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Citations for Thermo Fisher :
2801 - 2850 of 2985 citations for Orexin B SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The suspension was then mixed with 100 ml of a wash buffer containing 1:10 B-PER in ice cold 1xPBS (diluted from 10X PBS stock, Fisher Scientific #BP3994) by pipetting the mixture up and down and gentle agitation ...
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bioRxiv - Immunology 2019Quote: ... Primary B cells from C57BL/6J mice were resuspended to 107 cell/ml in RPMI-1640 without phenol red (Life Technologies #32404014) plus 5 mM HEPES ...
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bioRxiv - Bioengineering 2019Quote: ... Tissues were cultured in iCell plating media (iCell CMM-100-010-001) with 1X penicillin/streptomycin/Amphotericin B (P/S/A, Gibco cat# 15240062). After 48h ...
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bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific] ...
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Microbiology 2021Quote: Cells expressing the eGFP-RBD3 probe [24] were grown in SD medium at 30° C until exponential phase(in the range 0.6-1 × 107 cells mL-1) and incubated with the mitochondrial marker rhodamine B hexyl ester perchlorate (Molecular Probes, Eugene, OR) 100 nM final concentration for about 5 min before imaging ...
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bioRxiv - Bioengineering 2020Quote: The IGFBP-derived NLS peptides (NLS-3 and NLS-5) were extracted from the bacteria using the B-Per 6xHis Fusion Protein Purification Kit (Thermo Scientific, USA), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Then the cells were incubated for 2 h at 37°C to allow cell adhesion with the volume completed using B-27™ Plus Neuronal Culture System (Gibco, A3653401), supplemented with penicillin (100 UI/mL)-streptomycin (100 μg/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... the media was changed and 48 hours post transfection cells were harvested either by resuspension in a) 2xSDS loading buffer (for western blotting) or b) TRIzol Reagent (Thermo Fisher Scientific) (for RNA purification ...
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bioRxiv - Genetics 2019Quote: ... The washed resin was resuspended in 900 ul of elution buffer (1XPBS, 5% SDS, 10 mM D-Biotin (Life technologies, B-20656), transferred to an eppendorf tube and boiled for 15 min ...
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bioRxiv - Neuroscience 2019Quote: A gene encoding Mus musculus TRAAK (UniProt O88454) was codon-optimized for yeast expression, synthesized (Genewiz, Inc.) and cloned into a modified pPICZ-B vector (Life Technologies Inc.) to create a human rhinovirus 3C protease-cleavable C-terminal EGFP-10x histidine fusion protein construct ...
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bioRxiv - Neuroscience 2019Quote: ... Dissociated cells were filtered through 40 µM strainer and washed twice in Hibernate-A media with B-27 (2%, Thermo Fisher Scientific), GlutaMAX (0.5 mM) ...
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bioRxiv - Neuroscience 2021Quote: Rats (N = 4) were infused unilaterally with AlexaFluor-594 conjugated cholera toxin B (CTb; 5 μg/μL; Life Technologies, San Diego, CA) into the mOFC (AP +4.1 ...
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bioRxiv - Neuroscience 2021Quote: ... 90 nl of Cre-expressing lentivirus (LVretro-Cre) (Knowland et al., 2017) was mixed with cholera-toxin B subunit (CTb) conjugated to Alexa 488 (Thermo Fisher Scientific) at 1:1 ratio and was injected into the GPe (in mm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... QBIHEK293 cells that expressed GC-A and GC-B were split the day before the experiment and harvested using an ethylenediaminetetraacetic acid (EDTA) solution (Versene, Invitrogen, ThermoFischer Scientific). Cells (6000/well GC-A-expressing ...
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bioRxiv - Cancer Biology 2020Quote: ... extracted and ethanol precipitated DNA (20 μg) was run in 1% agarose and Southern transferred (standard procedures) onto Biodyne B nylon membranes (Thermo Scientific, MO). Blots were probed with a 753 bp PCR fragment spanning the AAVS1 site ...
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bioRxiv - Cell Biology 2020Quote: Jurkat T cells (validated by SSTR method present 88% of homology with Jurkat ACC 282 with DSMZ website), and Raji B (ATCC, CCL-86) cells were cultured at 37 °C 5% CO2 in RPMI 1640 Glutamax (Gibco, 61870-010) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... Pictures of the infiltrated leaves were taken under UV illumination at 365 nm using a UVP Blak-Ray B-100Y UV lamp (Thermo Fisher Scientific) to detect the expression of the GFP reporter ...
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bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
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bioRxiv - Immunology 2020Quote: ... At day 4 and day 10 of culture B cells were mixed with at least 2000 Cyto-Cal counting beads (Thermo Fisher Scientific) or CountBright Absolute counting beads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... or 6 dpf zebrafish from one linc-mipep-/- heterozygous incross (for single-cell Multiome, n=12 per sample) in ice-cold Neurobasal media supplemented with B-27 (Thermo Fisher Scientific), snap-frozen in a dry ice/methylbutane bath (to preserve nuclear structure) ...
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bioRxiv - Genomics 2022Quote: ... buffer B: 0.1% formic acid in acetonitrile) on a 50cm μPAC™ column (PharmaFluidics) mounted on an EASY-Spray™ source (Thermo Scientific) connected to an Orbitrap Fusion LUMOS (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was digested with PstI and XbaI and ligated into the PstI–XbaI site of the pPICZα B vector (Thermo Fisher Scientific), which encodes a secretion signal of Saccharomyces cerevisiae α-factor ...
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bioRxiv - Molecular Biology 2023Quote: ... was performed on an Orbitrap Exploris 480 (related to Figure S2A and B) or an Orbitrap Fusion Lumos mass spectrometer (related to Figure 4E and F) (Thermo Fisher Scientific) connected to a Thermo Ultimate 3000 RSLCnano pump equipped with a self-pulled analytical column (150 mm length × 100 μm i.d. ...
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bioRxiv - Plant Biology 2022Quote: ... the induction media was removed by centrifugation and proteins were extracted by the resuspension of bacteria in B-PER™ Bacterial Protein Extraction Reagent (ThermoFisher Scientific) with 0.8 U/mL DNase I (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: The MT-B and MT-U alone data were collected at the Yale ScienceHill-Cryo-EM facility using a Glacios microscope (Thermo Fisher Scientific) operated at 200 keV ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 μM of rocaglate (silvestrol, RocA, zotatifin, or CR-1-31-B) and 75 μM SYPRO Orange (S6650, Invitrogen, Carlsbad, CA, USA) in a 20 mM HEPES-KOH buffer (pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Accutase was removed and the cells were then resuspended in DMEM with 2% B-27-supplemented 1% FBS and 1% PSF (Thermo Fisher Scientific) and plated on PDL/Laminin-2020-coated culture plates.
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bioRxiv - Systems Biology 2024Quote: ... cells were cultured for 72h with 10 nM SB431542 in culture media containing DMEM/F12 with 1x GlutaMAX/1x insulin-transferrin- selenium/1x NEAA/2% B-27 (Gibco 17504-044)/90 µM 2-Merceptoethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0, ThermoFisher Scientific). For the data analysis ...
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bioRxiv - Genetics 2023Quote: ... the medium was removed and NIM was added with doxycycline plus 5 µg ml–1 puromycin and 250 µg ml–1 hygromycin B (Thermo Fisher Scientific) (NIM selection medium) ...
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bioRxiv - Molecular Biology 2024Quote: ... over 60 min and 28% to 45% B over 13 min at 350 nl/min delivered by an Easy1200 nLC system (Thermo Fisher Scientific) with a total 90 min method length.
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bioRxiv - Neuroscience 2024Quote: ... using a 90 min gradient with a 250 nl/min flow rate of increasing Buffer B concentration (from 2% to 60%) on a High-Performance Liquid Chromatography (HPLC) system (Thermo Fisher Scientific), ionized with electrospray ionization (ESI ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells with appropriate genomic integrations in the Flp-In locus were selected using 100 µg/mL hygromycin B (10687010, Thermo Fisher Scientific) and 10 µg/mL blasticidin S (R21001 ...
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bioRxiv - Microbiology 2024Quote: ... and contrasted growth conditions (glucose and chitin) were resuspended in 175 μL 50 mM TEAB buffer (with 1% NaDOC) and 175 μL B-PER buffer (Thermo Scientific, Germany) by vortexing ...
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bioRxiv - Physiology 2023Quote: ... IALVs were then washed with PBS containing 0.1% Triton X-100 (PBST) 3 times and blocked for a minimum of 2 hr with Blockaid® (B-10710, ThermoFisher Scientific). IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution ...
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bioRxiv - Synthetic Biology 2023Quote: ... containing 100 or 500 µg/ml zeocin or YPD (YPDS without sorbitol) containing 200 µg/ml hygromycin B (Invitrogen, Thermo Fisher Scientific) already after 2 hours of incubation in 1 M sorbitol ...
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bioRxiv - Bioengineering 2023Quote: ... for 48 h at 4 °C each with a final step in rhodamine solution (4.17 mg/mL−1 Rhodamine B, pure in 100% ethanol, ACROS Organics, Geel, Belgium), followed by a xylene washing ...
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bioRxiv - Cell Biology 2023Quote: ... Flp-In T-REx 293 stable lines expressing FLAG-BirA* or FLAG-BirA* fusion TBCCD1 were grown as mentioned above after the addition of hygromycin B (200 μg/ml) (Invitrogen, Paisley, UK). The hTERT RPE-1 (RPE-1 ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Immunology 2023Quote: ... Neurons were seeded into PLL-coated cell culture treated plates and grown in Neurobasal Plus + B-27 supplement medium (Thermo-Fisher Scientific, #A3582901) prior to use in experiments 7-9 days in vitro (DIV) ...
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bioRxiv - Cell Biology 2023Quote: ... were cotransfected with pFRT-B-GRPA34 (Supplementary Table 2) (encoding GFP-RPA34) and pOG44 Flp-recombinase using Neon transfection (Cat.No.: MPK5000, Invitrogen, Waltham, Massachusetts, USA). Four hours after transfection the cell culture medium was exchanged and cells were grown for 48 h and selected with 2.5 mg ml-1 blasticidin (Cat.No. ...
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bioRxiv - Neuroscience 2023Quote: ... and/or pdcDNA-FlagMyc (B. Janssens, BCCM/LMBP Plasmid Collection, clone LMBP 4705, Zwijnaarde, Belgium) using LR Clonase II (Thermo Fisher Scientific). More details of the plasmids and primers used are in supplementary tables S1 and S2 ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were screened for roller progeny and positive plates were treated with approximately 200-300 μl of hygromycin B (20 mg/mL) (Thermo Fisher Scientific). Hydromycin-resistant roller worms were singled to individual plates to assess roller progeny ...
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bioRxiv - Biochemistry 2022Quote: ... 1×105 cells resuspended in PBS-B were added per well of a 96-well plate (V-bottom, non-treated surface; Thermo Fisher Scientific) and kept on ice ...
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bioRxiv - Cell Biology 2023Quote: Overlay of EM and LM data (Fig. 3) and most of the segmentations (Fig. 4A,B,D,E) were done using Amira (Thermo Fisher Scientific). Additional segmentation was performed using Microscopy Image Browser (Belevich et al ...
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bioRxiv - Cancer Biology 2023Quote: ... at a density of 1.0 × 105 cells/mL in corresponding culture media supplement with 1X B-27 (Life Technologies, Cat: 17504-001), 20 ng/mL human recombinant bFGF (StemCell ...
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bioRxiv - Biochemistry 2023Quote: ... We tested Zeocin-resistant clones that had a single integration site detected by Southern blot for high-activity integration sites by using the mammalian b-galactosidase activity assay (Gal-Screen, Thermo Fisher Scientific). Clonal expansion of the selected colony established the HeLa-FRT cell line ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Biophysics 2023Quote: ... chemiluminescence was recorded at the FlexStation3 after 10 min incubation with substrates A and B of SuperSignal West Pico PLUS kit (Thermofisher, ref: 34577). Average values from 3 replicates were normalized to WT HA3-CXCR4 expression.