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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 50 μL PCR reactions were prepared as follows: 10 μL GC buffer (ThermoFisher Scientific), 1 μL 10 mM dNTP mix (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA (cDNA) was generated using High-Capacity Reverse Transcription Kit (Thermo Fisher Scientific). cDNA was then used for quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... the pAAVS1-P-CAG-mNG21-10 repair template was purified prior to nucleofection using the GeneJET plasmid maxiprep kit (Thermo Fisher Scientific). Synthetic sgRNAs were purchased from MilliporeSigma and Synthego (listed in Table S3 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were HRP-coupled secondary antibodies (Invitrogen) in a dilution of 1:10000 and Clarity Western ECL Substrate was used to visualize the signal ...
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bioRxiv - Cell Biology 2024Quote: ... 14.5 μL of the reaction mix was loaded onto dPCR chips using the QuantStudio 3D Digital PCR 20K Chip Kit V2 (Thermo Fisher Scientific), taken through PCR thermocycling and analyzed on the QuantStudio 3D system (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 750 ng of plasmid purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Data was analyzed using the QuantStudio 3D AnalysisSuite Cloud Software (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... The final library concentration was measured using a Qubit fluorometer (Invitrogen) using the Qubit dsDNA HS assay kit (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5 ml of PRMI 1640 (Thermo Scientific) with 10 % FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Live-cell imaging was carried out in FluoroBrite DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: Isolated CDX2mCherry cells (n=5) mCherry-negative cells (n=4) and hES cells (n=2 pooled) were centrifuged and 200uL Tri reagent (Thermo Fisher Scientific, USA) was added and incubated at room temperature for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were mounted using Pro-long glass (Invitrogen). Preparations were maintained 24-48h in the dark at RT and then stored at 4°C up to image acquisition.
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 100 ng of RNA using the Superscript First Strand Synthesis System (Invitrogen). Gene products were analyzed by qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... using SYBR Green Master Mix (Invitrogen) and specific oligonucleotides in a QuantStudio 5 machine (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... 1× Antibiotic-Antimycotic solution (Invitrogen), and 20% L-929 conditioned medium for six days ...
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bioRxiv - Cell Biology 2024Quote: ... and specific oligonucleotides in a QuantStudio 5 machine (Applied Biosystems). To determine the H1 variants expression contribution to total mRNA H1 ...
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bioRxiv - Cell Biology 2024Quote: ... The mixed bone marrow cells were cultured in DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% Fetal Bovine Serum (Invitrogen), 1× Antibiotic-Antimycotic solution (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then washed three times 5 minutes with wash buffer and then dipped in dH2O before mounting with ProLong Gold Antifade Reagent (Thermo Fisher Scientific). Mounted samples were let to harden overnight at RT and then stored at 4°C until imaging.
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined by Micro BCA protein assay (Thermo Scientific) and immunoblot was performed.
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bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR analysis was performed using the StepOne Plus Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) or CFX Duet Real-Time PCR System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... anti-H1.5 (Invitrogen, 711912) and anti-H1X (abcam ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% Fetal Bovine Serum (FBS) (Invitrogen) and 1× Antibiotic-Antimycotic solution (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... bone marrow-derived macrophages (BMDMs) were seeded into six tissue culture-treated well plates at varying densities and cultured in RPMI-1640 medium (Invitrogen) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and GlutaMAX (Gibco). For live-cell imaging experiments a microscope incubator (Okolab ...
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bioRxiv - Cell Biology 2024Quote: ... and 1× Antibiotic-Antimycotic solution (Invitrogen) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The following conjugated secondary antibodies (Invitrogen) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Hoechst 33342 (Invitrogen) was added to the cells at a final concentration of 1.78 μM (1 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... The pellets were resuspended in cold lysis buffer (Table EV5) supplemented with protease and phosphatase inhibitors (2 mM Na3VO4, 1 tab/10 ml; Thermo Fisher Scientific). Cell suspensions were lysed using mechanical shearing by pipetting and then sonicated (10-15 pulses at 50% power) ...
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bioRxiv - Physiology 2024Quote: ... and 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco, 15140-122). To induce differentiation ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated with secondary antibody (1:400, Alexa Fluor 488 goat anti-mouse, Invitrogen, a11001) for two hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... containing 4.5 g/l glucose and 10% fetal bovine serum (Thermo Fisher Scientific, USA, #10438026). To synchronize cells in G0 by S.S. ...
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bioRxiv - Cell Biology 2024Quote: ... 30 μl of Dynabeads (Thermo Fisher Scientific, MA, USA, #10004D) were added and incubation was continued for an additional 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
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bioRxiv - Cell Biology 2024Quote: ... in accordance with manufacturer’s instructions (DyLightTM, Thermo Fisher Scientific). For chemiluminescent western blotting ...
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bioRxiv - Cell Biology 2024Quote: ... followed by application of secondary antibody goat anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific, MA, USA, #A21202, 1:200) or donkey anti-rabbit Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... On the day of the experiment, cells were washed (three times, PBS, 37°C) and serum starved in EBSS (Gibco) for 2 h prior to insulin stimulation ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then visualized using secondary antibody conjugated to Alexa Fluor-488 (Thermo Fisher Scientific, MA, USA, #A21202) or Alexa Fluor-594 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were quantified using a BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA, #23250). One milligram of total protein was incubated with anti-Mecp2 (5 μg ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR reactions were performed using an ABI 7500 system (Applied Biosystems, Foster City, CA, USA). Samples were normalized for expression levels of human or mouse actin ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded on #1.5 glass coverslips (Thermo Fisher Scientific) in dishes and experimental procedures (transfection ...
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bioRxiv - Cell Biology 2024Quote: ... The molecular migration position of transferred proteins was compared with the PageRuler Prestain Protein Ladder 10– 180 kDa (Themo Fisher Scientific). Signal quantification was performed using Fiji ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were counterstained using DAPI (Thermo Fisher Scientific, MA, USA, #D-1306). Following a wash in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The cellular lysates of G0 and cell cycle re-entry-cells were lysed in IP lysis buffer (Thermo Fisher Scientific, MA, USA, #87787), and the supernatant was obtained by centrifugation at 10000 × g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 10 µg of chromatin was diluted in ChIP buffer and pre-cleared with Dynabeads Protein G (Invitrogen, 10004D) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The following sequences (Invitrogen, Stealth) were used ...
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bioRxiv - Cell Biology 2024Quote: ... and Penicillin/Streptomycin (Life Technologies). Jurkat E6.1 cells were maintained with RPMI1640 (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... 50% Neurobasal medium (Gibco), B-27 minus Vitamin A (Thermo Fisher 12587010) ...
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bioRxiv - Cell Biology 2024Quote: ... ROS levels were detected using CellROX (C10422, Life Technologies) by flow cytometry ...