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Citations for Thermo Fisher :
1651 - 1700 of 6146 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 1 ng/mL (n = 3) and 10 ng/mL (n = 3) treatment were subjected to albumin depletion according to the manufacturer’s instructions (Thermo Fisher Scientific, Loughborough, UK). Individual samples were digested with trypsin (2.5µg trypsin ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were either Nissl-stained with cresyl violet or stained with the fluorescent Nissl reagent NeuroTrace Blue (1:200) (Life Technologies, N-21479). In four subjects ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was incubated 2h at 37°C and then loaded on a 15% acrylamide/8M urea precast gel (Invitrogen, cat n° EC6885BOX). The ligated RNA was purified through gel extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... The second strand cDNA was generated by performing one PCR cycle using Platinum II Hot start Taq Polymerase (Thermo Fisher, cat n°14966001). The reaction included 20 μLsingle-strand cDNA ...
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bioRxiv - Microbiology 2021Quote: ... PM-WCPV-S) or N peptide pool (Meltenyi, 130-126-698) in the presence of 1 µg/mL anti-CD28 (Invitrogen, 14-0281-86) for co-stimulation for 6 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... The quantity and quality of the isolated RNA (n = 3, independent pools of fascicles from different mice) was determined with a Qubit® (1.0) Fluorometer (Life Technologies, California, USA) and a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA was generated using 500 ng of TotalRNA and Applied Biosystems High Capacity cDNA with Reverse Transcription kit (Applied Biosystems p/n 4368814). cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... NiV RdRp P/L complex was purified using Ni-NTA affinity chromatography based on the P protein N-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). The protein identities of the purified HCV nsp5b RdRp and NiV RdRp P/L complex were confirmed by mass spectrometry (MS ...
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bioRxiv - Microbiology 2021Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS-CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described [41] using the following primers and probes ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were first loaded onto an Acclaim™ PepMap™ 100 C18 (3 μm, 75 μm ID× 20 mm length, P/N 164946 Thermo Scientific), and then separated on C18 packed emitter column (3 μm ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then cross-linked at 120J/m2 and then labelled RNA was detected using the Chemiluminescent Nucleic Acid Detection module kit (Thermofisher, cat n°: 89880) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Chemical probes used in this work include alkDADA (Liechti et al., 2014), N-AlkTMM and O-AlkTMM (Foley et al., 2016) and FM4-64 FX (Invitrogen, Carlsbad, CA USA). AlkDADA was synthesized by the Einstein Chemical Biology Core and the TMM probes were kind gifts of Dr ...
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bioRxiv - Neuroscience 2022Quote: ... analyses cultured NSPCs or hippocampal tissue (at least n=3 per condition) were collected at indicated time-points for each experiment with RIPA buffer (Cat# A32963, Thermo Fisher, Waltham, USA) plus a protease inhibitor cocktail with the addition of phosphatase inhibitors (Cat#78441 ...
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bioRxiv - Biophysics 2022Quote: ... The terminal free amine on the spacer was reacted with the N-hydroxysuccinimide (NHS) ester group of sulfo-SMCC (Thermo Fisher Scientific, 22622). The maleimide group of sulfo-SMCC was subsequently conjugated to 3’ thiol modified DNA oligonucleotides as described19 ...
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bioRxiv - Immunology 2023Quote: Open-reading frames coding for either Nefmut alone or fused with SARS-CoV-2 N protein were cloned into pVAX1 plasmid (Thermo Fisher, Waltham, MA) as previously described [20] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: The RBD protein was incubated for 30 min at 21 °C and 750 rpm with a 10 molar equivalent of sulfo-m-maleimidobenzoyl-N-hydoxysuccinimide ester (Sulfo-MBS) (Thermo Scientific; MW 416.30). The excess crosslinker was removed by passing the protein through a PD-10 column using a PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:30 rat anti-N-cadherin (Developmental Studies Hybridoma Bank, DN-EX #8,) and 1:200 rabbit anti-lucifer yellow (Molecular Probes, A-5750). The secondary antibodies used were 1:500 goat anti-rabbit with Alexa fluor 633 (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS- CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described (73 ...
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bioRxiv - Biochemistry 2023Quote: ... n=4) collected in microcentrifuge tubes were subjected to a 30-min incubation in 30 μM iodoacetamide (IAM) (Thermo Fisher Scientific, Waltham, MA) or N-ethylmaleimide (NEM ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: MARC-145 cells transfected with the recombinant pCAGGS-N plasmid were lysed in Pierce™ IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA), and then mixed with each of the purified anti-N mAbs ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 568 conjugated secondary antibodies (Invitrogen; 1:1000 in blocking buffer). Actin tails were labeled with Alexa Fluor 488 phalloidin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The peptides were separated at a flow rate of 300 nL/min on C18 pre-column (Acclaim PepMap™ 100, 75 µm × 2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMap™ RSLC C18 ...
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bioRxiv - Genomics 2023Quote: ... Cells were then plated onto Geltrex-coated surfaces at 1.5×105 cells/cm2 in the induction medium (DMEM/F12 supplemented with 1× N-2 (Thermo Fisher Scientific, cat# 17502048), 1× non-essential amino acids (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The N concentrations were determined by dry combustion of a 3-4 mg sample with a Flash EA1112 elemental analyzer (Thermo Scientific, Rodano, Italy).
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bioRxiv - Biochemistry 2022Quote: ... The peptides were separated at a flow rate of 300 nL/minute on C18 pre-column (Acclaim PepMap™ 100, 75 μm□×□2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMap™ RSLC C18 ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from the LV myocardium of 2-month-old male WT and Rbm20ΔRS mice (n = 3 per group) using TRIzol Reagent (Life Technologies, Waltham, MA, USA) and treated with DNase I (cat# D9905K ...
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bioRxiv - Plant Biology 2023Quote: ... Equal amount of protein samples were electrophoresed on 10 % NuPAGE® Bis-Tris Gels in 1X MES [2-(N-morpholino) ethane sulfonic acid] SDS buffer (Invitrogen, Waltham, MA) and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: Total protein from muscle tissue samples (n = 6 for EarlyMT and n = 16 for LateMT) was extracted using an extraction buffer containing Tissue extraction Reagent I (FNN0071, Invitrogen, Waltham, Massachusetts, USA), HALT Protease and Phosphatase Inhibitor Cocktail (1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
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bioRxiv - Neuroscience 2023Quote: ... and maintained in N2B27 media (1:1 of DMEM F12 Glutamax [Thermo Fisher Scientific, 10565018] and Neurobasal media [Thermo Fisher Scientific, 12348017], 1X N-2 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
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bioRxiv - Neuroscience 2024Quote: ... Neuronal differentiation was induced by culturing NGN2-NPC in neural differentiation medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco®) supplemented with doxycycline (2µg/ml) ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCRs were performed in technical and independent experiment triplicates (n=3) with Power Sybr Green Master Mix (Life Technologies, Darmstadt, Germany) on a VIIA7 (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR reactions were run in triplicates (n=3) in the 7500 Real-time PCR system by Applied Biosystems (Thermo Fisher Scientific) using power SYBR Green PCR master mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... or 5 × 103 cells/mL (N. gruberi) in 100 µL of the indicated medium in white TC-treated 96-well plates (Thermo Scientific Nunc Microwell 136101). For assays under standard growth conditions (“+ glc”) ...
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bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
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bioRxiv - Bioengineering 2020Quote: Before staining with either 5-bromo-4-chloro-3’-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, ThermoFisher) or Alizarin Red S (ARS ...
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Cell Biology 2021Quote: ... pH 7.5) supplemented with 2.4mg/mL nicotinamide and a combined phosphatase and protease inhibitor cocktail (Thermo Fisher) or cardiac mitochondria prepared as described above ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then labelled for Methoxy-X04 (10 minutes at RT) or counterstained with DAPI and mounted with prolong gold anti-fade medium (Invitrogen, P36930). Images were acquired using a Zeiss LSM700 confocal microscope with the Zen 2010 Software package ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Cell Biology 2019Quote: ... First antibody incubation was done O/N at 4°C and the secondary HRP-coupled antibodies goat anti-Mouse IgG (H+L) (ThermoFisher scientific; 31430; 1:10,000) or polyclonal HRP-conjugated goat-anti-rabbit IgG (ThermoFisher scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to a Q Exactive hybrid mass spectrometer (Thermo) (N-terminal FLAG-tagged datasets) or an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer (C-terminal FLAG-tagged datasets) ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Physiology 2022Quote: Samples of frozen mouse liver were homogenized in lysis and extraction buffer (RIPA lysis and extraction buffer, catalog n° 89900, Thermofisher Scientific, Waltham, MA, USA) containing protease inhibitors (protease inhibitor cocktails tablets ...
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bioRxiv - Physiology 2022Quote: ... equal amounts of the protein samples (20 μg of liver extracts) were resuspended in Laemmli sample buffer (catalog n°39000, Thermofisher Scientific, Waltham, MA, USA) and separated in an 4%–20% sodium dodecylsulfate (SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... the inserts of the entry vectors were recombined into the into the pDEST 5’ BirA*-FLAG-pcDNA5-FRT-TO destination vector (Invitrogen, cat n°12285-011) using LR-clonase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... [30] at a concentration of 57.5 μM (5% of the suggested concentration in RPMI 1640 medium) and media were supplemented with 10% dialyzed fetal bovine serum (Invitrogen, cat n°26400-044). Cell populations were cultured for at least six population doublings for complete incorporation of the labelled L-Arg.