Labshake search
Citations for Thermo Fisher :
1501 - 1550 of 6146 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... EJC mutant and wild-type sibling embryos (N = 25) were harvested at 21 and/or 27 hpf and lysed in 500 µl Trizol (Thermo Fisher Scientific). RNA was extracted following manufacturer standard procedures.
-
bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
-
bioRxiv - Developmental Biology 2019Quote: ... Total RNA was extracted from pooled embryos from heterozygote intercrosses or AB strain WT (n=20-30) using Trizol (Life Technologies, 15596). cDNA was prepared with SuperScript III/IV Reverse Transcription Kit (Life Technologies ...
-
bioRxiv - Genetics 2020Quote: ... Total RNA was extracted from the left side of thorax with gynomorph-like color where siRNA was introduced and from the control region on the right side of the thorax of the same individual (N = 4 each for male and andromorphic female adults) using RNAqueous™-Micro Total RNA Isolation Kit (Thermo Fisher). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Thermo Fisher ...
-
bioRxiv - Developmental Biology 2019Quote: ... After tissue attachment, Dulbecco’s modified Eagle’s medium (Biological industries, cat: 01-053-1A) supplemented with N-2 Supplement (100X) (Thermo Fisher Scientific, cat: 17502001) and 1% FBS (Biological industries ...
-
bioRxiv - Neuroscience 2020Quote: ... and then 12 µg from each was injected into a 75 μm × 2 cm nanoViper C18 Acclaim PepMap100 precolumn (3 μm particle size, 100 Å pore size; P/N 164705; Thermo Scientific). Peptides were separated at a flow rate of 250 nl/min and eluted from the column over a 5 hours gradient starting with 0.1% FA in ACN (5-30% ACN from 0 to 280min followed by 10min ramping up to 80% ACN ...
-
bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
-
bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
-
bioRxiv - Biochemistry 2020Quote: pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... U2OS cells were first transfected with PiggyBac Tet-On 3G-(ArrayG/N)16X and PBQM-mwGFP plasmids using the Neon Transfection system (Thermo Fisher Scientific) and the following pulse program (pulse voltage ...
-
bioRxiv - Neuroscience 2020Quote: Hippocampi removed from euthanized 8-month-old 5×FAD mice previously bilaterally injected with saline or N-Aβcore were homogenized with 200-250 μL of Pierce I.P Lysis Buffer (ThermoFisher Scientific, # 87788, lot# MJ162614) with 1x Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 488 conjugated secondary antibodies (Invitrogen; 1:1000 in PBS). Actin tails were labeled with Alexa Fluor 488 ...
-
bioRxiv - Biochemistry 2021Quote: Formerly N-glycopeptides were separated by nano-LC-MS/MS coupled to an ESI-LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). Mass spectrometric analysis was performed with the following settings ...
-
bioRxiv - Microbiology 2021Quote: ... sterols dissolved in chloroform were dried under nitrogen gas (N-EVAP, Organomation) followed by high vacuum for at least 1 hour (Savant SpeedVac, Thermo Fisher Scientific). They were then hydrated with 2.5 mM MβCD prepared in serum-free medium ...
-
bioRxiv - Microbiology 2021Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
-
bioRxiv - Immunology 2021Quote: ... lungs and kidneys were isolated from perfused animals and incubated over-night (O/N) in RNAlater™ stabilisation solution (Thermo Fisher Scientific) for RNA analysis ...
-
bioRxiv - Plant Biology 2021Quote: ... Final constructs were obtained by transferring all candidate genes subsequently into the plant binary destination vector pAlligator2 (N-terminal triple HA fusions=HA3) (Bensmihen et al., 2004) via LR reactions (Thermo Fisher Scientific). Constructs were transformed into Agrobacteria (Rhizobium radiobacter ...
-
bioRxiv - Molecular Biology 2020Quote: ... CK2A1 or PRKACA coding sequences were cloned into the destination vector pDEST-pcDNA5-BirA*-FLAG N-term with LR clonase 2 (Thermo Fisher Scientific) using the Gateway system ...
-
bioRxiv - Cell Biology 2020Quote: ... was solubilized with 0.1 N NaOH and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA). Cellular cholesterol content data was expressed as mg cholesterol/mg of protein ...
-
bioRxiv - Biophysics 2021Quote: ... was cloned into a modified pEZT-BM vector with an N-terminal Flag tag (Morales-Perez et al., 2016) and heterologously expressed in HEK293F cells using the BacMam system (Thermo Fisher Scientific). Bacmids were synthesized using E ...
-
bioRxiv - Molecular Biology 2022Quote: ... If required (as indicated for the methods for the respective experiments) hiPSCs were adapted to feeder-free conditions on Vitronectin (VTN-N, Thermo Fisher Scientific) coated plates in mTeSR-plus (StemCell Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... the pellet (synaptosomal fraction) was re-suspended and sonicated in N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific, Waltham, MA, USA; 87792). Protein concentration was determined via the BCA protein assay kit (ThermoFisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
-
bioRxiv - Biophysics 2022Quote: ... and apoA1 were purified using established protocols for MSP44 with the following modifications: A synthetic construct of apoA1 bearing a 3C protease cleavable N-terminal deca-histidine tag (GeneArt/Thermo Fisher Scientific) was cloned into a pET28a vector (Addgene ...
-
bioRxiv - Microbiology 2022Quote: ... a plasmid encoding the EGFP-N fusion protein was constructed and transfected into infected HEp-2 cells with Lipo3000 (Thermo Fisher, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... a pET21a vector including hTS with an N-terminal 6x histidine tag was transformed into BL21 (DE3) cells lacking the rne131 gene (Invitrogen, Carlsbad, CA). After an adaptation period (Bonin & Lee ...
-
bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
-
bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
-
bioRxiv - Microbiology 2020Quote: ... we introduced a stop codon in the pLRB302 plasmid at residue 640 of the envelope ORF (C640Stop) located at the N’ terminal of the cytosolic tail using the Phusion Site-Directed Mutagenesis Kit (Thermo Fisher Scientific) and the following primers ...
-
bioRxiv - Immunology 2020Quote: ... FcMBL was biotinylated at the N terminus of the Fc protein using an N-terminal amino-oxy reaction and then coupled to streptavidin superparamagnetic beads (1µM MyOne Dynabead (Thermo Fisher Scientific, USA). Samples were screened using 5 µg of the FcMBL beads ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RBD WT for SPR binding assays (with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
-
bioRxiv - Plant Biology 2021Quote: ... Immunoblotting of MSD2-mCherry was performed after native-PAGE or SDS-PAGE (12.5% separating gel and 4% stacking gel) with anti-mCherry primary antibody (Invitrogen, cat. n. M11217) diluted to 1:5,000 in Tris-buffered saline with 0.1% (v/v ...
-
bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... Tryptic peptides (2 μg) were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 ul/min of solvent A (0.1% FA and 3% acetonitrile in LC-MS grade water) ...
-
bioRxiv - Microbiology 2020Quote: ... at a flow rate of 0.3 μL/min and separated on a C18 analytical column (75 um x 50 cm; PepMap RSLC C18; P/N ES803; Thermo Fisher Scientific).
-
bioRxiv - Plant Biology 2021Quote: The pCR8-ISR1 construct was used to subclone the gene into the pMDC32 (35S cauliflower mosaic virus [CaMV] promoter) or pEarleyGate103 (35S CaMV promoter with an N terminal GFP fusion) vectors using Gateway LR Clonase II (Thermo Fisher Scientific) and then stably expressed into isr1-1 and isr1-2 plants following Agrobacterium tumefaciens (strain GV301)-mediated transformation using the floral dip method (Clough and Bent ...
-
bioRxiv - Neuroscience 2021Quote: ... The barcode sequencing libraries were quantified using a Qubit 2.0 with a Qubit™ dsDNA HS Assay Kit (Invitrogen P/N Q32854) and the quality of the libraries were performed on a 2100 Bioanalyzer from Agilent using an Agilent High Sensitivity DNA kit (Agilent P/N 5067-4626) ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40 supplemented with 50,100 or 200 mM KCl) and proteins were eluted by incubation in 1x LDS-sample buffer (Thermofisher, c/n NP0007) at 65°C for 10 minutes ...
-
bioRxiv - Microbiology 2022Quote: SpRecAA488 was made by covalently modifying primary amines (lysines or N-ter) of the protein with Alexa 488-succinidimyl ester (Molecular Probes, ThermoFisher), in presence of an excess of ssDNA (M13 mp18 ...
-
bioRxiv - Microbiology 2022Quote: APEX2 coding sequence (56) with the FLAG epitope at the N-terminus optimized for expression in human cells was synthesized by Invitrogen (GeneArt service). For the transient expression ...
-
bioRxiv - Neuroscience 2022Quote: ... iAstrocyte differentiation was initiated by switching the medium to astrocyte medium (DMEM/F12+GlutaMAX, 1% N-2 supplement, 1% fetal bovine serum, all from Thermo Fisher Scientific) as previously described [81] and continued for at least 60 days ...
-
bioRxiv - Cell Biology 2022Quote: ... transgenes were recombined with the Drosophila Gateway Vector Collection (DGVC) destination vectors expressing the N terminal tag fused target proteins under Actin5C promoter (Invitrogen, Carlsbad, CA). In addition ...
-
bioRxiv - Cell Biology 2019Quote: The pellets (n = 5/time point/group) were incubation at 48-well plate in serum-free DMEM medium 200 μl alamarBlue (Invitrogen, California, USA) 20 μl ...
-
bioRxiv - Genomics 2019Quote: ... and wild type (n = 9) mice were determined using a Countess Automated Cell Counter according to manufacturer’s protocol (Life Technologies, Carlsbad, CA). For the Tug1rescue experiment ...
-
bioRxiv - Molecular Biology 2020Quote: ... linker oligos with a 5’-terminal amine modification were obtained from IDT (Coralville, Iowa) and activated using sulfo-SMCC (Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) reagent (Thermo Fisher, #A39268) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... surface lysines on MS2 capsids were modified with fluorescent dyes (NHS-Atto488 or NHS-Atto633) and the heterobifunctional maleimide-PEG24-N-hydroxysuccinimide ester crosslinker (Thermo Fisher Scientific). Second ...
-
bioRxiv - Systems Biology 2019Quote: ... with 5’-amino modification and were coupled with NHS (N-hydroxysuccinimide)-ester labelled fluorescent dyes (Alexa 488, 594, 647 (Thermo Fisher Scientific) and Cy3B (GE Healthcare) ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the Sensation Plus FFPE Amplification and WT Labeling Kit (P/N 703089, Rev.4 Thermo Fisher Scientific Inc., Life Technologies). GeneChip ST Arrays (GeneChip Human Gene 2.1 ST Array Plate ...
-
bioRxiv - Bioengineering 2020Quote: ... 30 and 60 min after induction of neutralization of Col 1 and enzymatic gelation (n=2-5 samples/group) of freshly prepared materials with a Multiskan™ GO Microplate Spectrometer (Thermo Scientific). The following solutions were analyzed ...
-
bioRxiv - Bioengineering 2021Quote: ... The primate ovaries were dissected into approximately 60 fragments measuring 1×1×1mm (1 mm3) and transferred into maintenance media (α- MEM, P/N 32561, Gibco, USA) prior to encapsulation (Figure 4) ...