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Citations for Thermo Fisher :
3801 - 3850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... EDTA were purchased from Fisher Scientific. Glycerol and ATP-Mg ...
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bioRxiv - Biochemistry 2024Quote: ... coverslips (25 mm, Fisher Scientific) were cleaned by bath sonication in 2% Hellmanex ...
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bioRxiv - Biochemistry 2024Quote: ... deglycosylated GCase and the GCase N370S mutant were solid-phase coated on the bottom of a 96-well ELISA plates (Maxisorp Nunc-immuno plate ...
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bioRxiv - Biochemistry 2024Quote: ... Using a QuantStudio 3 RT-PCR thermocycler (Invitrogen), the DSF samples were incubated at 5°C for 1 min ...
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bioRxiv - Biochemistry 2024Quote: ... and 1% non-essential amino acids (all Thermo Fisher Scientific, Rockford, IL, USA) at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescent lipids used in the study were Marina Blue™ 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (Marina Blue™ DHPE) (Invitrogen) and Forte Blue DHPE (AAT Bioquest).
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bioRxiv - Biochemistry 2024Quote: ... 100 ng) was transiently transfected into cells in Opti-MEM media (HiMedia) with Lipofectamine 2000 (Invitrogen). Each well was also transfected with a pGL3 vector (10 ng ...
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bioRxiv - Biochemistry 2024Quote: Cryo-EM data were collected at the Umeå Core Facility for Electron Microscopy employing a 200 kV Glacios system (Thermo Fisher Scientific) equipped with a Falcon 4i direct electron detector ...
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bioRxiv - Biochemistry 2024Quote: ... LIG1 proteins were then fluorescently labeled using amine reactive NHS ester conjugated Alexa Fluor (AF)488 according to the manufacturer’s instructions (Thermo Fisher). The protein concentrations and labeling efficiency were finally determined by calculating the molar ratio of dye to protein within the solution by measuring absorbance at 280 nm and 488 nm.
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bioRxiv - Biochemistry 2024Quote: ... 5% glycerol (Thermo Fisher Scientific), 8 mM MgCl2 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... we used Refeyn Acquire MP and DiscoverMP software and NativeMarkTM (ThermoFisher) was used as standard.
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bioRxiv - Biochemistry 2024Quote: ... and a pixel size of 0.75 Å were collected using EPU (Thermo Fisher Scientific). We summarized the data collection statistics in S2 Table.
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bioRxiv - Biochemistry 2024Quote: ... Eluted peptides were analyzed on the Orbitrap Fusion mass spectrometer (Thermo Scientific) in data-dependent mode with dynamic exclusion enabled (60 s exclusion time) ...
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bioRxiv - Biochemistry 2024Quote: ... Grids were blotted for 2 s under 95% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Cryo-EM datasets were collected on a Titan Krios electron microscope operating at 300 kV and equipped with Thermo Fisher Falcon 4 direct electron detector at the facilities of the University of Leeds ...
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bioRxiv - Biochemistry 2024Quote: ... 8 mM MgCl2 (Thermo Fisher Scientific), 2 mM ATP (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... was incubated for 10 minutes at room temperature (∼22 °C) in reaction buffer containing the following components at their final concentrations: 25 mM Tris (pH 8; Thermo Fisher Scientific), 150 mM KCl (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Quantifoil R2/2 200-mesh copper grids with 2 nm carbon (EM sciences) at 4 °C and 100% humidity using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). The sample was blotted for 5s ...
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bioRxiv - Biochemistry 2024Quote: ... SML0560) and dissolved in ultrapure water (Invitrogen) at a high stock concentration of 20 mM and stored in the dark at 20 °C.
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bioRxiv - Biochemistry 2024Quote: ... DT or CT1 proteins were diluted in 150mM citrate phosphate buffer containing 5X SYPRO Orange (Invitrogen). The pH of the buffer varied from 4.0–7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... and biotinylated PDCoV SIL121_2014 ectodomain labeled with both PE and Alexa-Fluor 647 streptavidin (Life Technologies). Sorted IgG+ memory B cells were seeded at clonal dilution in 384-well plates an a monolayer of feeder mesenchymal cells in the presence of B cell survival factors ...
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bioRxiv - Biochemistry 2024Quote: ... total RNA was purified from HeLa cells using TRIzol RNA isolation Reagent (Invitrogen). Proteins (50 µM ...
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bioRxiv - Biochemistry 2024Quote: ... 1X Vitamin Stock (Gibco), 1 mg D-Biotin and trace metals ...
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bioRxiv - Biochemistry 2024Quote: Nunc MaxiSorp™ plates (Thermo Fisher Scientific) were immobilized with 1ug of protein overnight at 4C ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were grown in high glucose DMEM (ThermoFisher Scientific) supplemented with 10% Heat Inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: CryoEM samples were prepared by applying protein to CFLAT 2/2 holey carbon grids (Protochips) and blotting away liquid four times successively before plunging into liquid ethane using a Vitrobot (ThermoFisher). Samples used to determine R-state structures contained 9 µM PFKL ...
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bioRxiv - Biochemistry 2024Quote: ... immobilized on Dynabeads protein G (Invitrogen), for 60 minutes at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and FLAG-PFKL-N702T were cloned into a pLenti6.3 vector (ThermoFisher Scientific). The presence of the mutations and the integrity of the constructs were verified by Sanger sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... the medium was replaced one containing 900 µg/ml geneticin (G418, Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA). The medium was changed every three days ...
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bioRxiv - Biochemistry 2024Quote: ... with B-27 supplement (GIBCO, cat# 17504001) and 1x Glutamax-I (GIBCO ...
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bioRxiv - Biochemistry 2024Quote: ... MitoTracker® Deep Red FM (Invitrogen, M22426) at a final concentration of 125 nM was added to the growth media of cells prior to fixation ...
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells (ATCC, CRL-1573) were subcultured every 3-4 days in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, 41965-039) supplemented with 10% v/v fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... and 1x penicillin-streptomycin (Gibco) at 26°C under humidified normal atmosphere ...
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bioRxiv - Biochemistry 2024Quote: ... AHR activation was determined in the AZ-AHR cell line.45 Kc167 cells were propagated in the Schneider’s Drosophila medium (Gibco, 21720-024) supplemented with 10% heat-inactivated fetal bovine serum (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... Hoechst counterstain (Invitrogen) was included in the final mix for cell nuclei staining ...
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bioRxiv - Biochemistry 2024Quote: ... The following secondary antibodies were used at a 1:10,000 dilution (species and vendor indicated in parenthesis): anti-rabbit Alexa Fluor 680 (goat, Invitrogen cat# A21077); anti-mouse Alexa Fluor 680 (goat ...
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bioRxiv - Biochemistry 2024Quote: ... The qPCR was run on a ViiA 7 Real-Time PCR machine (Applied biosystems) using the following conditions ...
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bioRxiv - Biochemistry 2024Quote: ... The AriAEQ protein sample was applied to the grid in a 3.5 μL drop within the environmental chamber adjusted to 4°C temperature and approximately 95% humidity in a Vitrobot Mark IV (ThermoFisher Scientific). After a 5 seconds incubation ...
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bioRxiv - Biochemistry 2024Quote: ... The AriAEQ-AriBE90A complex sample was applied to the grid in a 3 μL drop within the environmental chamber adjusted to 4°C temperature and approximately 95% humidity in a Vitrobot Mark IV (ThermoFisher Scientific). After a 4 second incubation ...
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bioRxiv - Biochemistry 2024Quote: The samples for blue native PAGE (BN-PAGE) analysis were prepared using a Native PAGE Sample Prep Kit (Invitrogen). For BN-PAGE ...
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bioRxiv - Biochemistry 2024Quote: Grids were mounteds into standard AutoGrids (ThermoFisher Scientific) for imaging ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mg ml−1 Streptomycin (1X Pen/Strep) and 1 X non-essential amino acids (Life Technologies). HeLa Flp-In TREx cells were cultured using DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Biochemistry 2024Quote: ... Automated data acquisition was performed using EPU (ThermoFisher Scientific). Movies were collected at a magnification of 130,000x and a pixel size of 0.935 Å ...
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bioRxiv - Biochemistry 2024Quote: ... anti-mouse Alexa Fluor 680 (goat, Invitrogen cat# A21058); anti-mouse IRDye 800 (goat ...
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bioRxiv - Biochemistry 2024Quote: ... RNA was extracted using the PureLink RNA Mini kit (ThermoFisher cat# 12183018A) and stored at -80°C ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 μl of 2X SYBR Green PCR Master Mix (Applied biosystems cat# 4385612). For each gene target ...
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bioRxiv - Biochemistry 2024Quote: ... BN-PAGE was performed by Native PAGE Running Buffers (Invitrogen). The gels were transferred on to PVDF membranes for IB analysis ...
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bioRxiv - Biochemistry 2024Quote: Grids were mounteds into standard AutoGrids (ThermoFisher Scientific) for imaging ...
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bioRxiv - Biochemistry 2024Quote: ... Imaging was done using a Titan Krios G4 transmission electron microscope (ThermoFisher Scientific) operated at 300 kV configured for fringe-free illumination and equipped with a Falcon 4 direct electron detector with Selectris X energy filter ...
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bioRxiv - Biochemistry 2024Quote: ... Automated data acquisition was performed using EPU (ThermoFisher Scientific). Movies were collected at a magnification of 130,000x and a pixel size of 0.935 Å ...
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bioRxiv - Biochemistry 2024Quote: ... and sodium deoxycholate (Acros Organics) to a final concentration of 0.5 mg/mL then incubated overnight at 4°C ...