Labshake search
Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Hank’s balanced salt solution (HBSS) and HEPES buffer were purchased from Life Technologies. TeNT46 was synthesized by GL Biochem Ltd ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were transiently transfected at 50-60% confluency with plasmid expressing protein of interest (Table 1) and/or target specific siRNA using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: Cells were seeded onto LabTek-II imaging chambers (Nalge Nunc, Thermo Fisher) before fixation ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were incubated with 0.1 µm Tetraspeck microspheres (Invitrogen, Thermo Fisher) in PBS to use as a fiducial marker ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were incubated with 0.1 µm Tetraspeck microspheres (Invitrogen, Thermo Fisher) in PBS to use as a fiducial marker ...
-
bioRxiv - Cell Biology 2024Quote: ... HEK-293 cells with 80% of confluency were transfected using 250ng/ml of pCMVHA hEZH2 and Lipofectamine 2000 (#11668030, Invitrogen, Thermo Fisher Scientific) for 4 hrs ...
-
bioRxiv - Cell Biology 2024Quote: ... All culture media (GIBCO Laboratories, Grand Island, NY) was supplemented with 10% (vol/vol ...
-
bioRxiv - Plant Biology 2024Quote: ... and treated with Turbo DNase (Ambion), according to the manufacturer’s specifications ...
-
bioRxiv - Plant Biology 2024Quote: ... Stable transformants were selected using 100 µgml-1 Zeocin (Invitrogen; R25001). Genotyping was performed using the primers putativeSNOG1A_genomic2_F and putativeSNOG1A_genomic2_R (Table 1 ...
-
bioRxiv - Plant Biology 2024Quote: ... Isobaric labeling was done using 18-plex TMTpro reagents (Thermo Scientific A52047) as follows ...
-
bioRxiv - Plant Biology 2024Quote: A U3000 HPLC directly coupled using a Nanospray Flex ion source to Q-Exactive Plus high- resolution quadrupole Orbitrap mass spectrometer (Thermo Scientific) was used for LC-MS/MS ...
-
bioRxiv - Plant Biology 2024Quote: ... pH 8.5 (Invitrogen). Columns were centrifugated at 12,000xg for 10 minutes between each step ...
-
bioRxiv - Developmental Biology 2024Quote: ... and Qubit (Invitrogen).
-
bioRxiv - Developmental Biology 2024Quote: ... Slides were mounted in Fluoromount G mounting media (Cat. No. 00-4958-02, Invitrogen) and imaged on a Leica THUNDER imager ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were then stained with Vybrant DyeCycle 1:500 (ThermoFisher, #V35004) for 1 hour at room temperature.
-
bioRxiv - Developmental Biology 2024Quote: ... or DAPI (Invitrogen).
-
bioRxiv - Developmental Biology 2024Quote: ... and Neurobasal medium (Invitrogen, 10888-022), supplemented with 0.5x B27 without vitamin A (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... MEFs were cultured on 0.1% gelatin in MEF medium consisting of DMEM GlutaMAX (Invitrogen, 61965-026), 10% FBS (Sigma-Aldrich F7524) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Feeder-depleted serum cultured ESCs were plated on gelatin-coated plates at 10,000 cells/cm2 (90,000 cells per well in a 6-well plate) in advanced N2B27 defined media composed of a 1:1 mix of 1:1 DMEM/F12 (Invitrogen, 12634-010) and Neurobasal medium (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... All lines were passaged using 0.25% Trypsin-EDTA (Invitrogen, 25200072). For this study we used 3 previously established littermate pairs of male Tet1-/- and Tet1+/+ B6129S6F1-Tet1tm1Koh ESC lines derived from mouse blastocysts ...
-
bioRxiv - Developmental Biology 2024Quote: ... 40 mg/ml BSA fraction V (Invitrogen, 15260037), 0.1 mM 2-mercaptoethanol (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... supplemented with 0.5x B27 without vitamin A (Invitrogen, 12587-010), 0.5x N2 (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... in Opti-MEM (Invitrogen, 31985070). One day after transfection ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.1 mM 2-mercaptoethanol (Invitrogen, 31350-010) and 100 U ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 100 U: 100 µg/ml penicillin: streptomycin (Sigma-Aldrich, P4333; or Gibco, 15140122). The medium was changed every day during differentiation ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.5x N2 (Invitrogen, 17502-048), 2 mM L-glutamine (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... PlatinumTM Taq DNA polymerase High Fidelity (Invitrogen, 11304-011) and PCR kit buffers ...
-
bioRxiv - Developmental Biology 2024Quote: ... and the Qubit™ dsDNA HS Assay kit (Invitrogen, Q32854). Amplicon pools were converted into sequencing libraries by performing a secondary PCR to incorporate indexes and sequencing adapters ...
-
bioRxiv - Developmental Biology 2024Quote: ... PCR products were gel-extracted using the PureLink Quick Gel Extraction kit (Invitrogen, K210012). Amplicon concentrations were quantified with the QubitTM dsDNA HS Assay kit (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... Amplicon concentrations were quantified with the QubitTM dsDNA HS Assay kit (Invitrogen, Q32854) and diluted to 15 nM.
-
bioRxiv - Developmental Biology 2024Quote: ... genomic DNA (gDNA) was extracted using the Purelink genomic DNA Mini Kit (Invitrogen, K182001) from E11.5 brains ...
-
bioRxiv - Developmental Biology 2024Quote: ... The gDNA was quantified using the Qubit 2.0 DNA HS Assay (ThermoFisher, Massachusetts, USA) and its quality was assessed with the Tapestation genomic DNA Assay (Agilent Technologies ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5-ethynyl-2′-deoxyuridine (EdU, 0.5 mg/ml in PBS; Thermo Fisher Scientific) was administered by intraperitoneal injection at 0.1 mg per 20 g body weight per injection ...
-
bioRxiv - Developmental Biology 2024Quote: ... in Ham’s F-10 medium with 1% penicillin/streptomycin (P/S, Gibco)) for 1.5h followed by centrifugation and washing ...
-
bioRxiv - Developmental Biology 2024Quote: ... in wash medium (10% Horse Serum (HS, Invitrogen)) in Ham’s F-10 medium with 1% penicillin/streptomycin (P/S ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated with appropriate AlexaFluor-conjugated secondary antibodies (1:2000, Invitrogen) for 1 h at RT ...
-
bioRxiv - Developmental Biology 2024Quote: ... These cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Wako) containing 10% FBS (Gibco or NICHIREI) and 1% penicillin-streptomycin (Wako ...
-
bioRxiv - Developmental Biology 2024Quote: ... and the heads were cut off and cultured in DMEM Ham’s/F’12 (Wako) supplemented with 1% N2 (Gibco) and 1% penicillin-streptomycin (Wako ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were incubated with the specific Alexa Fluor secondary antibody (1:500; Thermo Fisher) in 0.2% BSA in PBS for 1 hour at RT ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were removed under a microscope and immersed in ice-cold Hank’s Buffered Salt Solution (HBSS; #14175-095, Life Technologies). Meninges were gently removed ...
-
bioRxiv - Neuroscience 2024Quote: ... Total RNA was isolated from the biceps femoris using TRIzol (Invitrogen, Carlsbad, CA). The RNA samples were submitted to CapitalBio (https://www.capitalbiotech.com ...
-
bioRxiv - Neuroscience 2024Quote: ... The resulting cDNA was used as a template with SYBR Green Master Mix (Applied Biosystems) with designated primers (Table 1) ...
-
bioRxiv - Cell Biology 2024Quote: Protein samples were loaded on NuPAGE pre-cast polyacrylamide gels (Thermofisher Scientific) for SDS-PAGE electrophoresis ...
-
bioRxiv - Cell Biology 2024Quote: ... Pageruler (and Pageruler plus) prestained protein ladders (Thermofisher scientific Cat. No. 26616 and 26619) were used as protein molecular weight standards ...
-
bioRxiv - Cell Biology 2024Quote: ... the beads were resuspended in 4X LDS sample buffer (Invitrogen Cat. No. NP0007) containing 2% of 2-mercaptoethanol (Thermo scientific Cat ...
-
bioRxiv - Cell Biology 2024Quote: ... containing 2% of 2-mercaptoethanol (Thermo scientific Cat. No: 125470100) and heated at 70°C for 10 mins before freezing them at – 80°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were washed 8-10 times in PBS for 5 minutes and incubated with DAPI (0.1 mg/ml in PBS; #62248, Invitrogen) to stain nuclei ...
-
bioRxiv - Neuroscience 2024Quote: ... Neurons were seeded in neuronal medium [Neurobasal (#21103049, Gibco), 2% B27 (#A3582801 ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissues were incubated with 0.25% trypsin/EDTA (# 25200-056, Life Technologies) for 7 min at 37°C and the digestion was blocked with 10% FBS (#10500064 ...
-
bioRxiv - Neuroscience 2024Quote: ... for 7 min at 37°C and the digestion was blocked with 10% FBS (#10500064, Gibco) in DMEM (#41966-029 ...