Labshake search
Citations for Thermo Fisher :
2401 - 2450 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Pellets were resuspended in 2 mL of 8 M urea (containing 50 mM HEPES, pH 8.5) and protein concentrations were measured by BCA assay (Thermo Fisher Scientific) before protease digestion ...
-
bioRxiv - Cell Biology 2023Quote: HeLa wild-type or HeLa POLE4 KO cells were grown in 8-well Lab-Tek II chambered coverglass 30 (Thermo Scientific) and transfected 48 h prior to imaging with GFP-tagged PARP1 chromobody (Chromotek) ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Binding of compound to plasma proteins was determined by equilibrium dialysis of 3 µmol/L compound in plasma or serum against PBS through an 8 kDa molecular-weight cut-off cellulose membrane (RED device, Thermo Fisher) at 37 °C for 5 h ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
-
bioRxiv - Physiology 2024Quote: Total mRNA was extracted from 5 biological replicates of 8 females (once-mated, aged 9 d) for each genotype using Trizol reagent (Invitrogen 15596018) and Direct-zol RNA Miniprep Plus Kits (R2072) ...
-
bioRxiv - Immunology 2024Quote: ... Jurkat cells were added to the imaging buffer in the antibody(ies) and/or ligand coated 8-well coverslip chambers (Lab-Tek, Thermo Fisher) and imaged at room temperature (21 °C) ...
-
bioRxiv - Neuroscience 2024Quote: ... we counted the number of TH+/PHOX2B+ and TH+/PHOX2B- cells along the ventrolateral medulla (8 sections/rat; 240 µm interval) using an EVOS fluorescent microscope (ThermoFisher Scientific).
-
bioRxiv - Biophysics 2024Quote: ... against PIEZO1 that targets the 5’-AGCATTGAAGCGTAACAGGG-PAM-3’ at Chr.8: 122513787 - 122513809 on GRCm38 was purchased from Invitrogen (A35533). The cells were transfected with the Cas9 enzyme (TrueCut Cas9 Protein v2 ...
-
bioRxiv - Biophysics 2024Quote: ... 100 mM NaCl and centrifuged again at 40,000 x g for 20 min at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). Inclusion bodies were then solubilized in 50 mM Tris pH 8.0 ...
-
bioRxiv - Cell Biology 2024Quote: ... ∼11,000 cells of either WT or CCSer2-KO cells were plated per well of an 8-well live imaging dish (Thermo Fisher) coated with fibronectin ...
-
bioRxiv - Cell Biology 2024Quote: ... ∼11,000 cells of either WT or CCSer2 KO cells were plated per well of an 8-well live imaging dish (Thermo Fisher) coated with fibronectin ...
-
bioRxiv - Bioengineering 2024Quote: ... 100 μL of cell-containing hydrogels were added to the bottom of a well in 8-well LabTek chamber (#155360, Thermo Scientific). After 30 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Click-it reaction was performed after staining with primary antibodies by incubating cells in Click-it buffer (100 mM Tris-HCl pH 8, 2 mM CuSO4, 1 ng Alexa Fluor 647 Azide (Life Technologies), and 100 mM ascorbic acid for 30 min at room temperature (RT) ...
-
bioRxiv - Biophysics 2024Quote: ... 50 mM DTT for 30 min at room temperature and non-solubilized material was removed via centrifugation at 40,000 x g for 20 min at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific).
-
bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 40,000 x g for 60 min at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). The cleared lysate was then loaded onto Ni-NTA (QIAGEN ...
-
bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 40,000 x g for 30 min at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). The pellet was resuspended in 20 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 87,207 x g for 1 hr at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). All subsequent purification steps were carried out in a cold room at 4 °C in analogy to SKP1-FBXO22.
-
bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 87,207 x g for 1 hr at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). All subsequent purification steps were carried out in a cold room at 4 °C ...
-
bioRxiv - Biophysics 2024Quote: ... The lysate was subsequently cleared by centrifugation at 87,207 x g for 2 hrs at 4 °C using a Sorvall LYNX 6000 centrifuge and a A27-8 x 50 fixed angle rotor (both Thermo Scientific). The clear lysate was loaded onto a 5 mL StrepTrapHP column using an ÄKTA pure chromatography system (Cytiva) ...
-
bioRxiv - Immunology 2024Quote: CD8+ T cells were immobilized in 8-well poly L-lysine-coated IBIDI chambers and stimulated by the addition of CD3/28 Dynabeads (cat. 11452D, Thermo Fisher) for 30 min ...
-
bioRxiv - Biochemistry 2024Quote: ... 300 mM NaCl pH 8) and purified by nickel-charged IMAC using a 96-well HisPurTM Ni-NTA Spin Plate (ThermoFisher Scientific) equilibrated in Equilibration Buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... the media from the well of a LabTek II Chambered Coverglass with Cover #1.5 Borosilicate Sterile 8-well plate (Thermo Fisher 155409) was removed and replaced with 200 μL of complete media ...
-
bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of TMT-labeled peptides were pooled and fractionated into 8 fractions using a High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... The co-culture was transfected with the 8-plasmid set (2 μg of each plasmid) using Lipofectamine 2000 transfection reagent (Fisher Scientific). At 24 h post transfection ...
-
bioRxiv - Immunology 2024Quote: Jurkat T cells were stimulated by addition in imaging buffer to the antibody/ies and/or ligand-coated 8-well coverslip chambers (Lab-Tek, Thermo Fisher). Incubation was for 5 minutes at room temperature (21 °C) ...
-
bioRxiv - Cell Biology 2024Quote: ... The mobile phase was prepared from 8 μl of a solution of nanoparticles cross-linked with antibodies to BSA (ThermoFisher, A11133), 4 μl of casein solution (0.2 mg/ml ...
-
bioRxiv - Developmental Biology 2024Quote: ... incubated for 30 minutes in a live/dead Viability/Cytotoxicity solution containing 4 mM Calcein and 8 mM Ethidium homodimer-1 (L3224, ThermoFisher Scientific) at 37 °C ...
-
bioRxiv - Developmental Biology 2024Quote: ... NMP-L cells were replated at end of D3 and cultured for 8 to 9 days and transfected using Lipofectamine 3000 kit (Thermo Fisher) following manufacturer’s instructions with a combination of piggyBac plasmids (pm-eGFP + HypBase or pm-mKate +H2B-mEos3.2 + HypBase ...
-
bioRxiv - Molecular Biology 2024Quote: Western blotting was performed by standard procedures using Novex Tris-Glycine buffered gradient WedgeWell gels (8-16%) (Invitrogen – Thermo Fisher Scientific) in a Mini Cell apparatus ...
-
bioRxiv - Molecular Biology 2024Quote: Western blotting was performed by standard procedures using Novex Tris-Glycine buffered gradient WedgeWell gels (8-16%) (Invitrogen – Thermo Fisher Scientific) in a Mini Cell apparatus ...
-
bioRxiv - Cell Biology 2024Quote: Flp-In T-REx HeLa cells of the genotypes indicated in the figure legends were plated in 8-well Lab Tek II Chamber 1.5 German coverglass dishes (Thermo Fisher 155409) in regular growth medium ...
-
bioRxiv - Microbiology 2024Quote: ... between 5 and 50 μL of cell sample was dropped onto either poly-l-lysine coated chambered slides (Nunc Lab-Tek 8-Well chambered slides, Thermo scientific) or 10-well diagnostic slides (MP Biosciences ...
-
bioRxiv - Neuroscience 2024Quote: ... coronal sections (8 µm) were taken at the level of the VTA and immediately mounted onto Superfrost Plus slides (Fisher Scientific). Glp1r ...
-
bioRxiv - Genomics 2024Quote: ... DNA samples were eluted in 30 µl 10 mM Tris HCl pH 8 and analysed for purity and quantity using the Nanodrop spectrophotometer (NanoDrop One, ThermoFisher Scientific) and Qubit fluorometer (Invitrogen ...
-
bioRxiv - Biophysics 2021Quote: ... were plasma cleaned for 10 min and attached with the use of dental imprint silicon putty (Picodent twinsil 22, Picodent) to Lab-Tek™ 8-well chambers (ThermoFisher Scientific), from which the glass bottom had been removed34 ...
-
bioRxiv - Cancer Biology 2021Quote: MEFs were plated in 8-well cover glass bottom chambers (Lab-tek #155409) and loaded with cSNARF-1-AM [10 μM] (Thermo Fisher #C1272), a fluorescent pH-sensitive dye ...
-
bioRxiv - Cancer Biology 2021Quote: ... each coverslip was inverted onto a 30- μl droplet of 1:8 Oregon Green™ 488 Conjugate Gelatin (Life Technologies, Cat. #G13186) with 0.1% porcine gelatin (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... tissue culture flasks or MATTEK glass bottom dishes were incubated in sufficient volume of a mixture of Essential 8 (E8) media (ThermoFisher cat. # A1517001) and Matrigel (Corning cat ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5-8 × 106 autogamous cells (T=50 hours) were collected by centrifugation and cross-linked with 1% methanol-free formaldehyde (Thermo Fisher Scientific) PHEM 1 × (10 mM EGTA ...
-
bioRxiv - Neuroscience 2021Quote: ... a transcription factor neurogenin-2 in a doxycycline inducible cassette was stably integrated into the AAVS1 safe harbour locus of iPSCs cultured in Essential 8 media (ThermoFisher Scientific, A1517001). The expression of neurogenin-2 in the iPSCs was induced for three days using doxycycline (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 5 μL of input and 10 μL of eluate were separated on NuPAGE 3-8 % Tris-Acetate gels (Thermo Fisher Scientific) run at 4 °C and 35 mA/gel for 1.5 h ...
-
bioRxiv - Cell Biology 2022Quote: ... about 5×104 of transfected cells were digested and placed into the upper chamber precoated 8 μm pore transwell insert (Fisher Scientific, 0877121) with the lower chamber containing the medium (containing 10% FBS) ...
-
bioRxiv - Cell Biology 2022Quote: ... frozen WTC11 cells were first thawed in 37 °C water bath and washed in Essential 8 Medium (E8; Thermo Fisher Scientific, #A1517001) by centrifugation ...
-
bioRxiv - Developmental Biology 2022Quote: ... The bottom layer of the culture was first prepared by pouring 100 μL of type I collagen mix solution into each well of an 8-well coverglass chamber (177402PK, Nunc, Roskilde, Denmark). The middle layer of the culture was prepared by adding 100 μL of the type I collagen mix solution containing 3 × 103 cells/well of the Klf5-iKO line and laid over the bottom layer ...
-
bioRxiv - Genomics 2020Quote: ... A subsample of approximately 25 larvae was collected from each replicate jar on days 4 and 8 and stored in RNAlater (Thermo Fisher Scientific) for gene expression profiling.
-
bioRxiv - Immunology 2021Quote: ... The peptides from the Hemocyte samples collected at four different time points were labelled with four 8-plex iTRAQ reagents (Applied Biosystems, USA).
-
bioRxiv - Pathology 2019Quote: ... with 8 mM EDTA for 10 min at 75 °C and then converted to cDNA using Superscript IV reverse transcriptase (Invitrogen, Stockholm, Sweden) in a 20 μl reaction ...