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Citations for Thermo Fisher :
2901 - 2950 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: The 3’UTR of Aurora B was amplified with PCR using sea urchin cDNA and cloned into the ZeroBlunt vector (ThermoFisher Scientific, Waltham, MA) (Table 1 Primers) ...
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bioRxiv - Immunology 2022Quote: ... and stimulated in B cells complete medium (R10, RMPI 1640 10% FBS Hyclone complete medium) supplemented with ITS (1:100, Thermo Fisher, # 41400-045). To stimulate murine B cells ...
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bioRxiv - Molecular Biology 2022Quote: ... LC mobile phase solvents consisted of 0.1% formic acid in water (Buffer A) and 0.1% formic acid in 80% acetonitrile (Buffer B, Optima™ LC/MS, Fisher Scientific, Pittsburgh, PA). After 22 uL of sample loading at a maximum column pressure of 700 bar ...
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bioRxiv - Microbiology 2022Quote: ... or hygromycin B phosphotransferase gene (HYG) containing three or two stop codons respectively and TOPO cloned into a pCR4 backbone (Thermo Fisher, Waltham, Massachusetts). Linear homology-directed repair donor DNA was generated by PCR using ultramers (IDT ...
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Neuroscience 2022Quote: ... The injections were either a fluorescent dextran amine or a recombinant cholera toxin subunit B tracer: (Dextran-405; CTB-488, CTB-594, CTB-647, Invitrogen #C22841, #C34777, #C34778). We chose our injection volume based on the size ...
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bioRxiv - Cancer Biology 2022Quote: Expression levels of 754 miRNAs were quantified using the TaqMan Human MicroRNA Array A+B Card Set v3.0 (Applied Biosystems, Foster City, CA).
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bioRxiv - Molecular Biology 2024Quote: ... were amplified from differentiated P19 mouse embryonal carcinoma cells15 and ligated into the multiple cloning site of the expression vector pcDNA3.1 myc-His B (Thermo Fisher Scientific; Waltham, MA). Snapin was cloned in-frame with the downstream myc-His tags ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were selected for the integration of the transgene 48 h post-transfection with 250 µg/mL hygromycin B (Thermo Fisher Scientific, 10687010). Selection medium was refreshed every 2–3 days and the hygromycin-resistant cell population expanded ...
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bioRxiv - Cell Biology 2024Quote: ... 500 nM recombinant MEC-12/MEC-7 tubulins and a kinase candidate [500 nM recombinant MBK-2m or 475 nM CDK1/Cyclin B (Thermo Fisher; Cat# PV3292)] were incubated in MEM buffer [100 mM MES ...
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bioRxiv - Systems Biology 2023Quote: ... washed once with 20 µL buffer A and finally eluted with 20 µL buffer B to a 96-well plate (Thermo Fisher Scientific, AB1300), and vacuum dried (15 min at 60°C) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 M sorbitol) containing 100 or 500 µg/ml zeocin or YPD (YPDS without sorbitol) containing 200 µg/ml hygromycin B (Invitrogen, Thermo Fisher Scientific) already after 2 hours of incubation in 1 M sorbitol ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips were transferred to new tissue culture dishes containing 2 mL of maintenance medium (neurobasal supplemented with 1x B-27 Supplement, 1x Glutamax, and 50 U/mL penicillin-streptomycin (Thermo Fisher, Waltham, MA)) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% foetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (PSA: Penicillin, Streptomycin and Amphotericin B) (Thermo Fisher Scientific, Waltham MA, USA).
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bioRxiv - Biochemistry 2022Quote: ... The bound FL-TgGAC was eluted after incubation with TEV protease in buffer B and subsequently loaded onto a HisPur™ Ni-NTA Resin column (Thermo Scientific™). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... Human endothelial cells in muscles were stained with biotinylated Ulex Europaeus Agglutinin I Lectin (UEA I, B-1065-2, Vectorlab, 1:100 dilution) and FITC-streptavidin (11-4317-87, Invitrogen, 1:250 dilution). Nuclei was stained with DAPI ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... Neurovascular components were then pooled and re-suspended in 4 mL of buffer B (HBSS 1X Ca2+/ Mg2+ free with phenol red (Gibco, Waltham, MA, USA), 10 mM HEPES (Gibco ...
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bioRxiv - Synthetic Biology 2024Quote: MetBo2 (polyoma middle T oncogene–induced mouse mammary tumor on a syngeneic Friend Virus B NIH Jackson (FVB) background)[23] cells were maintained in 1X Dulbecco’s Modified Eagle Medium (DMEM) (Thermofisher Scientific; Cat. No. 11995065) with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... The following day cells were centrifuged at 2,000 x g for 10 minutes and the cell pellet was lysed using 8 mL of B-PER Bacterial Protein Extraction Reagent (Thermo Scientific, Waltham, MA) per gram of pellet with gentle shaking for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% antibiotic-antimycotic (AA: penicillin 100 U/mL, streptomycin sulfate 100 U/mL, 412 amphotericin B 0.25 mg/mL; Gibco, Cat. no. 15240-062), and 1% non-essential amino acids (NEAA ...
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bioRxiv - Neuroscience 2023Quote: ... A small hole was made in the eye using a sterile 34-gauge needle and ∼0.5 μl of cholera toxin subunit B conjugated with Alexa Fluor 488 (CTB-488, ThermoFisher Scientific, Catalogue Number: C34775) diluted in 0.9% sterile saline was intravitreally pressure-injected into the right eye using a pulled-glass micropipette coupled to a Picospritzer (Parker Hannifin).
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bioRxiv - Plant Biology 2024Quote: ... Following thawing on ice the cells were resuspended in bacterial protein extraction reagent B-PER (5 ml/g fresh weight) (Thermo Scientific, Waltham, USA) supplemented with Protease Inhibitor Mix-B (Serva ...
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bioRxiv - Bioengineering 2024Quote: ... 3D clusters were started to incubate in CODM supplemented with B-27 Supplement with vitamin A (Gibco, Thermo Fisher Scientific, Cat no. 17504044). At this step 3D clusters were differentiated into BOs in three-different culture conditions including static culture ...
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bioRxiv - Plant Biology 2020Quote: qRT-PCR analysis was performed using an Absolute Blue qPCR SYBR Green ROX Mix (AB-4162/B) kit (Thermo Fisher Scientific, Waltham, MA, USA). Reactions were performed using a Rotor-Gene 6000 cycler (Corbett Research ...
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bioRxiv - Systems Biology 2022Quote: For micro-flow UHPLC-MS/MS analysis 2 μL were injected into vanquish UHPLC system coupled to a Q-Exactive (setup A) or a Q-Exactive HF (setup B) quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) with an Agilent 1260 quaternary HPLC pump (Agilent ...
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bioRxiv - Physiology 2022Quote: Whole TA muscle was used for RNA isolation. 50 mg cryopreserved TA muscle was homogenised (Potter S 8533024, B. BRAUN) in 700 µl TRI reagent (Invitrogen, 11312940, Carlsbad, CA, USA) and incubated at room temperature (RT ...
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bioRxiv - Plant Biology 2020Quote: qRT-PCR analysis was performed using an Absolute Blue qPCR SYBR Green ROX Mix (AB-4162/B) kit (Thermo Fisher Scientific, Waltham, MA USA). Reactions were performed using a Rotor-Gene 6000 cycler (Corbett Research ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan) and antibiotic-antimycotic solution (Penicillin, Streptomycin, Amphotericin B. Gibco ThermoFisher, Carlsbad, CA, USA). Cultures were grown in 5% CO2 at 95% humidity ...
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bioRxiv - Bioengineering 2020Quote: ... the bacteria pellet was thawed on a water bath at 37 °C and lysed with B-PER™ complete bacteria protein extraction reagent (ThermoFisher Scientific, MA, USA). The released nanobody was purified via standard immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Neuroscience 2021Quote: Retrograde labeling from the CC and the cortical plate were performed by injecting subunit B of cholera toxin (CTB) conjugated to Alexa Fluor 555 (#C-34776, ThermoFisher Scientific. Massachusetts, the U.S.). Injections were performed in the CC ...
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bioRxiv - Neuroscience 2020Quote: ... and axial or intercostal muscles were injected with a solution of Cholera Toxin B Subunit-conjugated Alexa555 (Molecular Probes, Life Technologies, Burlington, ON, Canada), following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2021Quote: ... the plating medium was replaced by a culture medium (Neurobasal™ medium [21103-049, Thermo Fisher Scientific] containing B-27™ Supplement [17504-044, Thermo Fisher Scientific] ...
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bioRxiv - Biophysics 2022Quote: ... was spiked with 1% of Alexa 488-labeled HU-B mutant (containing an N-terminal Cys conjugated with Alexa Fluor™ 488 C5 Maleimide from Thermofisher; Cat no. A10254). The 488 nm laser was set at 100% for efficient bleaching and focused on a small circular region with a diameter of 1 μm ...
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bioRxiv - Plant Biology 2021Quote: ... and 95% ACN with 0.1% formic acid (solvent B)] at a flow rate of 300 nl/min and analyzed on LTQ Orbitrap Velos (Thermo Scientific, Waltham, MA, USA). 1.7kV was applied for ionization ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell expressing protein fragments with an MBP tag were lysed first using 1 mL Bacterial Protein Extraction Reagent (B-PER; Thermo Scientific Catalog number: 78243) and 1-2x of protease inhibitor (Thermo Scientific A32963) ...
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bioRxiv - Neuroscience 2022Quote: ... from E18 Sprague Dawley rats were thawed and cultured according to the manufacturer’s recommendations in a B-27 Plus Neuronal Culture System (Thermo Fisher Scientific, cat. no. A3653401). The thawing and culturing medium (NB Plus + ...
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bioRxiv - Microbiology 2019Quote: Human primary B cells from adenoids were separated from T cells by rosetting with sheep erythrocytes (purchased from Thermo Scientific Oxoid, cat. no. SR0051D) and purified by Ficoll density gradient centrifugation as described in the previous paragraph ...
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bioRxiv - Physiology 2020Quote: ... and homogenized with an automatic potter homogenizer (B. BRAUN, Germany) followed by RNA purification using the Ribo pure-kit (Thermo Fisher Scientific, Waltham, MA). RNA concentration and purity were measured using spectroscopy (Nanodrop Technologies ...
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bioRxiv - Genetics 2020Quote: ... Plates for hygromycin resistant transgenic animals were modified by adding working stock solution of 5 mg/mL Hygromycin B (Thermo Fisher Scientific, cat. 10687010) in water onto plates before use ...
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bioRxiv - Immunology 2021Quote: ... 2×108 spleen cells and inguinal lymph node cells (about 1×108 B cells) were mixed with 108 Sp2/0 cells and washed 3 times with RPMI1640 medium (Gibco, Thermo Fisher Scientific, Waltham, USA) without FCS ...
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bioRxiv - Cell Biology 2019Quote: ... for 120 min followed by 25-45 % buffer B for 15 min at a 400 nL/min flowrate on an Dionex Ultimate 3000 LC-system (Thermo Scientific, San Jose, CA). Eluted peptides were analysed with a Fusion Lumos mass spectrometry system (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... 100 IU/ml penicillin and 100 IU/ml streptomycin and 0.25 μg/ml Amphotericin B and 2 mM HEPES (Thermo Fisher Scientific, Waltham, MA, USA). Cells were cultured in the presence or absence of antibodies in 5% CO2 incubator at 37°C with humidity for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... samples were either run on a BD Symphony A3 or permeabilized and stained for 20 minutes at room temperature in medium B (Thermo Fisher Scientific, Waltham, MA) for intracellular markers ...
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bioRxiv - Immunology 2022Quote: ... Fluorescent antigen probes were used to detect VRC01gHL B cells with eOD-GT8 60-mer conjugated to Alexa Fluor 647 (Thermo Fisher Scientific, Catalog # A20173). Intracellular staining was performed with a fixation kit (BD Bioscience ...
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bioRxiv - Plant Biology 2022Quote: ... The seedlings were screened by microscopy for YFP and then fixed and processed for immunofluorescence microscopy using a 1:2000 dilution of monoclonal anti-α-tubulin B-5-1-2 antibody (Life Technologies; 32-2500) followed by 1:2000 dilution of Alexa-568 goat anti-mouse antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... All animals were injected with the retrograde tracer Cholera Toxin Subunit B in both the left (Alexa Fluor 647 Conjugate, C34778, Invitrogen, Carlsbad, CA, United States) and right (Alexa Fluor 488 Conjugate ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR analysis was performed using an Absolute Blue qPCR SYBR Green ROX Mix kit (AB-4162/B; Thermo Fisher Scientific, Waltham, MA, USA). Reactions were performed using a Rotor-Gene 6000 Cycler (Corbett Research ...
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bioRxiv - Biochemistry 2023Quote: ... Manufacturer B (Pierce™ Microcentrifuge Tubes no. 69715 and Low Protein Binding Microcentrifuge Tubes no. 90410, Thermo Fisher Scientific, Life Technologies GmbH, Carlsbad, USA) and manufacturer C (SafeSeal reaction tube no ...
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bioRxiv - Neuroscience 2023Quote: ... To obtain a single cell suspension the cells were mechanically dissociated with a glass Pasteur in neuronal culture medium (Neurobasal™ Plus Medium, Gibco A3582901, 1X B-27™ Plus Supplement, Gibco, A35828-01); the cell concentration was calculated with the T20 automated Cell Counter (Biorad) ...
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bioRxiv - Microbiology 2023Quote: ... b-galactosidase activity was measured using the Tropix Galacto-Light Plus™ chemiluminescent kit according to the manufacturer’s protocol (Applied Biosystems, Foster City, CA).