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Citations for Thermo Fisher :
1551 - 1600 of 6146 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: Rats (N = 4) were infused unilaterally with AlexaFluor-594 conjugated cholera toxin B (CTb; 5 μg/μL; Life Technologies, San Diego, CA) into the mOFC (AP +4.1 ...
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bioRxiv - Cell Biology 2019Quote: ... GM2610 and AICS-0023 clone 20) were maintained under feeder-free condition using Essential 8 (E8) medium and vitronectin (VTN-N) (Thermo Fisher Scientific). In some cloning experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The N gene plus the upstream T7 promoter region was PCR-amplified from pSG220 using Phusion™ High-Fidelity DNA polymerase (Thermo Scientific) and standard primers ACYCDuetUP1 and DuetDOWN1 ...
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bioRxiv - Systems Biology 2021Quote: ... The UHPLC was equipped with a trap column (Acclaim PepMap 100, 75 μm x 2 cm, C18, P/N 164535, Thermo Fisher Scientific) and a 50 cm analytical column (Acclaim PepMap 100 ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA; anti E-Cadherin antibody: CD324 #16-3249-82, Invitrogen, USA) to bind to their respective epitopes ...
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bioRxiv - Molecular Biology 2021Quote: ... Genes were cloned into modified pRSF1-Duet expression vector with a TEV-cleavable N-terminal 14-histidine tag (39) using New England Biolabs and Anza (Thermo Fisher Scientific) enzymes ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:1000 in PBS for 15 m at RT and mounted on glass microscope slides (Fisher Scientific, cat n. 1157-2203) using VectaShield Vibrant mounting reagent (2B Scientific).
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bioRxiv - Immunology 2020Quote: ... blood was collected postmortem via heart puncture on day two after infection (n = 3 mice/treatment) and kept at −20 °C in RNAlater (Thermo Fisher Scientific). RNA was extracted using the Mouse RiboPure Blood RNA Isolation Kit and globin RNA depleted using the GLOBINclear Kit ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Tumor samples with sufficient high-quality RNA (n=5) were analyzed on the Affymetrix GeneChip Human Genome U133 Plus (v.2.0) Array (Affymetrix, Santa Clara, USA) at the Microarray Department of the University of Amsterdam ...
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bioRxiv - Cell Biology 2021Quote: ... co-cultures were fixed in 4% PFA for 30 min and immunostained for the anti-N (protocol described above) and with HCS Cell Mask TM Blue Stain (Invitrogen, 1:300). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
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bioRxiv - Developmental Biology 2022Quote: ... or 6 dpf zebrafish from one linc-mipep-/- heterozygous incross (for single-cell Multiome, n=12 per sample) in ice-cold Neurobasal media supplemented with B-27 (Thermo Fisher Scientific), snap-frozen in a dry ice/methylbutane bath (to preserve nuclear structure) ...
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bioRxiv - Biochemistry 2022Quote: N-terminal amino acid sequencing for the eOD-GT8 60mer was performed using the Procise Protein Sequencing System (Applied Biosystems, CA, USA). The protein band corresponding to the eOD-GT8 60mer (~50 kDa ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and overlaid with 200 μL of alkylation buffer (40 mM HEPES, 50 mM NaCl, 1 mM EGTA, protease inhibitors) supplemented with 25 mM N-ethylmaleimide (NEM; Fisher Scientific, AA4052606). Cells were incubated for 10 minutes at room temperature and then 20 μL of 10% CHAPS detergent (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Banked adipose-derived stem cells (ADSCs) were purchased from CellTex Therapeutics (CellTex, catalog # N/A) and cultured in growth media containing DMEM-F:12 (Gibco, catalog # 11320033) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Global proteomics for all comparative method testing was carried out (n=3 per group) on a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Subsamples of 1000–2000 μg were carefully weighed in tin capsules to determine the total N percentage (N% as mg (100 mg DW)−1) and the 15N abundance using a FLASH 2000 Organic Elemental Analyzer (Thermo Fisher Scientific) coupled to a Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... One-step real-time RT-PCR assays targeting SARS-CoV-2 nucleocapsid (N) coding region and subgenomic RNA were run on ABI 7900HT Sequence Detection Systems (Thermo Fisher Scientific), as previously reported25.
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses of purified and desalted peptides were performed on a Dionex UltiMate 3000 n-RSLC system (Thermo Fisher Scientific), connected to an Orbitrap FusionTM TribridTM mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: COCs were collected and analyzed after 24 h of IVM by means of ROS content (n=60; minimum of 20 per group) with CellROX® Green Reagent (CRG) (Thermo Fisher) and MMP (n=60 ...
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bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Biophysics 2023Quote: Protein solutions (lysozyme, thyroglobulin, HSA, RNase A, HLA, and α-synuclein) were incubated with N-hydroxysuccinimide (NHS)-ester-functionalized Alexa 488 (Thermo Fisher) at 5-molar excess of dye and incubated at room temperature for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) at 150V for 2h using NuPAGE 2-(N-morpholino)ethanesulfonic acid SDS running buffer (NP0002, Thermo Fisher Scientific), NuPAGE Antioxidant (NP0005 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the total protein concentration was determined using a Pierce Reducing Agent Compatible BCA kit (RAC-BCA, Thermo Scientific, P/N 23252) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length genes of human RNGTT and CMTR1 were cloned into pMlink vector containing a 4×ProteinA tag at the N-terminal and were transfected to Expi293F cells (Thermo Fisher Scientific) when cell density is 2.5× 106 /ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified MuSCs from each replicate were stained for viability with calcein-AM and ethidium-homodimer1 (P/ N L3224 Thermo Fisher Scientific). scRNA libraries were generated using the GemCode Single-Cell Instrument (10x Genomics ...
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bioRxiv - Cancer Biology 2023Quote: Ewing sarcoma A673 (ATCC CRL-1598) and neuroblastoma SK-N-AS (ATCC CRL-2137) cell lines were grown in DMEM (Gibco, #31966-021) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Cancer Biology 2023Quote: ... The Flag plasmids were transiently transfected in to N/Tert-1 cells using Lipofectamine™ RNAiMAX transfection (Invitrogen, catalog no. 13778-100) protocol and transfected in to U2OS cells using the calcium phosphate transfection protocol.
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bioRxiv - Developmental Biology 2023Quote: ... both diluted 1:50 in DMEM/F-12 or with 20 ug/mL recombinant truncated vitronectin (VTN-N) (Thermo Fischer Scientific, A31804) diluted in DMEM/F-12 (Gibco, 21331-020) for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from MG63 lysates (n = 2 per treatment condition) using the PureLink RNA Mini kit (12183018A, Thermo Fisher Scientific) with DNase set (12185010 ...
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bioRxiv - Cell Biology 2023Quote: Liquid chromatography separation was performed at a flow rate of 300 nL/mL on a C18 pre-column (Acclaim PepMapTM 100, 75 μm×2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMapTM RSLC C18 ...
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bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
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bioRxiv - Genetics 2023Quote: ... The optimized and minimum required protocol for organoid hepatic isolation consisted of rinsing the fresh tissue in Phosphate Buffered Saline (PBS)/N-acetylcysteine (NAC) once and then transferring it to a tube filled with Advanced DMEM/F12 (Gibco; 12634-010), mincing the tissue into small fragments ...
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bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... at a density of 107 cells in 19 ml of medium for dual-proteomics or at 1.5 x 104 cells per well in 96-well plates (Nunc, cat n° 164590) in 0.2 ml of medium for MTT ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Immunology 2022Quote: ... which was created via the in vitro T7-DNA-dependent RNA transcription of a linearized DNA molecule containing the target region of the N gene full-length by using MEGAscript T7 Transcription Kit (ThermoFisher, Cat# AM1334), and purified with MEGAclear Transcription Clean-Up Kit (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... The roots were lyophilized in vacuo and analyzed for total N and 15N content using elemental analysis–isotope ratio mass spectrometry (Flash EA1112-DELTA V PLUS ConFlo III system, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Infectious titers were determined by focus-forming unit assay whereby foci of infection were measured using anti-nucleocapsid (N) antibody (Invitrogen; MA1-7403). Where indicated ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: A total of 12 WT and pink1 KO mice (N = 3 per sex/genotype) at age of three months were anesthetized and intracardially perfused with cold PBS (Invitrogen, 14190-235). Brains were removed and immediately flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and granulocyte-macrophage colony-stimulating factor (GM-CSF, 10 ng/ml) to DMEM/F12 (HyClone™, SH30023.01) containing N-2 supplement (Gibco™, 17502048). This protocol facilitated the maturation process of the PMPs into microglia.
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bioRxiv - Molecular Biology 2024Quote: ... level was measured from the plasma of representative subsets of the groups (n=16) using an automated analyzer (Indiko Plus, Thermo Fisher, US).
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Biochemistry 2023Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...