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Citations for Thermo Fisher :
2101 - 2150 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...
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bioRxiv - Neuroscience 2024Quote: Total protein from whole cell lysates of mPFC and NAc (6-8 µg) were loaded onto NuPAGE 4-12% Bis-Tris gels (Invitrogen) under reduced and denatured conditions (Pierce 4X LDS sample buffer ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...
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bioRxiv - Bioengineering 2024Quote: ... 200 μl of antibody or scFv at 1 mg/ml in PBS was mixed with 10% volume of 1M sodium bicarbonate to adjust pH to 7-8 followed by addition of EZ-Link sulfo-NHS-LC-Biotin (ThermoFisher) to reach a 5:1 biotin:protein molar ratio ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellets were resuspended in 500 μl of 8 mg/ml dithiobis (succinimidyl propionate; DSP) solution (Thermo Fisher Scientific) and cross-linked ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were centrifuged from the culture medium and resuspended in an equal amount of Fluo-8 dye-loading solution along with Hank Balanced Salt Solution (HBSS buffer) supplemented with calcium (Gibco). A suspension containing 200,000–250,000 P2X3-Expi293 GnTI- cells per well was prepared in a 96-well poly-D-lysine plate ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: HEK293T/17 (ATCC #ACS-4500) and HCT-8 (ATCC #CCL-244) cells were cultured in Dulbecco’s modified Eagle medium (DMEM; ThermoFisher #11995065) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) and 8 µg/mL of blasticidin (all Gibco brand, from Thermo Fisher Scientific). The SH-SY5Y cell line was cultured in the minimum essential medium Eagle (EMEM ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) and 8 µg/mL of blasticidin (all Gibco brand, from Thermo Fisher Scientific). The SH-SY5Y cell line was cultured in the minimum essential medium Eagle (EMEM ...
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bioRxiv - Molecular Biology 2024Quote: hTERT-RPE1 cells carrying an inducible copy of GFP multimers and FlpIn T-Rex 293 cells carrying an inducible copy of 3×FLAG-SNAP-SAF-A were grown in 8-well Lab-Tek II Chambered Coverglass -- 1.5 borosilicate glass (Thermofisher scientific).
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bioRxiv - Biochemistry 2024Quote: ... followed by an enzyme solution consisting of 8 nM wild-type PETase or its mutant labeled with 0.8 nM Qdot655 (Thermo Scientific), 2 mM 2-Mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... alongside control human embryonic stem cell (CTRL-ES) and FMR1 knock out line (FMR1KO) used in this study were maintained in Essential 8 Medium (E8, Gibco) in plates coated with Matrigel Growth Factor Reduced Basement Membrane Matrix and were passaged at 80% confluence with 1 mg/ml collagenase and 0.5 mg/ml dispase (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of cRNA was used for reverse transcription with 2 pmoles of a 16S rRNA-specific primer (Supplementary Table 8) and SuperScript II (ThermoFisher), following the manufacturer’s instructions ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... After neutralization with 1/10 volume of 1 M sodium phosphate buffer (pH 8) the antibody was coupled to M270 Epoxy Dynabeads (Thermo Scientific) in ammonium sulfate buffer (0.1 M sodium phosphate pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cancer Biology 2021Quote: Cells stably expressing V5-tagged XPR1 and KIDINS constructs were plated at a density of 10,000 -20,000 cells per well in NuncTM Lab-Tek™ II CC2™ 8-well Chamber Slides (Thermo Fisher). When investigating localization upon knockdown ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from 8/16-cell embryos (10-15/group/replicate) was isolated using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems). Purified RNA was then subject to reverse transcription with SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue was then transferred to 1% fibronectin-coated chambered slides (μ-Slide 8 Well Glass Bottom, Ibidi #80827, for live imaging; Nunc™ Lab-Tek™ II Chamber Slide with removable wells ...
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bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2020Quote: ... embryos were washed 8 × 15min in PBST (PBS with 0.1% Tween-20) and incubated in Image-iT FX Signal Enhancer (ThermoFisher Scientific, I36933) for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen, Cat. No EA03752BOX). Transfer onto nitrocellulose membranes was performed using the iBlot 2 Dry Blotting System (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific, C10337). For induction of apoptosis in myoblasts ...
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bioRxiv - Microbiology 2021Quote: ... was seeded with influent solids harvested from the influent of the RAEBL full-scale WRRF by weekly centrifuging 10 L of a 24-h composite influent sample at 4,000×g using the IEC-Centra-8 centrifuge (Thermo Scientific, USA). The total suspended solids (TSS ...
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bioRxiv - Neuroscience 2020Quote: Human iPSCs were maintained as feeder-free cells in Essential 8 medium on vitronectin-coated plates (all from Gibco, Carlsbad, CA) (69) ...
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bioRxiv - Synthetic Biology 2022Quote: HEK:BFP cells were the kind gift of Chris Richardson.8 Cells were maintained at 37°C and 5% CO2 in DMEM media plus GlutaMax (ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 7 days and 14 days post SNI) L3 and L4 DRGs were dissected from 2 mice (8 DRGs/sample) and stored in ice-cold HBSS (Gibco, 14170088). DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase ...
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bioRxiv - Neuroscience 2021Quote: ... or 13C6 L-Lysine-2HCl (Lys-8) and 13C6 L-Arginine-Hcl (Arg-10) for the metabolic incorporation of ‘light’ and ‘heavy’ stable isotopes respectively (ThermoFisher Scientific). HEK293FT cells were grown in light or heavy SILAC media for at least 10 doublings ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were centrifuged again (80 g, 8 min) and suspended at the desired concentrations in culture medium (Neurobasal medium (Thermofisher 21103049) supplemented with 1 % GlutaMAX (Thermofisher 35050) ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate volume was increased to 1 ml and 8 μg of antibody were used in combination with Protein G Dynabeads M-280 (Thermo Scientific) as described above ...
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bioRxiv - Molecular Biology 2020Quote: Flash frozen MCAs with surrounding brain tissue were sliced to 8 μm sections using a cryotome (Fisher Scientific, Pittsburgh, PA, USA), placed on charged slides (2-4 slices/slide) ...
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bioRxiv - Molecular Biology 2020Quote: INS1 cells were re-plated onto a cover slip as above and incubated in regular INS1 medium containing 8 μM Bodipy C12 (Molecular Probes) at 37 °C at 5% CO2 for the last 16 h of culture ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were transfected with indicated plasmids alone or in combination for 8 h by using Lipofectamine 3000 (Thermo Fisher, USA), and then continue to cultivate for 24 h in the fresh medium before being subjected to indicated experiments.
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bioRxiv - Molecular Biology 2020Quote: ... and 10-20 µg of protein loaded on a 3%–8% NuPAGE Tris-Acetate Gel in tris-acetate buffer (Novex Life Technologies) or a 4-12% NuPAGE TrisBis NuPAGE gels in MES buffer (Novex Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Each CDM pellet was resuspended in 100 mM NH4HCO3 solution containing 8 M urea and 10 mM dithiothreitol (DTT; Thermo Fisher) and incubated at 37 °C for 2 hours under agitation ...
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bioRxiv - Cell Biology 2020Quote: ... 25 µL drops of cell-Matrigel suspensions were seeded into each well of an 8-well chambered coverglass (Nunc, No. 1.5) and incubated at 37 °C for 30 minutes before adding DMEM to cells ...
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bioRxiv - Immunology 2021Quote: ... and checked for purity and quality on a silver-stained SDS-PAGE gel (3-8% Tris-Acetate Novex, Thermo Fisher Scientific). The anti-NTD neutralizing antibody NTD-18 and the pan-coronavirus anti-S2 non-neutralizing antibody Ab-10 were previously described 9,10.
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bioRxiv - Cancer Biology 2019Quote: ... HEK293T cells in a 10 cm dish with about 60% confluence were transfected with 8 μg lentiviral construct and helper plasmids using Lipofectamine 2000 (Life Technologies). 24 hours after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... for NIH-3T3 cells and P19 cells or 5nM siRNA/8 µl INTERFERin (PolyPlus) for MDA-MB-231s and 200 µl Opti-MEM (ThermoFisher Scientific) in fresh media ...
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bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA produced from 15 ng RNA and 8 pmol of each forward and reverse primer per reaction and Power SYBR Green PCR Master Mix (Applied Biosystems) were used ...
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bioRxiv - Pathology 2019Quote: PMLECs between the 3rd and 5th passages were seeded (8 × 104/well) in 2-well Permanox chamber slides from Lab-tek (Thermo Scientific, Nunc). The cells were incubated at 37°C and 5% CO2 for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested before the cultures reached OD600 = 1 and were labeled with 8 mg/ml EZ-Link Sulfo-NHS-LC-biotin (ThermoFisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Systems Biology 2019Quote: ... Human ESCs (WA-01; National Institutes of Health registry #0043) were maintained in Essential 8 medium (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin-amphotericin B solution (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The homogenate was centrifuged at 10,000g for 15 minutes (Sorvall™ ST 8 Small Benchtop Centrifuge: Thermo Scientific, Waltham, MA, USA). The supernatant was used as the enzyme source ...
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bioRxiv - Molecular Biology 2019Quote: Human atherosclerotic plaque or mouse aortic root specimens were first cut into 8 μm sections using a freezing microtome (Thermo Scientific). For immunofluorescence detection to detect Tf ...
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bioRxiv - Developmental Biology 2019Quote: ... One-third to one embryo equivalents were loaded at per lane on 4%-12% or 8% Bolt® Bis-Tris (Invitrogen). GFP Rabbit IgG Polyclonal Antibody (Molecular Probes ...
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bioRxiv - Biophysics 2019Quote: ... were plasma-cleaned for 10 minutes in an Harrick Plasma Cleaner (Harrick) and attached to 8-well LabTek chambers (Nunc, USA), for which the bottom glass slide had been removed ...
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bioRxiv - Genetics 2019Quote: Xenograft mouse model of human lung cancer tumors were implanted under the left upper limb in the abdomens of 6- to 8-week old male NCG mice by subcutaneous injection of A549 (5×106 cells in 200 µL DPBS (Gibco, C14190500BT)) or H2228 cells (2× 106 cells in 200 µL DPBS) ...