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Citations for Thermo Fisher :
1601 - 1650 of 6146 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded onto coated 35 mm glass bottom dishes with a 20 mm glass aperture (Fisher Scientific D35-20-1.5-N). HEK293A cells were seeded onto dishes that had been coated with 10 µg ECL cell attachment matrix (Sigma 08-110 ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody-mediated inhibition of hNR-EVs was performed with a commercial antibody that recognizes an extracellular topological domain of PLP1 at its N terminus (AA 36 to 85, Invitrogen, PA5-40788) incubating hNR-EVs with anti-PLP1 1.25 ug/ml for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... and also cultured16 the fluid by spread-plating 20 microliters onto a pre-reduced tryptic soy 5% defibrinated sheep blood agar (80% N–10% H– 10% CO2, at 37°C; Thermo Fisher Scientific) using a variable-atmosphere anaerobic Whitley workstation A85 (540 plate capacity ...
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bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
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bioRxiv - Plant Biology 2024Quote: ... Contents of C and N were determined from 5 mg of tissue powder on a Flash 2000 Elemental Analyzer (Thermo Fisher Scientific), and contents of S were determined from 20 mg of tissue powder mixed with 5 mg of tungsten trioxide on an elemental PYRO cube analyzer (Elementar) ...
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bioRxiv - Neuroscience 2024Quote: A total of 12 WT and pink1 KO mice (N = 3 per sex/genotype) at age of three months were anesthetized and intracardially perfused with cold PBS (Invitrogen, 14190-235). Brains were removed and immediately flash-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were loaded on a trapping column and eluted over a 75μm x 50cm analytical column (Thermo Fisher P/N ES-803) at 300 nL/min using a 1hr reverse phase gradient ...
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bioRxiv - Neuroscience 2024Quote: ... and granulocyte-macrophage colony-stimulating factor (GM-CSF, 10 ng/ml) to DMEM/F12 (HyClone™, SH30023.01) containing N-2 supplement (Gibco™, 17502048). This protocol facilitated the maturation process of the PMPs into microglia.
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bioRxiv - Plant Biology 2023Quote: ... L−1 of 3’-biotin-labeled primer probe purified by high-pressure liquid chromatography (HPLC) for 4-12 h at 42°C in Hybaid Shake ‘n’ Stack (Thermo Fisher Scientific) with gentle rotation ...
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bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco® ...
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bioRxiv - Molecular Biology 2024Quote: ... level was measured from the plasma of representative subsets of the groups (n=16) using an automated analyzer (Indiko Plus, Thermo Fisher, US).
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Biochemistry 2024Quote: ... with a replacement in the N-terminal domain loop 8 (139CQKRDGPH146 to 139AEAG142, designated as rA3GR8) was constructed in the pET-sumo vector (Thermo Fisher Scientific). This construct generated a SUMO fusion with an N-terminal 6xHis tag and a PreScission protease cleavage site ...
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bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
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bioRxiv - Bioengineering 2024Quote: ... which in turns intercalates with nucleic acids and emits red fluorescence detectable by microscopy.38 Frozen tissue sections from ATA samples (n=4 per group) were stained with DHE (10µM; D1168, Invitrogen; Carlsbad, CA, USA) in a dark humidified incubator at 37◦C for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were first concentrated over an Acclaim PepMap trap column (5.0 µm C18-coated particles, 0.5 cm * 300 µm ID, ThermoFisher Scientific P/N 160454), then bound onto a PepSep XTREME column (1.5 µm C18-coated particles ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) (Thermo Scientific) and 1 mM isopropyl beta-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2019Quote: ... diphenylene iodonium (DPI, an inhibitor of reduced nicotinamide adenine dinucleotide phosphate oxidase, 10 μM, Molecular Probes), or DNase I (10 μM ...
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bioRxiv - Microbiology 2020Quote: ... and 1:1 3-methyl-1-butanol (Thermo Fisher Scientific) in mineral oil were used as cues ...
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bioRxiv - Microbiology 2020Quote: The ΔΨ component was measured using tetramethylrhodamine methyl ester (Invitrogen) as described previously14 ...
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bioRxiv - Molecular Biology 2021Quote: ... BODIPY TR methyl ester (1:300, Life Technologies, Darmstadt, Germany) or Vybrant DiO (1:200 ...
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bioRxiv - Physiology 2020Quote: ... Sections were stained with BODIPY TR methyl ester (Thermo Fisher) diluted 1:200 ...
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bioRxiv - Genetics 2021Quote: ... using a Leica M205C stereomicroscope (190-240X magnification) and collected directly into sterile 0.2 mL PCR flat-cap tubes containing Prep-n-GoTM Lysis buffer (ThermoFisher Scientific, Carlsbad, CA, USA). This was done via a sterile tungsten needle and water-soluble 3MTM adhesive ...
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bioRxiv - Microbiology 2022Quote: ... and expression clones with N-terminal fusion tags were produced simply by Gateway cloning (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen, 11791020). The Lenti-NSP2 was constructed by using the destination vector ...
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bioRxiv - Neuroscience 2021Quote: ... and for bank vole cells NBM complete was switched to DMEM complete supplemented with 1% N-2 supplement (Gibco™ Thermo Fisher, USA). Mouse or bank vole glia cells were plated at 1×105 or 5×104 cells/well ...
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bioRxiv - Neuroscience 2021Quote: ... and for bank vole cells NBM complete was switched to DMEM complete supplemented with 1% N-2 supplement (Gibco™ Thermo Fisher, USA). Mouse or bank vole glia cells were plated at 1×105 or 5×104 cells/well ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated using 500 ng of total RNA and the High-Capacity cDNA with Reverse Transcription kit (Applied Biosystems p/n 4368814). QPCR was performed using the Wafergen SmartChip system (TakaraBio ...
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bioRxiv - Immunology 2019Quote: ... The reaction was stopped with 3 N H2SO4 and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa).
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bioRxiv - Genomics 2019Quote: ... Samples were heat-denatured (95°C/ 5 min) and incubated o/n at 37°C with 150 U of λ-exonuclease (λ-exo; Thermo Scientific) in the presence of 40 U of RNAseOUT ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The LCRN coding sequence was inserted in frame with a N-terminal hexahistidine (His6)-SUMO tag in a Champion pET SUMO expression vector (Invitrogen, Carlsbad, CA, USA) by Gibson Assembly (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... we designed construct PolI-SARS-CoV2-NLuc-N-HDR and ordered this as insert in the pMK-RQ backbone (Invitrogen construct ID 20ACX40P). The resulting plasmid pPolI-SARS-CoV2-NLuc-N was digested with XbaI prior to transfection to eliminate background (read-through ...
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bioRxiv - Cell Biology 2021Quote: ... #ACS-1024) and 9-1 (UMN PCBC16iPS/vShiPS 9-1)25 were expanded in culture on vitronectin (VTN-N, Thermofisher Scientific, Cat. #A14700) in Essential 8 Medium (E8 ...
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bioRxiv - Neuroscience 2019Quote: ... HPLC-purified dipeptide repeats were purchased from Pepscan and were either labelled with a single Cy5 dye at the N-terminus during synthesis or were labelled non-site-specifically in-house using Alexa488-Fluor® Protein Labeling Kit (Thermo Fisher Scientific) using published methods (94).
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bioRxiv - Plant Biology 2020Quote: The coding DNA sequence for human Factor IX without the native signal peptide was synthesized as N-terminal translational fusion to the signal-peptide coding sequence of PpAP1 (Pp3c5_19520V3.1)42 by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA). It was cloned into the pAct5-MFHR1 plasmid41 to generate the final FIX expression vector ...
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bioRxiv - Immunology 2021Quote: ... A tPA signal was added at the N-terminus to facilitate protein secretion and the construct was cloned into pcDNA3.4 vector (Thermo Fisher, Waltham, MA, USA) which drives transcription via a CMV promoter ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into the pFastBac1 vector with a 10xHis tag appended at the N terminus and was expressed in Sf9 cells (Gibco, catalog number 12659017). The cells were cultured at 27 °C for 60 h after being infected with baculoviruses using the Bac-to-Bac system (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for an additional two hours with Alexa 594 streptavidin-conjugated antibody (1:500, #S32356, RRID: N/A, Thermo fisher Scientific, USA). Sections were imaged after rinsing ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were targeted in a second bioreactor sampling experiment (n=2 for both bioreactors) for which the Plasmid Miniprep kit was used according to manufacturer’s instruction (Thermo Fisher Scientific, Waltham, US), with the addition of a step homogenizing the cells before processing ...
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bioRxiv - Cell Biology 2019Quote: ... This exogenous N-terminal GFP fusion was made by first cloning TtPOC5 into the pENTR4 Dual Selection Entry Vector (Invitrogen, Carlsbad, CA, USA) and then using the Gateway cloning system (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated at 4°C overnight with the primary antibodies (Mouse anti-Dyrk1a: Abnova, Cat. N. H00001859-M01; Rabbit anti-Camk2a: Molecular probes, PA5-14315). After washing ...
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bioRxiv - Plant Biology 2021Quote: ... A 0.5 g sample of the collected tissues were ground under liquid nitrogen and suspended in 1 ml of co-IP extraction buffer (1× PBS, 1% n-dodecyl-β-d-maltoside (Invitrogen, Carlsbad, CA), protease inhibitor cocktail cOmplete™ (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... GEMs were broken and the single strand cDNA was cleaned up using DynaBeads® MyOne™ Silane Beads (Life Technologies, P/N 37002D). cDNA was amplified using a Bio-Rad PTC-200 Thermal cycler with 0.2ml 8-strip non-Flex PCR tubes ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (n = 500 000 cells/well) were transfected with 25 nM of each siRNA individually using Lipofectamine® 3000 Transfection Reagent (ThermoFisher Scientific, # L3000075) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The distillates from the KCl extracts were quantified by titration and then acidified and oven-dried at 60 °C for N isotope analysis by mass spectrometry (Finnigan MAT251, Thermo Fisher, Waltham, MA) according to Hauck et al ...