Labshake search
Citations for Thermo Fisher :
2651 - 2700 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2022Quote: ... The activation step consisted in the washing with 20 μl of solvent B (0.1 % FA in ACN, Fisher Scientific AG, Switzerland), followed by the conditioning with 20 μl of solvent A ...
-
bioRxiv - Immunology 2019Quote: ... The gating strategy was such that memory B cells (CD3− CD14− CD16− CD19+ IgD− IgM−) were sorted into B cell media (IMDM medium, GIBCO; 10% heat-inactivated low IgG FBS, Life Technologies; 5 ml GlutaMAX ...
-
bioRxiv - Neuroscience 2020Quote: The retrograde tracer cholera toxin subunit B (CTB) conjugated with either Alexa Fluor™ 647 or 555 (CTB-647 or CTB-555, respectively) was purchased from Invitrogen, Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Microbiology 2020Quote: Proteins were extracted from bioreactor cell pellets using glass bead beating (acid, washed, 0.1 mm diameter) in a suspension containing B-PER reagent (Thermo Scientific, Germany) and TEAB buffer (50 mM TEAB ...
-
bioRxiv - Microbiology 2020Quote: ... water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid) were driven and controlled by a Dionex Ultimate 3000 RPLC nano system (Thermo Fisher Scientific). An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18 ...
-
bioRxiv - Immunology 2019Quote: ... CRL-1596) Burkitt’s lymphoma B cell line was maintained in complete RPMI 1640 media supplemented with 10% FBS (cR10; Invitrogen, Carlsbad, CA). Primary human B cells ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
-
bioRxiv - Cell Biology 2021Quote: ... the U937 cells were selected with appropriate antibiotics (Zeocin 200µg/mL, G418 500 µg/mL, or Hygromycin B 200µg/mL; Thermo Fisher, Carl Roth, Thermo Fisher, respectively). A list of used primers can be found in the Supplementary information.
-
bioRxiv - Biochemistry 2021Quote: ... then subcloned into a T7 expression vector with an ampicillin selective marker (pRSET-B) (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA) by Gibson assembly ...
-
bioRxiv - Neuroscience 2021Quote: ... we injected fluorescently-labeled cholera toxin subunit B (CTB, 500 µg / 100 µL, Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA) into ipsilateral mPFC (Nanofil Syringe and Pump UMP3 ...
-
bioRxiv - Pathology 2021Quote: ... Stained sections were treated with Sudan Black (0.03% Sudan Black B in 70% ethanol) to quench endogenous fluorescence and coverslipped with DAPI containing Prolong Diamond Mountant (Fisher Scientific). Antibody labelled sections were imaged via a wide-field fluorescence microscope system (Nikon Eclipse 90i microscope ...
-
bioRxiv - Developmental Biology 2021Quote: ... Peptides were eluted along a 90 min gradient from 6 to 40% Buffer B (see StageTip purification) with the EASY□nLC 1,200 system (Thermo Fisher Scientific). Measurement was done on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2020Quote: ... the gypsy backbone used in previous publication (11) was amplified and cloned into the NotI/XbaI sites of pAc5.1/V5-His version B (V411020, Thermo Fisher Scientific). To synthesize the final pAc-gypsy-H2B-mCherry vector ...
-
bioRxiv - Immunology 2020Quote: ... the media was removed and replaced with cRF10 containing 1 mg/mL of hygromycin B solution from Streptomyces hydroscopicus (Invitrogen, USA). Media was refreshed as needed until drug selection was complete and all cells were fluorescent ...
-
bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... Preformed conjugates for antigen-specific B cell sorting were made as described (46) using streptavidin conjugated to PE or AlexaFluor 647 (Life Technologies). B cell probes were made using clade B JR-CSF gp120 ...
-
bioRxiv - Microbiology 2021Quote: ... water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid) were driven and controlled by a Dionex Ultimate 3000 RPLC nano system (Thermo Fisher Scientific). Gradient elution was performed at 300 nl min−1 ...
-
bioRxiv - Immunology 2021Quote: ... Ct values for Asns and the housekeeping gene Ppib (Cyclophilin B) were determined by quantitative real-time PCR on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems), using the Platinum® SYBR® Green qPCR SuperMix-UDG with ROX reference dye (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... a 1 µl Hamilton syringe (25s-gauge needle) filled with 150nL of 1% CT-B (1 mg/mL; Invitrogen, OR, USA) was used to pressure inject the retrograde tracer (150nL ...
-
bioRxiv - Immunology 2022Quote: ... and 1% antibiotic-antimycotic (100 μg/mL Streptomycin, 0.25 ug/mL Amphotericin B, & 100 U/mL Penicillin; Gibco, ThermoFisher, Waltham, MA) as previously described (12 ...
-
bioRxiv - Developmental Biology 2022Quote: ... HNPR1 and b-galactosidase mRNAs from the corresponding pCS2+ expression vector was performed using the Message Machine Kit (Ambion, Austin, TX).
-
bioRxiv - Microbiology 2022Quote: ... Samples were resuspended in a volume of solution B (an equal volume of solution A and LDS sample buffer, Life Technologies) ranging from 70 to 200 μl in proportion to their OD600nm ...
-
bioRxiv - Cell Biology 2022Quote: ... Bone marrow-derived B cells were cultured on gelatinized plates containing S17 feeder cells in RPMI culture medium (GIBCO, Cat#12633012) containing 20%-FBS (GIBCO ...
-
bioRxiv - Immunology 2022Quote: ... Cells were counted again and resuspended in 3 × 106 cells/mL before adding CC4F-AM (LiveBLAzer FRET-B/G Loading Kit with CCF4-AM, ThermoFisher Scientific). Cells were incubated for 1 hour ...
-
bioRxiv - Immunology 2022Quote: ... and 1% antibiotic-antimycotic (100 μg/mL Streptomycin, 0.25 ug/mL Amphotericin B, & 100 U/mL Penicillin; Gibco, ThermoFisher, Waltham, MA) as previously described (12 ...
-
bioRxiv - Immunology 2022Quote: ... cells were washed again in complete B cell media and 1×106 cells were resuspended in 50 µl of PBS containing LIVE/DEAD (Thermo Scientific) and human FC block (1:50 ...
-
bioRxiv - Plant Biology 2022Quote: ... from cauline leaves of flowering plants by measuring the quantity of 4-methylumbelliferone product generated from the substrate 4-methylumbelliferyl-b-D-glucuronide (Duchefa) on a fluorometer (Fluoroscan II; Thermo Scientific).
-
bioRxiv - Neuroscience 2022Quote: ... Throughout imaging hESCs were maintained in mTeSR plus containing 10µM Y-27632 and Penicillin-Streptomycin-Amphotericin B (Anti-Anti, Thermo Fisher Scientific). Additionally ...
-
bioRxiv - Neuroscience 2023Quote: ... at RT for 10 min and a final wash with 0.01x Buffer B for 1 min the coverslips were mounted in Fluoromount G (Thermo Fisher Scientific) and imaged at the Imaging Facility of the Max Planck Institute for Biological Intelligence ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by three more washes of Buffer B and one wash with 10 % formamide in 2x saline-sodium citrate (SSC) buffer (ThermoFisher AM9770). Cells were resuspended in the formamide/SSC solution and split evenly into two replicate samples for hybridization ...
-
bioRxiv - Biochemistry 2022Quote: ... 5% ACN) and buffer B (100 % ACN) and interfaced online with the Orbitrap Exploris 480 MS (Thermo Fisher Scientific, Bremen, Germany) using Xcalibur (tune version 3.0) ...
-
bioRxiv - Neuroscience 2023Quote: ... the retrograde tracer Cholera Toxin Subunit B conjugated with Alexa Fluor 555 or 674 (CTB 555 or CTB 647, 1 mg/ml, Invitrogen, USA) was bilaterally injected with a micropipette connected to Nanoject III microinjector (Drummond Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: Resting primary B and T cells were isolated from mouse spleens by negative selection using Mouse CD43 (Untouched™ B Cells) and Untouched Mouse T Cells Dynabeads respectively (Invitrogen). For experiments done in quiescent splenocytes ...
-
bioRxiv - Cancer Biology 2022Quote: ... and from 32% to 95% Buffer B from 65 to 68 minutes at a flow rate of approximately 110nl/min delivered on an Accela pump (Thermo Fisher), where Buffer A composition is 97.5% water ...
-
bioRxiv - Immunology 2022Quote: The stable J558L mouse B cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco, Catalog number 11875135) with 10% FBS ...
-
bioRxiv - Neuroscience 2022Quote: ... media was change to Neurobasal Plus medium (NB-Plus) composed of B-27™ Plus Neuronal Culture System (Thermo Fisher Scientific) supplemented with Glutamax 0.25% (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... and medium from day 3-7 is Neurobasal Plus medium (NB-Plus) supplemented with B-27 μg/mL (Thermo Fisher Scientific), GlutaMax 10 μg/mL (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants from the second centrifugation step were then dialysed overnight at 4 °C against ice-cold buffer B (50 mM Tris pH 7.5, 150 mM NaCl) in SnakeSkin dialysis tubing (Molecular weight cutoff 3 kDa, Thermo Fisher Scientific) until the sample reached pH 7.5 ...
-
bioRxiv - Neuroscience 2024Quote: ... NPCs were cultured in 2% B-27-supplemented Neurobasal CTS Media (plus 2 uM L-glutamine and 0.2% vol/vol penicillin–streptomycin; Thermo Fisher Scientific) plus 20 ng/ml BDNF ...
-
bioRxiv - Pathology 2024Quote: ... Protein samples were denatured at 70° C for 10 min after combining with 5% b-mercaptoethanol and 1X NuPAGE™ LDS Sample Buffer (Cat. No. NP0007, ThermoFisher) and run in 4-12% Bis-Tris gels (Cat ...
-
bioRxiv - Neuroscience 2023Quote: ... we then injected 0.3 μL of the retrograde tracer cholera toxin subunit B (CTB) conjugated with AlexaFluor-594 and AlexaFluor-488 (Thermo Fisher Scientific) into the msNAc and vsNAc ...
-
bioRxiv - Systems Biology 2024Quote: ... a mixture of 50:50 carboxylate-modified Sera-Mag SpeedBeads type A and B (Cytiva) were washed three times with ddH2O (Barstead GenPure, Thermo Scientific) on a magnetic rack and 1 mg of combined beads were added to the E.coli sample ...
-
bioRxiv - Systems Biology 2024Quote: ... using a 98 min gradient with a 250 nl/min flow rate of increasing Buffer B (90% ACN, 0.1% FA) concentration (from 2% to 60%) on a High Performance Liquid Chromatography (HPLC) system (Thermo Fisher Scientific) and analyzed on a Q Exactive Plus instrument (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2024Quote: ... was collected in an Eppendorf tube and solubilized in a suspension buffer consisting of 175 µL B-PER reagent (Thermo Scientific) and 175 µL TEAB buffer (50 mM TEAB ...
-
bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% v/v fetal bovine serum (FBS) (Vistech, SE100-B) and 1% v/v penicillin/streptomycin (Gibco, Cat# 15140122) at 37°C in a 5% CO2 incubator ...
-
bioRxiv - Biochemistry 2024Quote: ... and then in a GC B cell cocktail including: rat anti-mouse CD19 clone 1D3 PerCP-Cy5.5 conjugate (Fisher Scientific, BDB551001), hamster anti-mouse CD95 clone JO2 PE conjugate (Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: B cells were isolated from splenic single-cell suspension using MagniSort Streptavidin Negative Selection Beads (Thermo Fisher Scientific, MSNB-6002-74) and biotinylated antibodies against Ter119 (BioLegend ...
-
bioRxiv - Cell Biology 2024Quote: ... human BAK was cloned into a version of the retroviral Peak12-MMP vector containing an IRES-hygromycin cassette for selection in 200 μg/ml hygromycin B (Invitrogen, 10687010). The cDNAs for all BH3-only members ...
-
bioRxiv - Cancer Biology 2023Quote: ... Fluorescently labeled secondary antibodies (Alexa Fluor 488) were used to stain tissues for 1 hr at room temperature followed by applying Sudan Black B (Acros Organics) to reduce autofluorescence ...
-
bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) at 2 µg/mL were coated onto 96 well ELISA plates (Immulon 2 HB, Thermo Fisher) at 4°C overnight ...