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Citations for Thermo Fisher :
1451 - 1500 of 6146 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... the tissue was stained with NeuroTrace™ green fluorescent Nissl stain (1:200 in PBS, Life Technologies, catalog No. N-21480) for 20 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... OLV-FL or one of the truncated OLV versions were shuttled simultaneously with PYE into the destination vector pBiFC-2in1-NN (Grefen and Blatt, 2012) (N-terminal nYFP and cYFP fusions) via multisite LR reaction (Gateway, Thermo Fisher). Hereby pBiFC-2in1-NN:OLV-FL-PYE and pBiFC-2in1-NN:PYE-OLV-FL (additionally all truncated OLV versions were cloned into pBiFC-2in1-NN combined with PYE as described for OLV-FL ...
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bioRxiv - Plant Biology 2023Quote: ... For N-terminal fusions the pDONR entry clones with PYE and OLV sequences were used for Gateway LR reaction (Gateway, Thermofisher, Scientific) to generate the destination vectors based on pH7WGY2 (N-terminal YFP ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Neuroscience 2023Quote: The forebrain sections were incubated with Blue NeuroTrace Fluorescent Nissl Stains (1:100 dilution by PBS, N-21479, Thermo Fisher Scientific) at room temperature for 2-3 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Dried lysed cellular lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Neuroscience 2023Quote: ... then reverse transcribed using random primers with a N-terminal amine group and Revert-aid H-minus reverse transcriptase (Thermo Fisher). On the second day ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Bioengineering 2023Quote: ... and 474/238), or fetal bovine serum (FBS, n = 542/415, 384/184, and 459/308) using a live/dead kit (Thermofisher, #L3224). Cell suspensions were centrifuged ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Thermo Fisher Scientific, 17502-048], 1X B-27 [Thermo Fisher Scientific, 17504-044], 1mM L-Glutamine [Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Dried cell lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 300g for 5 minutes at 4°C and homogenized in cold N-PER Neuronal Protein Extraction Reagent (Thermo Fisher) or cold RIPA lysis buffer (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... five 3rd instar larval brains were dissected and homogenized in 30 μl lysis buffer (N-Per lysis buffer (Thermo Fisher, 87792) containing 0.1 mM DTT and 1:100 diluted proteinase inhibitor cocktail (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... blots were incubated with primary antibodies (HA-Cell Signaling: #2367; Hsp90-Thermo Fisher: MA1-10372; N-cadherin-Thermo Fisher: 33-3900) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: HEK293T cells were transferred with C-terminal HA-tagged Kayak and N-terminal GFP-tagged Jun-epi or Kayak using Lipofectamine 2000 (11668030, Thermo Fisher). The GFP-trap agarose beads (ABIN509397 ...
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bioRxiv - Immunology 2024Quote: Plasmodium falciparum 3D7 CSP was used as a reference to generate an N-terminal immunogen conjugated to KLH (Thermo Fisher Scientific). Murine studies were performed under approved protocol 22-0007 ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Immunology 2023Quote: COS-7 cells were cultured for 12 h on glass coverslips in a 24-well plate and then co-transfected with pcDNA3.1-NOX2-N-Clover and EROS-C-mRuby2 using Lipofectamine 3000 (Thermo Fisher Scientific) for 24 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... zebrafish larvae from visually similar developmental stages were collected in groups of 16 individuals from all corresponding genotypes (N = 4-5 per genotype) and stored in RNAlater (Thermo Fisher) until use ...
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bioRxiv - Neuroscience 2024Quote: ... The cell culture medium was removed and cells were suspended with 1 mL ice-cold PBS and then treated with 1 mL sulfo-N-hydroxysuccinimide-SS-biotin (Thermo Scientific) (1 mg/mL in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Neuronal differentiation was induced by culturing NGN2-NPC in neural differentiation medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco® ...
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bioRxiv - Neuroscience 2024Quote: PVN tissue biopsies were collected from fresh-frozen brain tissue and protein extracted using Neuronal Protein Extraction Reagent (N-PERTM, Thermo Scientific) for determination of CRH protein levels by Western Blot ...
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bioRxiv - Cell Biology 2024Quote: ... Cnn or TACC CDS were recombined with a destination pRNA vector containing a GFP CDS at either the N- or C-terminus using Gateway technology (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Glutathione determinations were normalized to the protein content of the insoluble pellet from the SSA extracts 2.5% SDS in 0.1 N bicarbonate using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL).
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bioRxiv - Neuroscience 2020Quote: ... and 1 µM TMRM (tetramethylrhodamine, methyl ester, perchlorate; Invitrogen), and incubated at 37°C for 15 min ...
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bioRxiv - Bioengineering 2020Quote: ... methyl ester (TMRM) (0.1µM in hepatocyte medium, Thermo Fisher) to visualize active mitochondria ...
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bioRxiv - Bioengineering 2022Quote: ... mitochondrial membrane potential (tetramethylrhodamine methyl ester, TMRM; ThermoFisher Scientific) and mitochondrial superoxide (MitoSOX Red ...
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bioRxiv - Genomics 2022Quote: ... Histone H3 Lysine 4 tri-methyl (ThermoFisher PA5-27029), Histone H3 Lysine 27 acetylation (Abcam ab4729) ...
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bioRxiv - Immunology 2022Quote: ... 12.5 mM methyl-α-d-mannopyranoside (Thermo Fisher Scientific), 100 U/ml penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were stained with tetramethylrhodamine methyl ester (TMRM) (Invitrogen) at a non-quenching concentration (20nM) ...
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bioRxiv - Cancer Biology 2022Quote: ... methylparaben (Methyl 4-hydroxybenzoate, MP; Acros Organics, CAT: 126961000), and butylparaben (Butyl 4-hydroxybenzoate ...
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bioRxiv - Genetics 2023Quote: ... and Tetramethylrhodamine methyl ester (TMRM, 0.1µM, Thermo Fisher I34361) for 30 minutes at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... and tetramethylrhodamine methyl ester perchlorate (TMRE) (ThermoFisher, Waltham, MA) dyes ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... composed of aliquots of each EV sample from the discovery cohort (n=22 individuals) was desalted with Pierce desalting spin columns (Thermo Fisher Scientific) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection was carried out in antibiotic-free culturing media (6ml) using 10μl lipofectamine (Lipofectamine ® 2000Reagent, 1mg/ml, Invitrogen P/N 2887) in 400μl Opti-mem media (Gibco Opti-mem reduced serum media ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells stably expressing a single copy of N-terminally tagged TTBK2- or GFP-BirA* were generated using Flp-IN T-REx cells (ThermoFisher K6500-01), Positive clones were selected with hygromycin resistance ...
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bioRxiv - Cell Biology 2020Quote: ... labelled with the fluorescent lipid dye BODIPY® FL Maleimide [BODIPY® FL N-(2-Aminoethyl) Maleimide] (Thermo Fisher Scientific; 1:200) and incubated at room temperature in the dark 10 minutes prior to analysis ...
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bioRxiv - Immunology 2021Quote: ... to target the N1 amplicon of the N gene along with TaqPath 1-Step RT-qPCR Master Mix (Applied Biosystems Waltham, MA). For the subgenomic assay ...
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bioRxiv - Genetics 2021Quote: The coding sequence for the pH insensitive Citrine fluorescent protein was inserted in the coding sequence of the BK2L3 protein (Zm00001d034049) immediately after the N-terminal secretory peptide domain and synthesized in the pDONOR221 vector (Life Technologies, USA) (Tian et al. ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... GEMs were broken and the single-strand cDNA was cleaned up with DynaBeads MyOne Silane Beads (Thermo Fisher Scientific; P/N 37002D). cDNA was amplified using the C1000 Touch Thermal cycler with 96-DeepWell Reaction Module ...
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bioRxiv - Neuroscience 2021Quote: ... and for snRNA-seq the final resuspension occurred in N-PBS (PBS, 5% BSA, 5μg/mL actinomycinD and 0.2U/μl RNAse inhibitor (Thermo Fisher Scientific, Cat#N8080119)).
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bioRxiv - Neuroscience 2021Quote: ... Day 14 control and CLN5i neurons were used to isolate total cell lysates using N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific, 87792), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... liquid chromatography was performed with nanoUHPLC Easy nLC 1000 (Thermo Scientific brand, model EASY-nLC 1000, Easy-Spray ColumnPepMap RSLC, P/N ES801) (Thermo Scientific) coupled to a mass spectrometer with Orbitrap technology ...
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bioRxiv - Molecular Biology 2021Quote: ... The HAP1 wild type and CRISPR/Cas9 edited human knockout cell lines obtained from Horizon Genomics GmbH (Vienna, Austria) were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) (Invitrogen, cat n°31980-048). More specifically ...
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bioRxiv - Biochemistry 2020Quote: Global proteomics for all comparative method testing was carried out (n=3 per group) on an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) coupled to an Eksigent nanoLC-2D system through a nanoelectrospray LC − MS interface ...