWAVE1 (ThermoFisher Scientific, # PA5-78273) was added at a 1:100 dilution in PBS containing 0.5% BSA and 0.01% Triton-X 100 for 1 hr at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons were generated with Phusion Flash High-Fidelity 2x Mastermix (F548, Thermo Scientific) or Q5 Hot Start High-Fidelity 2x Mastermix (M0492 ...
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bioRxiv - Bioengineering 2024Quote: ... All reagents were purchased from Gibco. Cells were maintained at 37 °C in a 5% CO2 atmosphere and regularly tested for mycoplasma contamination ...
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bioRxiv - Bioengineering 2024Quote: ... Phenylephrine (L-Phenylephrine hydrochloride, 99%, Thermo Fisher Scientific; Waltham, MA, USA) was added to the bath at a concentration of 2·10−6 M to induce sub-maximal vasoconstriction over 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... using the following protocol: Lipofectamine solution was prepared by mixing 0.3 μL of Lipofectamine MessengerMAX with 4.7 μL of optiMEM (Gibco) and incubated at room temperature for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Increasing doses of acetylcholine (Acetylcholine chloride, 99%, Thermo Fisher Scientific; Waltham, MA, USA) ranging from 10−9 to 10−4 M were administered sequentially through the pressurized luminal loop to probe for endothelium-dependent vasodilatation ...