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Citations for Thermo Fisher :
2551 - 2600 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Drd1afl/fl mice received bilateral injections of Alexa Fluor 594-conjugated Cholera Toxin Subunit B in PBS (CTB-594, 1.0 mg mL−1, no. C-34776, Thermo Fisher) in either LH or VTA brain regions.
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bioRxiv - Cell Biology 2023Quote: ... using buffer A (0.1% formic acid, FA) and buffer B (95% ACN, 0.1% FA) and an Orbitrap Eclipse Tribrid MS (Thermo Fisher Scientific). All fractions were redissolved in buffer A (0.1% FA ...
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bioRxiv - Genomics 2023Quote: ... EBs were plated on polyornithine/laminin-coated plates and cultured in N2/B-27 media containing 1 μg/mL laminin (Invitrogen). Neural rosettes were harvested by treating 14-day-old EBs with STEMdiff Neural Rosette Selection Reagent (Stem Cell Technologies ...
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bioRxiv - Genetics 2023Quote: ... The proliferation of primary B cells undergoing CSR was analyzed using the Cell Trace Violet (CTV) kit (ThermoFisher Scientific, C34557) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in growth media containing octadecyl rhodamine B chloride (R18, ThermoFisher, 1:1000 dilution of 10mg/ml stock), centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng/mL rh FGF-b, 25 µg/mL rh insulin, 4% FBS (PCS-950-040, ATCC) and penicillin-streptomycin (Gibco). 24-96 hrs after passaging ...
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bioRxiv - Microbiology 2023Quote: ... consisting of 10% fetal bovine serum and 0.1% Gibco™ Antibiotic-Antimycotic (10,000 units/mL penicillin, 10,000ug/mL streptomycin, 25ug/mL amphotericin B) (Thermo Scientific, USA). Cells were cultured for 7 days at 37°C in a carbogen (95% O2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Peptides were eluted along an optimized 90 min gradient from 6 to 40% Mixture B (80% ACN/0.5% formic acid) with an EASY-nLC 1,200 system (Thermo Fisher Scientific). Spray voltage was set to 2.2 kV ...
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bioRxiv - Immunology 2023Quote: Raji B cells were loaded with 500 nM calcein-AM (Life Technology, Waltham, Massachusetts, USA) in AIM V medium (ThermoFisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... was incubated with 5 µl Zenon component A solution for five minutes and then with 5 µl Zenon component B solution (ZenonTM Mouse IgG1 Labeling Kit Z-25006, Alexa Fluor 568, Invitrogen). PBS with 0.1% triton was added to the Zenon-labeled antibody mix to a final 1:75 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... HyClone) and 1× Antibiotic/Antimycotic (100 units/ml penicillin, 100 µg/ml streptomycin and 250 ng/ml amphotericin B; Gibco) except specified otherwise.
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bioRxiv - Cancer Biology 2023Quote: ... The measurement of lysosomal pH and Cathepsin B activity was evaluated with LysoTrackerTM Red DND-99 probe (InvitrogenTM, ThermoFisher Scientific) and the Magic Red® kit (Immunochemistry Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were plated on 0.4% gelatin-coated 60 mm culture dishes (Gelatin Type B Powder, Sigma-Aldrich, G9391; ThermoFisher Scientific Cell Culture Petri Dishes ...
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bioRxiv - Genomics 2023Quote: ... 0.10% (v/v) formic acid in water and (B) 0.10% (v/v) formic acid in acetonitrile (LC-MS optima, Fisher Scientific). Gradient conditions were as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v) methyl cellulose and B-27 (1/50, Life technologies).
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bioRxiv - Cell Biology 2023Quote: ... HCT116 cells were transfected with 2μg of total DNA of px330 cas9 and homology arms plasmids (mAID-mClover-HygR and mAID-mClover-NeoR for AID cell line and APEX2-mClover-HygR and APEX2-mClover-NeoR for APEX cell line) in 1:1:1 ratio and selected with 100 μg/mL hygromycin B (Invitrogen) and 700μg/ml of Geneticin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... with 1x PBS. After washing, 1 ml of differentiation medium (10% FBS (Millipore, Cat.No. ES-009-B, 1X L-Glutamine (Thermo Fisher, Cat.No.25030081 ...
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bioRxiv - Plant Biology 2023Quote: ... YPD medium at 28°C for 48 hours with 200 rpm shaking according to manufacturer’s instructions (pGAPZαA, B, & C Pichia pastoris Expression Vectors, Invitrogen; Carlsbad, USA). Protein purification was performed by using Ni-NTA-matrix according to manufacturer’s instructions (Ni-Sepharose™ 6 Fast-Flow ...
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bioRxiv - Neuroscience 2023Quote: ... (6) Negative photoresist SU-8 2000.5 was mixed with Lissamine rhodamine B ethylenediamine (RhBen; ∼10 μg/ml; Thermo Fisher Scientific) and placed in the dark at room temperature (RT ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Cells were passaged into a 10 cm dish and subsequently selected for resistance to 200 μg/mL hygromycin B (Gibco). For knockdown experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... were inoculated in 1X M199 liquid medium supplemented with 10 % (v/v) FBS and 30 μg/mL hygromycin B (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... Thermo Fisher Scientific) and RAN (housekeeping in Jurkats, B cells, monocytes, NK92-MI, SA-15837, Invitrogen™, Thermo Fisher Scientific). The linear range of this assay was identified for each probeset in each cell type ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were expanded to a T-25 flask in growth medium with 0.5 mg/mL Hygromycin B (Thermo Fisher). The selection process continued by replacing the growth media with Hygromycin B ...
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bioRxiv - Bioengineering 2023Quote: ... 10 µL of the grown pre-culture was re-inoculated to MTP-R48-B FlowerPlate (m2p-labs) containing 1 mL minimal medium + Pen/Strep (Invitrogen). The plate was incubated 48 h in Biolector® at 30°C ...
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Glioblastoma cells use an integrin- and CD44-mediated motor-clutch mode of migration in brain tissuebioRxiv - Cancer Biology 2023Quote: ... were cultured on T25 plastic culture dishes coated in 10% Matrigel with DMEM/F12 with 1x B-27 supplement (12587010; Gibco) and 1x P/S.
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal neurons were prepared from rat E18 embryos and maintained in Neurobasal Medium supplemented with B-27 and 2 mM L-glutamine (Invitrogen) for 18-21 days in vitro (DIV).
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bioRxiv - Neuroscience 2023Quote: ... CMV-tara was generated by digesting UAS-tara with BglII and XbaI and inserting the tara-B coding sequence into the BamHI and XbaI sites in the pCDNA3 vector (Invitrogen). UAS-Pdp1::V5 was generated by digesting UAS-Pdp1 (Zheng et al. ...
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bioRxiv - Cell Biology 2023Quote: ... we selected for α-arrestin-GFP stable cell lines by maintaining cells in Schneider’s Drosophila Medium supplemented with 200 μM Hygromycin B (#40-005, Fisher Scientific). The stable cells were transferred into T25 cm2 flasks to repopulate ...
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bioRxiv - Immunology 2024Quote: ... a cell line model for TNBC) was stably transfected with PiggyBac ROPN1/B-GFP DNA using Lipofectamine P3000 transfection reagent kit (Invitrogen) and Transposase Expression vector DNA (System Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... lyophilized and resuspended in Procartaplex Universal Assay Buffer and stored at −80°C prior to measurement of TGF-B using Procartaplex Single-plex assay (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... Thermo Fisher Scientific) and RAN (housekeeping in Jurkats, B cells, monocytes, NK92-MI, SA-15837, Invitrogen™, Thermo Fisher Scientific). The linear range of this assay was identified for each probeset in each cell type ...
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bioRxiv - Microbiology 2024Quote: ... 293T cells were transfected with 1ug of a plasmid expressing the B/Florida/04/2006 NA under a CMV promoter using LipofectamineTM 3000 transfection reagent kit (Invitrogen). Cells were harvested and used for absorption after 48 hours of transfection.
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bioRxiv - Developmental Biology 2024Quote: ... and DsRed were cloned into pcDNA6 vector between the XhoI and XbaI sites (pcDNA6/Myc-His B; Invitrogen, Waltham, MA) with a poly(A)-tail of 84 nucleotides (Wakai et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... LC mobile phase solvents and sample dilutions used 0.1% formic acid in water (Buffer A) and 0.1% formic acid in 80% acetonitrile (Buffer B) (Optima™ LC/MS, ThermoFisher). Data acquisition was performed using the instrument supplied Xcalibur™ (version 4.1 ...
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bioRxiv - Biophysics 2024Quote: ... The dissociated cells were resuspended in culture medium (Neurobasal A containing 2% B-27 plus (Thermo Fisher Scientific Inc., MA)) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... in deionised water (eluent A) to 0.1% formic acid in acetonitrile (LC-MS grade, Fisher Scientific, Loughborough, UK; eluent B) at a flow rate of 0.5 ml min-1 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v) methylcellulose and B-27 (1/50, Life technologies). Retinal explants were treated with 240 ng·ml-1 SHH C24II (R&D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... DRGs cultures were grown at 37 °C in Neurobasal-A medium supplemented with 2% B-27 and 1% Glutamax-I (Gibco).
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bioRxiv - Immunology 2024Quote: RNA was extracted from isolated B cells and ASCs using the PureLink RNA Mini Kit (Thermo Fisher Scientific, Cat# 12183025). RNA was quantified using a NanoDrop Onec (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... GFP (Appendix B (Scholz et al., 2000)) was biotinylated using EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, cat. no. A39259) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were rinsed thrice with PBS and incubated with phalloidin–tetramethylrhodamine B isothiocyanate (Phalloidin-TRITC, 1:100, Invitrogen, D3571) for 30 min in the dark ...
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bioRxiv - Microbiology 2024Quote: ... 293TT cells were used for generating HPV16 pseudoviruss were cultured in high-glucose DMEM media supplied with 10% bovine growth serum (BGS, HyClone SH30541.03) and 165ug/ml hygromycin B (Invitrogen 10687010) for selection ...
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bioRxiv - Cancer Biology 2021Quote: ... using a binary solvent system of 2% acetonitrile with 0.1% formic acid (Solvent A) and 80% acetonitrile with 0.1% formic acid (Solvent B) in an Easy nLC-1000 system (Thermo Fisher Scientific). 2 μL of peptide solution was loaded using Solvent A onto an Acclaim PepMap100 C18 trap column (2 cm x 75 μm inner diameter) ...
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bioRxiv - Cancer Biology 2021Quote: ... water with 0.2% formic acid and B: acetonitrile with 0.2% formic acid) were driven and controlled by a Dionex Ultimate 3000 RPLC nano system (Thermo Fisher Scientific). An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18 ...
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bioRxiv - Cell Biology 2020Quote: U2OS cells were engineered to stably express histone H2A type 1 whose N-terminus is labeled with HaloTag (Halo-H2A) by retroviral transfection and selection with 200 μg ml−1 hygromycin B (#10687010, Thermo Fisher). NES-mCherry-LINuS (Niopek et al. ...
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bioRxiv - Cell Biology 2020Quote: ... A gradient consisting of 0% to 40% B in A (A: 0.1% formic acid [Fisher Scientific, A117], 6% acetonitrile [Fisher Scientific, A955] ...
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bioRxiv - Genetics 2021Quote: ... Log R Ratio (LRR) and B Allele Frequency (BAF) were extracted from the Axiom™ Analysis Suite Software 4.0.3.3 (Thermo Fisher Scientific, USA) using the Axiom® CNV Summary Tools 1.1 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and cultured in suspension in KBM neural stem cell medium (Kohjin Bio, Saitama, Japan) supplemented with B-27 (Thermo Fisher Scientific), 20 ng/ml FGF-2 (PeproTech ...
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bioRxiv - Neuroscience 2021Quote: ... and fluorescent retrograde tracer cholera toxin B subunit conjugated to fluorophore Alexa 488 (CTB488, Thermo Fisher Scientific, Waltham, MA, USA, C34775) were used to label neurons that projected from the target regions ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed using double nucleic acid detection kit for respiratory syncytial virus (A/B) (Shenzhen shengkeyuan) with the ABI Q7 (Applied Biosystems). The reaction was conducted according to the manufacturer’s instructions with total volume of 20 μL.