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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were washed three times for 5 minutes in PBS and incubated for 1 hour in blocking solution [10% normal goat serum (NGS; #50197Z, Invitrogen), 0.1% Triton X-100 in PBS] ...
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bioRxiv - Neuroscience 2024Quote: The 24 TMT labeled proteome fractions were analyzed by LC/MS/MS using an Easy nLC 1200-Orbitrap Fusion Lumos (Thermo Scientific). Samples were injected onto an Easy Spray PepMap C18 column (75 μm id × 25 cm ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: anti-GFP (1:500; #A10262, Invitrogen), anti-GFAP (clone GA5 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μL Power SYBR green PCR Master Mix (Applied Biosystems # 4309155), 2 μl dH2O and 2 μL cDNA sample ...
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bioRxiv - Cell Biology 2024Quote: Primary human fibroblasts with or without mutant mitochondrial DNA (m.3243G) were routinely maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) containing 25 mM glucose ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with TO-PRO™-3 Iodide (Invitrogen, UK) for 15 min and mounted using ProLong Glass Antifade Mountant (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM (#41966-029, Life Technologies). Cortices were then mechanically dissociated by pipetting in DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... for 15 min and mounted using ProLong Glass Antifade Mountant (Thermo Fisher, UK) using high precision coverslips (0.170 ± 0.01 mm thick ...
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bioRxiv - Cell Biology 2024Quote: ... GlutaMAX (Gibco) and 0.1% or 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ml MEM vitamin solution 100x (ThermoFisher, ref.no. 11120037), 14 μl β-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ml MEM non-essential amino acids solution 100x (ThermoFisher, ref.no. 11140035), 1.63 g L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was determined by a BCA protein assay (Thermo Fisher Scientific Inc., Waltham, MA). Then ...
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bioRxiv - Neuroscience 2024Quote: A total of 16 samples (50 μg each) were labeled with TMT- pro reagents (Thermo Fisher) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... desmin (mouse monoclonal, MA5-15306, Invitrogen, CA), α-actin (rabbit polyclonal ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were injected onto an Easy Spray PepMap C18 column (75 μm id × 25 cm, 2 μm particle size) (Thermo Scientific) and separated over a 120 min method ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were incubated in stripping buffer (Thermo Scientific, Waltham, MA) for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-CAMKII alpha (ThermoFisher, MA1-048), rat anti-GAPDH (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were cut in the cutting solution using a vibratome (VT1000S; Leica Microsystems or Microm; Fisher Scientific), and transferred to a heated (37.5°C ...
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bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine 2000 (Invitrogen), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... IMR90 ER:RAS cells has been transiently transfected with 500ng of sin-lncRNA or empty pcDNA3.1 plasmid using Lipofectamine 2000 (Invitrogen) in opti-MEM medium (GIBCO) ...
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bioRxiv - Cell Biology 2024Quote: ... in opti-MEM medium (GIBCO). Primers employed for cloning are described in Table 4.
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bioRxiv - Cell Biology 2024Quote: ... in opti-MEM medium (GIBCO), unless differently stated in the text ...
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bioRxiv - Cell Biology 2024Quote: ... Beatriz Tavira (CIMA) and cultured in DMEM medium (GIBCO), supplemented with 10% fetal bovine serum (GIBCO ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected once with a final concentration of 40nM siRNA using Lipofectamine 2000 (Invitrogen) in opti-MEM medium (GIBCO) ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal bovine serum (GIBCO) and 1x penicillin/streptomycin (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: ... Dynabeads MyOne Streptavidin T1 beads (Invitrogen) were used to isolate RNA-protein complexes ...
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bioRxiv - Cell Biology 2024Quote: ... F-actin was stained with AF-488 phalloidin (1 drop in 4 ml FACS buffer; Invitrogen, Cat. # R37110) for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclear staining was performed using Hoechst 33342 (ThermoFisher, Cat ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Microarray data were analyzed using the Affymetrix Transcriptome Analysis Console 4.0 (TAC 4.0, Affymetrix) to identify differentially expressed genes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and protein concentration was determined by BCA protein assay kit (Thermo Fisher, 23225). Cells were lysed in the same RIPA buffer with protease and phosphatase inhibitors ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or Alexa Fluor 594-labeled donkey anti-sheep IgG (1:200; A11016, Life Technologies) for 2h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... incubated for ∼30 seconds with enhanced chemiluminescent substrate for HRP (Thermo Fisher, 34094 or 34075), and imaged using a digital imager ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and TraceMetal grade 70% HNO3 (ThermoFisher Scientific, Waltham, MA) were used as obtained ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Metabolites were measured on an orbitrap mass spectrometer (Q Exactive Plus, Exploris 240, or Exploris 480) coupled to a Vanquish UHPLC system (Thermo Fisher Scientific) with electrospray ionization and scan range m/z between 60-1000 at 1 Hz ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Equilibrium dialysis was performed using Rapid Equilibrium Dialysis kits (ThermoFisher Scientific 90006) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then Clariom S Assay was performed according to the manufacturer’s instruction (Affymetrix).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 17,18 The process involved homogenizing 25uL of 10 mg/ml brain tissues with ProcartaPlex™ Cell Lysis Buffer (ThermoFisher) and 5-mm stainless steel beads (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... and quantified by Qubit 3.0 using the dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA). DNA samples were stored at 4°C until used.
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bioRxiv - Genetics 2024Quote: ... Tol2 transposase mRNA was in vitro transcribed using the mMESSAGE mMACHINE T3 Transcription kit (ThermoFisher Scientific). For injections ...
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bioRxiv - Genetics 2024Quote: ... Purified DNA was qualified by Nanodrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and quantified by Qubit 3.0 using the dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Genetics 2024Quote: ... Streptavidin agarose beads (ThermoFisher Scientific) were washed three times in cold binding buffer (50mM Tris pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were placed in HI buffer containing 1:1,000 CellMask Deep Red (Invitrogen) for 15 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... coli (Cat.#C404010; Invitrogen), which underwent selection with Kanamycin ...
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bioRxiv - Developmental Biology 2024Quote: ... 10ng of total RNA was reverse-transcribed in a 10μL reaction using the SuperScript VILO cDNA Synthesis Kit (Invitrogen). The resulting cDNA was diluted to either 1:5 (mCherry ...
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bioRxiv - Developmental Biology 2024Quote: ... The gDNA in the RNA sample was digested by ezDNase and the cDNA was synthesized from 10ng of total RNA using SuperScript IV VILO Master Mix with ezDNase Enzyme kit (Thermofisher Scientifics). The cDNA libraries were constructed by Ion Ampliseq Library Kit Plus ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were dissociated with TrypLE Express Enzyme(ThermoFisher, 12605010) and were counted by hemocytometer.
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bioRxiv - Developmental Biology 2024Quote: ... Barcoded libraries were quantified using the Ion Library TaqMan Quantitation Kit (ThermoFisher Scientific Inc) and diluted to a final concentration of 80pM ...
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bioRxiv - Developmental Biology 2024Quote: ... Torrent Suite version 5.18.1 (Thermo Fisher Scientific Inc) was used to separate barcoded reads and to filter and remove polyclonal and low-quality reads ...
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bioRxiv - Developmental Biology 2024Quote: The targeted transcriptome sequencing was performed on the Ion S5XL Next Generation Sequencing system with the Ion AmpliSeq Transcriptome Human Gene Expression Assay (ThermoFisher Scientifics Inc). This assay covers 20,802 human RefSeq genes (>95% of UCSC refGene ...