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Citations for Thermo Fisher :
2951 - 2985 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... b-galactosidase activity was measured using the Tropix Galacto-Light Plus™ chemiluminescent kit according to the manufacturer’s protocol (Applied Biosystems, Foster City, CA).
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bioRxiv - Bioengineering 2023Quote: The cDNA of either oFluc (RDB14359 from RIKEN BRC) or Venus/Akaluc 20 was inserted in the multiple cloning site of pRSET-B using BamHI and EcoRI (Thermo Fisher Scientific, MA, USA). The luciferase protein expressed in NiCo21(DE3 ...
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bioRxiv - Genomics 2023Quote: ... 5 U/mL, human Chorionic Gonadotropin-hCG (Chorulon®, Intervet International B. V., Boxmeer, Netherland) and 50 μg/mL gentamicin (Gibco Thermo Fisher, Massachusets - USA). Culture droplets were covered with mineral oil and placed in an incubator at 38.5°C and a humid atmosphere of 5% CO2 in atmospheric air ...
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bioRxiv - Neuroscience 2023Quote: ... To obtain a single cell suspension the cells were mechanically dissociated with a glass Pasteur in neuronal culture medium (Neurobasal™ Plus Medium, Gibco A3582901, 1X B-27™ Plus Supplement, Gibco, A35828-01); the cell concentration was calculated with the T20 automated Cell Counter (Biorad) ...
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bioRxiv - Immunology 2023Quote: ... from singly sorted antigen-specific memory B cells were RT-PCR amplified using SuperScript III and AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Waltham, MA) under conditions previously described46.
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bioRxiv - Neuroscience 2024Quote: ... Eyes were injected with a glass pipette intravitreally with 5µl of 0.2% cholera toxin B subunit (CTB) conjugated to Alexa Fluor 594 or 488 (Invitrogen by Life Technology, Carlsbad, CA, USA) diluted in 1% DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were plated at 20% confluence in complete DMEM supplemented with 150 μg/mL hygromycin B (Invitrogen, Thermo Fisher Scientific, Carlsbad CA, US). During selection ...
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bioRxiv - Microbiology 2020Quote: ... formic acid in LC-MS grade acetonitrile (solvent B) were used in conjunction with a Syncronis™ HILIC LC column (Thermo Fisher Scientific 97502-102130) to achieve chromatographic separation of chemical compounds ...
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bioRxiv - Immunology 2020Quote: ... cells were washed twice with FACS buffer and staining with antibodies was performed in Medium B permeabilization buffer (Thermo Fisher Scientific, Cat. No. GAS002S-100) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: All strains were cultured for 16-20 hours (B. anthracis) or 24 hours (Y. pestis) on BD BBLTM Trypticase Soy Agar II with 5% sheep blood (SBA) (Thermo Fisher Scientific, Waltham, MA, USA) at 35°C in ambient air from glycerol stocks stored at −170°C.
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bioRxiv - Neuroscience 2021Quote: ... 2 % B-27, [Gibco, 17504-044], Penicillin [200 U/mL]/Streptomycin [200 μg/mL] [Gibco, 15140-130], 2 mM GlutaMAX [Gibco, 35050-038] in Neurobasal-A), and incubated at 37 °C with 5 % CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-MGO-derived Advanced Glycation and Products (AGEs) (KAL-KH001 Cosmo-Bio, USA, 1:500 dilution) and normalized to ²-actin levels (anti-b-actin 1:5000; Thermo Fisher Scientific; Waltham, MA, USA).
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bioRxiv - Plant Biology 2022Quote: RT-qPCR analysis was performed using an Absolute Blue qPCR SYBR Green ROX Mix (AB-4162/B) kit (Thermo Fisher Scientific, Waltham, MA 15 USA). Reactions were performed using a Rotor-Gene 6000 cycler (Corbett Research ...
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bioRxiv - Neuroscience 2022Quote: ... consisting of DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml 1% penicillin/ streptomycin (pen/strep; all Thermo Fisher Scientific, Waltham, MA, USA) + 20 ng/ml BDNF + 20 ng/ml GDNF + 20 ng/ml NGFb (all Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... collected as single cell suspensions using cell strainer and resuspended in sphere formation medium which was composed of 1:1 DMEM and F-12 nutrient mix supplemented with B-27 and N-2 (Thermo Fisher Scientific, Waltham, MA, USA). 1,000 cells were plated in each well of ultra-low attachment 6-well plates (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... They were thawed and cultured according to the manufacturer’s recommendation in a B-27 Plus Neuronal Culture System consisting of Neurobasal Plus (NB Plus) medium and B27 Plus supplement (Thermo Fisher Scientific, cat. no. A3653401). Culturing medium was prepared by adding 2% of B27 Plus supplement and 1% of PS to Neurobasal Plus + (NB Plus +) ...
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bioRxiv - Neuroscience 2020Quote: ... On DIV3 the entire plating medium was replaced with 1 ml maintenance medium (49 ml Neurobasal-A, 1 ml B-27 supplement, 500 µl L-Glutamine, 50 µl Penicillin-Streptomycin; all Thermo Fisher Scientific Inc., Waltham, USA). On DIV11 ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed twice with FACS buffer and staining with antibodies was performed in Medium B permeabilization buffer (Thermo Fisher Scientific, Cat. No. GAS002S-100) for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: RT-qPCR analysis was performed using an Absolute Blue qPCR SYBR Green ROX Mix (AB-4162/B) kit (Thermo Fisher Scientific, Waltham, MA 15 USA). Reactions were performed using a Rotor-Gene 6000 cycler (Corbett Research ...
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Cell Biology 2024Quote: ... the remaining cells were then plated in in 96-well plates in B cell Medium (RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA, 21875034) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... 30 min 95% B at a flow rate of 300 nL/min using an UltiMate 3000 RSLCnano LC system (Thermo Fisher Scientific, Waltham, MA, USA). The elution from the column was analyzed using a Q Exactive HF-X (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... KSR medium was replaced by N2 medium (N2; DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml pen/strep [all: Thermo Fisher Scientific, Waltham, MA, USA]), supplemented with 2i + 3i in 25% increments every two days until day 10 ...
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bioRxiv - Genomics 2023Quote: ... 5 U/mL, human Chorionic Gonadotropin-hCG (Chorulon®, Intervet International B. V., Boxmeer, Netherland) and 50 μg/mL gentamicin (Gibco Thermo Fisher, Massachusets - USA). Culture droplets were covered with mineral oil and placed in an incubator at 38.5°C and a humid atmosphere of 5% CO2 in atmospheric air ...
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bioRxiv - Bioengineering 2023Quote: ... according to Pneumacult™ Ex-Plus (STEMCELL Technologies Inc., catalog #05040; supplemented with amphotericin B (0.25 μg/mL final concentration) (Thermo Fisher Scientific, catalog #BP264520), gentamicin (50 μg/mL final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... was injected into the soleus muscle and Alexa Fluor 488-conjugated Cholera Toxin subunit B (CTB 488, Thermo Fisher Scientific, 0.05% in 5 µL PBS) into the gastrocnemius muscle ...
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bioRxiv - Biophysics 2024Quote: ... The tissue pieces were then gently moved into 2 mL of neuronal culture medium (Neurobasal A, B-27 and GlutaMAX supplements, Thermo Fisher, with 1× penicillin-streptomycin) to dissociate the cells ...
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bioRxiv - Neuroscience 2024Quote: ... KSR medium was replaced in 25%-steps with N2 medium (N2; DMEM/F12 GlutaMAX, 1X B-27 Plus Supplement, 1X N-2 Supplement, 100 U/ml penicillin/streptomycin [all: Thermo Fisher Scientific, Waltham, MA, USA]) every other day ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed again and incubated for 10 minutes in Sudan Black B (0.1% (w/v) in 70% ethanol) to reduce autofluorescence before incubation with DAPI for 10 minutes (Thermo Scientific, #62248; 1:5000 dilution in PBS) to visualize cytoarchitecture ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 hMSCs from 4 donors chosen randomly (2 male; B, G, and 2 female; C, D) were thawed and cultured in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) supplemented with RoosterNourishTM-MSC expansion medium (RoosterBio ...
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B). Once the HepG2 or HC-04 cells were grown to the desired concentration ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... Protein extraction and determination was performed using the B-PER™ Bacterial Protein Extraction Reagent and Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively.
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bioRxiv - Immunology 2019Quote: ... were sorted into B cell media (IMDM medium, GIBCO; 10% heat-inactivated low IgG FBS, Life Technologies; 5 ml GlutaMAX, Life Technologies; 1 ml MycoZap plus PR, Lonza). Immediately following the sort ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156, 5 μg/mL puromycin, Life Technologies A11138-03, and 2 μg/mL doxycycline). On day three ...