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Citations for Thermo Fisher :
1701 - 1750 of 6146 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Catalog No. 22980) and Sulfo-NHS (N-hydroxysulfosuccinimide, Catalog No. 24510) were purchased from Thermo Scientific (Waltham, MA, US). Phosphate-Buffered Saline (PBS ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies, Darmstadt, Germany). Fold expression changes were determined using a standard 2−ΔΔCT method that compares CT (cycle threshold ...
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bioRxiv - Developmental Biology 2019Quote: ... C- and N-terminal GFP-tagged Nubp1 and Nubp2 expression plasmids [32] were transfected using Lipofectamine® 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Nikon C2 Confocal microscope.
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bioRxiv - Physiology 2019Quote: ... Purified RNA (100 ng per sample) was labeled with the Whole-Transcript Sense Target Assay kit (Affymetrix, Santa Clara, CA, USA; P/N 900652) and hybridized to an Affymetrix Mouse Gene 1.0 arrays or 2.1 ST array plate (Affymetrix) ...
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bioRxiv - Neuroscience 2019Quote: ... cycling synaptic vesicles were stained with the styryl dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino) styryl) pyridium dibromide (FM1-43; Molecular Probes, Inc., Eugene, OR). For labeling of synaptic vesicles ...
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bioRxiv - Biophysics 2021Quote: ... The silanized surfaces were incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2022Quote: The reverse transcription was performed with 500 ng of total RNA using the High-capacity cDNA reverse transcription kit (ThermoFisher Scientific, catalog n°4368814) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... A nanoViper Trap Column (C18, 3 μm particle size, 100 Å pore size, 20 mm × 75 μm ID; Thermo Fisher Scientific, P/N 164946) was used for desalting ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were run on a PepMap RSLC C18 column (2 μm particle size, 15 cm × 50 μm ID; Thermo Fisher Scientific, P/N ES901). A nanoViper Trap Column (C18 ...
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bioRxiv - Biophysics 2022Quote: ... was spiked with 1% of Alexa 488-labeled HU-B mutant (containing an N-terminal Cys conjugated with Alexa Fluor™ 488 C5 Maleimide from Thermofisher; Cat no. A10254). The 488 nm laser was set at 100% for efficient bleaching and focused on a small circular region with a diameter of 1 μm ...
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bioRxiv - Developmental Biology 2019Quote: ... The silicon device surfaces were functionalized with a previously described protocol using 3-mercaptopropyltrimethoxysilane (MPTMS) and N-gamma-maleimidobutyryloxysuccinimide ester (GMBS) NeutrAvidin-biotin chemistry (Thermo Fisher Scientific, Rockford, IL)[32 ...
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bioRxiv - Developmental Biology 2020Quote: The retinas of juveniles (n = 6 retinas from 5 animals) and adults (n = 5 retinas from 4 animals) were dissected out and put in RNAlater (Ambion Inc., Austin, TX, USA). RNA extraction was performed using the RNeasy mini kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Genetics 2020Quote: Guinea pig anti-zebrafish Rad21l1 polyclonal antibody production: An N-terminal fragment of Rad21l1 cDNA was amplified with Phusion DNA polymerase (Thermo Fisher Scientific, Catalog #: M0530L) using the following primers ...
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bioRxiv - Plant Biology 2021Quote: ... C and N concentrations (mass based) of the samples (5 mg dry weight) were determined with Elemental analyzer (Flash EA 2000, Thermo Fisher Scientific, Bremen, Germany) in four biological replicates ...
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bioRxiv - Cell Biology 2020Quote: ... n-dodecyl-β-D-maltoside and assessed for OxPhos complex content using a Native PAGE Novex Bis-Tris Gel system (Invitrogen, Carlsbad, CA, USA). The separated proteins were transferred to polyvinylidene fluoride membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... for amplification of 100ng of cDNA template from N/D/S cells either treated with MS351 or vehicle using Power SYBR Green Master Mix (Thermo Fisher Scientific Cat. 4368577) qRT PCR protocol14 ...
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bioRxiv - Cancer Biology 2022Quote: ... with or without N-ethyl-N-nitrourea 10μg/ml or 50μg/ml and treated 2h with 1mg/ml Collagenase type IV (Gibco by life technologies ref 17104-019).
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bioRxiv - Neuroscience 2023Quote: ... for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse ...
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... Cell culture supernatants containing specific anti-N mAb were harvested and concentrated using Pierce™ Saturated Ammonium Sulfate Solution (Thermo Fisher Scientific, Waltham, MA). Biotinylation of the mAb was performed using a Biotin Conjugation Kit by following the manufacturer’s instruction (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Bulk RNA was isolated from TA muscles of five mice from each of the four treatment groups (n=20) using Trizol (ThermoFisher Scientific, Waltham, MA, USA) and established methods.32 A NanoDrop spectrophotometer was used to determine RNA purity ...
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bioRxiv - Biophysics 2023Quote: Human neuroblastoma cell line (SK-N-BE) stored at -80°C in freezing medium (RPMI Gibco® supplemented with 20% FBS Gibco and 10% DMSO), were quickly defrosted at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Sequence-verified Cas9-gRNA-Puro plasmids and pMA donor plasmid were co-transfected into in-house C57BL6/N 6.0 ESC using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) prior to selection with 2 μg/ml puromycin for 48 hours ...
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bioRxiv - Biochemistry 2023Quote: ... as three full experimental technical replicates (n=54) with ∼100 ng of peptides injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... 5,000 μg of dry powder was placed in tin capsules to quantify the total N and C concentrations using the FLASH 2000 Organic Elemental Analyzer (Thermo Fisher Scientific Villebon, France) and the 15N enrichment using the Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole-cell lysates were prepared from 5x106 cells treated with N-acetylcysteine (MilliporeSigma A7250) for 4 hours followed by stimulation/oxidation with 1 mM hydrogen peroxide (Fisher Scientific Canada, Mississauga ON) for 5 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
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bioRxiv - Biochemistry 2024Quote: ... or 5 × 103 cells/mL (N. gruberi) in 100 µL of the indicated medium in white TC-treated 96-well plates (Thermo Scientific Nunc Microwell 136101). For assays under standard growth conditions (“+ glc”) ...
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bioRxiv - Biophysics 2021Quote: ... Methyl-PEG4-thiol (MT(PEG)4) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 2 mM 3-methyl-2-oxobutanoic acid (Fisher Scientific, Hampton, NH) and 1 mM acetyl-CoA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with 200 nM tetramethylrhodamine methyl ester (TMRM) (Invitrogen, Milan, Italy) for 10 minutes at 37°C and immediately measured on a FacsCalibur flow cytometer (Becton Dickinson ...
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bioRxiv - Plant Biology 2023Quote: ... and Methyl-PEG-Maleimide Reagent (MM(PEG)24) (Thermo Fisher, 22713). NEM was added to Chlamydomonas cultures growing as indicated in each case to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2023Quote: ... methyl tert-butyl ether and 2-propanol were from Thermo Fisher Scientific (Pittsburg ...
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bioRxiv - Cell Biology 2023Quote: Mitochondrial membrane potential was determined using tetramethylrhodamine methyl ester (TMRM) (Invitrogen). Cells were plated in 12-well plates and transfected with the indicated siRNAs for the indicated times ...
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bioRxiv - Immunology 2023Quote: ... 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (Thermo Fisher Scientific, cat.: 34042) was added and the reaction was terminated after 5 min under running tap water ...
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bioRxiv - Plant Biology 2024Quote: ... or X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside, W5376C; Thermo Fisher Scientific, Guilford, CT). GUS and/or LacZ-stained tissues were cleared for 30 s with 12 % sodium hypochlorite solution before microscopy observations.
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Neuroscience 2020Quote: ... were quantified in autaptic neuronal cultures using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43, Thermo Fisher Scientific, Waltham, MA, USA), similarly as in our previous report (Kawano et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... the medium was replaced with 5 ml fresh medium and 50 or 100 μM 12-azidodo-decanoate (azidomyristate) (Click-IT™ Myristic Acid, Azide, ThermoFisher Scientific, Cat n° C10268) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2019Quote: ... with different spectral signatures selected for the detection of PfHRP2 and PAN-pLDH were washed with distilled water and activated with Sulfo-NHS (N-hydroxysulfosuccinimide) and EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (Pierce, Thermo Fisher Scientific Inc., Rockford, IL), both at 50 mg/mL ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc., MA, USA) solution at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... All CAMP (Christie-Atkins-Munch-Peterson)-positive [22] bacterial isolates (n=58) were confirmed as GBS using a slide latex agglutination test (Streptex Latex Agglutination Test, ThermoFisher Scientific Inc., Waltham, MA, USA). GBS isolates were subcultured on blood agar to assess purity and inoculated on stab agars (SVA ...
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bioRxiv - Neuroscience 2022Quote: ... consisting of DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml 1% penicillin/ streptomycin (pen/strep; all Thermo Fisher Scientific, Waltham, MA, USA) + 20 ng/ml BDNF + 20 ng/ml GDNF + 20 ng/ml NGFb (all Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... collected as single cell suspensions using cell strainer and resuspended in sphere formation medium which was composed of 1:1 DMEM and F-12 nutrient mix supplemented with B-27 and N-2 (Thermo Fisher Scientific, Waltham, MA, USA). 1,000 cells were plated in each well of ultra-low attachment 6-well plates (Corning ...
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bioRxiv - Genetics 2022Quote: ... Over the course of the following 4 days, the colonies were gradually transitioned to neural induction medium (NIM) (consisting of DMEM/F12, 1% N-2 supplement (Fisher Scientific, Cat #: 17-502-048), MEM non-essential amino acids (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Mtb-SC and Mtb-AG-infected rabbit lungs (n=3 per group per time point) was isolated using TRIzol reagent (Life Technologies, Grand Island, NY, USA) as described earlier (5 ...