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Citations for Thermo Fisher :
2001 - 2050 of 2844 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were mounted on microscopy slides using GLOX anti-fade buffer containing 10 mM Tris pH 8 (Thermo Fisher, AM9856), 2x SSC (Ambion ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher) and loaded onto a MonoQ 5/50 GL column for anion exchange chromatography ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher), then concentrated to ∼0.5 mL and loaded onto a Superose 6 Increase 10/300 column pre-equilibrated in GF150 buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 50-100 μg of protein in 10-40 μl volume was electrophoresed through 8% or 12% Bolt Bis-Tris Plus gels (InVitrogen) in Bolt MOPS or MES SDS running buffer (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... and seeded as single cells at a concentration of 200K cells/cm2 in maintenance medium (Essential 8 medium, Thermo Fisher) supplemented with a rho kinase inhibitor (Stemgent ...
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bioRxiv - Molecular Biology 2022Quote: 20~60μg lysates from culture cells or mouse tissues were separated on 10% Bis-Tris or 3~8% Tris-Acetate Novex NuPAGE gels (Invitrogen) and transferred to nitrocellulose membrane following standard procedures ...
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bioRxiv - Neuroscience 2022Quote: ... in EER mode with a flux of 8 electrons/pixel/s and a Selectris-X energy filter (Thermo Fisher Scientific) with a slit width of 10 eV to remove inelastically scattered electrons ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T was co-transfected with lentiviral expression constructs (12 µg), viral packaging plasmid (psPAX2, 8 µg), and viral envelope plasmid (pMD2.G, 4 µg) using Lipofectamine 2000 (Invitrogen) following manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection was performed with a total of 12 μg of library and 8 μg of VSV-G using Lipofectamine 2000 (Invitrogen) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... The level of 8-Hydroxy-2’-deoxyguanosine (8-OhdG) – a proxy for oxidative DNA damage – was analyzed in corals using DNA Damage Competitive ELISA Kit (Invitrogen) and MyCurveFit.com software as described in (20).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Perfusion was subsequently changed to bicarbonate-buffered extracellular solution supplemented with the voltage- sensitive dye Di-8-ANEPPS at 3 µM (D3167, Invitrogen). Following dye loading for 4 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cell culture supernatants were collected and analyzed for IL-8 release using a commercially available ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and submerged in cutting buffer (8 mM Na2HPO4.2H2O, 2 mM KH2PO4, pH = 7.4) in the Micron HV650 (Thermo Fisher Scientific). Coronal sections of 60µm were cut using a frequency of 70Hz ...
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bioRxiv - Neuroscience 2024Quote: ... stock solution (8 mg/mL) was freshly prepared by dissolving RB powder in sterile saline (catalog number: NC9054335, Fisher Scientific) on the day of photothrombosis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were drawn after 0.5, 1, 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge ...
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bioRxiv - Cell Biology 2023Quote: ... The iPSCs were cultured in Essential 8 (E8) medium containing E8 supplement and 100 μg/mL Penicillin-Streptomycin (E8 complete medium, all from GIBCO, Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... Cells were grown for six days at 37°C with 8% CO2 in shaking conditions at 125 rpm according to the manufacturer’s protocol (Thermo Fisher). ExpiFectamine™ CHO Enhancer and ExpiCHO™ Feed were added 18 to 22 hours post-transfection to boost transfection ...
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bioRxiv - Developmental Biology 2024Quote: Monolayers and EBs from day 3 to day 8 were dissociated to single cells with TypLE 1X (Thermo Fisher, 12563029) for 5 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 1M cells were plated in 10cm non-TC treated plates in 8 mL RPMI with HEPES (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 4 million cells were plated in 10 cm non-TC treated plates in 8 mL RPMI with HEPES (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Genetics 2023Quote: ... Testes of 8 weeks old mice were ground under liquid nitrogen and RNA was extracted using Trizol (Thermo Fisher Scientific) according to the manufacturer’s instructions but using 80% EtOH during the wash step.
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bioRxiv - Physiology 2023Quote: Animals were culled using a schedule-1 method followed by injection of the common bile duct with 1 mg/mL collagenase NB 8 (Serva) in RPMI 1640 (Gibco) and pancreas dissection ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Immunology 2023Quote: ... hTCEpi cells were stimulated by KGMTM-2 containing 20% (v/v) sterile PAO1 supernatant for 8 hours before lysis by TRI reagent (Ambion). Total RNAs were purified using Direct-Zol RNA miniprep kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... these reactions were electrophoresed on a non-denaturing 8% PAGE gel alongside O’RangeRuler 10 bp DNA and GeneRuler 50 bp DNA ladders (Thermo Fisher), followed by SYBR gold staining ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mL Lysis Buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific), and a 45-s process in a FastPrep-24 (MP Biomedicals ...
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bioRxiv - Cell Biology 2023Quote: DRG were dissected from 8- to 10-wk-old C57BL/6J mice in cold Hank’s balanced salt solution (HBSS) (Life Technologies) or Dulbecco’s Modified Eagle’s medium (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... embryonic fetal lung lobes were incubated in Advanced DMEM/F12 medium supplemented with 8 U/ml Dispase (ThermoFisher Scientific, Gibco) for 2 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... embryonic fetal lung lobes were incubated in Advanced DMEM/F12 medium supplemented with 8 U/ml Dispase (ThermoFisher Scientific, Gibco) for 2 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 8 μm paraffin sections were prepared using a Leica rotary microtome RM2265 and transferred to polysine coated slides (ThermoFisher Scientific), dewaxed ...
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bioRxiv - Neuroscience 2023Quote: ... the hippocampus form 6-8 neonatal mice was collected from both hemispheres in Hank’s buffered saline solution (HBSS, Life Technologies). Tissue was incubated in 0.25% trypsin (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... malayi adult female tissue (see section “Parasite material and treatment”) was cryosectioned longitudinally to 8 µm thickness with a CryoStar NX70 cryostat (ThermoFisher). All sections (∼11-16 sections per block ...
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bioRxiv - Immunology 2023Quote: ... were distributed into four wells of a 24-well plate and activated for 8 days by adding prewashed anti-CD3/CD28 Dynabeads at a 1:1 bead-to-cell ratio (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated in 20 mL 33 loading buffer diluted to 13 in lysis buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific)) ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resuspended in 500µL of Sonication buffer (SDS 1%, 10mM EDTA, 50mM Tris HCl pH 8) with an anti-protease cocktail (A32965, ThermoFisher) and sonicated for 15” at 4°C (30s ON ...
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bioRxiv - Genomics 2022Quote: ... and resuspended in 1x lysis buffer (10mM Tris pH 8, 5mM EDTA, 0.5% SDS, 1x (0.4mg/ml) Proteinase K (Thermo Fisher Scientific) in water at 10M cells/800ul ...
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bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Stably transduced cell lines were generated by infection over-night in the presence of 8 μg/ml polybrene and 48 hours after infection were selected with 1 μg/mL Puromycin (Gibco) for three days ...
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bioRxiv - Cancer Biology 2023Quote: ... and then cells lysates were prepared using RIPA lysis buffer as described previously.30 Total proteins (8 µg/sample) were loaded onto 4%-20% Novex Tris-Glycine gels (Invitrogen), transferred onto polyvinylidene fluoride membrane ...
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bioRxiv - Microbiology 2023Quote: ... Fractions were collected every 10 seconds from 8 to 15.5 min and dried in a SpeedVac (SPD-1010-115, Thermo Fisher). Rhamnolipid methyl esters did not exhibit a strong UV absorbance profile ...
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bioRxiv - Biophysics 2023Quote: ... These cells were then permeabilised using 20 µM digitonin for 5 min and labelled with 8 µg/mL recombinant GST-2xFYVE (69) which was detected using a GST primary antibody (71-7500, Invitrogen) and a Goat anti-Rabbit Alex-aFluor647 secondary antibody (A-21245 ...
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bioRxiv - Cell Biology 2023Quote: ... were then seeded at a density of ~8 × 104 cells/cm2 in EpiLife medium containing 60 µmol/L CaCl2 (#MEPI500CA Gibco) and 1% Human Keratinocyte Growth Supplement (#S0015 Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were plated in 6 well plates at 8 x 10^5 cells / well with DMEM / 10% FBS / NEAA / Pen-Strep (Gibco). Cells growing at ∼70-80% confluency were transfected with 1 μg of the desired expression plasmid (Sigma Aldrich ...
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... T47D (ATCC HTB-133) or primary cells from human tumors were fixed by adding 8% PFA (Thermo Scientific cat#28908) directly to the samples (1:1 v/v with media ...
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bioRxiv - Biophysics 2023Quote: ... samples were mixed with 2X Novex Tris-glycine native sample buffer and loaded into NuPAGE 3-8% Tris-glycine gels (Invitrogen) with NativeMark unstained protein standard (Thermo) ...
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bioRxiv - Cancer Biology 2023Quote: ... We section organoid blocks at 8 µm and mount them on Superfrost Plus Microscope Slides (12-550-15, Fisher Scientific). Hematoxylin and eosin staining was performed on the parent tumor and the resulting organoids derived from tissue collection according to standard protocols ...
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bioRxiv - Genomics 2023Quote: ... medium containing 2 µg/ml puromycin and 8 µg/ml polybrene (HCT116-Cas9) and 1 µg/ml (iPSC-iCas9) or no (NPCs- iCas9) puromycin (GIBCO) was exchanged ...