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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Fresh human NK cells were cultured for 3 days at a density of 1×106 cells/ml in RPMI 1640 medium (Gibco, Thermo Fisher) containing 10 ng/ml recombinant human IL-2 (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... We grew HL-60 cells to approximately 1 × 106 cells/ml and passaged the cells every 2-3 days in Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher Scientific) supplemented with L-glutamine and 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μg of the plasmid was transfected into 20 mL of Expi293F cells at 3 × 106 cells mL-1 using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The Calu-3 cells or primary NHBE cells were seeded in a 96-well culture plate (Nunc, ThermoFisher Scientific, San Francisco, USA) at a density of 5×104 cells/well ...
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bioRxiv - Cancer Biology 2023Quote: ... the pelleted cells were suspended in 3 ml of 1X Red Blood Cell (RBC) lysis buffer (ThermoFisher, Cat. No. 00-4300-54) and incubated on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Expi293F cells were diluted to a density of 3 million cells per mL and transfected using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cells were incubated shaking at 130 rpm at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2024Quote: Mid-log LdAQP-GFP cells at a density of approximately 3×106 cells per mL were incubated with Mitotracker (Invitrogen/Molecular Probes, #M7512) at a final concentration of 40 nM during 15 min at 27°C in culture medium without FCS ...
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bioRxiv - Immunology 2024Quote: ... staining using the T cell polarization panel required 3 steps: The cells were stained with a LIVE/DEAD marker (Fixable Blue, UV450, Thermo Fisher Scientific). Secondly ...
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bioRxiv - Cancer Biology 2024Quote: ... BTSCs were dissociated into single cell suspension and 3 x 104 cells were plated on 48- well plates coated with poly-D-Lysine (Thermofisher, #A38904-01). When cells adhered to the plate ...
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bioRxiv - Immunology 2024Quote: ... FITC-IgG+ and biotin-blocked Per-CP streptavidin negative cells from this gate was sorted into wells containing 3 µl chilled lysis buffer (0.5X Gibco PBS, 3 U/µl RNaseOUT and 10 mM DTT) for a subsequent reverse-transcriptase reaction in a 96 well PCR plate ...
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bioRxiv - Cancer Biology 2021Quote: ... YT and KHYG-1 cells was performed with the Pierce Cell Surface Protein Isolation kit (Thermo Scientific, cat#89881) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Cell surface proteins were biotinylated by a non-cell permeable EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) as described (Pusapati et al. ...
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bioRxiv - Immunology 2019Quote: ... the newly formed sgRNA (7.5 pmol sgRNA for 200,000 cells) was mixed with TrueCut v2 Cas9 protein (0.25 ul of Cas9 for 200,000 cells; #A36499, Thermo Fisher) and incubated in the cell culture incubator (at 37C ...
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bioRxiv - Biochemistry 2020Quote: ... and passaged every three days when viable cell density reached 2.5 x 106 cells/mL in chemically defined protein-free medium CDCHO (Invitrogen) supplemented with 25 µM methionine sulfoximine and 50 µg/L reduced menadione sodium bisulfite (rMSB ...
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bioRxiv - Immunology 2020Quote: ... One million cells from lung cell suspension were resolved on 4 – 12 % NuPAGE Bis-Tris protein gels (Thermo Fisher), then transferred onto a nitrocellulose membrane (Biorad ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration of both Light-cultured cells and Heavy-cultured cells was measured by BCA assay (23225; Thermo Scientific) and homogenates were mixed to a 1:1 ratio ...
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bioRxiv - Cancer Biology 2022Quote: Total protein was extracted from the cell lines and tissues using NP40 cell lysis buffer (Invitrogen™, CAT: FNN0021RIPA) with PMSF (Acros Organics ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell lysates were prepared by scraping cells with mammalian protein extraction reagent (MPER, Thermo Fisher Scientific, Waltham, MA) containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich Inc. ...
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bioRxiv - Immunology 2023Quote: ... YT and KHYG-1 cells was performed with the Pierce Cell Surface Protein Isolation kit (Thermo Scientific, cat#89881) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... nuclear or whole cell protein extracts were obtained from cells using NE-PER nuclear extraction kit (Thermo Scientific, 78835) or RIPA buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and/or ToPro-3-3 (1 μM; Thermo-Fisher Scientific, Waltham, MA) for 10 minutes at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was purchased from Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... #3: 5′-GAAUAUUGAACUGGAAGCAGCACAU-3′) were purchased as Stealth RNAi siRNAs from Invitrogen. The target sequences were designed using the Block-iT RNAi Designer tool (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... 20 mL of 3 mM of 3-deoxyadenosine (cordycepin; Thermo Fisher Scientific) was added to the buffer to obtain a final concentration of 0.6 mM (150 mg/L) ...
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bioRxiv - Biochemistry 2023Quote: ... 5’-UUCUCCGAACGUGUCACGUTT-3’ and 5’-ACGUGACACGUUCGGAGAATT-3’ using Lipofectamine RNAiMIX reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Day 3 SP34 (Invitrogen) with 5 ng/ml BMP4 ...
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bioRxiv - Neuroscience 2020Quote: ... 3’-Diaminobenzidine (Invitrogen, 750118) as a substrate ...
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bioRxiv - Neuroscience 2020Quote: ... 3 mM DTT (Invitrogen) and 40 units RNAse OUT (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μM MMC (ThermoFisher) for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: A 3% agarose (Invitrogen) gel solution was prepared in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... IL-3 (Life Technologies), Dexamethasone (Sigma) ...
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bioRxiv - Systems Biology 2020Quote: ... QuantStudio 3 qPCR (Thermofisher) with KAPA Library Quantification Kit (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO-2/3 (Invitrogen); β-Catenin (BD Transduction Laboratories) ...
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bioRxiv - Immunology 2022Quote: ... and 3% FBS (Invitrogen). Epidermal sheets were separated from the dermis after incubation for 45 min at 37°C in 2.4 mg/ml of Dispase and 3% FBS and the epidermis was further digested for 30 min in PBS containing 1 mg/ml collagenase D ...
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bioRxiv - Neuroscience 2022Quote: ... QuantStudio 3 from ThermoFisher was used ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... on QuantStudio 3 (ThermoFisher) and data were quantified by the 2-ΔΔCT method.
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bioRxiv - Biophysics 2023Quote: ... DiIC18(3) stain (Invitrogen). Transferrin from Human Serum ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 mL Trizol (ThermoFisher) was added to 1 mL of cellular PBS suspension in a 15 mL test tube ...
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bioRxiv - Biophysics 2023Quote: ... 3 mM DDT (Invitrogen), 1.5 µM of primers listed in Supplemental Table S6 ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Invitrogen, 16140071), 1% GlutaMAX ...
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bioRxiv - Genetics 2024Quote: ... 3% ES-FBS (Gibco), 0.1 mM β-Mercaptoethanol (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... Qubit 3 (Fisher Scientific) and 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... All iPSCs were generated by Sendai virus reprogramming and clonal expansion using the CytoTune – iPSC 2.0 kit (ThermoFisher) to deliver the reprogramming factors ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated for 2 hours with the virus and then washed twice with Neurobasal-A medium (Gibco) containing 2% B-27 (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified via qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) on a QuantStudio 3 (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA extractions of 80 μL of virus stocks lyzed with 240 μL of TRIzol LS Reagent (Invitrogen) were performed in triplicate with the Direct-zol RNA MiniPrep kit (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...