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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... USA) equipped with an Acclaim 120 C18 HPLC column (4.6 x 150 mm, 5 μm; Thermo Fisher Scientific, Waltham, MA, USA). Solvent A was water with 0.1% formic acid and Solvent B was acetonitrile ...
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bioRxiv - Plant Biology 2024Quote: ... melongena PI 555598 acylsugar extracts were dried down in vacuo with centrifugation (Savant, ThermoFisher Scientific). Dried acylsugar extracts were dissolved in 1 mL of methanol ...
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bioRxiv - Plant Biology 2024Quote: ... We synthesized cDNA using 10 ng of RNA and SuperScript III Reverse Transcriptase (Invitrogen, Waltham, MA, USA). gDNA was isolated with the DNeasy Plant Mini Kit (Qiagen ...
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bioRxiv - Systems Biology 2024Quote: ... 0.2 U/µL Exonuclease I (Thermo Scientific, EN0581), 0.2 µM RCA primer and 0.2 µg/µL of BSA in Nuclease-free water overnight at 30°C.
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bioRxiv - Physiology 2024Quote: ... and 100 mg·ml−1 streptomycin (Gibco) at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with 5% goat serum (Thermofisher) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20% dialyzed fetal bovine serum (FBS, Life Technologies, #26400044), 1% penicillin streptomycin (P/S ...
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bioRxiv - Cancer Biology 2024Quote: ... PDX cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM, Life Technologies, #12440061), supplemented with 20% dialyzed fetal bovine serum (FBS ...
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bioRxiv - Physiology 2024Quote: ... AM (Molecular Probes) and 2 mM glucose ...
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bioRxiv - Physiology 2024Quote: RNA was extracted from the liver using the PureLink RNA mini kit (Invitrogen, 12183025) with DNase (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... respectively were stained with EdU (5-ethynyl-2′-deoxyuridine; Thermo Fisher) and modified pseudo-Schiff propidium iodide (PI ...
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bioRxiv - Physiology 2024Quote: ... The concentration and purity of RNA was determined using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA) and RNA was diluted to 100-400 ng/mL for sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... and cloned into pDONR/Zeo vector (Thermo Fisher) using Gateway Recombination Cloning Technology ...
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bioRxiv - Plant Biology 2024Quote: ... and cloned into pDONR/Zeo vector (Thermo Fisher) using Gateway Recombination Cloning Technology (Reece-Hoyes and Walhout ...
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bioRxiv - Physiology 2024Quote: ... with DNase (Invitrogen, 12185010) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Five-micron-thick cross-sections were made with the aid of a Microm HM 340E rotary microtome (Thermo Fisher Scientific Inc. ...
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bioRxiv - Physiology 2024Quote: ... Liver lysate was homogenized in RIPA buffer with EDTA-free Protease and Phosphotase Inhibitor Mini Tablet (Thermo Scientific, A32961). Each blot was normalized to its control sample or the Aged Control group ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription was performed using a SuperScript™ IV First-Strand Synthesis System (Catalog no. 18091050, Invitrogen, Waltham, MA, USA). 20 ng cDNA was used for real-time qPCR reactions with KAPA SYBR FAST qPCR Kit (Catalog no ...
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bioRxiv - Plant Biology 2024Quote: ... A NanoDrop ND1000 spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) was used for RNA quantification ...
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bioRxiv - Plant Biology 2024Quote: ... RT‒qPCR was performed on a QuantStudio 6 Pro (Applied Biosystems, Waltham, MA, USA) using GoTaq® qPCR Master MixA6001 (Promega ...
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bioRxiv - Physiology 2024Quote: ... or 0.75 UI human recombinant insulin/kg body weight for ITTs (catalog no. 12585014, Gibco™). Tail glucose measurements were then taken at the indicated time points in the figures to measure glucose clearance.
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bioRxiv - Plant Biology 2024Quote: ... All qPCR experiments were performed using two technical and three biological replicates and analyzed with Design and Analysis Software 2.6.0 (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Developmental Biology 2024Quote: ... First-strand cDNA was generated from pooled zebrafish RNA isolated from different developmental stages using SuperScript III first-strand synthesis kit (18080051, Invitrogen). Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... mRNA was synthesised using the mMESSAGE mMACHINETM SP6 Transcription Kit (ThermoFisher) according to the manufacturer’s specifications ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1µl SUPERase in RNAase inhibitor (ThermoFisher). Reaction was incubated for 4h at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... media (K562 and Jurkat cells) or DMEM (Gibco, 10569010) (HEK293T and HepG2 cells) ...
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bioRxiv - Synthetic Biology 2024Quote: ... in either RPMI 1640 (Gibco, 61870036) media (K562 and Jurkat cells ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected using the Neon™ Transfection System Kit (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: Using the NeonTM Transfection System Kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 0.25 mM each dNTP (Thermo Scientific, R0192), 0.2 U/µL Exonuclease I (Thermo Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... 10 µM ATP (Invitrogen, 18330019) and 0.2 µg/µL of BSA in Nuclease-free water.
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bioRxiv - Synthetic Biology 2024Quote: ... which was then immediately placed into a numbered PCR reaction (Platinum™ Green Hot Start PCR 2X Master Mix-Invitrogen). The sex-specific banding pattern following gel electrophoresis was evaluated for each egg to determine its sex ...
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bioRxiv - Systems Biology 2024Quote: ... The detection of protein synthesis was performed according to the protocol provided by the Click-iT® Plus OPP Protein Synthesis Assay Kits (Thermo Fisher, C10456). In brief ...
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bioRxiv - Synthetic Biology 2024Quote: ... Quantitative PCR was performed on a QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems). Relative gene quantification was performed using TaqMan primer probe set for LDLR (Hs01092524_m1 ...
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bioRxiv - Synthetic Biology 2024Quote: ... EdU detection was by Click chemistry-based AlexaFluor-488 flow cytometry assay (ThermoFisher). For RT-qPCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... using the Applied Biosystems TaqMan Fast Advanced Master Mix (Applied Biosystems, 4444963). Per qPCR reaction ...
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bioRxiv - Plant Biology 2024Quote: ... The products of this reaction were transformed into DH5α cells (Invitrogen) according to the manufacturer’s guidelines and grown on LB agar plates containing gentamycin (10 μg/ml ...
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bioRxiv - Plant Biology 2024Quote: ... Library concentration was assessed using a Qubit 2.0 fluorometer (Life Technologies) and the strand sizes were verified using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Synthetic Biology 2024Quote: ... Complementary DNA was synthesized from 500 μg of total RNA using the Maxima™ H Minus cDNA Synthesis Master Mix (Thermo Fisher Scientific). Quantitative PCR was performed on a QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (GIBCO), supplemented with 10% fetal bovine serum (Rocky Mountain Biologicals ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting PCR products were purified using a Qiagen QIAquick PCR purification kit and introduced in the gateway entry vector pDONR207 (Life Technologies) by BP clonase reactions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Relative gene quantification was performed using TaqMan primer probe set for LDLR (Hs01092524_m1) normalized against RPL19 (Hs02338565_gH) (Thermo Fisher). Each PCR reaction mix consisted of 0.5 μL primer probe ...
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bioRxiv - Synthetic Biology 2024Quote: ... bulk RNA was converted to cDNA through Maxima H Minus cDNA Synthesis reactions (Thermo Scientific, M1682). Afterwards ...
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bioRxiv - Synthetic Biology 2024Quote: ... Total RNA was extracted from the homogenate using the PureLink RNA Mini kit (Thermo Fisher Scientific). Complementary DNA was synthesized from 500 μg of total RNA using the Maxima™ H Minus cDNA Synthesis Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL-21 cDNA transcript sequences were amplified by TaqMan probe based qPCR reactions (ThermoFisher, 4331182) using the Applied Biosystems TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell pellets were flash-frozen and stored in -80℃ before processing for cell lysis and analysis by Cells-to-CT 1-Step TaqMan Kit with Taqman probes (ThermoFisher). For Western blots ...
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bioRxiv - Plant Biology 2024Quote: ... then placed on Gamborg’s B5 medium (Gibco) with the appropriate antibiotics (when used ...
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bioRxiv - Synthetic Biology 2024Quote: ... and afterwards specific cDNA transcript sequences of the above interleukin gene panel amplified by TaqMan probe based qPCR reactions (ThermoFisher, 4331182). Per qPCR reaction ...
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bioRxiv - Plant Biology 2024Quote: ... proteins were eluted by incubation with 4x LDS sample loading buffer (Invitrogen, CA, USA) at 95℃ for 3 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... 5 μL of TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific) and 1 μL of cDNA ...