Labshake search
Citations for Thermo Fisher :
2451 - 2500 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... 2% solvent B (solvent B: 80% acetonitrile containing 0.1% formic acid) and directly sprayed into a Q-Exactive Plus mass spectrometer (Thermo Scientific) for the data acquisition ...
-
bioRxiv - Cell Biology 2022Quote: HEK293T cells (from B. D. Dynlacht) were grown in DMEM (Nacalai tesque) supplemented with 10% Calf Serum (Thermo Fisher Scientific) and 100 units/ml penicillin and 100 μg/ml streptomycin (P/S ...
-
bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco] ...
-
bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco], 1/100 N-2 supplement [Gibco] ...
-
bioRxiv - Cell Biology 2021Quote: ... The lentivirus-transduced HEK293T and hTERT-RPE1 cells to express mouse CatSpertS:WT-EGFP and CatSperτS:Mut-EGFP were passaged one time and cultured in the FBS- and Pen/Strep-containing media supplemented with and 50 μg/ml (HEK293T cells) or 20 μg/ml (hTERT-RPE1) hygromycin B (Invitrogen). Stable protein expression was checked by epifluorescence microscope (ZOE Fluorescent Cell Imager ...
-
bioRxiv - Biophysics 2021Quote: ... and the cells were plated on 50 μg/ml of poly-D-lysine-coated cover glasses in Neurobasal medium (Biological Industries, Israel) supplemented with 2% B-27 (Invitrogen), GlutaMAX-I 2mM (Invitrogen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with 100 μl prewarmed PBS and then 100 μl prewarmed recording media supplemented with 1x B-27 (ThermoFisher) and 100 μg/ml Luciferin was added ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 1 % antibiotic/antimycotic solution (Thermo Fisher Scientific, containing 10,000 units/mL of penicillin, 10.000 μg/mL of streptomycin, and 25 μg/mL of Gibco Amphotericin B). After 14 days ...
-
bioRxiv - Neuroscience 2021Quote: ... CHO-Q3 stable cells were maintained under dual selection with Zeocin (100 μg/ml) and hygromycin B (600 μg/ml) in F-12 nutrient mixture medium (GIBCO/Invitrogen) supplemented with 10% fetal bovine serum (ATLANTA Biologicals) ...
-
bioRxiv - Immunology 2020Quote: Proliferation of OT-II and B cells was assessed using CFSE or CellTrace Violet (CTV) labelling as specified by the manufacturer (Thermofisher). A total of 2×105 purified naïve B cells were CTV-stained and co-cultured with purified CFSE-stained OT-II T cells at a 1:1 ratio together with antigen-coated beads or soluble antigen in a round-bottom 96-well plate ...
-
bioRxiv - Immunology 2020Quote: HLA class I-deficient 721.221 lymphoblastoid EBV-B cells were cultured in R10 medium (RPMI 1640 supplemented with 1% penicillin-streptomycin (Life Technologies) and 10% heat inactivated fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2022Quote: The coding sequence of the kinase domain of EiIF2K-A (1313 bp) and EiIF2K-B (1397 bp) was synthesized and ligated into the pYES-NT/C plasmid (ThermoFisher, kind gift of Dr ...
-
bioRxiv - Neuroscience 2022Quote: Projection neurons were retrograde labeled from their axon terminals by pressure injection of Alexa-conjugated Cholera Toxin B (Ctb, Invitrogen) or Fast Blue (Polysciences ...
-
bioRxiv - Neuroscience 2022Quote: ... SCPN (CBN + CSN) or CSN only were retrogradely labeled by bilateral injection of Cholera Toxin B subunit (CTB; Thermo Scientific) into either cerebral peduncles (at 3 injection sites ...
-
bioRxiv - Genetics 2022Quote: ... Hygromycin-resistant colonies emerging on the surface of the overlay after 3–5 days were excised and transferred to MEA amended with 100 µg/mL of hygromycin B (Invitrogen) (MEA+Hyg100) ...
-
bioRxiv - Immunology 2022Quote: ... CD19+ B cells were then stained using LIVE/DEAD aqua fixable dead cell stain kit (Molecular Probes, Thermo Fisher Scientific) to exclude dead cells ...
-
bioRxiv - Immunology 2022Quote: ... CD19+ B cells were then stained using LIVE/DEAD aqua fixable dead cell stain kit (Molecular Probes, Thermo Fisher Scientific) to exclude dead cells ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were thawed in ice with 1 ml of 1X lysis buffer B and Halt protease inhibitor cocktail (Thermo scientific)] and incubated in a rotator for 10 m ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Samples were centrifuged for 10 min at 4,500g and cell pellets were resuspended and lysed with B-PER reagent (Thermo Scientific) (1/10 of the initial volume of cosmid production for in vitro assays and 1/50 of the initial volume of production for in vivo assays ...
-
bioRxiv - Cell Biology 2019Quote: ... and incubated 15 min at RT in Grace’s medium containing 40 μg/ml Dextran-Rhodamine B 10,000 MW (Molecular Probes D1824), and immediately imaged after incubation ...
-
bioRxiv - Immunology 2019Quote: ... Primary B cells from C57BL/6J mice were resuspended in complete medium and labeled with 2 µM CFSE (Invitrogen, #C1157) for 8 min prior to being treated with CK-666 or CK-689 for 1 hr ...
-
bioRxiv - Microbiology 2019Quote: ... Transduced cells were selected and cultured in medium additionally supplemented with 2 μg/ml puromycin (Melford laboratories) or 200 μg/ml of hygromycin B (Invitrogen).
-
bioRxiv - Genomics 2019Quote: ... Protein amount was quantified by BCA protein assay reagents (Pierce) and normalized for loading on a 4–12% B-T NuPage gels (Invitrogen). After transfer on nitrocellulose membrane ...
-
bioRxiv - Immunology 2019Quote: ... Colonic lymphocytes were used without prior enrichment by CD8 microbeads and were maintained in R10 supplemented with 25ng/mL amphotericin B (Gibco), 40μg/mL gentamicin (Gibco) ...
-
bioRxiv - Biochemistry 2019Quote: ... Pelleted cells were lysed at room temperature for 20 min using B-PER bacterial protein extraction reagent (90078, Thermo Scientific) supplemented with 100 μg/mL lysozyme ...
-
bioRxiv - Genetics 2021Quote: Total RNA was isolated from the eggs at 24 hpo from a wild-type strain (p50T) and three b-4 mutant strains (e36, e37, and No. 903) using TRIzol (Invitrogen). Reverse transcription was then performed using the oligo (dT ...
-
bioRxiv - Plant Biology 2020Quote: ... SunTag-VP64 or SunTag-TET1 transgenic plants were selected on 1/2 MS medium + 35 μg/mL Hygromycin B (Invitrogen). The number of leaves at the time of flowering was calculated by counting the total number of rosette and cauline leaves.
-
bioRxiv - Synthetic Biology 2021Quote: ... coli were collected in an Eppendorf tube and solubilised in a suspension solution consisting of 200μL B-PER reagent (Thermo Scientific) and 200μL TEAB buffer (50 mM TEAB ...
-
bioRxiv - Microbiology 2020Quote: ... which was supplemented with Antibiotic-Antimycotic (100 U/ml penicillin-100 μg/ml streptomycin-0.25 ug/ml Amphotericin B) (Gibco; 15240062) and 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2020Quote: ... Cryopreserved PBMC from the SARS-CoV-2-infected participant and a SARS-naïve donor were thawed at 37°C and stained for SARS-CoV-2-specific memory B cells with a flow cytometry panel consisting of: a viability dye (7AAD, Invitrogen), CD14 PE-Cy5 ...
-
bioRxiv - Microbiology 2019Quote: ... The peptides were separated by increasing concentrations of buffer B (80% acetonitrile / 0.1% formic acid) for 158 min and analyzed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) operated in data-dependent acquisition mode using in-house ...
-
bioRxiv - Molecular Biology 2019Quote: ... exchanging half of the media with Serum-Free medium (DMEM w/o Phenol Red-GIBCO-, 25mM HEPES, L-Glu, Pen/Strep, 1% glucose, B-27 and N2 supplement-GIBCO-) every 2 days ...
-
bioRxiv - Immunology 2021Quote: ... 2×108 spleen cells and inguinal lymph node cells (about 1×108 B cells) were mixed with 108 Sp2/0 cells and washed 3 times with RPMI1640 medium (Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... The Ba/F3 pro-B cell line was obtained from Leibniz-Institute DSMZ and grown in RPMI-1640 (Life Technologies) with 10% FBS (Life technologies) ...
-
bioRxiv - Immunology 2021Quote: To determine the presence of GPR55 in B cells we performed fluorescent in situ hybridization (FISH) by using a murine Gpr55-probe (VB6-3216575, Affymetrix). The splenic sections were cut and collected in RNAse free slides (slides cleaned with RNAse ZAP) ...
-
bioRxiv - Bioengineering 2021Quote: ... Culture medium from day 0 until day 7 was RPMI1640 (HyClone, SH30027.01) plus B-27 serum-free supplement without insulin (Gibco, A1895601). From day 7 and onwards RPMI1640 with B-27 serum free supplement with insulin (Gibco ...
-
bioRxiv - Biochemistry 2021Quote: ... IL3-dependent murine pro-B cell (Ba/F3 cells, ATCC) were maintained in Roswell Park Memorial Institute (RPMI) media (Thermofisher) supplemented with 10% FBS and IL-3 and 1% penicillin/streptomycin ...
-
bioRxiv - Cancer Biology 2020Quote: ... quantitative-PCR was carried out using TaqMan™ Array Rodent MicroRNA A+B Cards Set v3.0 (Applied Biosystems™, 4444909). The data were analyzed using ViiA 7 Software and R program ...
-
bioRxiv - Biophysics 2021Quote: ... and the cells were plated on 50 μg/ml poly-D-lysine-coated cover glasses in Neurobasal medium (Biological Industries, Israel) supplemented with 2% B-27 (Invitrogen), GlutaMAX-I 2mM (Invitrogen) ...
-
bioRxiv - Cell Biology 2020Quote: ... Analysis of a 150 min gradient of 2% to 25% buffer B (0.1% formic acid in acetonitrile) was performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). The ion source was operated at 2.4kV and the ion transfer tube was set to 300°C ...
-
bioRxiv - Microbiology 2020Quote: ... Salivary glands from infected mosquitoes were hand dissected and collected in complete William’s B medium (William's E medium with Glutamax [Thermo Fisher, 32551-087], supplemented with 1X insulin/transferrin/selenium [Thermo Fisher, 41400-045] ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR amplicon was digested with EcoR I and Age I and cloned to the same sites in pAc5.1/V5-His B (Thermo Scientific) to express the His-tagged protein ...
-
bioRxiv - Immunology 2020Quote: Splenic mouse B lymphocytes were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, ThermoFisher Scientific, Paisley, United Kingdom) containing GlutaMax (200mM L-alanyl-L-glutamine-dipeptide) ...
-
bioRxiv - Immunology 2021Quote: ... Qβ-VLPs were then re-packaged with B-type 1668 CpGs (5″-TCC ATG ACG TTC CTG ATG CT-3″) with phosphorothioate backbone purchased from (InvitroGen). The re-packaging was confirmed by 1% agarose gel stained with SYBR Safe dye for 30min at 90V ...
-
bioRxiv - Immunology 2020Quote: ... -B and -C were sequenced in both directions using an ABI 3730XL DNA sequencer (Applied Biosystems, Foster City, CA, USA). HLA genotypes were assigned using the Assign 4.7 software (Conexio Genomics ...
-
bioRxiv - Immunology 2020Quote: The proliferation of B cells in skin and head kidney were assessed in control and survivor fish by injection wih EdU (Invitrogen) as previously reported (7) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and cultured at 5% O2 in Dulbecco’s modified essential medium (DMEM)/F12 with B-27 minus vitamin A (Life Technologies), 1% penicillin-streptomycin ...
-
bioRxiv - Molecular Biology 2020Quote: ... peptides were trapped at 3 μl/min on a 10 mm × 75 μm ID Acclaim Pepmap trap at 2% buffer B (buffer A: 0.1% formic acid (Fisher Scientific), buffer B ...
-
bioRxiv - Neuroscience 2021Quote: ... nwS1 was stereotaxically injected with 80-100 nl cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 (CTB-488, Invitrogen), and wS1 with CTB-Alexa Fluor 555 (CTB- 555 ...
-
bioRxiv - Cancer Biology 2021Quote: ... MEFs stably expressing inducible Poly94Q-CFP chains were transduced with lentivirus and selected with hygromycin B (Thermo Fisher Scientific, 10687010). Polyglutamine aggregates were induced by addition of Doxycyclin (5 μg/mL ...