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Citations for Thermo Fisher :
2551 - 2600 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and 25ug of cell lysates from each condition were resolved by 8% SDS-PAGE with NuPAGE electrophoresis system (Invitrogen/Thermo Fisher Scientific). Proteins were then transferred to PVDF membrane (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... diluted into to 500 μl of 107 cfu/ml in the wells of an 8-well Nunc Lab-Tek II chamber cover glass (Thermo Fisher Scientific). The cover glass was covered with the provided cap and incubated at 28°C for 72 h ...
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bioRxiv - Microbiology 2022Quote: ... HeLa cells and cortical neurons were cultured on Nunc Lab-Tek II 4 or 8-well glass chamber slides (Thermo Fisher Scientific). After exposure to autophagy inducing conditions ...
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bioRxiv - Microbiology 2022Quote: ... Lung organoids were maintained for experiments between passage 2-8 prior to dissociation with TrypLE and seeding onto Geltrex (Gibco, Cat. A1413201) -coated plates supplemented with Y-27632 (Selleck Chemicals ...
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bioRxiv - Microbiology 2022Quote: Mammalian cells were seeded at a density of 8×105 in a 96-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Plant Biology 2022Quote: ... and surface sterilized with undiluted household bleach (Clorox) at 8% sodium hypochlorite and sown on 1% water agarose (Life Technologies Catalog: 16500100) plates.
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bioRxiv - Neuroscience 2022Quote: ... The cells were grown in an atmosphere of 37°C and 8% CO2 and were passaged following trypsinization with Trypsin-LE (Gibco, Waltham, MA).
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bioRxiv - Microbiology 2024Quote: ... Single-axis tilt series were collected covering an angular range from -65° to +65° or -60° to +60° at 6- to 8-μm underfocus using the Tomography software package (Thermo Fisher Scientific). Subtomogram averaging was done the eman 2.99 software package ...
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bioRxiv - Cell Biology 2024Quote: 3T3-L1 Mouse embryonic fibroblasts (MEFs)(ATCC, State, USA)) cells were cultured in 8-well Nunc Lab- Tek® II glass chamber slides (Fisher Scientific) in standard cell culture conditions (CO2 5% ...
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bioRxiv - Cancer Biology 2024Quote: ... The resolved samples were run on either NuPAGE 3–8% Tris-acetate or NuPAGE 12% Bis-Tris protein gels (Thermo Fisher Scientific) with the appropriate running buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and 40 μg of protein lysates were resolved on precast 3-8% gels in Tris-acetate buffer and then transferred to nitrocellulose membranes (all from Thermo Fisher Scientific). Membranes were blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: U2OS or ES-2 cells expressing either the EGFP-fused WWE domains or EGFP-fused Af1521 macrodomain variants were cultured in wells of an 8-chamber glass bottom vessel (Thermo Fisher Scientific) at a density of 7x104 cells per well ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) in 8% polyacrylamide gel together with PageRuler protein ladder (Thermo Fisher Scientific) and transferred onto polyvinylidene difluoride membrane (PVDF) ...
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bioRxiv - Neuroscience 2024Quote: ... A CFSE working solution was prepared by adding 8 µL of DMSO and 1 µL of Fast Green to one vial of CellTrace CFSE (CellTraceTM CFSE, Life Technologies, #C34554) for a final concentration of 10 mM ...
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bioRxiv - Microbiology 2024Quote: ... The grids were blotted for 14 s at a humidity of 100% at 8 °C and rapidly frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Cryo-EM samples were initially screened using a Glacios cryo-EM operated at 200 kV and equipped with a Falcon4 camera installed at the Institute for Life and Medical Sciences ...
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bioRxiv - Genetics 2024Quote: ... gel at 75 V for 8 h at 4°C after which gels were stained for 24 h with SYBR GOLD (Invitrogen cat. S11494) diluted 1:10000 in 1X Tris-glycine (Bio-Rad cat no ...
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bioRxiv - Bioengineering 2024Quote: ... Flow cytometry to assess CAR and TCR positivity was performed on Day 8 of manufacturing on an Attune NxT flow cytometer (ThermoFisher, Waltham, MA). Immunophenotyping of cells was performed on Day 10 of manufacturing using a spectral immunophenotyping panel on an Aurora spectral cytometer (Cytek ...
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bioRxiv - Molecular Biology 2024Quote: ... 6-8-week-old WT mice were randomly selected to receive 2% dextran sodium sulfate (DSS) 40-50,000 MW (Affymetrix, Santa Clara, CA) in drinking water for 7 days followed by normal drinking water afterwards ...
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bioRxiv - Neuroscience 2024Quote: ... isolated primary nOPCs and aOPCs were plated in PDL-coated 8-well chamber slides and allowed to recover overnight (Thermo Fisher, 154739). The cells were incubated with 10 µM EdU for 5 h ...
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bioRxiv - Cell Biology 2024Quote: ... The border zone was roughly dissected and a pool of border zone cells from 8 hearts was dissociated using the Pierce Primary Cardiomyocyte Isolation kit (Thermo Fisher 88281) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Grids were blotted for 2 s in 100 % humidity at 8-12 ℃ and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected in counted mode in EER format on a CFEG-equipped Titan Krios G4 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Proteins were separated in denaturing conditions using Invitrogen Novex™ WedgeWell™ 8-16% gradient Tris–glycine acrylamide gels (Thermo Fisher Scientific) and transferred using a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Genetics 2024Quote: For each biological replicate a quarter of kidney tissue was used and dounced with a Wheaton Dounce tissue grinder in urea buffer (8 M Urea, 50 mM ammonium bicarbonate) supplemented with Halt protease-phosphatase-inhibitor cocktail (Thermo Scientific™). After clearing of the sample (16.000 xg ...
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bioRxiv - Genomics 2024Quote: Whole-cell extracts were prepared using RIPA buffer supplemented with protease inhibitors and proteins were separated by SDS-PAGE (8%) and blotted onto a nitrocellulose membrane using the dry transfer iBlot System (Thermo Fisher Scientific). Western blotting was performed using PBS-Tween 0.05% ...
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bioRxiv - Immunology 2024Quote: ... were cultured with 105 syngeneic BMDCs and stimulated with 100 live eggs plus 8 × 104 anti-CD3/CD28 coated beads (Gibco Dynal Dynabeads) for 72h.
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bioRxiv - Microbiology 2024Quote: ... and blotted for 1.5 s before being plunged into liquid ethane in 100% humidity at 8 °C using a Mark IV Vitrobot (Thermo Fisher Scientific). The datasets of Csy-DNA complex samples were collected using a Titan Krios microscope (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 14500xg for 12 minutes at 4°C in a Fiberlite F21-8 x 50y Fixed-Angle Rotor (Thermo Fisher, USA). The synaptosome-rich interface between 10% and 23% Percoll layers was collected and resuspended in 30 volumes of HB ...
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bioRxiv - Biochemistry 2024Quote: ... with a replacement in the N-terminal domain loop 8 (139CQKRDGPH146 to 139AEAG142, designated as rA3GR8) was constructed in the pET-sumo vector (Thermo Fisher Scientific). This construct generated a SUMO fusion with an N-terminal 6xHis tag and a PreScission protease cleavage site ...
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bioRxiv - Bioengineering 2024Quote: ... and 800 μL of a viral solution comprising concentrated lentiviral particles and 8 μg/mL polybrene was added to the cells (Thermo Fisher Scientific). Cells were then spinfected at 1048 × g for 90 min at 30 °C ...
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bioRxiv - Biochemistry 2024Quote: ... was incubated for 10 minutes at room temperature (∼22 °C) in reaction buffer containing the following components at their final concentrations: 25 mM Tris (pH 8; Thermo Fisher Scientific), 150 mM KCl (Sigma) ...
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bioRxiv - Biophysics 2023Quote: The cysteine-bearing Aβ40 variant was redissolved in 50 mM NaPi pH 7.5 and labelled with an 8-fold molar excess of Alexa Fluor™ 488 C5 Maleimide (AF488; Invitrogen Life Technologies) and incubated at room temperature for 2 h (Supplementary Figure 3) ...
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bioRxiv - Biophysics 2023Quote: ... and U-251 cells were seeded onto PLL-PEG-RGD-coated 8-well chambers in the respective medium with penicillin (1 unit/mL) and streptomycin (1 µg/mL; Gibco, Life Technologies) at densities between 0.5 x 104 to 2.5 x 104 cells/well ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from CD4 and CD8 T cells sorted from 8-week-old mice and was measured using the NanoDrop2000 (Thermo Fisher Scientific), agarose gel electrophoresis and Agilent2100 ...
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bioRxiv - Cell Biology 2023Quote: ... tracheas from adult (8- to 12-weeks old) mice were dissected and cleaned in ice-cold Ham’s F-12 medium (Life Technologies, #31765-092) with 1% penicillin/streptomycin (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved due to 3-8% Tris-acetate SDS-PAGE and transferred onto methanol-activated PVDF membrane with Pierce G2 Fast Blotter (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Microbiology 2023Quote: ... followed by flushing the solution with 20 μL of DPBS (KCl 0.2 g/L, KH2PO4 0.2 g/L, NaCl 8 g, Na2HPO4 1.15 g/L, pH = 7.0–7.3, Thermo Fisher Scientific, Waltham, MA, United States) (Figure S2A Step 3) ...
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bioRxiv - Biophysics 2023Quote: ... cells were passaged 1:8 and placed in selection media—DMEM growth media containing 1 ug/mL puromycin (Thermo Fisher #A11138-03)—and selected for 7 days ...
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bioRxiv - Biophysics 2023Quote: ... 150 μL of the diluted suspension was transferred into one well of a Nunc Lab-Tek II 8-well chambered coverslip (Thermo Scientific, USA) and the sample allowed to settle for 10 minutes to form a GUV monolayer at the bottom of the chamber ...
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bioRxiv - Biophysics 2023Quote: 150 μL of the washed vesicle suspension was transferred into one well of a Nunc Lab-Tek II 8-well chambered coverslip (Thermo Scientific, USA) and the sample allowed to settle for 10 minutes to form a GUV monolayer at the bottom of the chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were rocked for 2 min in the dark and incubated for an additional 8 min or longer (while protected from light) before reading luminescent output with a Varioskan LUX plate reader (Thermo Fisher, USA).
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bioRxiv - Microbiology 2023Quote: ... pH= 8) and 20uL proteinase K were applied to each culture and incubated at ambient temperature on a rotary shaker (Fisher Scientific, USA) for 45 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 20% Knockout Serum Replacement, 1% Glutamax, 1% Non-Essential Amino Acids, 0.1% ß-Mercaptoethanol, and 8 ng/mL bFGF, Thermo Fisher Scientific), and passaged weekly by manual disruption of 6-10 representative colonies ...
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bioRxiv - Cell Biology 2023Quote: ... these cell lines were maintained and propagated under feeder-free conditions using Essential 8 Medium on a geltrex-coated plate (ThermoFisher, Waltham, MA). The following iPSC lines were used in this study ...
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bioRxiv - Pathology 2023Quote: ... cells were plated uniformly at a density of 1 × 105cells/well in an 8-well chamber slide (ThermoFisher, 154453 or iBidi, 80826). Cells were washed after reaching confluence ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissected tails were then placed in individual wells of 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA) with 200µl of Gibco CO2 independent medium (Thermo Fisher #18045054).
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bioRxiv - Biochemistry 2023Quote: ... pombe SY78 cells were mixed in an 8:1 ratio for each condition and RNA was isolated using the Ribopure Yeast Kit (Ambion Cat# AM1924). 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066) ...
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bioRxiv - Bioengineering 2023Quote: CHO-suspension cells were plated at a concentration of 7.5x10^4 cells/mL in Freestyle CHO Expression medium supplemented with 8 mM L-glutamine (Thermo Fisher Scientific, MA) in 125-mL disposable polycarbonate Erlenmeyer flasks with vented caps (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples containing 25 µg of protein were resolved on 8-16% Tris-Glycine or 4-12% Bis-Tris gels (Thermo Fisher Scientific) at 150V for 45 minutes ...
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bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.