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Citations for Thermo Fisher :
2451 - 2500 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: Viability was assessed after 8 weeks of culture by staining with 4 μM ethidium homodimer-1 and 2 μM calcein AM (Thermo Fisher Scientific). Any condition where at least one viable cell was identified was considered positive for the presence of viable cells ...
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bioRxiv - Cancer Biology 2019Quote: ... The membranes were then washed with TBS-T buffer followed by exposure to the appropriate horseradish peroxidase-conjugated secondary antibody for 1h and visualized on either Kodak X-ray film (GeneMate, F-9023-8×10) or BioRad ChemiDoc Imaging System using either SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, 34080) or ECL Prime Western Blotting Detection Reagent (Amersham ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... RPE-choroid explants were washed with phosphate-buffered saline (PBS) and 300 μl of 8 µg/ml Calcein AM (Thermo Fisher Scientific) applied to each explant and incubated for 1 h at 37°C (5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: Passaging in competition assays was performed by co-transfecting BHK-21 cells with in vitro transcribed replicon RNA and harvesting total cell RNA at 8 hours post transfection using TRIzol reagent (Thermo Fisher Scientific). The harvested RNA was then purified using the Direct-zol RNA MiniPrep kit (Zymo Research ...
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bioRxiv - Pathology 2019Quote: PMLECs between the 3rd and 5th passages were seeded (8 × 104/well) in 2-well Permanox chamber slides from Lab-tek (Thermo Scientific, Nunc). The cells were incubated at 37°C and 5% CO2 for 24 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... 11-7139-42 or 85BRD, cat.: 11-7136-42), IL-4-PE (8D4-8, cat.: 12-7049-42) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11 ...
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bioRxiv - Cancer Biology 2019Quote: ... The spheroids were cultured for 8 days and pictures were taken with an AMG Evos microscope 40x objective (Thermo Fisher Scientific Inc). Spheroid areas were measured using ImageJ 1.51j8 software (National Institute of Health ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from the H661 cells after DMSO or ENT treatment (duplicate samples for each treatment) for 8 hours using TRIzol (Invitrogen, Carlsbad, USA) and a miRNeasy mini kit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... Following tissue digestion 8 ml of resuspension media (DMEM/F12 with glutamax supplemented with 15%FBS and 1% pen/strep; Thermo Scientific, CA) was added to stop enzyme activity ...
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bioRxiv - Microbiology 2021Quote: ... and 2×105 cells were seeded into 24-well culture plates or 5×104 cells into Lab-Tek II 8-well chamber slides (Nunc/Thermo Fisher). To account for donor variations ...
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bioRxiv - Biophysics 2020Quote: ... were plasma cleaned for 10 min and attached with the use of dental imprint silicon putty (Picodent twinsil 22, Picodent) to Lab-Tek™ 8-well chambers (ThermoFisher Scientific), from which the glass bottom had been removed42 ...
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bioRxiv - Biochemistry 2020Quote: ... 6 to 8-week old male mice (C57BL/6J) were injected via tail vein with 29 G syringe (ThermoFisher 14-841-32) at a dose of 10e11 GC/mouse diluted in saline in a total volume of 100 μl/mouse ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were dissociated by papain digestion and plated on poly-D-lysine-coated (0.01 mg/mL) 8-well Lab-Tek II chambered cover glass (Thermo Fisher Scientific, #Z734853). Specifically ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg protein was loaded per well on 8% acrylamide gels with prestained standard molecular weight markers (PageRuler prestained protein ladder; Thermo Fisher Scientific) and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... Overexpressed myc-flag-tagged cadherin-8 was immunoprecipitated using the Pierce c-My-tag IP/Co-IP kit according to the manufacturer’s protocol (Thermo Fisher Cat#23620). Co-immunoprecipitated proteins were determined by Western blot analysis.
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bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... 20 μl of untreated and of ProK treated PMV (before dilution for FRET) was loaded to 8 % SDS-PAGE gels and the proteins were stained with GelCold Blue Stain (Thermo Fisher Scientific). A control in which PMSF was added for 15 min at room temperature before adding ProK also prepared and loaded on the gel.
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bioRxiv - Physiology 2021Quote: ... aliquots of 30µg of protein were prepared in native Tris glycine sample buffer and analysed from NuPAGE 3-8% Tris-Acetate polyacrylamide gel electrophoresis in a tris-glycine native running buffer (Thermo Fisher Scientific). After 2-hours of migration at 130 V ...
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bioRxiv - Neuroscience 2021Quote: ... Human iPS cells were maintained and propagated as colonies in feeder-free conditions in Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES qualified (Corning ...
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bioRxiv - Microbiology 2021Quote: ... and sacculi were spun down at 22,000 x g at 40 °C for 30 min in a Heraeus Multifuge X1R using a Fiberlite™ F15-8 x 50cy Fixed Angle Rotor from Thermo Scientific. SDS was removed by washing the sacculi with 60 °C pre-warmed Millipore water several times until no SDS was detected by the methylene blue assay [Hayashi ...
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bioRxiv - Neuroscience 2020Quote: ... The middle segment was used to cut 8 μm serial sections using rotary microtomes (HM 200 and HM 355; Thermo Scientific, Germany) which were mounted on glass slides ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 8% of fetal bovine serum (FBS) and 1% of penicillin-streptomycin (PES 10, 000 u/mL, Thermo Fisher, NY, USA). Macrophages were seeded over sterile coverslips in a ratio of 250 × 103 / cm2 and they were maintained at 5% CO2 atmosphere at 37 °.
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bioRxiv - Microbiology 2020Quote: ... U-2 OS cells were cultured to 80% confluency in Nunc™ 8-well Lab-Tek II Chambered Coverglass slides (Thermo Scientific). Upon infection ...
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bioRxiv - Microbiology 2020Quote: ... EBV producer HEK-293 cells stably transformed by BART-repaired B95-8 based EBV BAC system encoding GFP (45) were cultured in RPMI 1640 (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... maintenance culture was carried out for all cell lines including human ES cells using albumin-free Essential 8 Medium (Thermo Fisher Scientific) in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N ...
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bioRxiv - Microbiology 2020Quote: ... Cover slips were then washed three times for 5 min in wash buffer and subsequently mounted on slides with 8 μl of prolong glass (Invitrogen, cat. # P36984) or mowiol ...
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bioRxiv - Cancer Biology 2020Quote: ... A431RON (Figure 3A-B and 4) or A431RON-K1114M (Figure S3B) cells were seeded in 8-well chamber slides (Nunc Lab-Tek) at a density of 30,000/well and allowed to adhere overnight ...
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bioRxiv - Cell Biology 2021Quote: ... An aliquot of lysate was taken and diluted 1:5 in 8 M Urea from which protein concentration was determined by BCA assay (Thermo Fisher Scientific). Aliquots corresponding to 100 μg of protein were subsequently diluted in SDC buffer into a 96-well deep-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
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bioRxiv - Cancer Biology 2020Quote: ... After 24 h, CCK-8 (Dojindo, Japan) was used to measure drug sensitivity at 450 nm using a microplate reader (Thermo Fisher, USA) after incubating at 37 °C for 1–2 h.
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bioRxiv - Cancer Biology 2021Quote: ... colonoids were dissociated using TrypLE and replated in an 8-well glass-bottom chamber slide (Nunc™ Lab-Tek™, Cat#155411). Three to four days later ...
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bioRxiv - Cell Biology 2021Quote: ... On-feeder hiPSC cultures were gradually transitioned to feeder- free conditions by weaning off from the standard HuES media to Essential 8™ medium (Gibco, #A1517001) and maintained on hESC-qualified Matrigel coated surface ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 mM ATP in kinase buffer (20 mM Tris pH 8, 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 1X Phosphatase inhibitor (Thermo Scientific, #1862495)) at room temperature for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
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bioRxiv - Cell Biology 2022Quote: ... Human iPSC lines were cultured feeder-free on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix and maintained in Essential 8 Flex Basal Medium (Thermo Fisher Scientific) supplemented with Essential 8 Flex Supplement (50x ...
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bioRxiv - Molecular Biology 2022Quote: ... The retinas were washed in PBS for 30 min at least 8 times at room temperature and incubated with anti-IB4 (I21412, Invitrogen, 1:200) and Alexa Fluor 488 goat anti-rabbit IgG (A11008 ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were killed with 10 μL of 6X SDS loading buffer and pooled or directly loaded onto a 15-well Novex Wedgewell 8% Tris-Glycine Gel (Thermo Fisher Scientific) and ran at 175 V for ~ 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of sample (which is the equivalent of 10 embryos) was loaded into each lane of a 3-8% Tris-Acetate pre-cast SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific) and afterwards transferred from the gel onto a nitrocellulose membrane (BIO-RAD ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: DRG cultures for immunocytochemistry and RNAscope experiments were prepared as described above with the neurons plated on 8-well Chamber Slides (154534; Nunc Lab-Tek). The neurons were resuspended in culture media ...
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bioRxiv - Neuroscience 2019Quote: Wild-type C57/BL6J mice of either gender (aged 8-10 weeks) were used as recipients of yellow-green microsphere (polystyrene FluoSpheres™, 505ex/515em nm, Invitrogen, USA) transplantation ...
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bioRxiv - Cell Biology 2021Quote: ... enzyme activity was stopped with 8 ml of resuspension media (DMEM/F12 with glutamax supplemented with 15%FBS and 1% pen/strep; Thermo Scientific, CA). The digestion mixture was passed through 100 μm cell strainer and centrifuged at 150 x g for 8 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Denatured samples from both the total lysates and surface fractions were separated on NuPAGE 3-8 % Tris-Acetate protein gels (Thermo Fisher Scientific) at 200 V for 40 min ...
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bioRxiv - Molecular Biology 2020Quote: The human embryonic stem cell (hESC) line Hues9 (H9) was obtained from the Wicell Research Institute (Madison, WI) and maintained in Essential 8 media (Thermo Fisher Scientific) on hESC-Qualified Matrigel (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... protein samples were boiled at 95°C for 5 min before separating on Novex WedgeWell 8–16% Tris-Glycine Mini Gels (Thermo Fisher Scientific). Protein samples were transferred to polyvinylidene fluoride membrane (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... all sections were autoclaved for antigen retrieval and immunohistochemical stained with anti-phosphorylated antibodies AT-8 (pSer202/pThr205, MN1020, Invitrogen, 1:250). Immunolabeling was then examined using a DM4000 microscope (Leica ...