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Citations for Thermo Fisher :
2201 - 2250 of 2644 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation (19 000 rpm for 45 min at 4°C in a Fiberlite F21-8×50y (Thermo Scientific). Supernatant was incubated with 5 ml Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... EBs were grown in suspension for 7-8 days followed by adherence to Matrigel-coated plates in the Neural Induction Medium (NIM) consisting of DMEM/F12 (GIBCO, 11320033), 2⍰mM l-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... WTC11 iPSCs were cultured and expanded in six-well plates using Essential 8 media (Gibco/Thermo Fisher Scientific, Cat. No. A1517001). Once iPSCs were confluent ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs expressing six inducible transcription factors (MAFB, CEBPα, IRF, PU1, CEBPβ, IRF5) were grown in Essential 8™ Basal Medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Single-cell isolated from day 8 organoids (200 unselected organoids) were incubated for 5 minutes at room temperature with Calcein-AM (C3099, Life Technologies) and subsequently sorted on a FACS Aria (BD ...
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bioRxiv - Developmental Biology 2023Quote: Cells were thawed according to the provider’s protocol and the medium was gradually changed to Essential 8 Flex Medium (E8f) (A2858501 Thermo Fisher / Life Technologies) supplemented with 100 μg/ml Normocin (4069-ant-nr-1 ...
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bioRxiv - Bioengineering 2023Quote: ... Washed cells were resuspended in 150 μL of FACS buffer and treated with anti-mouse MHC class I antibody conjugated to PE (Clone:28-14-8) (Invitrogen, USA) at a concentration of 2μg per sample and anti-mouse MHC class II antibody conjugated to FITC (Clone ...
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bioRxiv - Immunology 2023Quote: Purified CD11c+ myeloid cells (5 × 104 cells) from bone marrow cultures were plated on an 8 well glass chamber slide (Nunc Lab-Tek-Thermo Fisher) in supplemented IMDM medium and incubated for 24 h ...
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bioRxiv - Biochemistry 2023Quote: ... Freshly isolated mitochondria were resuspended in 50 μl of click-it lysis buffer (50 mM TRIS-HCl pH 8, 1% SDS, 250 U/mL Universal Nuclease [Thermo Fisher]) and incubated on ice for 15 min ...
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bioRxiv - Biophysics 2023Quote: ... 8-μm-thick cryosections were then cut with a Microm Cryo Star HM 560 cryostat (Thermo Fisher Scientific, Waltham, MA, USA) and mounted on microscope slides (Superfrost UltraPlus ...
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bioRxiv - Microbiology 2023Quote: Human ileocecal adenocarcinoma cells (HCT-8; ATCC CCL-244) were cultured in RPMI 1640 ATCC Modification medium (Thermo Fisher A14091-01) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were cultured to confluence and passaged 4 times before plating to 8-chamber slides (Thermo Fisher Scientific Cat#177445) coated with Matrigel (Thermo Fisher Scientific Cat# CB-40230 or Corning Cat# 356230 ...
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bioRxiv - Biochemistry 2023Quote: ... 24 hours after seeding 8×103 HeLa cells in 100 µl media per well of tissue-culture treated 96-well plates (ThermoFisher, 167008), the cells are treated with 9-point ...
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bioRxiv - Biophysics 2023Quote: ... against PIEZO1 that targets the 5’-AGCATTGAAGCGTAACAGGG-PAM-3’ at Chr.8: 122513787 - 122513809 on GRCm38 was purchased from Invitrogen (A35533). The cells were transfected with the Cas9 enzyme (TrueCut Cas9 Protein v2 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then resuspended in Essential 8 medium containing 20 μM Ri and counted using the Countless cell counting chambers slides (Invitrogen, C10313). For condition C4 ...
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bioRxiv - Cell Biology 2023Quote: ... WT and UBAP2L KO HeLa cells were grown on 8-well Chambered Coverglass w/non-removable wells (Thermo Fisher Scientific, 155411PK) and transfected with the reporter plasmid XRGG-GFP for 30h ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs (EDOM22 #8) derived from menstrual blood were used.53,54 The iPSCs were cultured on dishes coated with vitronectin (A14700, Thermo Fisher Scientific) in Stem flex medium (A3349401 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with plasmid DNA (0.5 µg per well of a 24-well plate and 0.25 µg per chamber of an 8-well chamber slide unless stated otherwise) 24 h post-plating using Lipofectamine LTX (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and cultured at 37°C in a humidified 8% CO2 incubator with constant rotation at 130 RPM using Expi293 Expression Medium (ThermoFisher Scientific). DNA transfections were conducted using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by sodium dodecyl sulfate/polyacrylamide (SDS/PAGE) gel electrophoresis on 1 mm NuPAGE 3 – 8% gradient Tris-Acetate gels (Thermo Fisher) using Tris-Acetate SDS running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal quantities (20-50 μg) of samples were heated at 95 degrees for 8 minutes in 1X LDS buffer with 1X reducing agent (Thermo Fisher). Samples were then separated by SDS-PAGE and transferred onto an a PVDF membrane (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: Adult DRG from adult C57BL/6J mice (8-10 weeks old) were rapidly dissected and placed in ice-cold DMEM/F12 (Gibco 11330057). DRG were washed 3 times with ice-cold calcium- and magnesium-free HBSS (Gibco 14175095 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded into 6-well plates 24 h prior to transfection, 50 nM siRNA and 8 μL INTERFERin (Polyplus, France) were mixed in Opti-MEM (Gibco, USA), incubated for 10 min at room temperature and added to the cells ...
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bioRxiv - Molecular Biology 2023Quote: ... feeder-free mouse ESCs were grown as aggregate culture for eight days (DIV-8) in differentiation media consisting of Glasgow’s MEM (GMEM) (Thermo Fisher Scientific) supplemented with 5% Knockout Serum Replacement (KSR ...
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bioRxiv - Biophysics 2023Quote: ... Lysates were clarified by centrifugation at 13,000 rpm for 20 min in a Sorvall RC6+ centrifuge using a F21S-8×50y rotor (Thermo Fisher Scientific) at 37°C and incubated with 0.5 mL of Ni–NTA beads (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS and subsequently incubated for 2 hours with fresh culture media containing 10 μL of CCK-8 solution (catalog no: NC9261855, Fisher Scientific) added in each well ...
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bioRxiv - Cancer Biology 2023Quote: Untreated MCF-7 and MDA-MB-231 cells were seeded at 2 x 104 cell per well on 8-well glass chamber slides (™ Nunc™ Lab-Tek™ II Chamber Slide™ ...
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bioRxiv - Cancer Biology 2023Quote: Clear lysates from cells and EVs in 8M urea buffer (8 M urea, 75 mM NaCl, 50 mM Tris) were quantified using microBCA Protein Assay Reagent Kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated twice for 30 s and then cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Amylose beads (Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were resuspended in 2 mL of 8 M urea (containing 50 mM HEPES, pH 8.5) and protein concentrations were measured by BCA assay (Thermo Fisher Scientific) before protease digestion ...
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bioRxiv - Cell Biology 2023Quote: HeLa wild-type or HeLa POLE4 KO cells were grown in 8-well Lab-Tek II chambered coverglass 30 (Thermo Scientific) and transfected 48 h prior to imaging with GFP-tagged PARP1 chromobody (Chromotek) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Binding of compound to plasma proteins was determined by equilibrium dialysis of 3 µmol/L compound in plasma or serum against PBS through an 8 kDa molecular-weight cut-off cellulose membrane (RED device, Thermo Fisher) at 37 °C for 5 h ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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bioRxiv - Genomics 2024Quote: ... Protein extracts were subjected to electrophoresis in 10% polyacrylamide SDS gels using Tris-glycine-SDS running buffer (Alaos) or in NuPAGE 3-8% Tris-acetate precast polyacrylamide gels (Thermo Fisher), with Tris-acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... All bacterial stocks were stored as aliquots at -80°C in brain heart infusion media (BHI; Fisher Scientific, DF0037-17-8) to minimize freeze-thaw cycles ...
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bioRxiv - Immunology 2024Quote: ... cleanup beads were used (0.8:1 beads/sample ratio) and then eluted in 10 µL of DNase/RNase-Free Distilled Water (10977- 049, ThermoFisher®). cDNA was quantified by the Quant-iT PicoGreen dsDNA Assay Kit (P7581 ...
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bioRxiv - Biochemistry 2024Quote: ... 14 min (100SPD) or 8 min (180SPD) total runtime with PepMap™ columns 150 μm x 15 cm (Thermo Fisher Scientific) in a trap&elute configuration ...
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bioRxiv - Microbiology 2023Quote: Experiments were conducted in solution with an initial composition of either 8 g/l (0.14 molal (m)) NaCl solution (ThermoFisher Scientific, 207790010), 5.8 g/l (0.14 m ...
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bioRxiv - Plant Biology 2024Quote: ... the genomic DNAs of DUF760-1 and 2 (without the stop codon) were cloned with specific primers (Supplemental Table 2) into the Gateway compatible vector pCR™8/GW/TOPO (Invitrogen) and then transferred to the vector pGWB540 with the YFP at the C-terminus using LR clonase (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were grown with DMEM/F12 containing 10% FBS on 8-well Lab-Tek chamber slides (Nunc Lab-Tek Chamber Slide System, Thermo Scientific) precoated with 0.1% gelatin in PBS overnight ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 µL of the reaction were then denatured in Laemmli buffer and run on a 8-16% polyacrylamide gel by SDS-PAGE and stained with either Brilliant Blue R250 or Coomassie Fluor Orange (Molecular Probes) and visualized on a Bio-Rad ChemiDoc MP imager.
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Molecular Biology 2024Quote: ... All Expi293F cells were cultured at 8% CO2 saturation and 37°C with shaking in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 4 mM L-glutamine.