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Citations for Thermo Fisher :
2601 - 2650 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... FACs sorted tonsil B cell subsets were seeded on to poly-L-lysine coated chamber slides (Nunc Lab-Tek, Thermo Scientific) stimulated ...
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bioRxiv - Immunology 2021Quote: ... FACs sorted tonsil B cell subsets were seeded on to poly-L-lysine coated chamber slides (Nunc Lab-Tek, Thermo Scientific) stimulated ...
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bioRxiv - Microbiology 2019Quote: ... Neural maintenance medium base (50% DMEM-F-12 medium-GlutaMAX, 50% Neurobasal-A medium, 1 × N-2 supplement, 1 × B-27 supplement [Life Technologies]) supplemented with 1% DMSO (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... at 30,000/well in neuronal culture medium (neural basal medium (NBM) containing 0.5 mM GlutaMax and B-27 supplement (Invitrogen; Carlsbad, CA).
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bioRxiv - Immunology 2019Quote: ... B cell regions near the surface of the LN were manually removed using the filament editor in Amira (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... to produce tandem mass spectra whose b-and y-ion series patterns were compared to theoretical digests of the proteome in Sequest (Thermo-Fisher Scientific) to establish protein identity ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Biophysics 2019Quote: ... chemically fixed CH27 B cells were incubated with anti-mouse CD45R (B220) primary antibody clone RA3-6B2 conjugated directly to Alexa 532 (Thermo Fisher Scientific Cat# 14-0452-81 ...
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bioRxiv - Cancer Biology 2019Quote: ... and buffer B (80 % acetonitrile, 0.1 % formic acid) was used for peptide separation on an Easy-nLC 1200 (Thermo Fisher Scientific). This system was coupled via a nano electrospray ionization source to the quadrupole-based Q Exactive HF benchtop mass spectrometer 43 ...
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bioRxiv - Microbiology 2021Quote: ... Salivary glands of mosquitoes were dissected 14-20 days post-blood meal in L15 medium supplemented with antibiotics and Amphotericin B (Gibco™).
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bioRxiv - Bioengineering 2021Quote: ... The isolation was carried out following the manufacturer’s instructions and B cells were isolated in phosphate-buffered saline (PBS) free from Ca++ and Mg++ (Gibco, Thermo Fisher Scientific). The B cells suspended in the PBS from the final incubation were centrifuged at 400g for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... B: 80% acetonitrile/0.1% formic acid in water on a 50 cm Acclaim PepMap RSLC C18 column (Thermo Fisher Scientific #164942) operated by a Dionex ultimate 3000 RSLC nano pump with column heating at 50°C connected to an Orbitrap Fusion Lumos ...
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bioRxiv - Neuroscience 2021Quote: ... A pulled glass needle was introduced in the vitreous through the ora serrate and 2μl of 2μg/μl Alexa Fluor 594-conjugated cholera toxin B (CTB) (Thermo Fisher Scientific, C34777) was injected ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies] ...
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bioRxiv - Neuroscience 2020Quote: ... Three hours after plating medium was exchanged for maintenance medium (Neurobasal-A without Phenol Red, 2% B-27 supplement, 1% penicillin-streptomycin, 0.5 mM glutaMAX, 25 μM β-mercaptoethanol; ThermoFisher Scientific) and cells were kept at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... B: 80% acetonitrile/0.1% formic acid in water on a 50 cm Acclaim PepMap RSLC C18 column (Thermo Fisher Scientific #164942) operated by a Dionex ultimate 3000 RSLC nano pump with column heating at 50°C connected to an Orbitrap Fusion Lumos ...
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bioRxiv - Bioengineering 2020Quote: Beads are 3 µm radius biotin-polystyrene microspheres (Kisker Biotech,PC-B-3.0) on which we attached Alexa Fluor (AF) 647 functionalized with streptavidin (Life Technologies, S21374). We prepared a solution containing 500 µL of water ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... except data for Figure 3a-c and Figure 4a-b were collected on an EVOS XL CORE Imaging microscope (ThermoFisher Scientific) using 20X ...
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bioRxiv - Cell Biology 2021Quote: ... Antibiotic-Antimycotic (100 units/mL of penicillin, 100 μg/mL of streptomycin and 250 ng/mL of amphotericin B, Gibco 15240062), and GlutaMax ...
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bioRxiv - Cell Biology 2021Quote: ... and were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% FCS and 100 μg/mL of hygromycin B (Invitrogen, 10687010).
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bioRxiv - Biochemistry 2020Quote: ... buffer B: 80% (v/v) acetonitrile + 0.1% (v/v) formic acid) to a quadrupole-orbitrap Q Exactive Plus (Thermo Fisher Scientific) MS/MS via a nano-electrospray source (positive mode ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stably transduced with retroviral vectors that encoding mCherry-GFP-LC3 and puromycin or hygromycin resistance genes for 24h and selected in 1 μg/mL puromycin or 100 μg/mL hygromycin B (ThermoFisher #10687010) for two or five days ...
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bioRxiv - Neuroscience 2020Quote: ... HD and corrected C116 iPSC-derived neural stem cells (NSCs) were plated and cultured in Neural Proliferation Medium (NPM) (Neurobasal medium, 1X B-27 supplement (Life Technologies), 2mM L-Glutamine ...
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bioRxiv - Neuroscience 2020Quote: ... Small craniotomies were made at these sites and cholera toxin subunit B (CTB) conjugated with Alexa Fluor (CTB-488, −555, −647, Thermo Fisher) was injected using glass micropipettes ...
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bioRxiv - Biophysics 2021Quote: ... Bacteria were pelleted by centrifugation and soluble cell lysates were extracted using Bacterial Protein Extraction Reagent (B-PER, Thermo Fisher Scientific). Lysates were then screened for photochromism ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized using ketamine / xylazine and intraviteally injected with 2 μl AF488 cholera toxin subunit B (1 mg/ml in PBS) (ThermoFisher Scientific). After 72 h mice were anaesthetized and euthanized via 4% PFA cardiac perfusion ...
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bioRxiv - Neuroscience 2020Quote: ... 0.2 μl of 1.5% cholera toxin subunit B conjugated with Alexa Fluor® 594 (CTB; Thermo Fisher Scientific, Waltham, MA, USA) were injected subcutaneously into the second and third digit of the left and right hind paw ...
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bioRxiv - Immunology 2019Quote: Splenic B-cells were purified and purity was confirmed by flow cytometry prior to labelling with Cell Trace Violet (CTV; Life technologies) as per manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... PMH were cultured in hepatocyte culture medium (William’s E medium, GlutaMAX™ Supplement, Gibco™ 32551020; 100 U/ml penicillin/streptomycin, Sigma P4333; 0.5 µg/ml Fungizone antimycotic B, Gibco 15290018 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and isWT7.22 (UMGi020-B clone 22) were generated from dermal fibroblasts using the integration-free CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), and characterized previously51 ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then lysed by resuspending the frozen pellet resulting from 82 mL of culture in 160 μL of B-PER II bacterial protein extraction reagent (Thermo Scientific). We then added 3.8 mL of 1x BMg/THS buffer and 0.8 μL of ReadyLyse lysozyme solution (Lucigen) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μM E64D) and incubated for 1 h in CCF2 solution (LB, CCF2-AM, Solution B [CCF2-AM kit K1032] Thermo Fisher) in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... 1299003– HSS120158 and siChe-1 b, cat. n. 1299003–HSS120159) or a control sequence (siControl, cat. n. 12935300) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cardiac cells were subsequently thawed in RPMI+B27 media (RPMI 1640 supplemented with 2% B-27 Supplement minus vitamin A, 1% GlutaMAX and 1% Penicillin/Streptomycin- All from ThermoFisher Scientific) with Rock inhibitor (Selleck Chemicals ...
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bioRxiv - Neuroscience 2022Quote: ... Mobile phases A and B were with 0.1% formic acid in water and 0.1% formic acid (Fisher Scientific; Cat#: A11710X1-AMP) in ACN (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... buffer B: 80% (v/v) acetonitrile + 0.1% (v/v) formic acid) to a quadrupole-orbitrap Q Exactive Plus (Thermo Fisher Scientific) MS/MS via a nano-electrospray source (positive mode ...
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bioRxiv - Molecular Biology 2022Quote: ... streptavidin particles with immobilized peptides were washed three times with buffer B (PBS pH 8.3, 5% glycerol, 1 mM DTT, 0.1% NP-40, Pierce™ Phosphatase Inhibitor Mini Tablets (Thermo Fisher Scientific), Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... Stable U-2 OS FT cell lines were maintained in the presence of 200 µg/mL hygromycin B (Gibco/Thermo Fisher) and 5 µg/mL blasticidin S ...
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bioRxiv - Molecular Biology 2022Quote: ... Stable U-2 OS FT cell lines were maintained in the presence of 200 µg/mL hygromycin B (Gibco/Thermo Fisher) and 5 µg/mL blasticidin S ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... RNA was isolated (EZNA HP Total RNA kit) and cDNA was synthesized (Thermo Scientific, Verso cDNA Synthesis Kit #AB-1453/B).
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bioRxiv - Cancer Biology 2022Quote: ... with 10% FBS and 1x antibiotic-antimycotic (100 units/mL penicillin, 100 ug/mL streptomycin, 0.25 ug/mL Amphotericin B; Gibco, cat. 15240096) in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: INS-1E cells (Merglen et al., 2004) (kind gift of B. Guigas, LUMC) were cultured in Roswell Park Memorial Institute (RPMI) medium 1640 (Gibco, Life Technologies) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... the culture medium was replaced with RPMI 1640/GlutaMAX medium supplemented with insulin-containing B-27 supplement (ThermoFisher Scientific; #17504-044). On day 11 ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were separated using a 180 minutes long gradient ranging from 3-15% solvent B (after optimization with pre-digested HeLa samples from Thermo Fisher). The mass spectrometer was run in positive mode with full MS (m/z = 400-2000 ...
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bioRxiv - Neuroscience 2021Quote: ... CHO-Q3 stable cells were maintained under dual selection with Zeocin (100 μg/ml) and hygromycin B (600 μg/ml) in F-12 nutrient mixture medium (GIBCO/Invitrogen) supplemented with 10% fetal bovine serum (ATLANTA Biologicals) ...
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bioRxiv - Immunology 2020Quote: Ramos B Cells at a concentration of 10 million cells/mL were incubated with 10 μM Fluo-4 AM (ThermoFisher, Inc.) for 30 minutes at 37C ...
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bioRxiv - Neuroscience 2021Quote: ... mice were anesthetized as described before and intravitreal injections of CTB-555 (Cholera toxin subunit B, Alexa Fluor 555-conjugated, ThermoFisher Scientific) were performed ...
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bioRxiv - Cancer Biology 2022Quote: ... except that some preparations were made using donor T cells that were activated for 48 hours before virus infection using LentiBoost (5 μl each of solutions A and B per ml of cells transfected; Fisher Scientific). 100 units/ml IL-2 was added at the start of the activation procedure and with every media change during the expansion phase.