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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... All cells were grown in DMEM (4.5 g/L glucose, Gibco) supplemented with 5% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with H2DCFDA (Invitrogen, 20 uM) and Hoechst 33342 (Thermo Fisher ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: The cDNA of MALAT1 or CDC42 was cloned into pcDNA3.1 vector (Invitrogen, Thermo Fisher Scientific). An empty pcDNA3.1 vector was used as a negative control (NC) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% penicillin/streptomycin (Gibco) with 5% CO2 at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and Hoechst 33342 (Thermo Fisher) for 30 min in the dark at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... the aqueous phase was collected and combined with isopropanol and 1.5 µg of GlycoBlue coprecipitant (Invitrogen). After a 10-minute incubation at room temperature ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... The bone marrow cavity was repeatedly blown with DMEM (Gibco, Grand Island, NY, USA) low sugar medium ...
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bioRxiv - Microbiology 2024Quote: ... The concentrations of RNA and cDNA were determined using a Qubit 4 fluorometer (Thermo Fisher Scientific, USA).
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bioRxiv - Systems Biology 2024Quote: OCT-embedded retinal and RB samples were cryosectioned to 20 μm sections using a CryoStar NX70 (Thermo Fisher Scientific), and placed onto the capture areas of the Visium Spatial Tissue Optimisation and Visium Gene Expression slides following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 5 % CO2 and 95 % rH in Iscove’s Modified Dulbecco’s Medium (Gibco), supplemented with 20 % (v/v ...
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bioRxiv - Systems Biology 2024Quote: DISPA of the plasma proteome peptides was performed on an Orbitrap Fusion Lumos™ mass spectrometer (Thermo Fisher Scientific) coupled with the FAIMS Pro Interface ...
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bioRxiv - Systems Biology 2024Quote: ... coupled on-line to an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... 2 mM L-glutamine,1% (v/v) penicillin/streptomycin(Gibco)) and 2 million cells were seeded in 6cm collagen I-coated dish (BD BioCoat) ...
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bioRxiv - Systems Biology 2024Quote: ... The Ultimate™ 3000 HPLC system (Thermo Fisher Scientific, USA) was used to control the automated sample loading ...
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bioRxiv - Systems Biology 2024Quote: ... The sample was first loaded on a PepMap C18 trap-column (75 µm ID x 50 mm length, 3 μm particle size, 100 Å pore size, Thermo Fisher Scientific) before reversed-phase separation on a 50 cm analytical column (in-house packed with Poroshell 120 EC-C18 ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 10% (v/v) FCS (Gibco),0.1μM dexamethasone ...
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bioRxiv - Systems Biology 2024Quote: ... Protein concentration of the lysates was determined by application of the BCA Protein Assay Kit (Pierce, Thermo Scientific). 20 µg protein per sample was used for further sample preparation ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: BM-MSCs in logarithmic growth phase and in good condition were washed twice with PBS (Gibco, Grand Island, NY, USA). Subsequently ...
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bioRxiv - Systems Biology 2024Quote: ... Peptide mixtures were analyzed by orbitrap mass spectrometer Q Exactive Plus (Thermo Scientific) connected to an electrospray ion source ...
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bioRxiv - Systems Biology 2024Quote: ... peptide mixtures were separated by a reversed-phase chromatography (Dionex Ultimate 3000 NCS-3500RS Nano, Thermo Fisher Scientific) coupled on-line to an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and constructs were then transfected into tsA201 cells with Lipofectamine 2000 (Invitrogen). After transfection ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were visualized with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, catalog # 34577) using the ChemiDoc™ XRS+ System.
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bioRxiv - Zoology 2024Quote: ... Photographs of cross-sections set at the symphyseal midline were acquired using Avizo v.7.0 (Thermo Fisher Scientific, Waltham). First ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCR was performed using the QuantStudio 6 Flex real-time PCR system (Applied Biosystems, Waltham, MA). The thermal cycling conditions were as follows per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined by BCA kit (23228, Thermo Scientific). 20 ugm of protein was loaded and separated on NuPAGE 4 to 12% ...
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bioRxiv - Neuroscience 2024Quote: ... added with protease and phosphatase inhibitor (78440, Thermo Scientific). Protein concentration was determined by BCA kit (23228 ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs were extracted from the L3 and L4 DRG tissues using TRIzol (Thermo Fisher Scientific, #15596026). The RNA concentration was quantified using a NanoDrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Zoology 2024Quote: ... PCR were run using Phusion U Hot Start DNA Polymerase (Thermo Scientific) and indexed PCR primers ...
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bioRxiv - Zoology 2024Quote: ... China) was equipped with a TG-WaxMS capillary column (60 m × 0.25 mm × 0.25 µm, Thermo scientific) and a flame ionization detector (FID) ...
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bioRxiv - Neuroscience 2024Quote: ... The RNA concentration was quantified using a NanoDrop 1000 (Thermo Fisher Scientific). Good quality of RNA was confirmed using 260nm/280nm absorbance of 2 using a Nanodrop spectrophotometer and was then sent to the Advanced Technology Genomics Core at MD Anderson for RNA-sequencing ...
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bioRxiv - Neuroscience 2024Quote: One microgram of RNA was first treated with RNAase-free DNase and then used for reverse transcription with the SuperScript IV VILO Master Mix reverse transcription kit (11766050; Thermo Fisher Scientific). Two microliters of complementary DNA was diluted 5 times and added to a 20-µL reaction volume with SYBR Green real-time PCR mixture (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2024Quote: ... sgRNA templates were transcribed using a T7 promoter and synthesized in vitro using the MAXIscript T7 kit (Ambion, Austin, USA) according to the manufacture’s instruction ...
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bioRxiv - Zoology 2024Quote: ... Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, USA).
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bioRxiv - Neuroscience 2024Quote: Protein lysate was made from L3/L4 DRGs using RIPA (89900, Thermo Scientific) added with protease and phosphatase inhibitor (78440 ...
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bioRxiv - Neuroscience 2024Quote: ... under reducing condition in MES SDS running buffer (NP002, Thermo Scientific) as per manufacturer recommendation ...
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bioRxiv - Microbiology 2024Quote: Dissolved inorganic nutrients (NO3-+NO2-, PO43-) were measured by standard colorimetric methods (Grasshoff et al. 1999) using a photometric analyzer (Thermo Scientific Aquacem 250). NH4+ was measured with a Hitachi U-1100 spectrophotometer.
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bioRxiv - Microbiology 2024Quote: ... 500 μl of Phenol:Chloroform:IAA (25:24:1) (Ambion) was added to the wax tube ...
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bioRxiv - Microbiology 2024Quote: ... and concentrations of indexed cDNA were measured using the Qubit®dsDNA BR Assay Kit (Invitrogen, Carlsbad, CA, USA). Approximately 28 ng of DNA from each sample was added to a pool ...
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bioRxiv - Microbiology 2024Quote: ... cells were kept in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific) which was complemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... The resulting PCR product was subcloned into pCR™8TOPO™ vector (Invitrogen™) by TA cloning (84) ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into pENTR™/D-TOPO™ (Invitrogen™). The AS2 coding sequence was cloned into the pATML1 containing gateway vector pAR176 (Basta resistance (46) ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into pENTR™/D-TOPO™ vector (Invitrogen™). The resultant vector was LR recombined into the gateway vector pBGWFS7 (85) ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was then washed twice with 5 ml of ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen) before prehybridization with 10 ml of the same buffer at 42°C for 45 minutes.
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bioRxiv - Microbiology 2024Quote: ... For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142, Thermo Fisher, France) and revealed using an anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... was cultured in Minimum Essential Medium (MEM, Gibco; Invitrogen) containing 4% FCS and 1% L-Glutamine (L-Gln ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% foetal bovine serum (FBS, Gibco). Vero-E6 (ATCC® CRL-1586 ™) ...
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bioRxiv - Developmental Biology 2024Quote: Mouse liver tissue was weighed and homogenized in RIPA Lysis and Extraction Buffer (Thermo Scientific, #89900) with the Protease and phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The first strand cDNA was obtained using oligo(dT) primers and Moloney murine leukaemia virus-reverse transcriptase (Life Technologies), using 100 ng of purified RNA ...
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bioRxiv - Microbiology 2024Quote: ... DNA was digested using the TURBO DNase (Ambion), purified with phenol:chlorophorm:isopropanol ...
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bioRxiv - Molecular Biology 2024Quote: ... particles were eluted from the beads by adding 1X elution buffer made of Solution A (16 mM EDTA, UltraPure pH 8.0, ThermoFisher) and Solution B (10 mM NaCl ...