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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UKEi1-A was reprogrammed with a Sendai Virus strategy (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). These studies were approved by the Ethical Committee of the University Medical Center Hamburg-Eppendorf (Az ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from 1010 virus using the PureLinkTM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The virus was dialyzed with PBS supplemented with 0.05% of Pluronic F68 (Thermo Fisher Scientific) using 100-kDa filters (Millipore) ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus (MMLV) reverse transcriptase (#28025013, Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Plant Biology 2019Quote: ... 10 IU of Moloney murine leukemia virus RNase H+ reverse transcriptase solution (Thermo Fisher Scientific), and appropriate buffer containing deoxynucleoside triphosphates (dNTPs ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated total RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo-dT primers ...
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bioRxiv - Microbiology 2021Quote: ... MRC-University of Glasgow Centre for Virus Research) were maintained in Leibovitz’s L15 media (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: Compound dilutions for virus treatment were performed in Minimum Essential Media (MEM) purchased by Gibco.
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bioRxiv - Microbiology 2019Quote: Concentrated virus aliquots were mixed with DiI (final concentration of 1 μM, Thermo Fisher, USA), vortexed and incubated for 1.5 h at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher) and 5 µl of isolated RNA as a template to detect a 132 bp sequence in the ORF1b/NSP14 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... expanded PBMCs were transduced with non-integrating Sendai reprogramming virus (Cytotune 2.0, Thermo Fisher, A16517) according to manufacturer’s instructions added to the Expansion Media ...
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bioRxiv - Neuroscience 2021Quote: ... The viral DNA samples were prepared for quantification by treating the virus with DNaseI (Invitrogen) and Proteinase K ...
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bioRxiv - Microbiology 2020Quote: The nasal swab was initially stored in virus transport medium [Hank’s Balanced Salt Solution (Gibco) containing 2% FCS (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... qPCRs were set up using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher) and run on an ABI 7500 Fast Real-time PCR System (Applied Biosystems/Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... the virus inoculum was previously activated with trypsin (200 μg/ml, Gibco Cat. 27250-18) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The Virus was washed and eluted in Hank’s balanced salt solution (Gibco™, Dublin, Ireland) containing 0.014% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: Purified virus stocks were diluted in lithium dodecyl sulfate (LDS) sample buffer (Thermo Fisher Scientific) and separated by SDS-PAGE (NuPAGE Novex 10% Bis-Tris gel ...
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bioRxiv - Neuroscience 2022Quote: ... and reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time polymerase chain reaction (PCR ...
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bioRxiv - Immunology 2023Quote: ... vRNA was reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 instrument (Roche ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the precipitated virus pellet was resuspended with 1.1 mL of Opti-MEM (Gibco #31985062) by pipetting and vortexing until fully dissolved ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed with TaqMan Fast Virus 1-Step Master Mix (Invitrogen, cat. 4444434) in QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... Virus samples at variable dilutions (1:10 to 1:40,000) were mixed with FluoSphere (Invitrogen) beads (170nm ...
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bioRxiv - Microbiology 2024Quote: ... The virus genome was further amplified by PCR (Phusion High-Fidelity DNA Polymerase, ThermoFisher Scientific) with 10 μL cDNA in each 50 μL reaction and MBTuni-12/13 (5′-ACGCGTGATCAGTAGAAACAAGG ...
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bioRxiv - Biochemistry 2024Quote: ... Virus generation was performed using the Bac-to-Bac™ expression system (Thermo Fisher Scientific) in Sf9 insect cells ...
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bioRxiv - Neuroscience 2020Quote: We dissected brains and ventral nerve cords of 5-7 days old males in Schneider’s insect medium (Thermo Fisher Scientific, Waltham, MA) and fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2020Quote: ... Bovine blood intake by insects was determined using a SYBR green assay (PowerUp™ SYBR™ Green Master Mix, A25779, Life Technologies) for the detection of bovine mitochondrial cytochrome b as described by Van Der Saag et al ...
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bioRxiv - Cancer Biology 2020Quote: ... Red cells were removed by incubating the splenocytes for 3 minutes with 3 ml eBioscience™ 1X RBC Lysis Buffer (Invitrogen, ThermoFisher, #00-4333-57). Cells were pelleted by centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Cell Biology 2022Quote: ... cells were prepared using the Subcellular Protein Fractionation Kit for Cultured Cells (78840 from ThermoFisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Cells were lysed and proteins were extracted from fresh fibroblast cells with RIPA buffer (Thermo Scientific) supplemented with protease and phosphatase inhibitor mini-tablet (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 40 μM strainer (Flowmi) and counted on an automatic cell counter (Countess 3, Invitrogen) immediately before loading onto the 10X Chromium machine ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were subcultured every 3-4 days as follows: cells were washed with phosphate buffered saline (PBS, Life Technologies) and then incubated with trypsin (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... A fluorescence-based cell viability assay was performed 3 days after plating using AlamarBlue cell viability reagent (Thermo Fisher). Fluorescent signals were normalized to background signals from wells plated with Matrigel only.
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bioRxiv - Microbiology 2022Quote: ... were seeded with PD07i epithelial bladder cells at a concentration of ~ 3×106 cells in EpiLife Medium (Gibco, #MEPI500CA) supplemented with growth supplements and antibiotics (HKGS ...
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bioRxiv - Microbiology 2022Quote: ... were seeded in T-25 flasks at a cell density of 3 × 106 cells in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Biowest) ...
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bioRxiv - Physiology 2022Quote: ... Cells were fixed with ice-cold 4% paraformaldehyde solution and cell nuclei were stained with To-Pro-3 (Invitrogen). Samples were mounted with Mowiol (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Differential cell counts (∼400 cells/slide) were performed on cytospin-prepared slides stained with PROTOCOL Hema 3 (Fisher Scientific). Total cell counts are expressed per microliter of BALF.
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bioRxiv - Immunology 2023Quote: ... Cells were then resuspended in cRPMI and counted using the Countess 3 FL Automated Cell Counter (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then resuspended in 200 μl of PBS and counted on a Countess 3 automated cell counter (Invitrogen). All centrifugations were done at 400g for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was determined using Trypan Blue staining and counted using an automated cell counter (Applied Biosystems Countess 3). Cells were centrifuged at 300 x g for 5 minutes and resuspended in the appropriate volume of resuspension buffer (HBSS + 0.04% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed 3 times in 5% Bovine Serum Albumin (BSA; ThermoFisher Scientific, 11423164) in PBS then incubated with primary antibody to H2AX (Ser139) ...
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bioRxiv - Genetics 2021Quote: ... Calu-3 cells were maintained in MEM α media supplied with 10% FBS (Gibco) and 1% Pen/Strep (0.025% ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split every 3 days at 1:10 using Trypsin-EDTA (ThermoFisher Scientific). On day 1 ...
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bioRxiv - Cell Biology 2022Quote: PC-3 cells (ECACC 90112714) were maintained in RPMI medium (GIBCO, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: PC-3 cells (ECACC 90112714) were maintained in RPMI medium (GIBCO, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... The Calu-3 cells were cultured with MEM (Thermo Fisher Scientific, Waltham, MA, USA) containing 20% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were fixed and stained using the Protocol Hema 3 staining kit (Fisher Scientific). Colony number and size were determined using the Fiji Is Just ImageJ (Fiji ...
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bioRxiv - Microbiology 2019Quote: Calu-3 epithelial cells (ATCC HTB-55) were maintained in optiMEM medium (Life Technologies) supplemented with 5% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged every 2-3 days by washing with HBSS (Gibco, cat#14170), trypsinizing using 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Physiology 2021Quote: ... cells were fed every 2-3 days with DMEM + 10% FBS (Thermo fisher Scientific) until differentiation was complete at day 10.