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Citations for Thermo Fisher :
2351 - 2400 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... monolayers and Vero (African Green Monkey Kidney Cell) cell lines were cultured with 10% and 8% fetal bovine serum (FBS, Gibco, USA) DMEM (Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... WT and UBAP2L KO HeLa cells were grown on 8-well Chambered Coverglass w/non-removable wells (Thermo Fisher Scientific, 155411PK) and transfected with the reporter plasmid XRGG-GFP for 30h ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs (EDOM22 #8) derived from menstrual blood were used.53,54 The iPSCs were cultured on dishes coated with vitronectin (A14700, Thermo Fisher Scientific) in Stem flex medium (A3349401 ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Genetics 2023Quote: ... IP and input DNA were purified using ChIP elution buffer (250 mM NaCl, 1% SDS, 10 mM Tris-Cl pH 8, 1mM EDTA) and incubation with proteinase K (ThermoFisher Scientific) overnight at 65°C ...
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bioRxiv - Genomics 2023Quote: ... Immunoblotting was performed according to standard procedures with the following modifications: a 3-8% tris acetate gel (Thermo Fisher Scientific, EA03752) was used and samples were run for 2 hours 15min at 80V at 4 °C in 1X tris-acetate running buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed in 8 M urea and quantified using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). Samples were denatured in Laemmli buffer at 95 °C and run at 170 V on a 10% acrylamide gel with a 4% stacking gel ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were rinsed twice with PBS and lysed in 8 M urea 50 mM Tris pH 7.4 containing protease inhibitor (Thermo Fisher Scientific) and DTT ...
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bioRxiv - Cell Biology 2023Quote: ... the two solutions were rapidly mixed together with 15 pumps on the syringe handles and instantly deposited into a well in an 8-well Lab-Tek dish (Thermo Scientific). We sought to maintain similar collagen fiber architecture when IPN stiffness and viscoelasticity was tuned ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 cells were seed in each well of 8-well chambers coated with a thick layer of matrigel (Thermor Fisher Scientific) and covered with 200 ul full-medium containing 3% matrigel ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no. 150239, Thermo Scientific) and then cultured at 22°C until sexual maturity ...
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bioRxiv - Synthetic Biology 2023Quote: ... All primers and oligos were ordered from Integrated DNA Technologies (IDT) dried and resuspended in 10 mM Tris buffer (pH=8, Fisher Scientific).
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bioRxiv - Synthetic Biology 2023Quote: ... Soluble proteins were separated from cell debris by centrifugation in a fixed angle rotor (Thermo Fisher Scientific, Fiberlite F15-8×50cy) at 10,000 rpm for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: Human ileocecal adenocarcinoma cells (HCT-8) (ATCC) were grown on coverslips in DMEM supplemented with 10% cosmic calf serum (CCS) (Thermo Fisher). 200,000 purified oocysts were excysted and seeded on coverslips when HCT-8 cells were 80% confluent ...
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bioRxiv - Cell Biology 2023Quote: ... or grown into a monolayer in Nunc™Lab-Tek™ chambered #1.0 Borosilicate Coverglass System 8 chambers microscopy dish (ThermoFisher). cbBOECS were imaged in EGM-18 medium at 37°C and 5% CO2.
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bioRxiv - Pathology 2023Quote: ... The explants were cut (1.0 cm2) and cultured at an air-liquid interface on culture inserts (filter pore size 8 μm; #141082, Thermo Fisher Scientific) with a 1:1 mixture of Ham’s F-12 and DMEM supplemented with 0.3% FCS ...
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bioRxiv - Genomics 2023Quote: ... From this sample a cell clone was derived from primary cells isolated from bat kidney tissue and immortalized via the transfer and stable production of the Simian virus 40 Large T antigen (SV40LT). Kidneys were cut in small pieces (ca. 8 mm3) and incubated in 0.05% Trypsin-EDTA solution (Gibco # 25300-054) for 15 h at 4 ºC ...
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bioRxiv - Biochemistry 2023Quote: ... 24 hours after seeding 8×103 HeLa cells in 100 µl media per well of tissue-culture treated 96-well plates (ThermoFisher, 167008), the cells are treated with 9-point ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then resuspended in Essential 8 medium containing 20 μM Ri and counted using the Countless cell counting chambers slides (Invitrogen, C10313). For condition C4 ...
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bioRxiv - Bioengineering 2023Quote: ... The stable iPSCs expressing the CRISPR repressor were cultured in a xeno-free and feeder–cell-free state in Essential 8™ Flex Medium (Gibco) along with the addition of Essential 8™ Flex Supplement (50X ...
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bioRxiv - Cell Biology 2023Quote: ... a reducing and oxygen-scavenging buffer optimized for dSTORM blinking (Halpern, Howard, & Vaughan, 2015) comprising 100 mM Tris-HCl (pH 8, J22638-K2, Thermo Scientific), 10% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... the media was removed and the cells were incubated with 8 µM CellEventTM Caspase 3-7 green detection reagent (Thermo Fisher) in PBS containing 5% FBS for 30 min at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... the denatured nuclear extract was subjected to electrophoresis on a precast 3-8% Tris-Acetate polyacrylamide gel (Thermo Fisher Scientific, EA0378BOX) in 1× Tris-Acetate SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Whole cell and nuclear compartment were lysed with 8 M Urea buffer containing a protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) and were sonicated for 15 seconds at 60% amplitude ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation (19 000 rpm for 45 min at 4°C in a Fiberlite F21-8×50y (Thermo Scientific). Supernatant was incubated with 5 ml Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... EBs were grown in suspension for 7-8 days followed by adherence to Matrigel-coated plates in the Neural Induction Medium (NIM) consisting of DMEM/F12 (GIBCO, 11320033), 2⍰mM l-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... WTC11 iPSCs were cultured and expanded in six-well plates using Essential 8 media (Gibco/Thermo Fisher Scientific, Cat. No. A1517001). Once iPSCs were confluent ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs expressing six inducible transcription factors (MAFB, CEBPα, IRF, PU1, CEBPβ, IRF5) were grown in Essential 8™ Basal Medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Single-cell isolated from day 8 organoids (200 unselected organoids) were incubated for 5 minutes at room temperature with Calcein-AM (C3099, Life Technologies) and subsequently sorted on a FACS Aria (BD ...
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bioRxiv - Developmental Biology 2023Quote: Cells were thawed according to the provider’s protocol and the medium was gradually changed to Essential 8 Flex Medium (E8f) (A2858501 Thermo Fisher / Life Technologies) supplemented with 100 μg/ml Normocin (4069-ant-nr-1 ...
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bioRxiv - Bioengineering 2023Quote: ... Washed cells were resuspended in 150 μL of FACS buffer and treated with anti-mouse MHC class I antibody conjugated to PE (Clone:28-14-8) (Invitrogen, USA) at a concentration of 2μg per sample and anti-mouse MHC class II antibody conjugated to FITC (Clone ...
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bioRxiv - Immunology 2023Quote: Purified CD11c+ myeloid cells (5 × 104 cells) from bone marrow cultures were plated on an 8 well glass chamber slide (Nunc Lab-Tek-Thermo Fisher) in supplemented IMDM medium and incubated for 24 h ...
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bioRxiv - Biochemistry 2023Quote: ... Freshly isolated mitochondria were resuspended in 50 μl of click-it lysis buffer (50 mM TRIS-HCl pH 8, 1% SDS, 250 U/mL Universal Nuclease [Thermo Fisher]) and incubated on ice for 15 min ...
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bioRxiv - Biophysics 2023Quote: ... 8-μm-thick cryosections were then cut with a Microm Cryo Star HM 560 cryostat (Thermo Fisher Scientific, Waltham, MA, USA) and mounted on microscope slides (Superfrost UltraPlus ...
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bioRxiv - Microbiology 2023Quote: Human ileocecal adenocarcinoma cells (HCT-8; ATCC CCL-244) were cultured in RPMI 1640 ATCC Modification medium (Thermo Fisher A14091-01) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were cultured to confluence and passaged 4 times before plating to 8-chamber slides (Thermo Fisher Scientific Cat#177445) coated with Matrigel (Thermo Fisher Scientific Cat# CB-40230 or Corning Cat# 356230 ...
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bioRxiv - Neuroscience 2023Quote: Adult DRG from adult C57BL/6J mice (8-10 weeks old) were rapidly dissected and placed in ice-cold DMEM/F12 (Gibco 11330057). DRG were washed 3 times with ice-cold calcium- and magnesium-free HBSS (Gibco 14175095 ...
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bioRxiv - Biophysics 2023Quote: ... Lysates were clarified by centrifugation at 13,000 rpm for 20 min in a Sorvall RC6+ centrifuge using a F21S-8×50y rotor (Thermo Fisher Scientific) at 37°C and incubated with 0.5 mL of Ni–NTA beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded into 6-well plates 24 h prior to transfection, 50 nM siRNA and 8 μL INTERFERin (Polyplus, France) were mixed in Opti-MEM (Gibco, USA), incubated for 10 min at room temperature and added to the cells ...
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bioRxiv - Molecular Biology 2023Quote: ... feeder-free mouse ESCs were grown as aggregate culture for eight days (DIV-8) in differentiation media consisting of Glasgow’s MEM (GMEM) (Thermo Fisher Scientific) supplemented with 5% Knockout Serum Replacement (KSR ...
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bioRxiv - Cancer Biology 2023Quote: Untreated MCF-7 and MDA-MB-231 cells were seeded at 2 x 104 cell per well on 8-well glass chamber slides (™ Nunc™ Lab-Tek™ II Chamber Slide™ ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were resuspended in 2 mL of 8 M urea (containing 50 mM HEPES, pH 8.5) and protein concentrations were measured by BCA assay (Thermo Fisher Scientific) before protease digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS and subsequently incubated for 2 hours with fresh culture media containing 10 μL of CCK-8 solution (catalog no: NC9261855, Fisher Scientific) added in each well ...
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bioRxiv - Cancer Biology 2023Quote: Clear lysates from cells and EVs in 8M urea buffer (8 M urea, 75 mM NaCl, 50 mM Tris) were quantified using microBCA Protein Assay Reagent Kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and cultured at 37°C in a humidified 8% CO2 incubator with constant rotation at 130 RPM using Expi293 Expression Medium (ThermoFisher Scientific). DNA transfections were conducted using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by sodium dodecyl sulfate/polyacrylamide (SDS/PAGE) gel electrophoresis on 1 mm NuPAGE 3 – 8% gradient Tris-Acetate gels (Thermo Fisher) using Tris-Acetate SDS running buffer and transferred to nitrocellulose membranes (Bio-Rad ...