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Citations for Thermo Fisher :
2301 - 2350 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing the virus was then used fresh or stored by snap-freezing aliquots supplemented with 8 μg/ml polybrene (Fisher Scientific) in ethanol/dry ice and stored at -80° C ...
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bioRxiv - Biophysics 2022Quote: ... SOFI raw data was obtained using a buffer containing: 200 µl buffer C complemented with 8 mM KI (CAT: 373651000 - Thermo Scientific) and 300 mM MgCl2 (Product nr ...
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bioRxiv - Developmental Biology 2022Quote: ... Fly ovaries from 2-to 8-day old females were dissected in Schneider’s Drosophila Medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Seradigm FBS ...
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bioRxiv - Immunology 2022Quote: ... Cell debris was removed from lysate containing soluble protein by centrifugation at 35,000 x g and 4 °C for 45 min (Sorvall LYNX 6000 centrifuge, F21-8×50y rotor, Thermo Fisher Scientific). Proteins were further purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Evolutionary Biology 2022Quote: ... After adding 8 μL of the master mix to wells of a 384-well MicroAmp Optical Reaction Plate (Applied Biosystems 4309849), 2 μL of genomic DNA sample which had been diluted 2:1 with nuclease free water was added ...
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bioRxiv - Plant Biology 2022Quote: ... T-DNA insertion position was confirmed with gene-specific and T-DNA-specific primers (Supplemental Table 8) with Phire Plant Direct PCR Master Mix (Thermo Scientific) according to the manufacturer’s guidelines.
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bioRxiv - Microbiology 2022Quote: ... were loaded and separated on 8% and 12% SDS-PAGE gel and transferred onto a polyvinylidene difluoride transfer membrane (Thermo Fisher). Immunoblotting was performed by using the corresponding antibodies ...
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bioRxiv - Microbiology 2022Quote: Proteins (8 μM final) were incubated with 0.16 mM of (2'-(or-3')-O-(trinitrophenyl) adenosine triphosphate (TNP-ATP) (Thermo Fisher Scientific), in 50 μl of Binding Buffer (10 mM HEPES-KOH (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... and diluted to a final concentration of 8 × 104 cells/μL in sterile 1x PBS (pH 7.4, without calcium or magnesium; Thermo Fisher Scientific). Cells were stored on ice until transplantation ...
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bioRxiv - Microbiology 2023Quote: Transfection studies were conducted in HCT-8 and HeLa cells by the standard transfection protocol employing reagents lipofectamine 2000 and lipofectamine LTX (Invitrogen, USA) respectively ...
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bioRxiv - Physiology 2023Quote: ... 8 mg of the soluble protein fractions were pre-cleared with 200 μL of protein G-magnetic Dynabeads (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... the midbrain or cortical tissue from the 6-8 embryos were pooled together before digestion with Trypsin-EDTA 0.12% (Life Technologies, USA) for 7 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 2-3 mg/mL proteins were applied to glow-discharged M026-Au300-R20/20 Copper grid (CryoMatrix) and blotted for 3 s at 8 ℃ with 100% humidity and plunge-frozen in VitroBot Mark IV (Thermo Fisher).
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bioRxiv - Molecular Biology 2022Quote: ... MF002 - plus-10) and the PCR products were fractionated on 8% TBE gel stained with SYBR Gold nucleic acid stain (Invitrogen, S11494). Quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2022Quote: ... DNA concentration was quantified using Qubit and the quality of DNA was visualized using 8% TBE gel (Invitrogen, Cat. No. EC62152BOX) performed at 230 V for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Differentiated iNeurons were then cryogenized as aliquots of 1 · 106 to 8 · 106 cells in heat inactivated fetal bovine serum (FBS, 10270-106, Thermo Fisher) containing 5% v/v dimethyl sulfoxide (DMSO) ...
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Simultaneous adjunctive treatment of malaria and its co-evolved genetic disorder sickle cell anaemiabioRxiv - Microbiology 2022Quote: ... Serial dilutions of Pf3D7 total RNA and DNA (ranging from 8 to 5000 pg/µl) with nuclease-free water (AM9935, Life Technologies) were used to generate a relative standard curve for pfr1 ...
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bioRxiv - Cell Biology 2022Quote: Dictyostelium discoideum cells were maintained in Hans’ enriched HL-5 media (1.4X HL-5 media with 8% FM, penicillin and streptomycin) at 22°C in petri dishes (Fisher Scientific; FB0875712). A full list of strains used in this study can be found in Appendix Table S3.
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bioRxiv - Microbiology 2023Quote: Experiments were conducted in solution with an initial composition of either 8 g/l (0.14 molal (m)) NaCl solution (ThermoFisher Scientific, 207790010), 5.8 g/l (0.14 m ...
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bioRxiv - Plant Biology 2024Quote: ... the genomic DNAs of DUF760-1 and 2 (without the stop codon) were cloned with specific primers (Supplemental Table 2) into the Gateway compatible vector pCR™8/GW/TOPO (Invitrogen) and then transferred to the vector pGWB540 with the YFP at the C-terminus using LR clonase (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were grown with DMEM/F12 containing 10% FBS on 8-well Lab-Tek chamber slides (Nunc Lab-Tek Chamber Slide System, Thermo Scientific) precoated with 0.1% gelatin in PBS overnight ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 µL of the reaction were then denatured in Laemmli buffer and run on a 8-16% polyacrylamide gel by SDS-PAGE and stained with either Brilliant Blue R250 or Coomassie Fluor Orange (Molecular Probes) and visualized on a Bio-Rad ChemiDoc MP imager.
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Molecular Biology 2024Quote: ... All Expi293F cells were cultured at 8% CO2 saturation and 37°C with shaking in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 4 mM L-glutamine.
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bioRxiv - Neuroscience 2024Quote: Astrocytes were FACS sorted as described above and plated in 8-well Lab-Tek II®CC2 Slide (Thermo Scientific, 1054941). Within 2-3 days after plating ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to 8% SDS-PAGE followed by Western blotting using iBlot 2 Dry Blotting System (Thermo Fisher Scientific). 1.0 μg/ml rabbit polyclonal anti-nucleolin antibody (ab22758 ...
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bioRxiv - Cell Biology 2024Quote: ... to form condensates (with 20% v/v PEG 8 kDa as needed) and overlaid with fluorescent dextrans of various sizes at 10 kDa (Invitrogen D1816), 40 kDa (Invitrogen D1842) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... of the homogenized protein-loading buffer sample were loaded in duplicates on a NuPAGE™ 3-8% Tris-Acetate gel (ThermoFisher) to be probed separately with FLAG and ATPase antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... Then the tubes were transferred into ice and added with 8 μL 5X First-Strand Buffer of SuperScript™ III RT kit (Invitrogen), 2 μL of 0.1 M DTT ...
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bioRxiv - Microbiology 2023Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher, catalog #A33952), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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bioRxiv - Genomics 2024Quote: ... Protein extracts were subjected to electrophoresis in 10% polyacrylamide SDS gels using Tris-glycine-SDS running buffer (Alaos) or in NuPAGE 3-8% Tris-acetate precast polyacrylamide gels (Thermo Fisher), with Tris-acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... All bacterial stocks were stored as aliquots at -80°C in brain heart infusion media (BHI; Fisher Scientific, DF0037-17-8) to minimize freeze-thaw cycles ...
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bioRxiv - Immunology 2024Quote: ... cleanup beads were used (0.8:1 beads/sample ratio) and then eluted in 10 µL of DNase/RNase-Free Distilled Water (10977- 049, ThermoFisher®). cDNA was quantified by the Quant-iT PicoGreen dsDNA Assay Kit (P7581 ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen rat ovarian follicles were homogenized in 400 µL lysis buffer (100 mM HEPES, pH 8, 4% SDS, 1 mM EDTA, and 1× Halt protease/phosphatase inhibitor cocktail (ThermoFisher Scientific) using Zirconia/glass beads in a bead beating grinder (FastPrep24 ...
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bioRxiv - Bioengineering 2024Quote: Knock-in percentage and cell counts were measured on day 10 of culturing (day 8 post-electroporation) by flow cytometry using the Attune NxT flow cytometer with a 96-well autosampler (ThermoFisher Scientific). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Human embryonic stem cell line H9 was cultured on the 6-well plate coated with the Vitronectin (5 μg/ml) and in the Essential 8 Flex medium (both Thermo Fisher). Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher ...
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bioRxiv - Biophysics 2023Quote: The cysteine-bearing Aβ40 variant was redissolved in 50 mM NaPi pH 7.5 and labelled with an 8-fold molar excess of Alexa Fluor™ 488 C5 Maleimide (AF488; Invitrogen Life Technologies) and incubated at room temperature for 2 h (Supplementary Figure 3) ...
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bioRxiv - Biophysics 2023Quote: ... and U-251 cells were seeded onto PLL-PEG-RGD-coated 8-well chambers in the respective medium with penicillin (1 unit/mL) and streptomycin (1 µg/mL; Gibco, Life Technologies) at densities between 0.5 x 104 to 2.5 x 104 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed once with DMEM/F-12 and then cultured in Glasgow’s MEM (WAKO) containing 8% Knockout Serum Replacement (KSR, Thermo Fisher Scientific), 1 mM sodium pyruvate (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... 14 min (100SPD) or 8 min (180SPD) total runtime with PepMap™ columns 150 μm x 15 cm (Thermo Fisher Scientific) in a trap&elute configuration ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... monolayers and Vero (African Green Monkey Kidney Cell) cell lines were cultured with 10% and 8% fetal bovine serum (FBS, Gibco, USA) DMEM (Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Neuroscience 2023Quote: ... To revive hiPSCs from cryopreservation cells were thawed by incubation for 2 minutes at 37°C and retrieved by centrifugation (200 x g for 3 minutes) prior to resuspension in Essential 8 (E8, (A1517001, Life Technologies) medium with 1’RevitaCell ...
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bioRxiv - Immunology 2023Quote: ... Vesicles were subjected to high-speed centrifugation for 4 h at 55,000 g (25°C) and subsequently at 8 h at 75,000 g (4°C) with an ultracentrifuge (Sorvall RC M150GX, Thermo Fisher Scientific) using a fixed angle rotor (S150AT-0128 ...
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bioRxiv - Molecular Biology 2023Quote: Flp-In T-REx HeLa cells of the genotypes indicated in the figure legends were plated in 8-well Lab Tek II Chamber 1.5 German coverglass dishes (Thermo Fisher, 155409) in regular growth medium ...
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bioRxiv - Cell Biology 2023Quote: ... or grown into a monolayer in Nunc™Lab-Tek™ chambered #1.0 Borosilicate Coverglass System 8 chambers microscopy dish (ThermoFisher). cbBOECS were imaged in EGM-18 medium at 37°C and 5% CO2.
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bioRxiv - Pathology 2023Quote: ... The explants were cut (1.0 cm2) and cultured at an air-liquid interface on culture inserts (filter pore size 8 μm; #141082, Thermo Fisher Scientific) with a 1:1 mixture of Ham’s F-12 and DMEM supplemented with 0.3% FCS ...