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Citations for Thermo Fisher :
1751 - 1800 of 1859 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCRs were performed in technical and independent experiment triplicates (n=3) with Power Sybr Green Master Mix (Life Technologies, Darmstadt, Germany) on a VIIA7 (Life Technologies ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: MARC-145 cells transfected with the recombinant pCAGGS-N plasmid were lysed in Pierce™ IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA), and then mixed with each of the purified anti-N mAbs ...
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bioRxiv - Microbiology 2023Quote: ... Total cDNA was obtained by reverse transcription using random hexamers pd(N)6 and M-MLV reverse transcriptase (Life Technologies, Carlsbad, CA, USA) at 42 °C for 50min ...
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bioRxiv - Immunology 2023Quote: Open-reading frames coding for either Nefmut alone or fused with SARS-CoV-2 N protein were cloned into pVAX1 plasmid (Thermo Fisher, Waltham, MA) as previously described [20] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: The RBD protein was incubated for 30 min at 21 °C and 750 rpm with a 10 molar equivalent of sulfo-m-maleimidobenzoyl-N-hydoxysuccinimide ester (Sulfo-MBS) (Thermo Scientific; MW 416.30). The excess crosslinker was removed by passing the protein through a PD-10 column using a PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:30 rat anti-N-cadherin (Developmental Studies Hybridoma Bank, DN-EX #8,) and 1:200 rabbit anti-lucifer yellow (Molecular Probes, A-5750). The secondary antibodies used were 1:500 goat anti-rabbit with Alexa fluor 633 (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS- CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described (73 ...
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bioRxiv - Biochemistry 2023Quote: ... n=4) collected in microcentrifuge tubes were subjected to a 30-min incubation in 30 μM iodoacetamide (IAM) (Thermo Fisher Scientific, Waltham, MA) or N-ethylmaleimide (NEM ...
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bioRxiv - Immunology 2023Quote: ... The peptides were separated at a flow rate of 300 nL/min on C18 pre-column (Acclaim PepMap™ 100, 75 µm × 2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMap™ RSLC C18 ...
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bioRxiv - Genomics 2023Quote: ... Cells were then plated onto Geltrex-coated surfaces at 1.5×105 cells/cm2 in the induction medium (DMEM/F12 supplemented with 1× N-2 (Thermo Fisher Scientific, cat# 17502048), 1× non-essential amino acids (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 568 conjugated secondary antibodies (Invitrogen; 1:1000 in blocking buffer). Actin tails were labeled with Alexa Fluor 488 phalloidin (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The N concentrations were determined by dry combustion of a 3-4 mg sample with a Flash EA1112 elemental analyzer (Thermo Scientific, Rodano, Italy).
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bioRxiv - Biochemistry 2022Quote: ... The peptides were separated at a flow rate of 300 nL/minute on C18 pre-column (Acclaim PepMap™ 100, 75 μm□×□2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMap™ RSLC C18 ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from the LV myocardium of 2-month-old male WT and Rbm20ΔRS mice (n = 3 per group) using TRIzol Reagent (Life Technologies, Waltham, MA, USA) and treated with DNase I (cat# D9905K ...
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bioRxiv - Plant Biology 2023Quote: ... Equal amount of protein samples were electrophoresed on 10 % NuPAGE® Bis-Tris Gels in 1X MES [2-(N-morpholino) ethane sulfonic acid] SDS buffer (Invitrogen, Waltham, MA) and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: Total protein from muscle tissue samples (n = 6 for EarlyMT and n = 16 for LateMT) was extracted using an extraction buffer containing Tissue extraction Reagent I (FNN0071, Invitrogen, Waltham, Massachusetts, USA), HALT Protease and Phosphatase Inhibitor Cocktail (1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
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bioRxiv - Neuroscience 2023Quote: ... and maintained in N2B27 media (1:1 of DMEM F12 Glutamax [Thermo Fisher Scientific, 10565018] and Neurobasal media [Thermo Fisher Scientific, 12348017], 1X N-2 (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: NHR2 labeling was performed with the reactive Alexa Fluor® 488 dye using N-hydroxysuccinimide (NHS)–ester chemistry (NHS Ester Protein Labeling kit, ThermoFisher Scientific, USA), which reacts efficiently with primary amines of proteins to form stable dye– protein conjugates ...
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bioRxiv - Molecular Biology 2024Quote: ... and mouse (n=6; kept on HFD for 28 weeks) livers were measured with the GeneChipTM miRNA 4.0 Array (Applied Biosystems, Foster City, US).
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bioRxiv - Cell Biology 2024Quote: ... GEMs were then broken and the single-stranded cDNAs were cleaned up with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific; P/N 37002D). The cDNAs were PCR amplified ...
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bioRxiv - Neuroscience 2024Quote: ... non-metabolizable glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Thermo Fisher Scientific, cat.no. 11569116). Samples were prepared as described in the section ...
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bioRxiv - Microbiology 2024Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify genomic RNA of SARS-CoV-2 N gene using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described2 using the following primers and probes ...
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bioRxiv - Cell Biology 2019Quote: ... First antibody incubation was done O/N at 4°C and the secondary HRP-coupled antibodies goat anti-Mouse IgG (H+L) (ThermoFisher scientific; 31430; 1:10,000) or polyclonal HRP-conjugated goat-anti-rabbit IgG (ThermoFisher scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to a Q Exactive hybrid mass spectrometer (Thermo) (N-terminal FLAG-tagged datasets) or an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer (C-terminal FLAG-tagged datasets) ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Physiology 2022Quote: Samples of frozen mouse liver were homogenized in lysis and extraction buffer (RIPA lysis and extraction buffer, catalog n° 89900, Thermofisher Scientific, Waltham, MA, USA) containing protease inhibitors (protease inhibitor cocktails tablets ...
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bioRxiv - Physiology 2022Quote: ... equal amounts of the protein samples (20 μg of liver extracts) were resuspended in Laemmli sample buffer (catalog n°39000, Thermofisher Scientific, Waltham, MA, USA) and separated in an 4%–20% sodium dodecylsulfate (SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... the inserts of the entry vectors were recombined into the into the pDEST 5’ BirA*-FLAG-pcDNA5-FRT-TO destination vector (Invitrogen, cat n°12285-011) using LR-clonase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... [30] at a concentration of 57.5 μM (5% of the suggested concentration in RPMI 1640 medium) and media were supplemented with 10% dialyzed fetal bovine serum (Invitrogen, cat n°26400-044). Cell populations were cultured for at least six population doublings for complete incorporation of the labelled L-Arg.
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bioRxiv - Biophysics 2022Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Catalog No. 22980) and Sulfo-NHS (N-hydroxysulfosuccinimide, Catalog No. 24510) were purchased from Thermo Scientific (Waltham, MA, US). Phosphate-Buffered Saline (PBS ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies, Darmstadt, Germany). Fold expression changes were determined using a standard 2−ΔΔCT method that compares CT (cycle threshold ...
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bioRxiv - Developmental Biology 2019Quote: ... C- and N-terminal GFP-tagged Nubp1 and Nubp2 expression plasmids [32] were transfected using Lipofectamine® 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Nikon C2 Confocal microscope.
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bioRxiv - Physiology 2019Quote: ... Purified RNA (100 ng per sample) was labeled with the Whole-Transcript Sense Target Assay kit (Affymetrix, Santa Clara, CA, USA; P/N 900652) and hybridized to an Affymetrix Mouse Gene 1.0 arrays or 2.1 ST array plate (Affymetrix) ...
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bioRxiv - Neuroscience 2019Quote: ... cycling synaptic vesicles were stained with the styryl dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino) styryl) pyridium dibromide (FM1-43; Molecular Probes, Inc., Eugene, OR). For labeling of synaptic vesicles ...
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bioRxiv - Biophysics 2021Quote: ... The silanized surfaces were incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2022Quote: The reverse transcription was performed with 500 ng of total RNA using the High-capacity cDNA reverse transcription kit (ThermoFisher Scientific, catalog n°4368814) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... A nanoViper Trap Column (C18, 3 μm particle size, 100 Å pore size, 20 mm × 75 μm ID; Thermo Fisher Scientific, P/N 164946) was used for desalting ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were run on a PepMap RSLC C18 column (2 μm particle size, 15 cm × 50 μm ID; Thermo Fisher Scientific, P/N ES901). A nanoViper Trap Column (C18 ...
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bioRxiv - Biophysics 2022Quote: ... was spiked with 1% of Alexa 488-labeled HU-B mutant (containing an N-terminal Cys conjugated with Alexa Fluor™ 488 C5 Maleimide from Thermofisher; Cat no. A10254). The 488 nm laser was set at 100% for efficient bleaching and focused on a small circular region with a diameter of 1 μm ...
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bioRxiv - Developmental Biology 2019Quote: ... The silicon device surfaces were functionalized with a previously described protocol using 3-mercaptopropyltrimethoxysilane (MPTMS) and N-gamma-maleimidobutyryloxysuccinimide ester (GMBS) NeutrAvidin-biotin chemistry (Thermo Fisher Scientific, Rockford, IL)[32 ...
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bioRxiv - Developmental Biology 2020Quote: The retinas of juveniles (n = 6 retinas from 5 animals) and adults (n = 5 retinas from 4 animals) were dissected out and put in RNAlater (Ambion Inc., Austin, TX, USA). RNA extraction was performed using the RNeasy mini kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Genetics 2020Quote: Guinea pig anti-zebrafish Rad21l1 polyclonal antibody production: An N-terminal fragment of Rad21l1 cDNA was amplified with Phusion DNA polymerase (Thermo Fisher Scientific, Catalog #: M0530L) using the following primers ...
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bioRxiv - Plant Biology 2021Quote: ... C and N concentrations (mass based) of the samples (5 mg dry weight) were determined with Elemental analyzer (Flash EA 2000, Thermo Fisher Scientific, Bremen, Germany) in four biological replicates ...
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bioRxiv - Cell Biology 2020Quote: ... n-dodecyl-β-D-maltoside and assessed for OxPhos complex content using a Native PAGE Novex Bis-Tris Gel system (Invitrogen, Carlsbad, CA, USA). The separated proteins were transferred to polyvinylidene fluoride membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... for amplification of 100ng of cDNA template from N/D/S cells either treated with MS351 or vehicle using Power SYBR Green Master Mix (Thermo Fisher Scientific Cat. 4368577) qRT PCR protocol14 ...
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bioRxiv - Cancer Biology 2022Quote: ... with or without N-ethyl-N-nitrourea 10μg/ml or 50μg/ml and treated 2h with 1mg/ml Collagenase type IV (Gibco by life technologies ref 17104-019).
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bioRxiv - Neuroscience 2023Quote: ... for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse ...