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Citations for Thermo Fisher :
2851 - 2900 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... [10] and NME5mut (MHHi019-B) [10]) were generated from CD34pos cells using the CytoTune®-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: A glass micropipette filled with a solution containing AAV or cholera toxin b-subunit (CTb) conjugated with Alexa Fluor 488 (Alexa488) or Alexa594 (1 mg/ml; C22841 and C22842, Thermo Fisher Scientific) was perpendicularly inserted into the LPB ...
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bioRxiv - Systems Biology 2024Quote: ... cells were cultured for 72h with 10 nM SB431542 in culture media containing DMEM/F12 with 1x GlutaMAX/1x insulin-transferrin- selenium/1x NEAA/2% B-27 (Gibco 17504-044)/90 µM 2-Merceptoethanol ...
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bioRxiv - Genetics 2023Quote: ... the medium was removed and NIM was added with doxycycline plus 5 µg ml–1 puromycin and 250 µg ml–1 hygromycin B (Thermo Fisher Scientific) (NIM selection medium) ...
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bioRxiv - Immunology 2023Quote: To analyze proliferation in vitro, B cells (2 x 106, purified as above) were labeled with 5 µM CellTrace Violet (CTV) (Invitrogen, Waltham, MA) and then activated and cultured as above ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from day 2 LPS blasts seeded with sorted B cells using TRIzol reagent following the manufacturer’s instructions (Life Technologies, Carlsbad, CA). Genomic DNA was extracted from flow-purified naïve and GC B cells using lysis buffer (0.2% SDS ...
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bioRxiv - Microbiology 2023Quote: ... Cf2-Th/CD4+CCR5+ cells grown in the presence of 400 µg/ml G418 and 200 µg/ml hygromycin B selection antibiotics (both from Invitrogen, ThermoFisher Scientific). CEM ...
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bioRxiv - Systems Biology 2023Quote: ... then held at 90% B for 9 minutes at a flow rate of 300 nl/min delivered by an Easy1200 nLC system (Thermo Fisher Scientific). All MS1 spectra were collected with orbitrap detection at a 70,000 resolution and a scan range from 300 to 1,500 m/z ...
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bioRxiv - Microbiology 2023Quote: ... Cf2Th-CD4/CCR5 and Cf2Th-CD4/CXCR4 cells were grown in the same medium supplemented with 400 µg/ml G418 and 200 µg/ml hygromycin B (both from Invitrogen, ThermoFisher Scientific). Cf2Th-CCR5 cells were grown in the same medium supplemented with 400 µg/ml G418.
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0, ThermoFisher Scientific). For the data analysis ...
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bioRxiv - Cell Biology 2024Quote: ... 30 min 70% B at a flow rate of 200 nL/min using an UltiMate 3000 RSLCnano LC system (Thermo Fisher Scientific). The eluted peptides were detected using a quadrupole Orbitrap Exploris 480 hybrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... After selection for 2 weeks in DMEM supplemented with hygromycin B (250 μg/ml; Carl Roth) and blasticidin (4 μg/ml; Thermo Fisher Scientific), single-cell colonies were isolated ...
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bioRxiv - Immunology 2024Quote: ... using a 90 min gradient with a 250 nL/min flow rate of increasing Buffer B concentration (from 2% to 60%) on a High-Performance Liquid Chromatography (HPLC) system (Thermo Fisher Scientific) and ionized using an electrospray ionization (ESI ...
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bioRxiv - Molecular Biology 2024Quote: ... using a 90-minute gradient at a flow rate of 250 nL/min with increasing concentration of Buffer B (from 2 % to 60 %) on a high performance liquid chromatography (HPLC) system (Thermo Fisher Scientific), ionized with an electrospray ionization (ESI ...
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bioRxiv - Immunology 2021Quote: Bone marrow B factions were sorted and calcium influx was assayed with a Fluo-4 Direct™ Calcium Assay Kit (ThermoFisher cat# F10471). Briefly cells were incubated with Fluo-4 Direct™ at 37°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... the medium was replaced with RPMI medium containing CCF4-AM substrate from the LiveBLAzerTM FRET-B/G Loading Kit (ThermoFisher Scientific Waltham, MA). Cells were incubated in the dark at 37°C for 1h ...
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bioRxiv - Biochemistry 2020Quote: ... LC mobile phase solvents consisted of 0.1% formic acid in water (Buffer A) and 0.1% formic acid in acetonitrile (Buffer B, Optima™ LC/MS, Fisher Scientific, Pittsburgh, PA). Data acquisition was performed using the manufacturer-supplied otofControl (version 6.0 ...
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bioRxiv - Biochemistry 2020Quote: ... LC mobile phase solvents consisted of 0.1% formic acid in water (Buffer A) and 0.1% formic acid in acetonitrile (Buffer B, Optima™ LC/MS, Fisher Scientific, Pittsburgh, PA). Data acquisition was performed using the instrument supplied Xcalibur™ (version 4.1 ...
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bioRxiv - Bioengineering 2019Quote: ... Jurkat and Human B-lymphoid cell lines were initially tested for mycoplasma contamination and then cultured in RPMI-1640 medium (Life Technologies, Zug, Switzerland) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan) and antibiotic-antimycotic solution (Penicillin, Streptomycin, Amphotericin B. Gibco ThermoFisher, Carlsbad, CA, USA). Cultures were grown in 5% CO2 at 95% humidity ...
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bioRxiv - Plant Biology 2019Quote: ... The mobile phase consisted of A: 0.1% formic acid in water and B: 0.1% formic acid in 80% acetonitrile/20% water (Thermo Fisher Scientific, Waltham, MA, USA). At a flow rate of 500 nL/min ...
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bioRxiv - Microbiology 2019Quote: ... washed 3x with modified M63 and subjected to 3 cycles of freezing at −20°C and thawing before being lysed with B-PER Complete Bacterial Protein Extraction Reagent (from Thermo Scientific, Cat # 89821) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently isolated from medium via centrifugation (5000 rcf for 10 min) and lysed with B-PER (Thermo Fisher Scientific, Waltham, MA) for 15 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... β-lactamase CCF2-AM loading solutions from the Live-BLAzer FRET B/G Loading Kit was obtained from Life Technologies (Carlsbad, CA, USA); Filipin ...
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bioRxiv - Biochemistry 2020Quote: ... we lysed cell pellets by adding the following components to each gram of pellet: 4 mL of B-PER (Thermo Fisher Scientific, Inc.), 1 mg MgSO4 ...
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bioRxiv - Neuroscience 2021Quote: ... pairwise injections of 80 nL of 0.5% (wt/vol) fluorescently labeled cholera toxin B subunit (CTB-488, ThermoFisher C22841, CTB-647, ThermoFisher C34788). In C57BL6/J mice ...
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bioRxiv - Neuroscience 2020Quote: Mice used for retrograde tracing experiments received cholera toxin b subunit (CTB) conjugated with Alexa Fluor 488 and/or Alexa Fluor 647 (CTB-488 and CTB-647; ThermoFisher, C34775 and C34778).
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bioRxiv - Cell Biology 2021Quote: Strains from the tested collection were refreshed on YPD medium and then pin-spotted onto YPD with phloxine B (30 μM) (Acros Organics, Cat # 189470250) using 384-pin replicators with long pins (Singer ...
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bioRxiv - Systems Biology 2020Quote: ... and 4 μL of this dilution were used to transform One ShotTM ccd B Survival 2 T1R competent cells (Thermo Fisher Scientific, A10460). Transformants were selected with 33 μg/mL chloramphenicol (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... cat no: B-002000-UB-100) and 2 μL (A673) or 2.5 μL (CHLA10) Liopfectamine RNAiMAX transfection reagent (Life Technologies, cat no: 13778150). The mixture was incubated with intermittent rocking for 30 minutes prior to the addition of cells.
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bioRxiv - Biophysics 2020Quote: ... 10,000 units/mL of penicillin, 10,000 mg/mL of streptomycin and 25 mg/mL amphotericin B, Gibco, Thermo Fisher Scientific, 5% horse serum) for up to 7 days at 16 °C ...
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bioRxiv - Cell Biology 2020Quote: ... All cells from each tissue were resuspended with the D10 buffer to a concentration of 50-500 million cells per milliliter and stained with Calcein AM (Component A: AM) and Ethidium homodimer-1 (Component B: EH) in LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen, Cat. no. L3224) for 20 min on ice ...
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bioRxiv - Bioengineering 2022Quote: ... HEK293T cells were transfected with pCEP4-myc-hACE2 and cells were selected using 100 μg/mL of hygromycin B (Invitrogen, Logan, Utah, USA). The detailed procedure was delineated previously52 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated overnight with primary antibodies at 4°C in B/P buffer containing either 1:50 rabbit anti-fatty acid synthetase (MA5-14887; Invitrogen, Waltham, MA, USA) or 1:500 rabbit anti-peroxisome proliferator-activated receptor gamma (ab45036 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pellets were re-suspended and cultured in DMEM/F12 media supplemented with 10% dextran-coated charcoal stripped foetal bovine serum (DCC-FBS), antibiotic-antimycotic solution (10 U/ml penicillin, 10 µg/ml streptomycin and 0.25 µg/ml Amphotericin B (Thermo Fisher Scientific, Loughborough, U.K)) ...
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bioRxiv - Neuroscience 2020Quote: ... and cultured for 7 days in vitro in Neurobasal® medium supplemented with 2% v/v B-27® supplement medium and 1mM L-glutamine (all from Life Technologies) at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... The assay is based on the dilution of co-mixed N-(7-nitro-benz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine (N-NBD-PE) and N-(lissamine Rhodamine B sulfonyl)phosphatidylethanolamine (N-Rh-PE) (Molecular Probes, Eugene, OR, USA), whereby dilution due to membrane mixing results in increased N-NBD-PE fluorescence ...
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bioRxiv - Neuroscience 2021Quote: ... culture dishes were either custom-made 10-15mm diameter glass-bottom dishes [acid-washed coverslip (Fisher Scientific #12-541-B 22×22-1.5) affixed over a hole in a 35mm petri dish (Falcon #351008 ...
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bioRxiv - Neuroscience 2020Quote: ... the left hemisphere VMH was pressure injected with 180 nl of a bi-directional neural tracer cocktail consisting of 5 μg/μl retrograde Cholera toxin subunit B conjugated with Alexa Fluor 488 (Life Technologies, cat# C22841) and 50 μg/μl anterograde dextran amine 10,000 MW conjugated with Alexa Fluor 488 (Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... (containing five tandem copies of the NF-κB site from the IP10 gene) was introduced in the respective cells using Lipofectamine™ LTX Reagent and PLUS Reagents (Thermo Fisher Scientific). Luciferase activity was measured after 48 hours (with or without drug treatment ...
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bioRxiv - Neuroscience 2020Quote: ... coverslips were transferred to new tissue culture dishes containing 2 mL of maintenance medium (neurobasal supplemented with 1x B-27 Supplement, 1x Glutamax, and 50 U/mL penicillin-streptomycin (Thermo Fisher, Waltham, MA)) ...
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bioRxiv - Microbiology 2021Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) and B-cell (CD19-eFluor450, ThermoFisher Scientific, Grand Island, NY) markers after blocking with anti-mouse CD16/32 antibodies (BioLegend) ...
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bioRxiv - Synthetic Biology 2021Quote: ... resuspended in 5 mL of fresh TAP media and 200 uL of cells spread on 10 cm diameter plates containing 15 μg/mL Zeocin and 30 μg/mL Hygromycin B (Thermo Scientific, Waltham, MA) in TAP+15 g/L agar media.
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bioRxiv - Neuroscience 2020Quote: ... Neurovascular components were then pooled and re-suspended in 4 mL of buffer B (HBSS 1X Ca2+/ Mg2+ free with phenol red (Gibco, Waltham, MA, USA), 10 mM HEPES (Gibco ...
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bioRxiv - Genetics 2019Quote: ... Ten 150mm plates of confluent cells expressing each construct were grown overnight in media with a final concentration of 50 uM D-Biotin (Life technologies, B-20656).
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bioRxiv - Biochemistry 2019Quote: ... B-ALL cell lines were cultured in RPMI 1640 media supplemented with 10% heat-inactivated fetal bovine serum (ThermoFisher Scientific; cat. no. 10082147) and 2 mM L-Glutamine (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... pseudotuberculosis strains carrying complementation constructs (pGM930) and pMK-Bla (YopE-TEM, [87] using LiveBLAzer-FRET B/G Loading Kit (Life Technologies, Carlsbad, USA). Strains were pregrown in LB containing 0.1 % (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... Induced cells were lysed using B-PER Bacterial Protein Extraction Reagent (ThermoFischer Scientific cat. # 90084) and purified using HisPur Ni-NTA resin (ThermoFisher Scientific cat. # 88221) or a HisTrap HP column (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... Total RNA from single-sorted B cells was reverse transcribed in the original sorting plate with 150 ng Random Hexamer Primer (Thermo Scientific, Cat#S0142), 1 mM dNTP Mix (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... All solutions with the exception of Latrunculin B were utilized at room temperature and supplemented with a 1X protease inhibitor cocktail (Thermo Fisher Scientific, #78425) to minimize proteolysis ...