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Citations for Thermo Fisher :
1801 - 1850 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... or 5 × 103 cells/mL (N. gruberi) in 100 µL of the indicated medium in white TC-treated 96-well plates (Thermo Scientific Nunc Microwell 136101). For assays under standard growth conditions (“+ glc”) ...
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bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
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bioRxiv - Cell Biology 2023Quote: A live/dead assay on the cell-laden GelMa constructs (n=6 per condition) was performed using a LIVE/DEADTM Cell Imaging kit (Invitrogen, Waltham, Massachusetts, United States) 144 h after Gel synthesis ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... Cell culture supernatants containing specific anti-N mAb were harvested and concentrated using Pierce™ Saturated Ammonium Sulfate Solution (Thermo Fisher Scientific, Waltham, MA). Biotinylation of the mAb was performed using a Biotin Conjugation Kit by following the manufacturer’s instruction (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Bulk RNA was isolated from TA muscles of five mice from each of the four treatment groups (n=20) using Trizol (ThermoFisher Scientific, Waltham, MA, USA) and established methods.32 A NanoDrop spectrophotometer was used to determine RNA purity ...
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bioRxiv - Biophysics 2023Quote: Human neuroblastoma cell line (SK-N-BE) stored at -80°C in freezing medium (RPMI Gibco® supplemented with 20% FBS Gibco and 10% DMSO), were quickly defrosted at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Sequence-verified Cas9-gRNA-Puro plasmids and pMA donor plasmid were co-transfected into in-house C57BL6/N 6.0 ESC using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) prior to selection with 2 μg/ml puromycin for 48 hours ...
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bioRxiv - Biochemistry 2023Quote: ... as three full experimental technical replicates (n=54) with ∼100 ng of peptides injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole-cell lysates were prepared from 5x106 cells treated with N-acetylcysteine (MilliporeSigma A7250) for 4 hours followed by stimulation/oxidation with 1 mM hydrogen peroxide (Fisher Scientific Canada, Mississauga ON) for 5 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... 200-300 µg of each sample were weighed into tin cups and analysed for δ¹³C and δ¹⁵N using an EA-Isolink coupled to an Advantage V IRMS via Conflo IV (all instruments Thermo Scientific, Bremen). Carbon and nitrogen isotopic ratios were assessed on the delta scale relative to international standards (i.e. ...
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bioRxiv - Cell Biology 2024Quote: ... neural induction was initiated by changing the culture medium to a 1:1 mixture of DMEM/N-2 (DMEM/F-12 + GlutaMAX (Life Technologies, cat# 31331-028); 1X N-2 (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Neuroscience 2020Quote: ... were quantified in autaptic neuronal cultures using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43, Thermo Fisher Scientific, Waltham, MA, USA), similarly as in our previous report (Kawano et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... the medium was replaced with 5 ml fresh medium and 50 or 100 μM 12-azidodo-decanoate (azidomyristate) (Click-IT™ Myristic Acid, Azide, ThermoFisher Scientific, Cat n° C10268) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2019Quote: ... with different spectral signatures selected for the detection of PfHRP2 and PAN-pLDH were washed with distilled water and activated with Sulfo-NHS (N-hydroxysulfosuccinimide) and EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (Pierce, Thermo Fisher Scientific Inc., Rockford, IL), both at 50 mg/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... Individual samples were then labeled with isobaric tags using commercially available Tandem Mass Tag™ 6-plex (TMTsixplex™) (Thermo Fisher Scientific, P/N 90061) or TMT11plex (TMT11plex™ ...
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bioRxiv - Cell Biology 2019Quote: ... 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA),3,3’-dihexyloxacarbocyanine iodide [DiOC6(3)] and N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,(acetyloxy)methyl ester (Fluo-4 AM) were purchased from Molecular Probes (Invitrogen, Eugene, OR, USA). Agarose was obtained from Lonza (Walkersville ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc., MA, USA) solution at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... All CAMP (Christie-Atkins-Munch-Peterson)-positive [22] bacterial isolates (n=58) were confirmed as GBS using a slide latex agglutination test (Streptex Latex Agglutination Test, ThermoFisher Scientific Inc., Waltham, MA, USA). GBS isolates were subcultured on blood agar to assess purity and inoculated on stab agars (SVA ...
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bioRxiv - Neuroscience 2022Quote: ... consisting of DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml 1% penicillin/ streptomycin (pen/strep; all Thermo Fisher Scientific, Waltham, MA, USA) + 20 ng/ml BDNF + 20 ng/ml GDNF + 20 ng/ml NGFb (all Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... collected as single cell suspensions using cell strainer and resuspended in sphere formation medium which was composed of 1:1 DMEM and F-12 nutrient mix supplemented with B-27 and N-2 (Thermo Fisher Scientific, Waltham, MA, USA). 1,000 cells were plated in each well of ultra-low attachment 6-well plates (Corning ...
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bioRxiv - Genetics 2022Quote: ... Over the course of the following 4 days, the colonies were gradually transitioned to neural induction medium (NIM) (consisting of DMEM/F12, 1% N-2 supplement (Fisher Scientific, Cat #: 17-502-048), MEM non-essential amino acids (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... per well were distributed in Ibidi® µ-slide 8-well chambered coverslips and stained with 0.8 μM of the membrane specific fluorescent styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64; Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 0 μM (control) ...
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bioRxiv - Microbiology 2021Quote: ... Mtb-SC and Mtb-AG-infected rabbit lungs (n=3 per group per time point) was isolated using TRIzol reagent (Life Technologies, Grand Island, NY, USA) as described earlier (5 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the Sensation Plus FFPE Amplification and WT Labeling Kit (P/N 703089, Rev.4 Thermo Fisher Scientific Inc., Life Technologies). GeneChip ST Arrays (GeneChip Human Gene 2.1 ST Array Plate ...
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bioRxiv - Cell Biology 2019Quote: ... methoxy]-2-oxyethyl] amino]-5-methyl-phenoxy] ethoxy]phenyl-N-[2-[(acetyloxy) methoxy]-2-oxyethyl]-(acetyloxy) methyl ester (Fluo-4/AM) were from Molecular Probes (Invitrogen, Eugene, OR, USA). M ...
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bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
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bioRxiv - Molecular Biology 2021Quote: ... and hNAA40 vectors served as templates to generate attB-flanked hNAA40 L(dTIS>CTC) and hNAA40S PCR products suitable for use in a Gateway® BP recombination reaction with a donor vector (pDONR221, Invitrogen, cat n°12536-017) thereby creating an entry clone ...
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bioRxiv - Plant Biology 2019Quote: ... pollinated stigma was incubated in FM™ 4-64 Dye (N-3-Triethylammoniumpropyl-4-6-4-Diethylamino Phenyl Hexatrienyl Pyridinium Dibromide, Life Technologies T3166, 8.23 μM) for five minutes and subsequently washed in 1/2 Murashige and Skoog basal medium containing 10 % (w/v ...
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bioRxiv - Biophysics 2022Quote: ... supernatant discarded and the cell pellet dissolved by suspension in 300 µL of N-PER™ Neuronal Protein Extraction Reagent (Thermo Scientific catalog number: 87792) lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... After staining of the transferred proteins with amido black the cleavage product was cut out for sequencing via N-terminal Edman degradation performed on a Procise Protein Sequencer 494C (Applied Biosystems, Foster City, United States).
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from hiPSC-derived cortical neurons at DIV8 (Q5, Q6, n=3 each group) using the miRVana miRNA isolation kit (#AM1560, Ambion, Thermo Fisher Scientific, Waltham, MA, USA) in accordance to their manuals ...
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bioRxiv - Bioengineering 2024Quote: A separate set of ATA samples (n=4 per group) were immersed in optimal cutting temperature compound (OCT; 4585, Thermo Fisher Scientific; Waltham, MA, USA) immediately after excision and frozen at 80◦C for fluorescent immunostaining ...
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bioRxiv - Cell Biology 2024Quote: ... Silencer® Select N°1 negative control siRNA or predesigned Silencer® Select siRNAs for ZNF827 (s45694, s45695, s45696) (Thermo Fisher Scientific, Waltham, MA, USA) were used for the transfection process ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR of the cDNA was performed using a primer set for the N gene of RSV (45) and ACTB (Applied Biosystems, TaqMan Assay ID: Hs99999903_m1) as a housekeeping gene ...
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bioRxiv - Cell Biology 2023Quote: ... Expression constructs were made by LR reaction using the following destination vectors: pDEST15 (for N-terminal GST-fusion in E. coli, cat#11802014, Thermo Fisher Scientific, Waltham, MA, USA) and pcDNA-DEST53 (for N-terminal GFP fusion in mammalian cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface CRT was detected using anti- CRT(N) or anti-CRT(Cmut) antibody (1:200 dilution) followed by staining with Alexa Fluor™ 647 conjugated secondary antibody (Invitrogen A21245, 1:2000 dilution). Total CRT in healthy donor platelets was stained with anti-CRT(N ...
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bioRxiv - Neuroscience 2023Quote: ... KSR medium was replaced by N2 medium (N2; DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml pen/strep [all: Thermo Fisher Scientific, Waltham, MA, USA]), supplemented with 2i + 3i in 25% increments every two days until day 10 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein band corresponding to Fragment 2 was cut out and subjected to N-terminal sequencing analysis (Matsudaira, 1987) on an automated protein sequencer (#Procise 491 cLc; Applied Biosystems, Foster City, CA, USA) using the standard pulsed-liquid program for PVDF-blotted proteins.
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bioRxiv - Plant Biology 2024Quote: Three replicates of 50 after-ripened mature seeds were sterilized as above and subsequently imbibed on moist nitrocellulose filter papers (Whatman n°1; Thermo Fisher Scientific; Waltham, MA, USA) placed on 55 mm Petri dishes containing solid MS/2 with or without 1 μM 2-cis ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells expressing TMPRSS2 proteins or its domains in 6-well plates were washed with PBS and incubated with EZ-Link™ Sulfo-N-Hydroxy Succinimide– SS-Biotin (0.25 mg/ml, 1 ml/well) (Thermo Fisher Scientific; Cat No: A44390) label for 20 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: PCL nanofibrous sheets were cut into discs (16 mm diameter, n=24) using an Acuderm Acu-Punch Biopsy Punch® (Acuderm Inc., Fisher Scientific Ltd., Loughborough, UK) and inserted into a 24-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... KSR medium was replaced in 25%-steps with N2 medium (N2; DMEM/F12 GlutaMAX, 1X B-27 Plus Supplement, 1X N-2 Supplement, 100 U/ml penicillin/streptomycin [all: Thermo Fisher Scientific, Waltham, MA, USA]) every other day ...
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bioRxiv - Molecular Biology 2020Quote: ... The test was carried out at the optimized temperature determined as described above with four positive controls (1 pg pUC57-N DNA) and four negative controls (ddH2O) in a StepOne™ System (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Cancer Biology 2019Quote: Treated cell-pellets were lysed and digested using the commercially available Pierce™ Mass Spec Sample Prep Kit for Cultured Cells (Thermo Fisher Scientific, P/N 84840) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) (1 ...