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Citations for Thermo Fisher :
2251 - 2300 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... mixed at an 1:1 ratio in a test tube and added to wells of an 8-well slide (Thermo Fisher) coated with poly-lysine (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Solubilized membranes were mixed 1:1 with binding buffer (20 mM Tris pH 8/1 M NaCl/20 mM imidazole) and loaded onto a HisPur Ni-NTA (Thermo Scientific) column equilibrated with 20 mM Tris pH 8/750 mM sucrose/500 mM NaCl/10 mM imidazole/0.2% DDM (equilibration buffer) ...
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bioRxiv - Cancer Biology 2020Quote: After 48 hours incubation in the presence of the compounds, cells were fixed by the addition of an equal volume of formaldehyde (8 %, 50 µL) (#28908, Thermo Scientific) to the existing media ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were analyzed by using an 8 capillary ABI 3500 DNA Genetic Analyzer with POP-4 polymer (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... ermGal4) or their unsorted control matches were plated on cover glass (Labtek II Chambered Coverglass, 8-well, 155409, Thermo Fisher Scientific) into Schneider’s medium (Homem ...
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bioRxiv - Systems Biology 2019Quote: ... Equivalent amounts of protein in the specimen were separated based on their molecular weight by electrophoresis on 8% Bis-Tris gels and transferred to polyvinylidene difluoride (PVDF) membranes (Life Technologies). Membranes were immediately blocked with 5% nonfat dried milk diluted in TBST and incubated with primary antibodies (1:1,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then denatured at 95°C for 8 min in the presence of 4X LDS sample loading buffer (Life Technologies) and 5% beta-mercaptoethanol (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: Dried fractions were resuspended in 8 μL 5% ACN + 5% FA and analyzed on an Orbitrap Fusion with in-line Easy Nano-LC 1000 (Thermo Scientific). Fractions were run as 3 h gradients progressing from 3% ACN + 0.125% FA to 100% ACN + 0.125% FA ...
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bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
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bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
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bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...
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bioRxiv - Cell Biology 2021Quote: ... generated from reprogrammed dermal fibroblasts by retrovirus carrying the Yamanaka gene factors) was maintain on Matrigel-coated culture dishes in Essential 8 Flex Medium (ThermoFisher Scientific). The protocols for culturing cell lines and differentiating the hiPSCs into HBs ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were passed through a 25G syringe five times to reduce viscosity and separated on a 3%-8% NuPAGE Tris-Acetate gel (Thermo Fisher). Proteins were transferred onto a polyvinylidene fluoride membrane (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... High-resolution mass spectra and MS/MS fragmentation pattern of 1-8 were recorded using a Q Exactive Plus mass spectrometer (Thermo Scientific). Chromatography for determination of amino acyl hydroxamate was performed on a Waters ACQUITY H-class UPLC system coupled to a Xevo TQ-S micro (Waters ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were seeded on poly-L-lysine-coated (MW > 300,000, 0.1 mg/ml, Biochrom) 8-well chamber slides (Nunc Lab-Tek II) and transfected the next day (50-70% confluency) ...
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bioRxiv - Biochemistry 2021Quote: ... then lysed in Triton lysis buffer (1% Triton, 150 mM NaCl, 50 mM Tris pH 8, 1× Halt protease-inhibitor cocktail (Thermo Scientific)) ...
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bioRxiv - Cancer Biology 2021Quote: ... The fluorescent reaction was measured every 40 sec for 8 min and subsequent every 5 min for 6 times using a microplate reader (Thermo Scientific) with enzyme kinetics model ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Immunology 2020Quote: ... 35 and 42 dpv by venepuncture of the external jugular vein: 8 mL/pig in BD SST vacutainer tubes (Fisher Scientific) for serum collection and 40 mL/pig in BD heparin vacutainer tubes (Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... ΔA ΔfabR ΔserB was generated at the OSU Unnatural Protein Facility as previously described.8 DH10b was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then analysed by SDS-PAGE on 8% polyacrylamide gels using a PageRuler™ Prestained Protein Ladder (Thermo Scientific, 26616). Proteins where then transferred onto a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells were transiently transfected with the indicated siRNA at a final concentration of 8 nM using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Neuroscience 2020Quote: ... heated at 95 °C for 5 min and were run in on 8–16% NuPAGE SDS-PAGE gradient gel (Life Technologies) in MOPS SDS Running Buffer (Life Technologies ...
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bioRxiv - Immunology 2020Quote: Sh3gl1-/- and WT littermates were immunized at 8-10 weeks of age by intraperitoneal injection of 50 μg NP-CGG in alum (Thermo Fisher). Blood samples were taken before immunization and at 7 and 14 days after immunization ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted RNA from sets of 8 adult males (2~4 day old) in triplicate from each RNAi cross using TRIzol (Catalog# 15596-026, Invitrogen, USA), treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs ...
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bioRxiv - Neuroscience 2021Quote: All iPSC lines were maintained on vitronectin-coated plates and fed with Essential 8 (E8) + E8 supplement media (ThermoFisher, CAT#: A1517001).
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bioRxiv - Microbiology 2019Quote: ... Crosslinking was performed by incubating purified pUL7:pUL51(8–142) with DSSO or DSBU and mass spectra were acquired on an Orbitrap Fusion Lumos (Thermo Fisher). pUL7:pUL51(8–142 ...
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bioRxiv - Immunology 2021Quote: ... 2.5 x105 CFSE-labeled CD8+ T cells were cultured with J774M cells at varying ratios (1:1 – 1:8 J774M:T cells) and anti-CD3/CD28 beads (ThermoFisher, cat. 11453D) at a bead-to-T cell ratio of 1:1 per the manufacturer’s instructions for T cell activation ...
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bioRxiv - Immunology 2020Quote: 7575 naïve CD4 cells were isolated from lymph nodes of CCT8T+/+ and CCT8T−/− mice at 4-8 weeks of age by FACS sorting directly into lysis buffer (RIPA buffer (ThermoFisher Scientific) + 4 % IGEPAL CA-360 (Merck) ...
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bioRxiv - Physiology 2020Quote: ... Samples were then subjected to offline reversed phase fractionation (8 fractions) using a Pierce High-pH Reversed Phase Fractionation Kit (Thermo Fisher) according to the manufacturer’s instructions for fractionation of TMT-labeled samples.
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bioRxiv - Immunology 2020Quote: ... 42 and 56 days post-immunisation (DPI) by venepuncture of the external jugular vein: 8 mL/pig in BD SST vacutainer tubes (Fisher Scientific) for serum collection and 40 mL/pig in BD heparin vacutainer tubes (Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Developmental Biology 2021Quote: ... FAPs were plated at a starting density of 3 X 104 cells/cm2 and grown for 8 days in DMEM (Life Technologies) containing 5% FBS plus 1% Pen/Strep ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: PSCs grown in Essential 8 Flex medium (see Supplementary material for details on PSC generation, characterization and maintenance) were dissociated by TrypLE (Life Technologies) to single cells and seeded on human recombinant laminin 521 (BioLamina)-coated plates in Essential 8 Flex medium at a density of 7 x 104 cells/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cut in 16 μm coronal sections into 8 series that were mounted directly on slides (Superfrost Plus; Fisher Scientific) and stored at −45°C until receptor autoradiography was performed ...
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bioRxiv - Genomics 2021Quote: ... Then 100 μ L of this solution was gently mixed with 8 μ L of Lipofectamine™ 2000 (lipo) (Invitrogen, USA). A solution of lipo and siRNA (total volume 200 μ L ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Cancer Biology 2020Quote: ... The second half of the data (runs 5-8) was obtained after peptide separation on 75 μm columns packed with 2.6 μm Accucore beads (Thermo Fisher Scientific). Peptides were injected onto 30-40 cm ...
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bioRxiv - Immunology 2022Quote: ... Cross-linking occured for 30 min in a rolling tube after which beads were washed with 0.2 M ethanol amine pH 8 (#149582500, Thermo Fisher Scientific) to quench the crosslinking reaction.
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bioRxiv - Neuroscience 2022Quote: Human induced pluripotent stem cells (hiPSCs) were maintained in feeder-free monolayers on Geltrex (ThermoFisherScientific) and fed daily with Essential 8 medium (Life Technologies). When confluent ...
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bioRxiv - Neuroscience 2022Quote: ... They were cultured in 37C with 5% CO2 on geltrex-coated tissue culture plates in Essential 8 complete medium (E8; Gibco, #A1517001) with daily media changes ...
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bioRxiv - Cell Biology 2022Quote: ... and 4.8 µL cDNA (diluted 1:20 prior to use) was added to each well of qPCR plates (REF 4346906, Life Technologies). Samples were run on a StepOnePlus™ RT-qPCR machine (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... cells were maintained at a density of 2-8×105 cells/ml in Roswell Park Memorial Institute (RPMI) 1640 Medium (GIBCO, USA) and supplemented with 10% horse serum (GIBCO ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...